The Use of Circulating miRNAs as Biomarkers for Oxidative Stress in Critically Ill Polytrauma Patients

2016 ◽  
Vol 62 (03/2016) ◽  
Author(s):  
Ovidiu Bedreag ◽  
Dorel Sandesc ◽  
Sorin Chiriac ◽  
Alexandru Rogobete ◽  
Alina Cradigati ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Critically Ill ◽  
Circulating Mirnas ◽  
Polytrauma Patients ◽  
Biomarkers For Oxidative Stress

scholarly journals Analysis of oxidative stress-related markers in critically ill polytrauma patients: An observational prospective single-center study

2018 ◽  
Vol 18 (2) ◽  
pp. 191-197 ◽  
Author(s):  
Mihai Sandesc ◽  
Alexandru Florin Rogobete ◽  
Ovidiu Horea Bedreag ◽  
Anca Dinu ◽  
Marius Papurica ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Critically Ill ◽  
Antioxidant Therapy ◽  
Sodium Ascorbate ◽  
High Density Lipoproteins ◽  
Apache Ii Score ◽  
Control Group ◽  
Antioxidant Treatment ◽  
Incidence And Mortality ◽  
Polytrauma Patients

Critically ill polytrauma patients have increased production of free radicals (FRs) and consequent alterations in biochemical pathways, as well as disruption of cellular integrity, due to increased lipid peroxidation. The aim of this study was to investigate several biomarkers associated with increased oxidative stress in critically ill polytrauma patients, and to evaluate the effect of antioxidant treatment on the clinical outcome in these patients. A total of 67 polytrauma patients from an intensive care unit met the selection criteria. Antiox group included 35/67 patients who received antioxidant therapy, while 32/67 patients without antioxidant treatment were considered as control group. Antioxidant therapy consisted of simultaneous administration of Vitamin C (sodium ascorbate) and N-acetylcysteine, through continuous intravenous infusion. Clinical and paraclinical evaluation of the patients was performed daily until discharge or death. At admission, laboratory parameters did not differ significantly between two groups. At discharge/upon death, statistically significant differences in favor of Antiox group were observed in the following parameters: thrombocytes, activated partial thromboplastin time, prothrombin time, total bilirubin, total cholesterol, high-density lipoproteins, low-density lipoproteins, erythrocyte sedimentation rate, interleukin 6 (all p = 0.0001), total protein (p = 0.0005), serum albumin (p = 0.0004), lactate dehydrogenase (p = 0.0006), and C-reactive protein (p = 0.0014). Starting from day 5, the APACHE II score was significantly decreased in Antiox versus control group (p < 0.05). Finally, the sepsis incidence and mortality rate were significantly lower in Antiox group (p < 0.05). Decreasing the level of oxidative stress by antioxidant substances significantly correlated with a better prognosis and outcome in our patients. Further studies should elucidate more clearly the mechanism of action of antioxidants in critically ill polytrauma patients.

Using Circulating miRNAs as Biomarkers for the Evaluation and Monitoring of the Mitochondrial Damage in the Critically Ill Polytrauma Patients

2016 ◽  
Vol 62 (08/2016) ◽  
Author(s):  
Ovidiu Bedreag ◽  
Marius Papurica ◽  
Alexandru Rogobete ◽  
Dorel Sandesc ◽  
Raluca Dumache ◽  
...  
Keyword(s):  
Critically Ill ◽  
Mitochondrial Damage ◽  
Circulating Mirnas ◽  
Polytrauma Patients ◽  
Evaluation And Monitoring

scholarly journals Characterization of Differentially Expressed Circulating miRNAs in Metabolically Healthy versus Unhealthy Obesity

Biomedicines ◽  
2021 ◽  
Vol 9 (3) ◽  
pp. 321
Author(s):  
Susana Rovira-Llopis ◽  
Rubén Díaz-Rúa ◽  
Carmen Grau-del Valle ◽  
Francesca Iannantuoni ◽  
Zaida Abad-Jimenez ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Metabolism ◽  
Insulin Signalling ◽  
Cross Sectional Study ◽  
Differentially Expressed ◽  
Circulating Mirnas ◽  
Oxidative Stress Biomarkers ◽  
Cross Sectional ◽  
Metabolically Healthy Obese ◽  
Metabolically Healthy

Obese individuals without metabolic comorbidities are categorized as metabolically healthy obese (MHO). MicroRNAs (miRNAs) may be implicated in MHO. This cross-sectional study explores the link between circulating miRNAs and the main components of metabolic syndrome (MetS) in the context of obesity. We also examine oxidative stress biomarkers in MHO vs. metabolically unhealthy obesity (MUO). We analysed 3536 serum miRNAs in 20 middle-aged obese individuals: 10 MHO and 10 MUO. A total of 159 miRNAs were differentially expressed, of which, 72 miRNAs (45.2%) were higher and 87 miRNAs (54.7%) were lower in the MUO group. In addition, miRNAs related to insulin signalling and lipid metabolism pathways were upregulated in the MUO group. Among these miRNAs, hsa-miR-6796-5p and hsa-miR-4697-3p, which regulate oxidative stress, showed significant correlations with glucose, triglycerides, HbA1c and HDLc. Our results provide evidence of a pattern of differentially expressed miRNAs in obesity according to MetS, and identify those related to insulin resistance and lipid metabolism pathways.

scholarly journals Real‐Time Energy Exposure Is Associated With Increased Oxidative Stress Among Feeding‐Tolerant Critically Ill Patients: Results From the FEDOX Trial

2020 ◽  
Vol 44 (8) ◽  
pp. 1484-1491 ◽  
Author(s):  
Liam McKeever ◽  
Sarah J. Peterson ◽  
Sofia Cienfuegos ◽  
Jaime Rizzie ◽  
Omar Lateef ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Real Time ◽  
Critically Ill ◽  
Critically Ill Patients

Discovery of biomarkers for oxidative stress based on cellular metabolomics

Biomarkers ◽  
2016 ◽  
Vol 21 (5) ◽  
pp. 449-457 ◽  
Author(s):  
Ningli Wang ◽  
Jianteng Wei ◽  
Yewei Liu ◽  
Dong Pei ◽  
Qingping Hu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Biomarkers For Oxidative Stress ◽  
Cellular Metabolomics

Oxidative Stress in Critically Ill Patients

2002 ◽  
Vol 11 (6) ◽  
pp. 543-551 ◽  
Author(s):  
Caryl Goodyear-Bruch ◽  
Janet D. Pierce
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Superoxide Dismutase ◽  
Free Radicals ◽  
Critically Ill ◽  
Critically Ill Patients ◽  
Oxygen Free Radicals ◽  
Oxygen Species ◽  
Defense System ◽  
Oxygen Derived Free Radicals

Oxygen-derived free radicals play an important role in the development of disease in critically ill patients. Normally, oxygen free radicals are neutralized by antioxidants such as vitamin E or enzymes such as superoxide dismutase. However, in patients who require intensive care, oxygen free radicals become a problem when either a decrease in the removal or an overproduction of the radicals occurs. This oxidative stress and the damage due to it have been implicated in many diseases in critically ill patients. Many drugs and treatments now being investigated are directed toward preventing the damage from oxidative stress. The formation of reactive oxygen species, the damage caused by them, and the body’s defense system against them are reviewed. New interventions are described that may be used in critically ill patients to prevent or treat oxidative damage.

Serum Coenzyme Q10 Levels are Decreased in Critically-Ill Septic Patients: Results From a Preliminary Study

2020 ◽  
pp. 109980042094448
Author(s):  
Alice G. Vassiliou ◽  
Zafeiria Mastora ◽  
Edison Jahaj ◽  
Chrysi Keskinidou ◽  
Maria E. Pratikaki ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Septic Shock ◽  
Critically Ill ◽  
Coenzyme Q10 ◽  
Inflammatory Biomarkers ◽  
Healthy Controls ◽  
Icu Patients ◽  
Icu Admission ◽  
Icu Discharge ◽  
Sepsis And Septic Shock

Background: The increased oxidative stress resulting from the inflammatory responses in sepsis initiates changes in mitochondrial function which may result in organ damage, the most common cause of death in the intensive care unit (ICU). Deficiency of coenzyme Q10 (CoQ10), a key cofactor in the mitochondrial respiratory chain, could potentially disturb mitochondrial bioenergetics and oxidative stress, and may serve as a biomarker of mitochondrial dysfunction. Hence, we aimed to investigate in initially non-septic patients whether CoQ10 levels are decreased in sepsis and septic shock compared to ICU admission, and to evaluate its associations with severity scores, inflammatory biomarkers, and ICU outcomes. Methods: Observational retrospective analysis on 86 mechanically-ventilated, initially non-septic, ICU patients. CoQ10 was sequentially measured on ICU admission, sepsis, septic shock or at ICU discharge. CoQ10 was additionally measured in 25 healthy controls. Inflammatory biomarkers were determined at baseline and sepsis. Results: On admission, ICU patients who developed sepsis had lower CoQ10 levels compared to healthy controls (0.89 vs. 1.04 µg/ml, p < 0.05), while at sepsis and septic shock CoQ10 levels decreased further (0.63 µg/ml; p < 0.001 and 0.42 µg/ml; p < 0.0001, respectively, from admission). In ICU patients who did not develop sepsis, admission CoQ10 levels were also lower than healthy subjects (0.81 µg/ml; p < 0.001) and were maintained at the same levels until discharge. Conclusion: CoQ10 levels in critically-ill patients are low on ICU admission compared to healthy controls and exhibit a further decrease in sepsis and septic shock. These results suggest that sepsis severity leads to CoQ10 depletion.

scholarly journals Oxidative Stress and Antioxidant Therapy in Critically Ill Polytrauma Patients with Severe Head Injury

2015 ◽  
Vol 1 (3) ◽  
pp. 83-91 ◽  
Author(s):  
Loredana Luca ◽  
Alexandru Florin Rogobete ◽  
Ovidiu Horea Bedreag
Keyword(s):  
Oxidative Stress ◽  
Critically Ill ◽  
Clinical Status ◽  
Antioxidant Therapy ◽  
Pathophysiological Mechanism ◽  
Microvascular Damage ◽  
Traumatic Injuries ◽  
Blood Brain Barrier Disruption ◽  
Brain Barrier Disruption ◽  
Blood Spinal Cord Barrier

Abstract Traumatic Brain Injury (TBI) is one of the leading causes of death among critically ill patients from the Intensive Care Units (ICU). After primary traumatic injuries, secondary complications occur, which are responsible for the progressive degradation of the clinical status in this type of patients. These include severe inflammation, biochemical and physiological imbalances and disruption of the cellular functionality. The redox cellular potential is determined by the oxidant/antioxidant ratio. Redox potential is disturbed in case of TBI leading to oxidative stress (OS). A series of agression factors that accumulate after primary traumatic injuries lead to secondary lesions represented by brain ischemia and hypoxia, inflammatory and metabolic factors, coagulopathy, microvascular damage, neurotransmitter accumulation, blood-brain barrier disruption, excitotoxic damage, blood-spinal cord barrier damage, and mitochondrial dysfunctions. A cascade of pathophysiological events lead to accelerated production of free radicals (FR) that further sustain the OS. To minimize the OS and restore normal oxidant/antioxidant ratio, a series of antioxidant substances is recommended to be administrated (vitamin C, vitamin E, resveratrol, N-acetylcysteine). In this paper we present the biochemical and pathophysiological mechanism of action of FR in patients with TBI and the antioxidant therapy available.

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