Combined Enzymatic Pretreatment of Pulp for Production of CNF

2021 ◽  
Vol 53 (5) ◽  
pp. 5-15
Author(s):  
Shin Young Park ◽  
Seokho Lee ◽  
Wanhee Im ◽  
Hak Lae Lee ◽  
Hye Jung Youn
Keyword(s):  
Enzymatic Pretreatment

scholarly journals Role of laccase as an enzymatic pretreatment method to improve lignocellulosic saccharification

2014 ◽  
Vol 4 (8) ◽  
pp. 2251-2259 ◽  
Author(s):  
Lucy Heap ◽  
Anthony Green ◽  
David Brown ◽  
Bart van Dongen ◽  
Nicholas Turner
Keyword(s):  
Wheat Straw ◽  
Trametes Versicolor ◽  
Enzymatic Pretreatment ◽  
Pretreatment Method ◽  
Alkaline Peroxide

The saccharification of wheat straw was improved when an incubation step was performed withTrametes versicolorlaccase (TvL) and the mediator 1-hydroxybenzotriazole (1-HBT) prior to an alkaline peroxide extraction (APE).

Integration of enzymatic pretreatment and sludge co-digestion in biogas production from microalgae

2021 ◽  
Vol 124 ◽  
pp. 254-263
Author(s):  
Romina Avila ◽  
Elvira Carrero ◽  
Teresa Vicent ◽  
Paqui Blánquez
Keyword(s):  
Biogas Production ◽  
Enzymatic Pretreatment

Effect of enzymatic pretreatment on solubilization and volatile fatty acid production in fermentation of food waste

2005 ◽  
Vol 52 (10-11) ◽  
pp. 51-59 ◽  
Author(s):  
H.J. Kim ◽  
Y.G. Choi ◽  
G.D. Kim ◽  
S.H. Kim ◽  
T.H. Chung
Keyword(s):  
Fatty Acid ◽  
Food Waste ◽  
Volatile Fatty Acid ◽  
Biological Nutrient Removal ◽  
High Concentration ◽  
Acid Fermentation ◽  
Mixture Ratio ◽  
Enzymatic Pretreatment ◽  
Enzyme Mixture ◽  
Enzyme Dosage

Food waste can be a valuable carbon source in biological nutrient removal (BNR) systems because of the high C/N and C/P ratio. However, pretreatment is necessary to promote hydrolysis of food waste because of the high concentration of volatile solids associated with organic matter. The influence of the enzymatic pretreatment on acid fermentation of food waste was investigated in this study. Solubilization of particulate matter in food waste was carried out using commercial enzymes. The acidification efficiency and the volatile fatty acid (VFA) production potential of enzymatically pretreated food waste were examined. The highest volatile suspended solid (VSS) reduction was obtained with an enzyme mixture ratio of 1: 2: 1 for carbohydrase: protease: lipase. An optimum enzyme dosage for solubilization of food waste was 0.1% (V/V) with the enzyme mixture ratio of 1: 2: 1. In the acid fermen- tation of enzymatically pretreated food waste, the maximum VFA production and the highest VFA fraction in soluble COD (SCOD) were also achieved at 0.1% (V/V) of total enzyme dosage. Increase in VFA production at this level of enzyme dosage was over 300% compared with the control fermenter. The major form of VFA produced by fermentation was n-butyrate followed by acetate.

Impact of enzymatic pretreatment on corn stover degradation and biogas production

2014 ◽  
Vol 173 ◽  
pp. 59-66 ◽  
Author(s):  
Michel Schroyen ◽  
Han Vervaeren ◽  
Stijn W.H. Van Hulle ◽  
Katleen Raes
Keyword(s):  
Corn Stover ◽  
Biogas Production ◽  
Enzymatic Pretreatment

Effect of cellulase-assisted refining on the thermal degradation of bleached high-density paper

Holzforschung ◽  
2015 ◽  
Vol 69 (6) ◽  
pp. 703-712 ◽  
Author(s):  
Emilia Vänskä ◽  
Tapani Vuorinen
Keyword(s):  
Tensile Strength ◽  
Thermal Degradation ◽  
Specific Energy Consumption ◽  
Degree Of Polymerization ◽  
High Density ◽  
Pulp Fibers ◽  
Enzymatic Pretreatment ◽  
Burst Strength ◽  
Degradation Reactions ◽  
Cellulase Treatment

Abstract Pulp was treated with cellulase, and the chemical, physical, and optical properties of the refined pulps in paper sheets were measured in terms of the degree of polymerization of cellulose, tensile strength, elongation, burst strength, light scattering, and brightness. The sheets were thermally treated for 20 and 60 min at 225°C in the presence of 1% and 75% (v/v) water vapor. The cellulase treatment intensified the fibrillation of fibers and reduced the specific energy consumption during refining. It was demonstrated based on the water retention value that the refining modified the water-bonding ability of the pulp fibers, which was further enhanced in the case of cellulase-treated pulps. However, the enzymatic pretreatment impaired the physical properties, such as the tensile strength, elongation, and burst strength of the high-density paper. These changes further reduced the thermal strength stability of the paper. In addition, considerable brightness loss of the cellulase-treated high-density paper was observed compared to the reference paper, indicating more severe thermal degradation reactions as a consequence of enzyme-assisted refining.

scholarly journals An Efficient Method for Cellulose Nanofibrils Length Shearing via Environmentally Friendly Mixed Cellulase Pretreatment

2017 ◽  
Vol 2017 ◽  
pp. 1-12 ◽  
Author(s):  
Yuan Chen ◽  
Yuchan He ◽  
Dongbin Fan ◽  
Yanming Han ◽  
Gaiyun Li ◽  
...  
Keyword(s):  
Cellulose Nanofibrils ◽  
Trichoderma Viride ◽  
Cellulose Fibers ◽  
Effective Diameter ◽  
Layer By Layer ◽  
Mechanical Grinding ◽  
Hydrolysis Time ◽  
Sem Images ◽  
Enzymatic Pretreatment ◽  
Cellulase Hydrolysis

Cellulose nanofibrils (CNFs) have potential applications in the development of innovative materials and enhancement of conventional materials properties. This paper focused on the mixed cellulase hydrolysis with major activity of exoglucanase and endoglucanase on the cellulose length shearing. By the cooperation of two-step production route, including (1) enzymatic pretreatment using cellulase fromTrichoderma virideand (2) mechanical grinding twice, a shorter cellulose nanofiber was fabricated. The influence of enzymatic charge and hydrolysis time on cellulose fibers was analyzed by using scanning electron microscopy (SEM), Fourier Transform Infrared Spectrometer (FTIR), and X-ray diffractometer (XRD). SEM images revealed that the surface morphology change, effective diameter sharpening, and length shearing of cellulose fibers are as a result of cellulase hydrolysis. The XRD suggested that the cellulase acted on the amorphous regions more strongly than the crystalline domains during layer-by-layer hydrolysis. The enzymatic charge and hydrolysis time significantly affected the yields and hydrolysis products concentration. The enzymatic pretreatment assisted mechanical grinding could improve the uniformity of CNF and helped to obtain CNF with exact length according to the requirement for special applications.

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