scholarly journals The combined effects of temperature and aromatase inhibitor on metamorphosis, growth, locomotion, and sex ratio of tiger frog (Hoplobatrachus rugulosus) tadpoles

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e8834
Author(s):  
Yun Tang ◽  
Zhi-Qiang Chen ◽  
You-Fu Lin ◽  
Jing-Yi Chen ◽  
Guo-Hua Ding ◽  
...  
Keyword(s):  
Body Size ◽  
Survival Rate ◽  
Sex Ratio ◽  
Aromatase Inhibitor ◽  
Condition Factor ◽  
Sex Ratios ◽  
Control Group ◽  
Treatment Groups ◽  
Effects Of Temperature ◽  
Steroid Hormone Level

Background The tiger frog (Hoplobatrachus rugulosus) is widely raised by many farms in southern region of China as an economically edible frog. The growth, development, and sexual differentiation of amphibians are influenced by temperature and steroid hormone level. However, the problem of hormone residues is caused by the addition of exogenous hormones in frog breeding, it is worth considering whether non-sterol aromatase inhibitors can be used instead of hormones. Methods In our study, H. rugulosus tadpoles were subjected to two water temperatures (29 °C and 34 °C) and three letrozole concentrations in the feed (0, 0.1 and 1 mg/g) to examine the effects of temperature, aromatase inhibitor and their interaction on metamorphosis, locomotion, and sex ratios. A G-test and contingency table were used to analyze the metamorphosis rate of tadpoles and the survival rate of froglets after feeding for 90 days. A G-test was also used to analyze sex ratios in different treatment groups. Results Metamorphosis time and body size (snout–vent length, body mass and condition factor) were significantly different between the two temperature treatments. Metamorphosis time was longer and body size was increased at 29 °C compared to those at 34 °C. Letrozole concentration and the temperature × letrozole interaction did not affect these variables. The jumping distance of froglets following metamorphosis was positively associated with the condition factor; when controlling for condition factor, jumping distance was not affected by temperature, letrozole concentration and their interaction. Temperature and letrozole concentration also did not affect metamorphosis and survival rate. Sex ratio of the control group (0 mg/g letrozole) was 1:1 at 29 °C, but there were more males at 34 °C. The sex ratios of H. rugulosus treated with letrozole at 29 °C and 34 °C were significantly biased toward males, and male ratio increased as letrozole concentration increased. Furthermore, more males were produced at 34 °C than at 29 °C at each letrozole concentration.

Immediate Loading of Unsplinted Implant Retained Mandibular Overdenture: A Randomized Controlled Clinical Study

2019 ◽  
Vol 45 (5) ◽  
pp. 378-389 ◽  
Author(s):  
Ahmad Kutkut ◽  
Mohamed Rezk ◽  
Dominique Zephyr ◽  
Dolphus Dawson ◽  
Robert Frazer ◽  
...  
Keyword(s):  
Clinical Study ◽  
Survival Rate ◽  
Test Group ◽  
Immediate Loading ◽  
Control Group ◽  
Gingival Index ◽  
Implant Survival ◽  
Implant Stability ◽  
Treatment Groups ◽  
Implant Survival Rate

This clinical study aimed to clinically and radiographically compare the implant survival rate and peri-implant tissue response between immediate and delayed loading protocols for unsplinted implant retained mandibular overdentures. Twenty patients were enrolled to participate in this study. Each subject was randomly assigned to 1 of 2 treatment groups: test group patients' implants (n = 10), which were immediately loaded, and control group patients' implants (n = 10), which were conventionally loaded. Locator abutments were torqued to 15 Ncm at delivery. Attachments were picked up intraorally immediately after implant placement for the test group and at 3 months for the control group, and 3-lb retention inserts were placed. Marginal bone levels based on cone beam computed tomography fixed reference points were recorded at baseline and 12 months. Modified plaque index, gingival index, and implant stability quotients were recorded at baseline, 3 months, and 12 months. After 12 months, implant survival rate was 100% in both groups. Marginal bone levels, keratinized mucosa, modified plaque index, and gingival index were significantly different among the groups at 3- and 12-month intervals, whereas no significant differences were found in implant stability quotients between the groups. The fact that implant survival rate was 100% in both treatment groups suggests that, within the limitations of this study, immediate loading protocol for unsplinted implant retained mandibular complete overdenture is as predictable, safe, and successful as the delayed loading protocol. Implementing the immediate loading protocol for mandibular implant retained overdentures could shorten treatment time, which could lead to better patient's satisfaction.

Effect of vitamin E on reproductive function in the mice treated with 2,3,7,8-tetrachlorodibenzo-p-dioxin*

2008 ◽  
Vol 24 (9) ◽  
pp. 595-601 ◽  
Author(s):  
JP Xu ◽  
YP Yin ◽  
XQ Zhou
Keyword(s):  
Body Weight ◽  
Vitamin E ◽  
Survival Rate ◽  
Sex Ratio ◽  
Pregnancy Rate ◽  
Reproductive Performance ◽  
Control Group ◽  
Group Iii ◽  
Group I ◽  
Experimental Group

The study was conducted to determine the effects of vitamin E on reproductive performance in mice treated with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). The 75 female and 40 male mice were randomly assigned into five groups. The levels of both TCDD and vitamin E given by gavage were 0 and 0 (Control group), 100 and 0 (experimental group I), 100 and 20 (experimental group II), 100 and 100 (experimental group III), and 100 ng/kg/day and 500 mg/kg/day (experimental group IV), respectively. Males and females were mixed to mate at the ratio of 1:2 after 4-week experiment. The gavage treatments were continued until the end of gestation in female mice after mating. The results showed that the litter number, survival rate, and body weight at birth of offspring in experimental group I had significantly decreased, and the females’ pregnancy rate and pup sex ratio in experimental group I had the decreasing tendencies when compared with the control group. The litter number in experimental group III, survival rate in experimental group II and III, body weight at birth in experimental group III and IV exhibited significant increase compared with experimental group I. The female pregnancy rate in both experimental group III and IV recovered to 100%, but there were no significant differences when compared with experimental group I. The pups’ sex ratio had a gradually increasing tendency with increase of vitamin E level, but there was no significant difference among experimental group I–IV. The results suggest that TCDD could induce reproductive toxicity in mice, whereas vitamin E alleviated adverse effects on reproductive performance in mice caused by TCDD.

scholarly journals EFEKTIVITAS PENAMBAHAN VITAMIN C (ASCORBIC ACID) PADA PAKAN KOMERSIAL UNTUK PENGENDALIAN PENYAKIT KOI HERPESVIRUS (KHV) PADA IKAN MAS, Cyprinus carpio

2016 ◽  
Vol 5 (3) ◽  
pp. 425
Author(s):  
Taukhid Taukhid ◽  
Angela Mariana Lusiastuti
Keyword(s):  
Survival Rate ◽  
Vitamin C ◽  
Clinical Symptoms ◽  
Control Group ◽  
Commercial Diet ◽  
Treatment Groups ◽  
Koi Herpesvirus ◽  
B Vitamin ◽  
Different Doses ◽  
All Treatment

Riset dengan tujuan untuk mengevaluasi teknik pengendalian penyakit KHV pada ikan mas melalui penggunaan imunostimulan yaitu penambahan vitamin C pada pakan komersial dengan dosis yang berbeda telah dilakukan pada skala laboratorium. Perlakuan yang diterapkan adalah penambahan vitamin C jenis CFC-90 (microencapsulated vitamin C) pada pakan komersial dengan berbagai dosis yaitu: (A) tanpa vitamin C, sebagai kontrol, (B) vitamin C sebanyak 250 mg/kg pakan, (C) vitamin C sebanyak 500 mg/kg pakan, (D) vitamin C sebanyak 750 mg/kg pakan, dan (E) vitamin C sebanyak 1.000 mg/kg pakan. Masing-masing perlakuan dilakukan pengulangan sebanyak 3 (tiga) kali. Pada hari ke-15, seluruh kelompok perlakuan diinfeksi KHV secara buatan dengan teknik kohabitasi. Pengamatan dilakukan terhadap tingkah laku, gejala klinis, dan mortalitas ikan uji yang dilakukan setiap hari hingga akhir percobaan. Rataan persen sintasan ikan uji tertinggi pada akhir riset diperoleh pada penambahan vitamin C sebanyak 750 mg/kg pakan yaitu sebesar 82,22%; diikuti dengan penambahan vitamin C 250 mg/kg pakan sebesar 70,00%; vitamin C 1.000 mg/kg pakan sebesar 61,11%; vitamin C 500 mg/kg pakan sebesar 58,89%; sedangkan rataan sintasan terendah diperoleh pada kelompok kontrol yaitu sebesar 27,78%.Research with the aim to evaluate the technique of KHV disease control carp through the use of immunostimulatory namely the addition of vitamin C in commercial diet with different doses have been performed in a laboratorial scale. The treatment applied was the addition of vitamin C of CFC-90 (microencapsulated vitamin C) in the commercial diet with different doses, namely: (A) without vitamin C, as control, (B) Vitamin C 250 mg/kg feed, (C) vitamin C of 500 mg/kg feed, (D) Vitamin C 750 mg/kg feed, and (E) of vitamin C as much as 1,000 mg/kg feed. Each treatment was repeated three times. On day 15th, all treatment groups were artificially infected with KHV using cohabitation techniques. Observations conducted to monitor behavior, clinical symptoms and mortality of fish tests were done every day until the end of the experiment. The highest mean percent of survival rate was recorded in treatment D by adding vitamin C as much as 750 mg/kg of feed that was equal to 82.22%, followed by the addition of vitamin C 250 mg/kg diets at 70.00% (treatment B), Vitamin C 1,000 mg/kg feed amounted to 61.11% (treatment E), vitamin C 500 mg/kg diets at 58.89% (treatment C), while the lowest average of survival rate was obtained in the control group that was equal to 27.78%.

scholarly journals Phenotypic and environmental predictors of reproductive success in painted turtles

2020 ◽  
Author(s):  
Jessica M. Judson ◽  
Luke A. Hoekstra ◽  
Kaitlyn G. Holden ◽  
Fredric J. Janzen
Keyword(s):  
Body Size ◽  
Reproductive Success ◽  
Mate Choice ◽  
Sex Ratio ◽  
Sex Ratios ◽  
Female Mate Choice ◽  
Painted Turtles ◽  
Weak Evidence ◽  
Adult Sex Ratio ◽  
Siring Success

ABSTRACTSexual selection is often assumed to elicit sexually dimorphic traits. However, most work on this assumption in tetrapod vertebrates has focused on birds. In this field experiment, we assessed relationships between both sexually dimorphic (body size, claw length) and non-dimorphic traits (forelimb stripe color, baseline corticosterone concentrations) and reproductive success in adult painted turtles to explicate the roles of these phenotypes in mate choice and the evolution of sexual dimorphism. We also modified adult sex ratios in experimental ponds to elucidate the role of biased sex ratios on reproductive success, which is a timely test of the potential threat of biased sex ratios on population persistence in a species with temperature-dependent sex determination. We found no strong influence of male phenotypes on male siring success, but female body size and baseline corticosterone concentrations predicted female clutch sizes. We find weak evidence that adult sex ratio influences male siring success, with a male-biased sex ratio producing lower male siring success than a female-biased sex ratio. This study offers evidence that female mate choice may not be an important selective force on male phenotypes, but that instead selection occurs on female phenotypes, particularly body size and corticosterone concentrations. Further, biased adult sex ratios can influence reproductive success of both sexes. Finally, the use of Kompetitive Allele Specific PCR (KASP) was highly successful in parentage analysis, which adds reptiles to the growing list of taxa successfully genotyped with this new technology.Lay SummaryFemale painted turtles aren’t choosy about traits of their mates. In a field experiment, we find that male traits do not predict male fitness, but key female traits (body size and stress levels) do predict female reproductive success. Further, we find weak evidence that adult sex ratio influences individual fitness in this species with environmental sex determination. Ultimately, we reject the long-assumed importance of female mate choice in this freshwater turtle.

Unexpected offspring sex ratios in response to habitat quality in a size-dimorphic bark beetle

1999 ◽  
Vol 77 (4) ◽  
pp. 524-529 ◽  
Author(s):  
Risa D Sargent ◽  
Mary L Reid
Keyword(s):  
Body Size ◽  
Reproductive Success ◽  
Sex Ratio ◽  
Habitat Quality ◽  
Bark Beetle ◽  
Sex Ratios ◽  
Diploid Species ◽  
Differential Mortality ◽  
Male Reproductive Success ◽  
Offspring Sex

Facultative sex ratio manipulation has been examined in a limited number of diploid species, mainly vertebrates. We tested the prediction that mothers would preferentially place males in conditions conducive to large size in the diploid pine engraver bark beetle, Ips pini. In this species, males are the larger sex and therefore male reproductive success was expected to be more dependent on body size than female reproductive success. Because body size is largely environmentally determined in bark beetles, mothers were expected to alter sex ratios in response to habitat quality. Contrary to predictions, offspring sex ratios tended to be more female biased in situations conducive to large offspring size than in situations producing offspring of small size. We were able to rule out nonadaptive explanations such as differential mortality or development times of males and females, suggesting that the observed pattern is adaptive. This study provides a rare example of sex ratio manipulation in diploid insects. However, the unexpected direction of sex ratio biases suggests that daughters gain a yet unknown benefit from being reared in high-quality conditions that surpasses the fitness that would be gained from producing relatively larger sons.

163 IN VITRO CULTURE OF PORCINE OOCYTE-GRANULOSA CELL COMPLEXES

2011 ◽  
Vol 23 (1) ◽  
pp. 184
Author(s):  
Y. F. Diao ◽  
R. X. Han ◽  
H. R. Kim ◽  
C. S. Park ◽  
D. I. Jin
Keyword(s):  
Survival Rate ◽  
Granulosa Cell ◽  
Granulosa Cells ◽  
Control Group ◽  
Oocyte Diameter ◽  
Cell Complexes ◽  
Treatment Groups ◽  
Significant Difference ◽  
Porcine Oocyte

The objective of this study was to investigate the effects of porcine follicular fluid (PFF) and insulin-like growth factor-1 (IGF-1) on the growth of porcine oocyte-granulosa cell complexes (OGC) in vitro, in an effort to improve meiotic and developmental competence in vitro. Porcine OGC were manually dissected from early antral follicles of diameter 400 to 700 μm, and intact oocytes with undamaged granulosa cells were selected for culture. Between 3 and 5 OGC were combined in 50-uL droplets and cultured for 12 days in M199 medium supplemented with PVP, oestradiol, FSH, transferrin, L-ascorbic acid, and insulin. The OGC were cultured at 38.5°C in a humidified atmosphere of 5% CO2 in air for 12 days, and the oocytes were matured for 44 h. Oocyte diameter, exclusive of the zona pellucida, was measured on day 0 and day 12 of culture. Control group was cultured in the absence of PFF or IGF-1. The experiment was divided into 2 parts. In part 1, treatment groups were cultured with 2.5, 5.0, or 7.5% (all v/v) PFF. Control OGC grew as spheres that were formed by granulosa cells. In treatment groups, the granulosa cells spread and grew on the bottom of dishes. When oocyte diameter was measured after 12 days of culture, no significant difference among groups was observed (104.07, 103.96, and 104.27 μm at the 3 PFF concentrations used; control: 104.03 μm). Similarly, the survival rate of oocytes did not differ significantly among groups. However, survival rate fell somewhat in the group treated with PFF (control: 65%; tests: 58.87, 58.33, and 50.83% at the 3 PFF concentrations used). The maturation rates of oocytes in the control was significantly higher than those of the treatment groups [25.83% (control) v. 14, 11.67, and 3.67% (the 3 treatment groups); P < 0.05]. Thus, the first conclusion is that supplementation of culture medium with PFF did not enhance the development of porcine OGC in vitro. In part 2 of the experiment, treatment groups were cultured with 10, 50, or 100 ng mL–1 IGF-1. The percentages of OGC showing antrum formation were 80, 80, 100, and 100% in groups treated with 0, 10, 50, or 100 ng mL–1 IGF-1, respectively. Average oocyte diameter was 94.16 to 94.58 μm just after OGC collection. However, the average diameter of oocytes cultured for 12 days with 50 or 100 ng mL–1 IGF-1 was significantly higher than that of the control or 10 ng mL–1 groups [108.88 and 108.31 μm (50 and 100 ng mL–1 IGF-1) v. 105.98 μm and 106.67 μm (0 and 10 ng mL–1 IGF-1); P < 0.05]. The maturation rate of oocytes grown with 10 and 50 ng mL–1 IGF-1 was higher than those of the other 2 groups [30 and 40.6% (10 and 50 ng mL–1 IGF-1, respectively) v. 25 and 26% (0 and 100 ng mL–1 IGF-1, respectively); P < 0.05]. Thus, the second conclusion is that 50 ng mL–1 IGF-1 improves the growth and maturation of porcine oocyte-granulosa cell complexes in vitro.

Sex ratio and body size among hatchlings of habu, Trimeresurus flavoviridis, from the Okinawa Islands, Japan

1993 ◽  
Vol 14 (3) ◽  
pp. 275-283 ◽  
Author(s):  
Takao Kamura ◽  
Masahiko Nishimura
Keyword(s):  
Body Size ◽  
Sex Ratio ◽  
Sex Ratios ◽  
Maternal Size ◽  
Trimeresurus Flavoviridis

AbstractEmbryo and hatchling sex ratios were unbiased in samples of habu, Trimeresurus flavoviridis, from Okinawa, Japan. Sex ratios dit not vary with maternal size or age. Large eggs produced large hatchlings with high proportions of residual yolk. Slender eggs produced lighter hatchlings.

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