Modulation of hepatotoxicity, DNA fragmentation and gene expression of Solanum nigrum leaves extract in rats treated with silver nanoparticles

Objective: Synthesized silver nanoparticles (AgNPs) in liquids were investigated as anticancer cells in the present study. Cytotoxic activities of six different concentrations 0.78, 1.56, 3.125, 6.25, 12.5, and 25 μg/ml of AgNPs against human breast cancer cell line (AMJ13) and lymphocytes were assessed with MTT assay.Methods: A Q-Switched Nd: YAG pulsed laser (λ=1064 nm, 800 mJ/pulse) was used for ablation of a pure silver plate to synthesis AgNPs in the polyvinylpyrrolidone and deionize distilled water. Ultraviolet-visible spectroscopy confirmed the synthesis of AgNPs and zeta potential was evaluated. Morphology and size were analyzed by transmission electron microscope. AgNPs concentrations were determined by atomic absorption spectroscopy. Possibilities of apoptosis induction were confirmed using mitochondrial membrane potential assay, DNA fragmentation assay, and glutathione (GSH) assay.Results: The results indicated that AgNPs were able to induce an inhibition of AMJ13 cells compared their damaging effect toward normal lymphocytes were at minimal according to viability with MTT assay.. Furthermore, these results suggested that AgNPs-induced mitochondrial-mediated apoptosis cause DNA fragmentation, but no significant change in GSH level in AMJ13 cells.Conclusions: The overall results indicated that the physically synthesized AgNPs were exhibited dose-dependent cell death in AMJ13 breast cancer cell line, while the effect of AgNPs on lymphocytes was very low, suggesting that physically synthesized AgNPs might be a potential alternative agent for human breast cancer therapy.

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High-Content Imaging and Gene Expression Approaches To Unravel the Effect of Surface Functionality on Cellular Interactions of Silver Nanoparticles

ACS Nano ◽

10.1021/acsnano.5b04661 ◽

2015 ◽

Vol 9 (10) ◽

pp. 10431-10444 ◽

Cited By ~ 52

Author(s):

Bella B. Manshian ◽

Christian Pfeiffer ◽

Beatriz Pelaz ◽

Thomas Heimerl ◽

Marta Gallego ◽

...

Keyword(s):

Gene Expression ◽

Silver Nanoparticles ◽

Cellular Interactions ◽

High Content Imaging ◽

Surface Functionality ◽

Effect Of Surface

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Differential effects of silver nanoparticles on DNA damage and DNA repair gene expression in Ogg1-deficient and wild type mice

Nanotoxicology ◽

10.1080/17435390.2017.1388863 ◽

2017 ◽

Vol 11 (8) ◽

pp. 996-1011 ◽

Cited By ~ 17

Author(s):

Sameera Nallanthighal ◽

Cadia Chan ◽

Thomas M. Murray ◽

Aaron P. Mosier ◽

Nathaniel C. Cady ◽

...

Keyword(s):

Gene Expression ◽

Dna Damage ◽

Silver Nanoparticles ◽

Dna Repair ◽

Wild Type ◽

Repair Gene ◽

Differential Effects ◽

Dna Repair Gene

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In Ovo Administration of Silver Nanoparticles and/or Amino Acids Influence Metabolism and Immune Gene Expression in Chicken Embryos

International Journal of Molecular Sciences ◽

10.3390/ijms16059484 ◽

2015 ◽

Vol 16 (12) ◽

pp. 9484-9503 ◽

Cited By ~ 25

Author(s):

Subrat Bhanja ◽

Anna Hotowy ◽

Manish Mehra ◽

Ewa Sawosz ◽

Lane Pineda ◽

...

Keyword(s):

Gene Expression ◽

Amino Acids ◽

Silver Nanoparticles ◽

Immune Gene ◽

In Ovo ◽

Chicken Embryos ◽

Immune Gene Expression

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Toxicity of Silver Nanoparticles as Assessed by Global Gene Expression Analysis

Materials Express ◽

10.1166/mex.2011.1010 ◽

2011 ◽

Vol 1 (1) ◽

pp. 74-79 ◽

Cited By ~ 31

Author(s):

Liming Xu ◽

Taro Takemura ◽

Mingshen Xu ◽

Nobutaka Hanagata

Keyword(s):

Gene Expression ◽

Silver Nanoparticles ◽

Expression Analysis ◽

Gene Expression Analysis ◽

Global Gene Expression ◽

Global Gene Expression Analysis

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Synthesis and characterization of Solanum nigrum-mediated silver nanoparticles and its protective effect on alloxan-induced diabetic rats

Journal of Nanostructure in Chemistry ◽

10.1007/s40097-015-0178-6 ◽

2015 ◽

Vol 6 (1) ◽

pp. 41-48 ◽

Cited By ~ 29

Author(s):

Arumugam Sengottaiyan ◽

Adithan Aravinthan ◽

Chinnapan Sudhakar ◽

Kandasamy Selvam ◽

Palanisamy Srinivasan ◽

...

Keyword(s):

Silver Nanoparticles ◽

Protective Effect ◽

Diabetic Rats ◽

Solanum Nigrum ◽

Synthesis And Characterization

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Hypothyroidism alters the expression of Bcl-2 family genes to induce enhanced apoptosis in the developing cerebellum

Journal of Endocrinology ◽

10.1677/joe.0.1760039 ◽

2003 ◽

Vol 176 (1) ◽

pp. 39-46 ◽

Cited By ~ 42

Author(s):

R Singh ◽

G Upadhyay ◽

S Kumar ◽

A Kapoor ◽

A Kumar ◽

...

Keyword(s):

Gene Expression ◽

Dna Fragmentation ◽

Granule Cells ◽

Cerebellar Granule Cells ◽

Cell Loss ◽

Apoptotic Gene ◽

Euthyroid State ◽

Family Gene ◽

Apoptotic Genes ◽

High Level

Thyroid hormone (TH) deficiency results in delayed proliferation and migration of cerebellar granule cells. Although extensive cell loss during the development of the cerebellum under hypothyroid conditions is known, its nature and its mechanism are poorly understood. Bcl-2 family gene expression is known to determine the fate of cells to undergo apoptosis. We evaluated the effect of hypothyroidism on Bcl-2 family gene expression in the developing rat cerebellum. Electrophoresis and Western blotting were used to analyze DNA fragmentation and expression of DNA fragmentation factor (DFF-45), Bcl-2, Bcl-xL and Bax genes respectively. In the hypothyroid condition, extensive DNA fragmentation and enhanced cleavage of DFF-45 were seen throughout development (postnatal day 0 to day 24) and adulthood whereas they were absent in the euthyroid state. The anti-apoptotic genes Bcl-2 and Bcl-xL were down-regulated and the pro-apoptotic gene Bax was expressed at higher levels compared with the euthyroid state. These results suggest that normal levels of TH prevent cerebellar apoptosis to a large extent, whereas hypothyroidism not only increases the extent but also the duration of apoptosis by down-regulating the anti-apoptotic genes and maintaining a high level of the pro-apoptotic gene Bax.

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Silver nanoparticles induce the cardiomyogenic differentiation of bone marrow derived mesenchymal stem cells via telomere length extension

Beilstein Journal of Nanotechnology ◽

10.3762/bjnano.12.62 ◽

2021 ◽

Vol 12 ◽

pp. 786-797

Author(s):

Khosro Adibkia ◽

Ali Ehsani ◽

Asma Jodaei ◽

Ezzatollah Fathi ◽

Raheleh Farahzadi ◽

...

Keyword(s):

Gene Expression ◽

Stem Cells ◽

Bone Marrow ◽

Silver Nanoparticles ◽

Mesenchymal Stem Cells ◽

Telomere Length ◽

Cardiac Markers ◽

Ag Nps ◽

Cardiomyogenic Differentiation

Finding new strategies for the treatment of heart failures using stem cells has attracted a lot of attention. Meanwhile, nanotechnology-based approaches to regenerative medicine hypothesize a possible combination of stem cells and nanotechnology in the treatment of diseases. This study aims to investigate the in vitro effect of silver nanoparticles (Ag-NPs) on the cardiomyogenic differentiation of bone marrow-derived mesenchymal stem cells (BM-MSCs) through detection of cardiac markers. For this purpose, MSCs were isolated from bone marrow resident and differentiated to the cardiac cells using a dedicated medium with Ag-NPs. Also, the cardiomyogenic differentiation of BM-MSCs was confirmed using immunocytochemistry. Then, real-time PCR and western blotting assay were used for measuring absolute telomere length (TL) measurement, and gene and protein assessment of the cells, respectively. It was found that 2.5 µg/mL Ag-NPs caused elongation of the telomeres and altered VEGF, C-TnI, VWF, SMA, GATA-4, TERT, and cyclin D protein and gene expression in the cardiomyogenically differentiated BM-MSCs. Also, there was a significant increase in the protein and gene expression of Wnt3 and β-catenin as main components of pathways. We concluded that Ag-NPs could change the in vitro expression of cardiac markers of BM-MSCs via the Wnt3/β-catenin signaling pathway.

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Synthesis, Characterization of Silver Nanoparticles Using Nigella sativa Seeds and Study Their Effects on the Serum Lipid Profile and DNA Damage on the Rats’ Blood Treated with Hydrogen Peroxide

The Iraqi Journal of Veterinary Medicine ◽

10.30539/iraqijvm.v43i2.526 ◽

2019 ◽

Vol 43 (2) ◽

pp. 23-37

Author(s):

Zainab Sattar Ali

Keyword(s):

Hydrogen Peroxide ◽

Dna Damage ◽

Silver Nanoparticles ◽

Lipid Profile ◽

Dna Fragmentation ◽

Nigella Sativa ◽

Tap Water ◽

Male Rats ◽

High Dose ◽

Ameliorative Effect

This study was aimed to produce silver nanoparticles using aqueous extract of Nigella sativa, also to investigate the effects of green synthesized Nigella sativa seeds silver nanoparticles on dyslipidemia and DNA fragmentation in hydrogen peroxide-exposed rats. The produced Nigella sativa seeds silver nanoparticles were characterized through Ultraviolet-Visible spectroscopy, Fourier-transform infrared spectroscopy, X-ray powder diffraction (XRD) style, and Scanning Electron Microscope was used to investigate the morphology and size of synthesized Nigella sativa seeds silver nanoparticles. Forty adults male rats were randomly and equally divided into five groups and daily had been remedying for two months as followings: G1 group (Control), G2 group, rats in this group were received tap water containing 1% H2O2, animals in G3 and G4 groups were injected IP Nigella sativa seeds silver nanoparticles 25 and 50 mg/kg B.W., respectively, and received ordinary tap water containing 1% H2O2 , and G5 group, animals in this group were injected IP Nigella sativa seeds extract 50 mg/kg B.W. and received ordinary tap water containing 1% H2O2. Blood samples collected after one and two months of the experiment from each animal for DNA fragmentation measurements and serum estimated lipid profile.The results receded a case of dyslipidemia, as well significant elevation in DNA damage in G4 and G2 groups The results also confirmed the hypolipidemic and cytoprotective effect of Nigella sativa seeds extract (G5 group) and silver nanoparticles as25mg/kg B.W (group G3) clarified by correction of dyslipidemia, alongside significant alleviation in DNA damage. In conclusion, the results in the current study effectsof Nigella sativa seeds silver nanoparticles at high dose and documents the ameliorative effect of Nigella sativaseeds extract and Nigella sativa seeds silver nanoparticles on lipid profile and DNA damage.

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