scholarly journals Genetic characterization of feline panleukopenia virus from dogs in Vietnam reveals a unique Thr101 mutation in VP2

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e9752
Author(s):  
Minh Hoang ◽  
Cheng-Nan Wu ◽  
Chuen-Fu Lin ◽  
Huong Thanh Thi Nguyen ◽  
Van Phan Le ◽  
...  
Keyword(s):  
Phylogenetic Analyses ◽  
Molecular Surface ◽  
Contact Potential ◽  
Vp2 Gene ◽  
Feline Panleukopenia Virus ◽  
Feline Parvovirus ◽  
Canine Parvovirus Type 2

Background Canine parvovirus type 2 (CPV-2) and feline parvovirus (FPV) are known as the main causes of several serious diseases and have a severe impact on puppies and kittens, respectively. FPV and new CPV-2 variants are all able to infect cats, causing diseases indistinguishable from feline panleukopenia. However, FPV only replicates efficiently in feline cells in vitro and replicates in dogs in the thymus and bone marrow without being shed in feces. In our previous study, the genotypes of six parvoviral isolates were unable to be identified using a SimpleProbe® real-time PCR assay. Methods In the present study, we characterized previously unidentified FPV-like viruses isolated from dogs in Vietnam. The six isolates were utilized to complete VP2 gene sequencing and to conduct phylogenetic analyses. Results Sequence analysis of the six parvoviral strains identified the species as being similar to FPV. Phylogenetic analysis demonstrated that the complete VP2 genes of the strains are similar to those of FPV. The FPV-like strains contain a Thr101 mutation in the VP2 protein, which is different from prototype FPV strains. Discussion Our data provide evidence for the existence of changes in the charge, protein contact potential and molecular surface of the core of the receptor-binding size with an Ile101 to Thr101 mutation. This is also the first study to provide reliable evidence that FPV may be a threat to the Vietnamese dog population.

MOLECULAR EPIDEMIOLOGY OFCANINE PARVOVIRUS TYPE2A AND 2B AMONG SHELTER DOGS — THE NEW RECOMBINATION IN VIRUS

2017 ◽  
Vol 43 (01) ◽  
pp. 47-53
Author(s):  
Ming-Hua Sung ◽  
Tsung-Ming Chen ◽  
Yi-Chen Yang ◽  
Yuan-Ting Lu ◽  
Man-Yu Hsu ◽  
...  
Keyword(s):  
Viral Evolution ◽  
Pcr Amplification ◽  
Vp2 Gene ◽  
Blood Samples ◽  
Maximum Likelihood Phylogenetic Tree ◽  
Shelter Dogs ◽  
Central Taiwan ◽  
Canine Parvovirus Type 2

Canine parvovirus type 2 (CPV-2) is a severe enteric pathogen mainly affecting dogs. CPV-2 contains three antigenic variants (2a, 2b, and 2c) that are distributed internationally. Detection and characterization of the currently circulating CPV-2 strains are vital for the understanding of viral evolution, transmission, and the development of methods to control its spreading. Herein, we analyzed the strains in central Taiwan to provide information of local viral evolution, diversity, and epidemiology. Stool and blood samples from 24 dogs and 2 cats were genotyped by PCR amplification of strain-specific VP2 sequence collected during 2011–2013. 60% (16/26) of them were positive and 100% (16/16) of these positive samples were type 2b. Then, the full length VP2 gene was sequenced in 6 CPV-positive samples and a maximum-likelihood phylogenetic tree was constructed using both Taiwan and other strains worldwide. Surprisingly, all Taiwan CPVs showed high relatedness to type 2a. Recombination analysis revealed a recombination of VP2 gene between type 2a and 2b. This study demonstrates a recombination between CPV-2a and 2b in nature that contributes to the genetic diversity and evolution of CPV-2.

scholarly journals Molecular Characteristics and Genetic Evolutionary Analyses of Circulating Parvoviruses Derived From Cats in Beijing

2021 ◽  
Author(s):  
Yashu Tang ◽  
Na Tang ◽  
Jingru Zhu ◽  
Yang Liu ◽  
Yanli Lyu
Keyword(s):  
Molecular Characteristics ◽  
Vp2 Gene ◽  
Fecal Samples ◽  
Evolutionary Pattern ◽  
Specific Pcr ◽  
Genetic Features ◽  
Feline Parvovirus ◽  
Beijing China ◽  
Canine Parvovirus Type 2

Abstract Background: Feline parvovirus (FPV) is a member of the Parvoviridae family, which is a major enteric pathogen of cats worldwide. This study aimed to investigate the prevalence of feline parvovirus in Beijing, China and analyze the genetic features of detected viruses.Results: In this study, a total of 60 parvovirus-positive samples (8.5%) were detected from 702 cat fecal samples using parvovirus specific PCR. The complete VP2 genes were amplified from all these samples. Among them, 55 sequences were characterized as FPV (91.7%), the other five were typed as canine parvovirus type 2 (CPV-2) variants (8.3%), comprised of four CPV-2c and a new CPV-2b strain. In order to identify the origin of CPV-2 variants in cats, we amplified full-length VP2 genes from 7 fecal samples of dogs infected with CPV-2, which were further classified as CPV-2c. Moreover, the sequences of new CPV-2b/MT270586 and CPV-2c/MT270587 detected from feline samples shared 100% identity with previous canine isolates KT156833 and MF467242 respectively, indicating the CPV-2 variants circulating in cats were derived from dogs. Sequence analysis indicated new mutations, Ala91Ser and Ser192Phe, in the FPV sequences, while obtained CPV-2c carried mutations reported in Asian CPV variants, showing they share a common evolutionary pattern with the Asian 2c strains. Interestingly, the FPV sequence (MT270571), displaying four CPV-specific residues, was found to be a putative recombinant sequence between CPV-2c and FPV. Phylogenetic analysis of the VP2 gene showed that amino acid and nucleotide mutations promoted the evolution of different geographical branches of FPV and CPV lineages.Conclusions: Our findings will be helpful to further understand the circulation and evolution of canine and feline parvovirus in Beijing.

scholarly journals Phylogenetic analysis of canine parvoviruses from Turkey

2020 ◽  
Vol 76 (01) ◽  
pp. 6334-2020
Author(s):  
ZEYNEP AKKUTAY-YOLDAR ◽  
TAYLAN KOÇ B.
Keyword(s):  
High Mortality ◽  
Phylogenetic Trees ◽  
Clinical Symptoms ◽  
Phylogenetic Analyses ◽  
Canine Parvovirus ◽  
Mortality And Morbidity ◽  
Identity Match ◽  
Genomic Characterization

Canine parvovirus (CPV) type 2 is the causative agent of acute hemorrhagic enteritis and high mortality in the affected dogs. Numerous studies have been done to understand the origin of the virus and to exhibit new variants and circulating strains. This report describes the detection and genomic characterization of CPV strains from indoor and outdoor dogs in Ankara, Turkey. Samples were sent to our laboratory due to clinical symptoms in puppies. We tested blood and swab samples to determine the presence of canine parvovirus (CPV) in three puppies and two adult dogs by reverse transcription-polymerase chain reaction (RT-PCR) using VP2 (capsid protein) region primers of canine parvoviruses. Following that, to provide molecular characterization data Maximum Likelihood (ML) method was used for phylogenetic analyses. Constructed phylogenetic trees from the aligned nucleotide sequences revealed that our CPV strains demonstrated high genetic similarities, with 100% identity match on nucleotide alignments with each other and classified in CPV-2b genotypes.They have placed on a monophyletic clade as a sister branch with CPV VAC S quantum with 98.9% nucleotide homology. Our findings suggest that CPV-2b is actual and frequently seen variant in Turkey and shows high similarities with other CPV variants and a bit less with FPVs in Turkey and around the world. CPV causes high mortality and morbidity in dogs and to develop effective vaccines for protection of dogs in Turkey where there are few numbers of studies that have been done, field strains should be isolated and characterised.

scholarly journals A phylogenetic study of canine parvovirus type 2c in midwestern Brazil

2013 ◽  
Vol 33 (2) ◽  
pp. 214-218 ◽  
Author(s):  
Danúbia S. Fontana ◽  
Paulo Ricardo D. Rocha ◽  
Raquel A.S. Cruz ◽  
Letícya L. Lopes ◽  
Andréia L.T. Melo ◽  
...  
Keyword(s):  
Amino Acid ◽  
Genetic Variability ◽  
Dna Sequencing ◽  
Canine Parvovirus ◽  
Phylogenetic Study ◽  
Vp2 Gene ◽  
Hemorrhagic Enteritis ◽  
Canine Parvovirus Type 2 ◽  
Representative Samples

Since the late 1970s, canine parvovirus type 2 (CPV-2) has emerged as a causative agent of fatal severe acute hemorrhagic enteritis in dogs. To date, three antigenic types of CPV-2 were described worldwide (CPV-2a/b/c). This study was conducted to determine the variants of CPV-2 circulating in dogs from the Cuiabá Municipality in Midwestern Brazil. Out of 50 fecal samples, collected between 2009 and 2011, 27 tested positive for CPV-2. A 583 bp fragment of the VP2 gene was amplified by PCR, 13 representative samples were analyzed further by DNA sequencing. All strains were characterized as CPV-2c, displayed a low genetic variability although observed several amino acid substitution. These findings indicated that CPV-2c has been circulating in dogs from the Cuiabá Municipality in Midwestern Brazil.

Genetic characterization of canine parvovirus type 2 subtypes in Maputo, Mozambique

2016 ◽  
Vol 199 (4) ◽  
pp. 543-549 ◽  
Author(s):  
J. Figueiredo ◽  
C. Miranda ◽  
R. Souto ◽  
E. Silva ◽  
J. Fafetine ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
Canine Parvovirus ◽  
Canine Parvovirus Type 2

scholarly journals The increasing prevalence of CPV-2c in domestic dogs in China

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e9869 ◽  
Author(s):  
Xiangqi Hao ◽  
Yuwei He ◽  
Chuhan Wang ◽  
Weiqi Xiao ◽  
Ruohan Liu ◽  
...  
Keyword(s):  
Gene Sequence ◽  
Virus Isolation ◽  
Acid Sites ◽  
High Morbidity ◽  
Vp2 Gene ◽  
Carnivore Species ◽  
Nucleotide Database ◽  
Canine Parvovirus Type 2 ◽  
Multiple Variants

Background Canine parvovirus type 2 (CPV-2), a serious pathogen, leads to high morbidity and mortality in dogs and several wild carnivore species. Although it is a DNA virus, it evolves particularly rapidly, with a genomic substitution rate of approximately 10−4 substitutions/site/year, close to that of some RNA viruses. Tracing the prevalence of CPV-2 in dogs is significant. Methods In this study, an aetiological survey was carried out from 2016 to 2019 in Guangdong Province, China, involving Guangzhou, Shenzhen and Dongguan. Furthermore, to systematically analyse the prevalence of CPV-2 in China, the VP2 gene sequences of all Chinese isolates were downloaded from the NCBI nucleotide database in December 2019, and changes in CPV-2 variants were examined. Results A total of 55.7% (34/61) of samples were CPV-2 positive by PCR detection and virus isolation. In addition to different variants circulating in dogs, coinfection with multiple variants was identified, as was coinfection with other canine enteric pathogens in some cases. Two previously reported amino acid sites, A5G and Q370R of CPV-2c mutants, reported in variants in China were assessed, and several CPV-2 isolates with P13S and K582N mutations were detected in this study. Finally, we speculate on the prevalence of different CPV-2 variants in China. According to the VP2 gene sequence obtained from the NCBI nucleotide database, the proportion of different variants in China has changed, and CPV-2c appears to be growing rapidly. In conclusion, this aetiology survey suggests that CPV-2 continues to be common in China and that the prevalence of CPV-2c is increasing.

scholarly journals Feline panleukopenia virus revisited : molecular characteristics and pathological lesions associated with three recent isolates

2000 ◽  
Vol 71 (3) ◽  
Author(s):  
M. Van Vuuren ◽  
A. Steinel ◽  
T. Goosen ◽  
E. Lane ◽  
J. Van der Lugt ◽  
...  
Keyword(s):  
Clinical Signs ◽  
Domestic Cat ◽  
Molecular Characteristics ◽  
Chain Reaction ◽  
Feline Panleukopenia Virus ◽  
Pathological Lesions ◽  
Polymerase Chain ◽  
Low Incidence ◽  
Canine Parvovirus Type 2

The low incidence of clinical signs or pathological lesions compatible with feline panleukopenia in cats has created the perception among practitioners that the disease has disappeared since the emergence of canine parvovirus type 2 in the late 1970s.Three parvoviruses that were recently isolated from a domestic cat and 2 cheetahs in cell culture or detected by means of the polymerase chain reaction were shown to be typical feline parvoviruses. Phylogenetic comparison with other FPV isolates did not reveal a particular African cluster.

scholarly journals Characterization of Resveratrol, Oxyresveratrol, Piceatannol and Roflumilast as Modulators of Phosphodiesterase Activity. Study of Yeast Lifespan

2020 ◽  
Vol 13 (9) ◽  
pp. 225
Author(s):  
Adrián Matencio ◽  
Francisco García-Carmona ◽  
José Manuel López-Nicolás
Keyword(s):  
Pde4 Inhibitor ◽  
Chronic Obstructive ◽  
Phosphodiesterase Activity ◽  
Chronic Obstructive Pulmonary Diseases ◽  
Phosphodiesterase Type ◽  
Approved Drugs

Our desire to live longer has led to an ever-increasing number of novel antiaging products. However, few molecules have any real effect and new ones need to be studied before they can be used commercially. In this contribution, activation of the caloric restriction (CR) pathway was studied using different three (resveratrol, oxyresveratrol and piceatannol)—a family with demonstrated bioactivity on phosphodiesterase activity. The high-affinity phosphodiesterase type 2 (PDE2) of Saccharomyces cerevisiae was expressed in Escherichia coli, purified and characterized. The activity and the inhibitory activity of each stilbene was studied, and the findings were compared in vitro and in silico with those obtained with roflumilast—a human PDE4 inhibitor widely used in chronic obstructive pulmonary diseases. Finally, an in vivo chronological lifespan assay using WT S. cerevisiae and ΔPDE2 S. cerevisiae strains was carried out. It was demonstrated that stilbenes can modulate yPDE2 activity, increasing the lifespan of the yeast by 18% over a control (in combination with other pathways). In addition, roflumilast increased the lifespan in the WT strain. The findings as a whole would increase the range of lifespan products available and suggest novel uses for approved drugs.

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