COI barcoding of plant bugs (Insecta: Hemiptera: Miridae)

The family Miridae is the most diverse and one of the most economically important groups in Heteroptera. However, identification of mirid species on the basis of morphology is difficult and time-consuming. In the present study, we evaluated the effectiveness of COI barcoding for 123 species of plant bugs in seven subfamilies. With the exception of threeApolygusspecies—A. lucorum,A. spinolae, andA. watajii(subfamily Mirinae)—each of the investigated species possessed a unique COI sequence. The average minimum interspecific genetic distance of congeners was approximately 37 times higher than the average maximum intraspecific genetic distance, indicating a significant barcoding gap. Despite having distinct morphological characters,A. lucorum,A. spinolae, andA. watajiimixed and clustered together, suggesting taxonomic revision. Our findings indicate that COI barcoding represents a valuable identification tool for Miridae and can be economically viable in a variety of scientific research fields.

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A taxonomic revision of the family Harrimaniidae (Hemichordata: Enteropneusta) with descriptions of seven species from the Eastern Pacific

Zootaxa ◽

10.11646/zootaxa.2408.1.1 ◽

2010 ◽

Vol 2408 (1) ◽

pp. 1 ◽

Cited By ~ 12

Author(s):

C. DELAND ◽

C. B. CAMERON ◽

K. P. RAO ◽

W. E. RITTER ◽

T. H. BULLOCK

Keyword(s):

New Species ◽

Taxonomic Revision ◽

Morphological Characters ◽

Eastern Pacific ◽

Nomen Nudum ◽

Phylogenetic Hypothesis ◽

Number Of Species ◽

Dichotomous Key ◽

The Family ◽

The Status

The family Harrimaniidae (Hemichordata: Enteropneusta) is revised on the basis of morphological characters. The number of harrimaniid genera is increased to nine by the addition of Horstia n. gen., Mesoglossus n. gen., Ritteria n. gen. and Saxipendium, a genus previously assigned to the monospecific family Saxipendiidae. The number of species is increased to 34, resulting from the description of five new species from the eastern Pacific — Horstia kincaidi, Mesoglossus intermedius, M. macginitiei, Protoglossus mackiei and Ritteria ambigua. A description is supplied for a sixth harrimaniid species, Stereobalanus willeyi Ritter & Davis, 1904, which previously had the status of a nomen nudum. Four harrimaniids previously assigned to the genus Saccoglossus are transfered to the genus Mesoglossus — M. bournei, M. caraibicus, M. gurneyi and M. pygmaeus, while Saccoglossus borealis is reassigned to the genus Harrimania. Notes on habitat and zoogeography are included for the seven foregoing species and a table of diagnostic characters for existing and new species and a dichotomous key to the enteropneust families and harrimaniid genera are provided. Finally, a phylogenetic hypothesis concerning the Harrimaniidae is postulated, with discussion on the evolution of the group.

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New records and their associated DNA barcodes of the butterfly family Hesperiidae in Tibet, China

Zootaxa ◽

10.11646/zootaxa.4674.4.2 ◽

2019 ◽

Vol 4674 (4) ◽

pp. 426-438

Author(s):

CHENGLONG CAO ◽

SIYAO HUANG ◽

YONGQIANG XU ◽

HAOMIN WU ◽

TIANPENG CHEN ◽

...

Keyword(s):

Genetic Distance ◽

Morphological Study ◽

New Records ◽

Coi Gene ◽

Dna Barcodes ◽

Species Cluster ◽

The Family ◽

Interspecific Genetic Distance ◽

Coi Sequences ◽

First Time

The specimens of the family Hesperiidae collected from Tibet during 2016–2018 are identified using morphology. COI sequences of 76 individuals are newly obtained. The result of our morphological study is congruent with COI gene analyses. Maximum likehood (ML) and Bayesina inferences (BI) analyses reveal that individuals identified morphologically as the same species cluster cohesively. The minimum interspecific genetic distance is 1.7% between Halpe aucma and H. filda, and the genetic distance between conspecific individuals ranged from 0 to 0.2% for the genus Halpe. A total of 51 species are recognized, and six of them, Celaenorrhinus consanguineus Leech, 1891, Barca bicolor (Oberthür, 1896), Aeromachus propinquus Alphéraky, 1897, Pedesta bivitta (Oberthür, 1886), Baoris penicillata chapmani Evans, 1937, and Ochlodes brahma Moore, 1878, are reported from Tibet for the first time, and the last species is new to China.

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Corrigendum to: Systematics and distribution of world hyptiogastrine wasps (Hymenoptera : Gasteruptiidae)

Invertebrate Systematics ◽

10.1071/it01048_co ◽

2002 ◽

Vol 16 (6) ◽

pp. 957 ◽

Cited By ~ 1

Author(s):

J. T. Jennings ◽

A. D. Austin

Keyword(s):

New Species ◽

New Zealand ◽

South America ◽

New Caledonia ◽

Cladistic Analysis ◽

Taxonomic Revision ◽

Morphological Characters ◽

The Family ◽

New Hebrides ◽

New Britain

This study examines the phylogeny, taxonomy, distribution and biology of the gasteruptiid subfamily Hyptiogastrinae and, at the same time, presents an overview of the family. Following a cladistic analysis of 35 discrete morphological characters, two monophyletic genera are recognised, Hyptiogaster Kieffer and Pseudofoenus s. l. Kieffer. As a consequence, the genera Aulacofoenus Kieffer, Crassifoenus Crosskey, and Eufoenus Szépligeti are synonymised with Pseudofoenus. A total of 88 species are recognised for the subfamily, 10 species of Hyptiogaster, which are restricted to mainland Australia, and 78 species of Pseudofoenus, 40 of which are described as new. Pseudofoenus has a restricted Gondwanan distribution and is found in Australia including Tasmania (65 spp.), New Guinea and New Britain (5 spp.), the south-west Pacific (New Caledonia, New Hebrides and Fiji – 2 spp.), New Zealand (4 spp.) and South America (2 spp.). No new species have been recorded from either New Zealand or South America. For Pseudofoenus, information on the distribution of each species, their biology (if known) and an identification key are presented.Following a taxonomic revision, the following new species are described: P. baileyi, sp. nov., P. baitetaensis, sp. nov., P. beverlyae, sp. nov., P. caperatus, sp. nov., P. cardaleae, sp. nov., P. carrabinensis, sp. nov., P. claireae, sp. nov., P. collessi, sp. nov., P. coorowensis, sp. nov., P. crosskeyi, sp. nov., P. douglasorum, sp. nov., P. eliseae, sp. nov., P. ericae, sp. nov., P. eustonensis, sp. nov., P. feckneri, sp. nov., P. gressitti, sp. nov., P. gullanae, sp. nov., P. hackeri, sp. nov., P. imbricatus, sp. nov., P. iqbali, sp. nov., P. kadowi, sp. nov., P. karimuiensis, sp. nov., P. kelleri, sp. nov., P. leinsterensis, sp. nov., P. macdonaldi, sp. nov., P. malkini, sp. nov., P. marshalli, sp. nov., P. masneri, sp. nov., P. mitchellae, sp. nov., P. morganensis, sp. nov., P. nalbarraensis, sp. nov., P. pumilis, sp. nov., P. schmidti, sp. nov., P. stevensi, sp. nov., P. tasmaniensis, sp. nov., P. taylori, sp. nov., P. umboiensis, sp. nov., P. walkeri, sp. nov. and P. zborowskii, sp. nov. The synonymy of Aulacofoenus, Crassifoenus and Eufoenus with Pseudofoenus result in the following new combinations: from Aulacofoenus: P. bungeyi (Jennings & Austin), comb. nov., P. deletangi (Schletterer), comb. nov., P. fallax (Schletterer), comb. nov., P. fletcheri (Jennings & Austin), comb. nov., P. goonooensis (Jennings & Austin), comb. nov., P. infumatus (Schletterer), comb. nov., P. kurmondi (Jennings & Austin), comb. nov., P. loxleyi (Jennings & Austin), comb. nov., P. marionae (Jennings & Austin), comb. nov., P. perenjorii (Jennings & Austin), comb. nov., P. swani (Jennings & Austin), comb. nov., P. thoracicus (Guérin Menéville), comb. nov., P. whiani (Jennings & Austin), comb. nov. and P. wubinensis (Jennings & Austin), comb. nov.; from Crassifoenus: P. houstoni (Jennings & Austin), comb. nov., P. grossitarsis (Kieffer), comb. nov and P. macronyx (Schletterer), comb. nov.; and from Eufoenus: P. antennalis (Schletterer), comb. nov., P. australis (Westwood), comb. nov., P. crassitarsis (Kieffer), comb. nov., P. darwini (Westwood), comb. nov., P. extraneus (Turner), comb. nov., P. ferrugineus (Crosskey), comb. nov., P. floricolus (Turner), comb. nov., P. inaequalis (Turner), comb. nov., P. melanopleurus (Crosskey), comb. nov., P. minimus (Turner), comb. nov., P. nitidiusculus (Turner), comb. nov., P. patellatus (Westwood), comb. nov., P. pilosus (Kieffer), comb. nov., P. reticulatus (Crosskey), comb. nov., P. rieki (Crosskey), comb. nov., P. ritae (Cheesman), comb. nov. and P. spinitarsis (Westwood), comb. nov. Pseudofoenus microcephalus (Crosskey), comb. nov. is transferred from Hyptiogaster and Eufoenus flavinervis (Kieffer) remains incertae sedis.

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A taxonomic evaluation of the Thesium confine species complex (Santalaceae)

Bothalia ◽

10.4102/abc.v48i1.2346 ◽

2018 ◽

Vol 48 (1) ◽

Cited By ~ 1

Author(s):

Kagiso S. Mashego ◽

M. Marianne Le Roux

Keyword(s):

Species Complex ◽

Growth Form ◽

Taxonomic Revision ◽

Morphological Characters ◽

Research Strategy ◽

Herbarium Specimens ◽

African Species ◽

Separate Species ◽

Distribution Ranges ◽

The Family

Background: Thesium L. is the largest genus in the family Santalaceae (sandalwood family). The last taxonomic revision of the southern African species dates back to 1925. An urgent revision of the genus is required as indicated in a recent national biosystematics research strategy for South Africa.Objectives: To revise the Thesium confine species complex (Thesium durum, T. confine and Thesium spartioides) and to update the typification, nomenclature, descriptions and distribution ranges.Method: Morphological characters were studied using own field collections as well as herbarium specimens from K, NU, PRE and PRU and images on Global Plants (JSTOR). Distribution ranges of the studied species were updated.Results: An examination of morphological characters revealed great similarity between T. spartioides and T. confine. Both species have an herbaceous habit, decumbent growth form, terminal (or less frequently axillary) inflorescences and a sympatric northern distribution in South Africa. Thesium durum differs from T. spartioides and T. confine in having a shrubby habit, erect growth form and axillary inflorescences and occurs in the southern part of South Africa.Conclusion: Thesium spartioides is synonymised under T. confine, and T. durum is retained as a separate species.

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Phylogeny and taxonomic revision of the spider mite genera Aponychus, Paraponychus and Stylophoronychus using morphology (Acari : Tetranychidae)

Invertebrate Systematics ◽

10.1071/is12071 ◽

2013 ◽

Vol 27 (3) ◽

pp. 265 ◽

Cited By ~ 1

Author(s):

Fabio A. Hernandes ◽

Reinaldo J. F. Feres

Keyword(s):

Phylogenetic Analysis ◽

Spider Mite ◽

Cladistic Analysis ◽

Taxonomic Revision ◽

Morphological Characters ◽

Cultivated Plants ◽

Monophyletic Clade ◽

Taxonomic Review ◽

The Family

Despite their great importance as pests of cultivated plants worldwide, members of the family Tetranychidae have never been subjected to a thorough cladistic analysis to reveal the history and relationships among related genera. Herein, we provide the first phylogenetic analysis and taxonomic review of species of the genera Aponychus, Paraponychus and Stylophoronychus using morphological characters. The results indicate a monophyletic clade uniting the aforementioned genera, although none of the three genera were recovered as monophyletic. We reinstate the tribe Aponychini as the taxon containing those three genera. Aponychus bambusae and A. aequilibris are herein considered junior synonyms of Stylophoronychus vannus and A. corpuzae, respectively.

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Molecular phylogeny of Acrobeloides and Cephalobus (Nematoda: Cephalobidae) reveals paraphyletic taxa and recurrent evolution of simple labial morphology

Nematology ◽

10.1163/156854106779799178 ◽

2006 ◽

Vol 8 (6) ◽

pp. 819-836 ◽

Cited By ~ 16

Author(s):

Steven Nadler ◽

Ashleigh Smythe

Keyword(s):

Molecular Phylogeny ◽

Large Subunit ◽

Taxonomic Revision ◽

Morphological Characters ◽

Lateral Field ◽

The Core ◽

Morphological Taxonomy ◽

Dominant Feature ◽

The Family ◽

Molecular Phylogenetic

AbstractMembers of the family Cephalobidae (Nematoda) are among the most common and morphologically striking soil nematodes. Many members of Cephalobidae have extensive lip elaborations called probolae, but two taxonomically problematic genera, Acrobeloides and Cephalobus, have simple, low probolae. We sequenced a portion of the nuclear large subunit ribosomal DNA for 33 cultures of Acrobeloides and Cephalobus. A phylogenetic analysis of these data, plus sequences representing other members of Cephalobina, revealed a core clade of 22 closely related taxa, but did not represent Acrobeloides and Cephalobus as monophyletic. The dominant feature used in morphological taxonomy of Cephalobidae, the lip region, was homoplastic according to the molecular phylogenetic hypothesis. Contrary to previous suggestions, taxa with simple probolae have arisen multiple times from taxa with complex probolae. Cultures were also examined for mode of reproduction (presumed parthenogenetic vs sexual) and three morphological characters commonly used in generic diagnoses: the shape of the corpus in profile; the number of lateral incisures; and the terminal extent of the lateral field. Most cultures, including all 22 members of the core clade, lacked males and were presumed to be parthenogenetic, but several independent origins of sexually reproducing taxa were found. Of the morphological characters, only the corpus shape was consistent with the molecular phylogeny, however, the utility of this character is also questioned. Many genera with complex probolae were also paraphyletic, including Nothacrobeles, Zeldia and Cervidellus, indicating the need for more comprehensive phylogenies and a broad taxonomic revision of the family.

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Illuminating species diversity of nudibranch in Indonesian coral reef ecosystem using molecular identification

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/944/1/012033 ◽

2021 ◽

Vol 944 (1) ◽

pp. 012033

Author(s):

I G W D Dharmawan ◽

D G Bengen ◽

I Setyobudiandi ◽

B Subhan ◽

I Verawati ◽

...

Keyword(s):

Species Diversity ◽

Genetic Distance ◽

Cytochrome Oxidase ◽

Dna Barcoding ◽

Morphological Characters ◽

Species Level ◽

Coi Gene ◽

Interspecific Genetic Distance ◽

The Difference ◽

Source Of Information

Abstract Nudibranch has high species diversity with complex morphological characters and is challenging to identify at the species level. The lack of knowledge about nudibranchs makes it difficult to identify conventionally using morphological characters. This study aims to identify nudibranchs at the species level using the DNA barcoding method from the mitochondrial cytochrome oxidase 1 (CO1) gen. The results of DNA barcoding using the Cytochrome Oxidase I (COI) gene showed 18 species of 51 samples analyzed. The phylogenetic tree reconstruction revealed 11 main clades belonging to 11 genera. The genetic distance between and within species clearly shows the difference between individuals. Interspecific genetic distance shows the lowest value between species was found between Chromodoris annae and Chromodoris magnifica is 0.075, and the largest genetic distance observed between species Glossodoris rufomarginata and Tritonidae sp is 0.354. This study shows molecular analysis can be used to identify nudibranch up to species level, which will be a source of information in knowing the distribution and the genetic distance.

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External morphology and systematics of the family Lobalgidae (Acari: Astigmata) with resolution of the question of family monophyly

Insect Systematics & Evolution ◽

10.1163/187631210x496831 ◽

2010 ◽

Vol 41 (3) ◽

pp. 187-229 ◽

Cited By ~ 2

Author(s):

Barry OConnor ◽

Andre Bochkov

Keyword(s):

New Species ◽

Maximum Parsimony ◽

Taxonomic Revision ◽

Morphological Characters ◽

External Morphology ◽

Nomen Nudum ◽

The Family ◽

Two New Species

AbstractThe monophyly of the family Lobalgidae comprising the genera Lobalges, Echimytricalges and Coendalges is tested using morphological characters and the maximum parsimony approach. Representatives of all subfamilies of the family Psoroptidae, the families Pyroglyphidae (Paralgopsis) and Psoroptoididae (Psoroptoides), and most families of the superfamily Sarcoptoidea were used as close outgroups, with Ptiloxenus (Ptiloxenidae) and Acarus (Acaridae) selected as distant outgroups. The analysis revealed polyphyly of the family: the two lobalgid genera Lobalges and Echimytricalges appear as sister groups, whereas the genus Coendalges is a member of a clade comprising genera of the psoroptid subfamily Psoralginae. The composition of the family Lobalgidae is here limited to these two genera, and the genus Coendalges is placed in the subfamily Psoralginae (Psoroptidae). A taxonomic revision of the family Lobalgidae is given, including descriptions of two new species, Echimytricalges lonchothrix sp.n. and E. proechimys sp.n. We also validate the species Echimytricalges whitakeri Fain and Ritzi, which had previously been a nomen nudum.

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Revision of the Magellanic Mactridae Lamarck, 1809 (Bivalvia: Heterodonta)

Zootaxa ◽

10.11646/zootaxa.2757.1.4 ◽

2011 ◽

Vol 2757 (1) ◽

pp. 47 ◽

Cited By ~ 14

Author(s):

JAVIER H. SIGNORELLI ◽

GUIDO PASTORINO

Keyword(s):

Taxonomic Revision ◽

Morphological Characters ◽

Mantle Cavity ◽

Tierra Del Fuego ◽

Nominal Species ◽

Southwestern Atlantic ◽

Literature Research ◽

Southwestern Atlantic Ocean ◽

The Family ◽

Living Species

The worldwide family Mactridae Lamarck, 1809, is well represented in the southwestern Atlantic Ocean. Literature research revealed 22 living nominal species linked to Magellanic forms. Within this context, a taxonomic revision of the species belonging to the family Mactridae that inhabit the Magellanic area was carried out. The analysis was done by using morphological characters of the shell and describing the mantle cavity organs. After extensive fieldwork from Peninsula Valdés to Tierra del Fuego province, seven valid living species were confirmed. Synonymies of those valid forms have been updated, as have their geographic distributions. Redescriptions of the species here considered as valid, i.e.: Mactra fuegiensis Smith, 1905, Mulinia bicolor Gray, 1837, Mulinia byronensis Gray, 1837, Mulinia edulis (King & Broderip, 1832), Mulinia exalbida Gray, 1837, Mulinia levicardo (Smith 1881) and Darina solenoides (King & Broderip, 1832) are provided.

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Flourishing in subterranean ecosystems: Euro-Mediterranean Plusiocampinae and tachycampoids (Diplura, Campodeidae)

European Journal of Taxonomy ◽

10.5852/ejt.2020.591 ◽

2020 ◽

Cited By ~ 1

Author(s):

Alberto Sendra ◽

Dragan Antić ◽

Pablo Barranco ◽

Špela Borko ◽

Erhard Christian ◽

...

Keyword(s):

Organic Matter ◽

Mediterranean Region ◽

Body Shape ◽

Taxonomic Revision ◽

Terrestrial Ecosystems ◽

Mediterranean Area ◽

Sister Group ◽

Morphological Characters ◽

Incertae Sedis ◽

The Family

Diplura is a group of entognathous hexapods, often considered a sister group to insects. They play an important role in recycling organic matter in soil and subterranean terrestrial ecosystems. The Campodeidae is the most diverse family, divided into four subfamilies. The subfamily Plusiocampinae has a subterranean life-style with many species distributed in the Euro-Mediterranean area. The incertae sedis tachycampoids (“lignée Tachycampoïde”) is a group within the family Campodeidae that share with the Plusiocampinae a strong preference for subterranean habitats and several morphological characters, such as slender body shape, elongated appendages, considerable increment in the number of antennomeres and cercal articles, and complexity of sensorial structures. The present monograph provides a taxonomic revision of the subfamily Plusiocampinae and the genera belonging to the tachycampoid lineage from Europe and the Mediterranean region. It comprises detailed morphological descriptions and illustrations together with data on the habitats and distributions of 87 species, 10 subspecies and 11 affinis forms. Seven new species are described among those, namely: Plusiocampa (Plusiocampa) apollo Sendra, Giachino & Vailati sp. nov., P. (P.) chiosensis Sendra & Gasparo sp. nov., P. (P.) dublanskii Sendra & Turbanov sp. nov., P. (P.) hoffmanni Sendra & Paragamian sp. nov., P. (P.) rhea Sendra sp. nov., P. (P.) ternovensis Sendra & Borko sp. nov. and P. (Venetocampa) ferrani Sendra & Delić sp. nov.

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