scholarly journals In vitro cytotoxic activity assay of bacteria extract derived marine sponge Haliclona fascigera toward Hela, WiDr, T47D, and Vero cell line

2019 ◽  
Vol 9 (8) ◽  
pp. 66-70
Keyword(s):  
Cytotoxic Activity ◽  
Vero Cell ◽  
Cell Line ◽  
Marine Sponge ◽  
Activity Assay ◽  
Vero Cell Line

scholarly journals ​Synthesis, Characterization and Safety Assessment of Nano Selenium and Organic Selenium for Incorporation in Lamb Feed

2021 ◽  
Author(s):  
S.S. Vajpeyee ◽  
J. Ramesh ◽  
R. Karunakaran ◽  
J. Muralidharan ◽  
V. Sankar
Keyword(s):  
Particle Size ◽  
Vero Cell ◽  
Cell Line ◽  
Inductively Coupled Plasma ◽  
The Body ◽  
Nano Particles ◽  
Dependent Manner ◽  
Organic Selenium ◽  
Vero Cell Line

Background: Selenium is an important trace mineral required by the animals. It is an integral part of antioxidant system, protecting the body against free radical injury. Nano particles attract a widespread attention due to its high bioavailability and efficacy. The current study was aimed to synthesize, characterize nano selenium and evaluate the cytotoxic effect of nano selenium and organic selenium (selenocysteine) under in vitro condition in vero cell line. Methods: Nano selenium was synthesized by wet chemical method by using sodium selenite, selenium powder, ascorbic acid and sodium hydroxide at laboratory level. In this study particle size, shape, zeta potential and selenium content were characterized by using Particle Size Analyser (PSA), Transmission Electron Microscope (TEM) and Inductively Coupled Plasma Mass Spectrometry (ICP-MS). The toxicity was analysed by MTT assay against vero cell line. Result: The result revealed that selenium nano particles were spherical in shape, nano in size (less than 50 nm) and pure in nature. The nano selenium and organic selenium (selenocysteine) effectively inhibited the growth of vero cells in a dose dependent manner.

scholarly journals CYTOTOXICITY IDENTIFICATION OF THE SUPERNATANT OF Manihot esculenta Crantz RHIZOME AS RIBOSOME-INACTIVATING PROTEIN TO NORMAL CELL

2010 ◽  
Vol 4 (2) ◽  
pp. 77-81
Author(s):  
Endang Astuti ◽  
Sabirin Matsjeh ◽  
Winarto Haryadi ◽  
Deni Pranowo ◽  
Nuning Sri Mulatsih
Keyword(s):  
Cytotoxic Activity ◽  
Vero Cell ◽  
Cell Line ◽  
Cancer Cell ◽  
Manihot Esculenta ◽  
Normal Cell ◽  
Cytotoxic Effect ◽  
Ribosome Inactivating Protein ◽  
Manihot Esculenta Crantz ◽  
Vero Cell Line

The supernatant of Manihot esculenta Crantz rhizome has been used as an alternative remedy of infectious and cancer in Jogjakarta. Cytotoxicity assay showed that  Manihot esculenta Crantz supernatant had cytotoxic effect to cancer cell line, namely Myeloma (LC50 = 180,24 μg/mL) and HeLa (LC50 = 415,55 μg/mL), but have a little cytotoxic effect to SiHa. This research was aimed to identify cytotoxic activity of Manihot esculenta Crantz's supernatant to normal cell, particularly to human mononuclear cell and Vero cell line, and to compare to the cancer cell lines. The result showed that supernatant of M. esculenta had cytotoxic effect to normal mononuclear cell (LC50 = 564,00 μg/mL) and Vero cell line (LC50 = 686,00 μg/mL). The supernatant of M. esculenta had the highest cytotoxic activity to myeloma and relatively toxic to cervix cancer HeLa and normal cell, but less to SiHa.   Keywords: Manihot esculenta Crantz, Ribosome-inactivating Protein (RIP), cytotoxic, normal cell, cancer cell

scholarly journals Evaluation of Antiviral Activities of Curcumin Derivatives against HSV-1 in Vero Cell Line

2010 ◽  
Vol 5 (12) ◽  
pp. 1934578X1000501 ◽  
Author(s):  
Keivan Zandi ◽  
Elissa Ramedani ◽  
Khosro Mohammadi ◽  
Saeed Tajbakhsh ◽  
Iman Deilami ◽  
...  
Keyword(s):  
Cell Culture ◽  
Antiviral Activity ◽  
Vero Cell ◽  
Cell Line ◽  
Antiviral Agents ◽  
Antiviral Drug ◽  
In Vitro Study ◽  
Vero Cell Line ◽  
Hsv 1

Antiviral drug resistance is one of the most common problems in medicine, and, therefore, finding new antiviral agents, especially from natural resources, seems to be necessary. This study was designed to assay the antiviral activity of curcumin and its new derivatives like gallium-curcumin and Cu-curcumin on replication of HSV-1 in cell culture. The research was performed as an in vitro study in which the antiviral activity of different concentrations of three substances including curcumin, Gallium-curcumin and Cu-curcumin were tested on HSV-1. The cytotoxicity of the tested compounds was also evaluated on the Vero cell line. The CC50 values for curcumin, gallium-curcumin and Cu-curcumin were 484.2 μg/mL, 255.8 μg/mL and 326.6 μg/mL, respectively, and the respective IC50 values 33.0 μg/mL, 13.9 μg/mL and 23.1 μg/mL. The calculated SI values were 14.6, 18.4 and 14.1, respectively. The results showed that curcumin and its new derivatives have remarkable antiviral effects on HSV-1 in cell culture.

scholarly journals In Vitro Assessment of Gentamicin Cytotoxicity on the Selected Mammalian Cell Line (Vero cells)

2017 ◽  
Vol 1 (1) ◽  
pp. 111-116 ◽  
Author(s):  
Anton Kovacik ◽  
Eva Tvrda ◽  
Diana Fulopova ◽  
Peter Cupka ◽  
Eva Kovacikova ◽  
...  
Keyword(s):  
Cell Viability ◽  
Vero Cell ◽  
Cell Line ◽  
Mammalian Cell ◽  
Vero Cells ◽  
Mammalian Cell Line ◽  
Total Proteins ◽  
African Green Monkey Kidney ◽  
Vero Cell Line

Abstract The aim of this study was to evaluate the in vitro cytotoxicity of different concentrations (500-7500 μg/mL) of gentamicin - GENT (aminoglycoside antibiotic) on the selected mammalian cell line (Vero - cell line from African green monkey kidney). Analysis of the cell morphological changes was microscopically evaluated (magnification × 400). Quantification of Ca, Mg and total proteins was performed using spectrophotometry on device Rx Monza (Randox). Quantification of Na, K and Cl was performed on the automatic analyzer EasyLyte. The cell viability was assessed using the metabolic mitochondrial MTT test. Vero cells were able to survive at concentrations of 500 (89.21 %), 1000 (79.54 %) and 2000 μg/mL (34.59 %). We observed statistically significant decrease of vital cell content at concentrations of 2000, 4500, 7500 μg/mL against control group. Vero cell line slightly reacted to the presence of GENT but total proteins and mineral parameters were not significantly affected. Vero cells were highly sensitive to GENT with a significant decrease of viability at concentrations of 2000 and 4500 μg/mL (P < 0.001). Our data reveal that GENT has a significant cytotoxic and adverse effect on the cell viability.

scholarly journals In Vitro and In Silico Anti-Arboviral Activities of Dihalogenated Phenolic Derivates of L-Tyrosine

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3430
Author(s):  
Vanessa Loaiza-Cano ◽  
Laura Milena Monsalve-Escudero ◽  
Manuel Pastrana Restrepo ◽  
Diana Carolina Quintero-Gil ◽  
Sergio Andres Pulido Muñoz ◽  
...  
Keyword(s):  
Cell Line ◽  
Viral Protein ◽  
In Silico ◽  
Public Health Problem ◽  
Cellular Proteins ◽  
Vero Cell Line ◽  
Viral Particles ◽  
Pre Treatment ◽  
Line Infection

Despite the serious public health problem represented by the diseases caused by dengue (DENV), Zika (ZIKV) and chikungunya (CHIKV) viruses, there are still no specific licensed antivirals available for their treatment. Here, we examined the potential anti-arbovirus activity of ten di-halogenated compounds derived from L-tyrosine with modifications in amine and carboxyl groups. The activity of compounds on VERO cell line infection and the possible mechanism of action of the most promising compounds were evaluated. Finally, molecular docking between the compounds and viral and cellular proteins was evaluated in silico with Autodock Vina®, and the molecular dynamic with Gromacs®. Only two compounds (TDC-2M-ME and TDB-2M-ME) inhibited both ZIKV and CHIKV. Within the possible mechanism, in CHIKV, the two compounds decreased the number of genome copies and in the pre-treatment strategy the infectious viral particles. In the ZIKV model, only TDB-2M-ME inhibited the viral protein and demonstrate a virucidal effect. Moreover, in the U937 cell line infected with CHIKV, both compounds inhibited the viral protein and TDB-2M-ME inhibited the viral genome too. Finally, the in silico results showed a favorable binding energy between the compounds and the helicases of both viral models, the NSP3 of CHIKV and cellular proteins DDC and β2 adrenoreceptor.

Whole Genome Sequencing and Phylogenetic Analysis of the Rabies Virus Strain Moscow 3253 Adapted to a Vero Cell Line

2020 ◽  
Vol 35 (4) ◽  
pp. 237-242
Author(s):  
Ya. M. Krasnov ◽  
Zh. V. Alkhova ◽  
S. V. Generalov ◽  
I. V. Tuchkov ◽  
E. A. Naryshkina ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Vero Cell ◽  
Whole Genome Sequencing ◽  
Cell Line ◽  
Rabies Virus ◽  
Genome Sequencing ◽  
Virus Strain ◽  
Whole Genome ◽  
Vero Cell Line ◽  
Rabies Virus Strain
Sign in / Sign up

Export Citation Format

Share Document