scholarly journals tRNA sequences can assemble into a replicator

eLife ◽  
2021 ◽  
Vol 10 ◽  
Author(s):  
Alexandra Kühnlein ◽  
Simon A Lanzmich ◽  
Dieter Braun
Keyword(s):  
Genetic Code ◽  
Self Assembly ◽  
Molecular Assembly ◽  
Transfer Rna ◽  
Binary Sequences ◽  
Replication Fidelity ◽  
Dna Hairpins ◽  
Single Mechanism ◽  
Thermal Oscillations ◽  
Molecular Replication

Can replication and translation emerge in a single mechanism via self-assembly? The key molecule, transfer RNA (tRNA), is one of the most ancient molecules and contains the genetic code. Our experiments show how a pool of oligonucleotides, adapted with minor mutations from tRNA, spontaneously formed molecular assemblies and replicated information autonomously using only reversible hybridization under thermal oscillations. The pool of cross-complementary hairpins self-selected by agglomeration and sedimentation. The metastable DNA hairpins bound to a template and then interconnected by hybridization. Thermal oscillations separated replicates from their templates and drove an exponential, cross-catalytic replication. The molecular assembly could encode and replicate binary sequences with a replication fidelity corresponding to 85–90 % per nucleotide. The replication by a self-assembly of tRNA-like sequences suggests that early forms of tRNA could have been involved in molecular replication. This would link the evolution of translation to a mechanism of molecular replication.

scholarly journals Identification and nanomechanical characterization of the fundamental single-strand protofilaments of amyloid α-synuclein fibrils

2018 ◽  
Vol 115 (28) ◽  
pp. 7230-7235 ◽  
Author(s):  
Francesco Simone Ruggeri ◽  
Fabrizio Benedetti ◽  
Tuomas P. J. Knowles ◽  
Hilal A. Lashuel ◽  
Sergey Sekatskii ◽  
...  
Keyword(s):  
Single Molecule ◽  
Self Assembly ◽  
Amyloid Fibrils ◽  
Molecular Assembly ◽  
Single Strand ◽  
Force Microscopy ◽  
Atomic Force ◽  
Presynaptic Protein ◽  
Polypeptide Chains

The formation and spreading of amyloid aggregates from the presynaptic protein α-synuclein in the brain play central roles in the pathogenesis of Parkinson’s disease. Here, we use high-resolution atomic force microscopy to investigate the early oligomerization events of α-synuclein with single monomer angstrom resolution. We identify, visualize, and characterize directly the smallest elementary unit in the hierarchical assembly of amyloid fibrils, termed here single-strand protofilaments. We show that protofilaments form from the direct molecular assembly of unfolded monomeric α-synuclein polypeptide chains. To unravel protofilaments’ internal structure and elastic properties, we manipulated nanomechanically these species by atomic force spectroscopy. The single-molecule scale identification and characterization of the fundamental unit of amyloid assemblies provide insights into early events underlying their formation and shed light on opportunities for therapeutic intervention at the early stages of aberrant protein self-assembly.

scholarly journals Transfer RNA: From minihelix to genetic code

Cell ◽  
1995 ◽  
Vol 81 (7) ◽  
pp. 983-986 ◽  
Author(s):  
Paul Schimmel ◽  
Lluís Ribas de Pouplana
Keyword(s):  
Genetic Code ◽  
Transfer Rna

Orientation of Ferroelectric Liquid Crystals by Self-Assembled Bilayer Molecular Assembly

2001 ◽  
Vol 709 ◽  
Author(s):  
Sukhmal C. Jain ◽  
Vivechana Dixit ◽  
Vinod K. Tanwar ◽  
S. M. Shivaprasad
Keyword(s):  
Liquid Crystal ◽  
Indium Tin Oxide ◽  
Photoelectron Spectroscopy ◽  
Self Assembly ◽  
Uv Light ◽  
Uv Spectroscopy ◽  
Large Change ◽  
Molecular Assembly ◽  
Ferroelectric Liquid Crystal ◽  
Assembly Technique

ABSTRACTMolecular films of amino propyl triethoxy silane (APTES) attached with cinnamoyl moieties were deposited on indium tin oxide (ITO) coated glass and quartz substrates by self-assembly technique. The deposition of the monolayer films resulted in a large change in the wettability of the substrates. X-ray photoelectron spectroscopy (XPS) and UV-spectroscopy were used to study the structure and properties of the grown films. The molecular assembly was photodimerized by irradiating it with a linearly polarized UV-light. Thus created polymer-solid interface has been shown to produce good, stable planar orientation of ferroelectric liquid crystal mixtures. The electro-optical and switching properties of the ferroelectric liquid crystal cells have been investigated and found to be quite similar to those prepared in conventional manner.

scholarly journals Molecular Assembly and Self-Assembly: Molecular Nanoscience for Future Technologies

2002 ◽  
Vol 56 (10) ◽  
pp. 500-505 ◽  
Author(s):  
Michael de Wild ◽  
Simon Berner ◽  
Hitoshi Suzuki ◽  
Luca Ramoino ◽  
Alexis Baratoff ◽  
...  
Keyword(s):  
Self Assembly ◽  
Molecular Assembly ◽  
Future Technologies

scholarly journals Graphene transistors for real-time monitoring molecular self-assembly dynamics

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Marco Gobbi ◽  
Agostino Galanti ◽  
Marc-Antoine Stoeckel ◽  
Bjorn Zyska ◽  
Sara Bonacchi ◽  
...  
Keyword(s):  
Real Time ◽  
Self Assembly ◽  
Molecular Assembly ◽  
Self Organization ◽  
Highly Sensitive ◽  
Graphene Devices ◽  
Solid Liquid Interface ◽  
Solid Liquid ◽  
Molecular Ensemble

Abstract Mastering the dynamics of molecular assembly on surfaces enables the engineering of predictable structural motifs to bestow programmable properties upon target substrates. Yet, monitoring self-assembly in real time on technologically relevant interfaces between a substrate and a solution is challenging, due to experimental complexity of disentangling interfacial from bulk phenomena. Here, we show that graphene devices can be used as highly sensitive detectors to read out the dynamics of molecular self-assembly at the solid/liquid interface in-situ. Irradiation of a photochromic molecule is used to trigger the formation of a metastable self-assembled adlayer on graphene and the dynamics of this process are monitored by tracking the current in the device over time. In perspective, the electrical readout in graphene devices is a diagnostic and highly sensitive means to resolve molecular ensemble dynamics occurring down to the nanosecond time scale, thereby providing a practical and powerful tool to investigate molecular self-organization in 2D.

scholarly journals A Raman Spectroscopic and Computational Study of New Aromatic Pyrimidine-Based Halogen Bond Acceptors

Inorganics ◽  
2019 ◽  
Vol 7 (10) ◽  
pp. 119 ◽  
Author(s):  
Hardin ◽  
Ellington ◽  
Nguyen ◽  
Rheingold ◽  
Tschumper ◽  
...  
Keyword(s):  
Self Assembly ◽  
Density Functional ◽  
Computational Study ◽  
Halogen Bond ◽  
Normal Modes ◽  
Halogen Bonding ◽  
Building Blocks ◽  
Molecular Assembly ◽  
X Ray ◽  
X Ray Crystallography

Two new aromatic pyrimidine-based derivatives designed specifically for halogen bond directed self-assembly are investigated through a combination of high-resolution Raman spectroscopy, X-ray crystallography, and computational quantum chemistry. The vibrational frequencies of these new molecular building blocks, pyrimidine capped with furan (PrmF) and thiophene (PrmT), are compared to those previously assigned for pyrimidine (Prm). The modifications affect only a select few of the normal modes of Prm, most noticeably its signature ring breathing mode, ν1. Structural analyses afforded by X-ray crystallography, and computed interaction energies from density functional theory computations indicate that, although weak hydrogen bonding (C–H···O or C–H···N interactions) is present in these pyrimidine-based solid-state co-crystals, halogen bonding and π-stacking interactions play more dominant roles in driving their molecular-assembly.

Transfer RNA paralogs: evidence for genetic code-amino acid biosynthesis coevolution and an archaeal root of life

Gene ◽  
2003 ◽  
Vol 310 ◽  
pp. 59-66 ◽  
Author(s):  
Hong Xue ◽  
Ka-Lok Tong ◽  
Christian Marck ◽  
Henri Grosjean ◽  
J.Tze-Fei Wong
Keyword(s):  
Amino Acid ◽  
Genetic Code ◽  
Amino Acid Biosynthesis ◽  
Transfer Rna ◽  
Acid Biosynthesis

scholarly journals Molecular Assembly and Self-Assembly: Molecular Nanoscience for Future Technologies

2003 ◽  
Vol 1006 (1) ◽  
pp. 291-305 ◽  
Author(s):  
M WILD ◽  
S BERNER ◽  
H SUZUKI ◽  
L RAMOINO ◽  
A BARATOFF ◽  
...  
Keyword(s):  
Self Assembly ◽  
Molecular Assembly ◽  
Future Technologies

Fast naked-eye detection of zinc ions by molecular assembly-assisted polymerization of diacetylene

Nanoscale ◽  
2018 ◽  
Vol 10 (39) ◽  
pp. 18829-18834 ◽  
Author(s):  
Yiming Zhang ◽  
Yu-Chen Pan ◽  
Youzhi Wang ◽  
Dong-Sheng Guo ◽  
Jie Gao ◽  
...  
Keyword(s):  
Self Assembly ◽  
Molecular Assembly ◽  
Zinc Ions ◽  
Eye Detection ◽  
Ion Detection ◽  
Naked Eye ◽  
Topochemical Polymerization ◽  
Detection Technology ◽  
New Strategy ◽  
Naked Eye Detection

A new strategy for developing portable rapid ion detection technology by the coupling of topochemical polymerization and supramolecular (SM) self-assembly.

The Preparation of Comb Polymer Rotaxanes and Study on their Self-Assembly Behavior

2016 ◽  
Vol 852 ◽  
pp. 653-656
Author(s):  
Li Sun ◽  
Lin Zhu ◽  
Jun Peng Xie ◽  
Rui Fang Guan
Keyword(s):  
Self Assembly ◽  
Inclusion Compound ◽  
Life Science ◽  
Molecular Assembly ◽  
The Self ◽  
X Ray Diffraction ◽  
X Ray ◽  
H Nmr ◽  
Self Assembling ◽  
Assembly Behavior

It is very important to form a stable, structure-controlled material by molecular assembly inthe fields of materials and life science. How cyclodextrins affect the self–assembly behavior of inclusion compounds becomes the focus recently, due to the advantages of selectivity, regulating, capability, biocompatibility and other interesting properties. In thispaper, polyoxyethylene ω-tristyrylphenyl methacrylate (SEM-25) and α-cyclodextrin (α-CD) form inclusion compound (AS) firstly, then copolymer of AS and methyl methacrylate (MMA) - the comb-like polyrotaxanes (CD-PR) were prepared through the redox initiationsystem of free radical polymerization. Furthermore, the self-assembling behavior of CD-PR is studied, it is found that solvent has an great impact on aggregation morphology of CD-PR without changing composition of the polyrotaxanes. Products were characterized by X-ray diffraction (XRD), nuclear magnetic resonance (1H NMR) and scanning electron microscope (SEM).

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