scholarly journals Design and preliminary application of affinity peptide based on the structure of the porcine circovirus type II Capsid (PCV2 Cap)

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e8132 ◽  
Author(s):  
Junfang Hao ◽  
Fangyu Wang ◽  
Guangxu Xing ◽  
Yunchao Liu ◽  
Ruiguang Deng ◽  
...  
Keyword(s):  
Molecular Docking ◽  
Magnetic Beads ◽  
Ph Value ◽  
Porcine Circovirus Type ◽  
Single Step ◽  
Porcine Circovirus ◽  
Ligand Density ◽  
Affinity Adsorbent ◽  
Affinity Peptide ◽  
First Time

Background Affinity peptides, as a core part of affinity chromatography, play an important role in the purification of target molecules. Methods Here we describe the use of molecular docking technology for virtual screening of affinity peptides that specifically recognize the PCV2 Cap protein for the first time. Thirteen candidate peptides with high scores were obtained and then further characterized. Experimentally, the affinity and sensitivity of the peptides studied were identified by ELISA and LSPR, respectively. In order to investigate the purification effect of a selected peptide (L11) for the recombinant PCV2 Cap protein, it was coupled to NHS agarose magnetic beads as an affinity adsorbent (NaMB-L11); and the ligand density of the affinity adsorbent and pH value in the purification of the recombinant PCV2 Cap protein were optimized. Results Our data showed that the peptide L11- DYWWQSWE has the smallest KD = 103 nM with higher specificity for PCV2 Cap protein recognition. The NaMB-L11 affinity adsorbent yielded a purified Cap sample with 98% purity at 90% recovery in a single step. Conclusion Based on the structure, we obtained a high affinity peptide L11 binding to the PCV2 Cap protein by molecular docking technology. It not only provides a theoretical basis for the design of PCV2 Cap affinity peptide, but a new method for the purification of the PCV2 Cap protein.

scholarly journals The Nucleolar Localization Signal of Porcine Circovirus Type 4 Capsid Protein Is Essential for Interaction With Serine-48 Residue of Nucleolar Phosphoprotein Nucleophosmin-1

2021 ◽  
Vol 12 ◽  
Author(s):  
Jianwei Zhou ◽  
Yonghui Qiu ◽  
Ning Zhu ◽  
Linyi Zhou ◽  
Beining Dai ◽  
...  
Keyword(s):  
Porcine Circovirus Type ◽  
Cellular Proteins ◽  
Porcine Circovirus ◽  
Nucleolar Localization ◽  
Serine Residue ◽  
Nucleolar Localization Signal ◽  
Localization Signal ◽  
Charge Property ◽  
First Time ◽  
Oligomerization Domain

Porcine circovirus type 4 (PCV4) is an emerging etiological agent which was first detected in 2019. The nucleolar localization signal (NoLS) of PCV4 Cap protein and its binding host cellular proteins are still not elucidated. In the present study, we discovered a distinct novel NoLS of PCV4 Cap, which bound to the nucleolar phosphoprotein nucleophosmin-1 (NPM1). The NoLS of PCV4 Cap and serine-48 residue at the N-terminal oligomerization domain of NPM1 were necessary for PCV4 Cap/NPM1 interaction. Furthermore, the charge property of serine residue at position 48 of the NPM1 was crucial for its oligomerization and interaction with PCV4 Cap. In summary, our findings show for the first time that the PCV4 Cap NoLS and the NPM1 oligomerization determine the interaction of Cap/NPM1.

scholarly journals The serine-48 residue of nucleolar phosphoprotein nucleophosmin-1 plays critical role in subcellular localization and interaction with porcine circovirus type 3 capsid protein

2021 ◽  
Vol 52 (1) ◽  
Author(s):  
Jianwei Zhou ◽  
Juan Li ◽  
Haimin Li ◽  
Ying Zhang ◽  
Weiren Dong ◽  
...  
Keyword(s):  
Capsid Protein ◽  
Critical Role ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus ◽  
Nucleolar Localization ◽  
Nucleolar Localization Signal ◽  
Localization Signal ◽  
Charge Property ◽  
Type 3 ◽  
First Time

AbstractThe transport of circovirus capsid protein into nucleus is essential for viral replication in infected cell. However, the role of nucleolar shuttle proteins during porcine circovirus 3 capsid protein (PCV3 Cap) import is still not understood. Here, we report a previously unidentified nucleolar localization signal (NoLS) of PCV3 Cap, which hijacks the nucleolar phosphoprotein nucleophosmin-1 (NPM1) to facilitate nucleolar localization of PCV3 Cap. The NoLS of PCV3 Cap and serine-48 residue of N-terminal oligomerization domain of NPM1 are essential for PCV3 Cap/NPM1 interaction. In addition, charge property of serine-48 residue of NPM1 is critical for nucleolar localization and interaction with PCV3 Cap. Taken together, our findings demonstrate for the first time that NPM1 interacts with PCV3 Cap and is responsible for its nucleolar localization.

scholarly journals Swine viral detection by adapted Next-Generation Sequencing (NGS) for RNA and DNA species reveals first detection of porcine circovirus type 3 (PCV3) in Chile

2020 ◽  
Author(s):  
Paulina S. Rubilar ◽  
Javier Tognarelli ◽  
Jorge Fernández ◽  
Cristóbal Valdés ◽  
Felipe Broitman ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus ◽  
Next Generation ◽  
Animal Pathogens ◽  
Next Generation Sequencing Ngs ◽  
Type 3 ◽  
First Time ◽  
Generation Sequencing ◽  
Rna And Dna

AbstractThe outbreak and propagation of COVID-19 have posed a significant challenge to our society, highlighting the relevance of epidemiological monitoring of animal pathogens closely related to humans due to potential zoonosis. To find previously undetected viruses inside Chilean swine farms, we collected non-invasive samples from animals suspected to undergo any viral disease. After screening by Next Generation Sequencing (NGS) adapted to identify viral species with RNA and DNA genomes simultaneously, different viral species were successfully detected. Among viruses with an RNA genome, Porcine Rotavirus A (RVA), Porcine Astrovirus type 5 (PAstV-5) and Porcine Feces-Associated IASV-like virus (PfaIV) were identified; whereas among viruses with DNA genome, Porcine Parvovirus 1 (PPV1), Porcine Circovirus type 1 (PCV1) and Porcine Circovirus type 3 (PCV3) were found. PCV3, a recently described pathogenic virus, was detected for the first time in Chile in different samples from mummified tissue of stillbirths. The whole genome sequence of PCV3 was completed using conventional PCR and Sanger sequencing and compared with previously reported genomes. To the best of our knowledge, this is the first time that an adapted NGS system is used for the screening of DNA and RNA viral species simultaneously inside the Chilean swine industry. This adapted NGS system might be useful for the detection of swine viral emerging diseases worldwide.

scholarly journals Selection of affinity peptides for the purification potential of porcine circovirus type 2 (PCV2) Cap virus-like particles (VLPs)

RSC Advances ◽  
2017 ◽  
Vol 7 (62) ◽  
pp. 38911-38914 ◽  
Author(s):  
Guanmin Zheng ◽  
Qingxia Lu ◽  
Fangyu Wang ◽  
Qianyue Jin ◽  
Man Teng ◽  
...  
Keyword(s):  
Porcine Circovirus Type ◽  
Peptide Library ◽  
Porcine Circovirus ◽  
Random Peptide Library ◽  
Random Peptide ◽  
Peptide Motifs ◽  
M13 Phage ◽  
First Time ◽  
Selection Of

In this study, we describe the use of a M13 phage-displayed random peptide library for screening novel peptide motifs that specifically recognize recombinant PCV2 Cap protein for the first time.

scholarly journals In VitroIntragenomic Rearrangement of Porcine Circovirus Type 2

2012 ◽  
Vol 86 (16) ◽  
pp. 8911-8911 ◽  
Author(s):  
Libin Wen ◽  
Kongwang He ◽  
Yanxiu Ni ◽  
Xuehan Zhang ◽  
Bin Li ◽  
...  
Keyword(s):  
Molecular Evolution ◽  
Genome Sequence ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Porcine Circovirus ◽  
Associated Diseases ◽  
Pcv2 Strain ◽  
First Time ◽  
The Relationship

We report here for the first time the genome sequence of a rearranged porcine circovirus type 2 (PCV2) strain, CH-IVT1, isolated from PCV2-infected PK-15 cells. The complete circular genome of the CH-IVT1 is 605 nucleotides (nt) in length. The finding will help us to understand the molecular evolution of PCV2 and the relationship between PCV2 and PCV-associated diseases.

Immunoenhancement of Recombinant Neisseria meningitides PorB Protein on Porcine Circovirus Type 2 and Mycoplasma hyopneumoniae Genetically Engineered Vaccines

2019 ◽  
Vol 26 (10) ◽  
pp. 776-784
Author(s):  
Rui Yang ◽  
Yu Tao ◽  
Gaojian Li ◽  
Jian Chen ◽  
Jianhong Shu ◽  
...  
Keyword(s):  
Recombinant Protein ◽  
Mycoplasma Hyopneumoniae ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Genetically Engineered ◽  
Porcine Circovirus ◽  
Practical Applications ◽  
Neisseria Meningitides ◽  
Short Period

Background:Porcine circovirus and Mycoplasma hyopneumoniae can cause respiratory diseases in pigs, which cause serious economic loss in the worldwide pig industry. Currently, these infections are mainly prevented and controlled by vaccination. The new vaccines on the market are mainly composed of subunits and inactivated vaccines but usually have lower antigenicity than traditional live vaccines. Thus, there is an increasing need to develop new adjuvants that can cause rapid and long-lasting immunity to enhance the antigenic efficacy for vaccines. Studies have shown that meningococcal porin PorB can act as a ligand to combine with Toll-like receptors to activate the production of immunological projections and act as a vaccine immunological adjuvant.Objective:In this article, we expressed and purified the recombinant PorB protein and verified its immunogenicity against porcine circovirus type 2 and Mycoplasma hyopneumoniae genetically engineered vaccine.Methods:In this article, we used prokaryotic expression to express and purify recombinant PorB protein, four different concentrations of PorB protein, Freund's adjuvant with two genetically engineered vaccines were combined with subcutaneous immunization of mice.Results:Our study shows that the appropriate dose of the recombinant protein PorB can enhance the levels of humoral and cellular responses induced by two genetically engineered vaccines in a short period of time in mice. The PorB adjuvant group may cause statistically higher antibody titers for both genetically engineered vaccines compared to Freund's commercial adjuvant (P<0.001).Conclusion:The recombinant protein PorB may be a good candidate adjuvant for improving the protective effect of vaccines against porcine circovirus type 2 and Mycoplasma hyopneumoniae, and the protein can be used for future practical applications.

Effects of pH Value in the Electrolyte on the Properties of Cu2ZnSnS4Thin Film Fabricated by Single Step Co-Electrodeposition

2016 ◽  
Vol 5 (9) ◽  
pp. P521-P525 ◽  
Author(s):  
Hong Zhang ◽  
Shuying Cheng ◽  
Jinling Yu ◽  
Yunfeng Lai ◽  
Haifang Zhou ◽  
...  
Keyword(s):  
Ph Value ◽  
Single Step ◽  
Effects Of Ph

scholarly journals Broad scope gold(i)-catalysed polyenyne cyclisations for the formation of up to four carbon–carbon bonds

2017 ◽  
Vol 15 (10) ◽  
pp. 2163-2167 ◽  
Author(s):  
Zhouting Rong ◽  
Antonio M. Echavarren
Keyword(s):  
Single Step ◽  
Simultaneous Formation ◽  
Broad Scope ◽  
Carbon Carbon Bonds ◽  
Carbon Bonds ◽  
First Time ◽  
High Stereoselectivity

The polycyclisation of polyeneynes catalyzed by gold(i) has been extended for the first time to the simultaneous formation of up to four carbon–carbon bonds, leading to steroid-like molecules with high stereoselectivity in a single step with low catalyst loadings.

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