scholarly journals Brugada-like ECG Pattern in COVID-19 Infection

2020 ◽  
Author(s):  
Amir Tavoosi ◽  
Mehrangiz Zangeneh ◽  
Mansoor Mohsen Abadi ◽  
Mahin Nomali ◽  
Kian Alipasandi
Keyword(s):  
Hospital Stay ◽  
Troponin I ◽  
Transient Condition ◽  
Type I ◽  
Rt Pcr ◽  
Normal Range ◽  
History Of ◽  
Polymerase Chain ◽  
Time Of Presentation ◽  
Electrocardiogram Ecg

The Brugada-like ECG pattern is a transient condition which may be induced by number of conditions. In this case reported, a 53-year-old Iranian male patient, presented with 7-day history of fever, chills, headache and shortness of breath. At the time of presentation to the Emergency Department (ED), the patient was febrile with tachycardia, tachypnea, and oxygen (O2) saturation 75%. The chest radiograph showed a bilateral infiltration and positive result of Real Time Polymerase Chain Reaction (RT-PCR) confirmed the diagnosis of COVID-19 infection. He received Kaletra, Hydroxychloroquine, Azithromycin, Antibiotics, and Intravenous Immunoglobulin (IVIG). The patients was intubated and underwent mechanical ventilation because of no improvement on the O2 saturation level. Fever subsided on 7th day and Hydroxychloroquine was stopped on 5th day. On the sixth day of hospital stay, Electrocardiogram (ECG) showed sinus tachycardia. Troponin-I- high sensitive was within normal range and Transthoracic Echocardiography (TTE) showed normal Ejection Fraction (EF). On 10th day, type I Brugada ECG pattern presented and regular ECG monitoring was recommended. Finally, after eleven-day hospital stay, the patient died due to bradycardia and asystole despite Cardiopulmonary Resuscitation (CPR). Thus, physicians should consider Brugada ECG pattern in the precordial leads (V1 to V3) of ECG among patients with COVID-19 infection beside regular QT interval monitoring.

scholarly journals The use of chest ultrasonography in suspected cases of COVID-19 in the emergency department

2021 ◽  
Vol 7 (1) ◽  
pp. FSO635
Author(s):  
Enrico Allegorico ◽  
Carlo Buonerba ◽  
Giorgio Bosso ◽  
Antonio Pagano ◽  
Giovanni Porta ◽  
...  
Keyword(s):  
Lung Ultrasound ◽  
Initial Assessment ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Variable Model ◽  
Chest Ultrasonography ◽  
History Of ◽  
Ultrasound Score ◽  
Polymerase Chain ◽  
Or History

Aim: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus-specific reverse transcriptase-polymerase chain reaction (RT-PCR) represents the diagnostic gold standard. We explored the value of chest ultrasonography to predict positivity to SARS-CoV-2 on RT-PCR in suspected COVID-19 cases. Patients & methods: Consecutive patients with suspect COVID-19 were included if they had fever and/or history of cough and/or dyspnea. Lung ultrasound score (LUSS) was computed according to published methods. Results: A total of 76 patients were included. A 3-variable model based on aspartate transaminase (AST) > upper limit of normal, LUSS >12 and body temperature >37.5°C yielded an overall accuracy of 91%. Conclusion: A simple LUSS-based model may represent a powerful tool for initial assessment in suspected cases of COVID-19.

scholarly journals Recurrent deletion in the human antithrombin III gene

Blood ◽  
1991 ◽  
Vol 78 (4) ◽  
pp. 1027-1032
Author(s):  
CB Grundy ◽  
F Thomas ◽  
DS Millar ◽  
M Krawczak ◽  
E Melissari ◽  
...  
Keyword(s):  
Antithrombin Iii ◽  
Direct Sequencing ◽  
Consensus Sequence ◽  
Type I ◽  
Coding Regions ◽  
Human Genes ◽  
History Of ◽  
Polymerase Chain ◽  
Gene Exon ◽  
Recurrent Deletion

Eight unrelated patients with recurrent thromboembolism, a family history of thrombosis, and plasma antithrombin III (ATIII) activity/antigen levels consistent with a diagnosis of heterozygous type I ATIII deficiency were studied by polymerase chain reaction/direct sequencing of ATIII gene exon-coding regions. Frameshift mutations of one base and two bases, respectively, were found to have occurred in two unrelated patients at the same GAG codon (Glu 245) within exon 4 of the ATIII gene. A literature search showed six further hitherto unrecognized deletion “hotspots” in four other human genes. These deletion-prone sites exhibited sufficient sequence homology with each other to derive a consensus sequence (T G A/G A/G G A/C), suggesting that deletion in human genes may not only be non- random but also sequence-directed.

scholarly journals Detection and characterization of seven novel protein S (PROS) gene lesions: evaluation of reverse transcript-polymerase chain reaction as a mutation screening strategy

Blood ◽  
1995 ◽  
Vol 86 (7) ◽  
pp. 2632-2641 ◽  
Author(s):  
CJ Formstone ◽  
AI Wacey ◽  
LP Berg ◽  
S Rahman ◽  
D Bevan ◽  
...  
Keyword(s):  
Splice Site ◽  
Mutation Screening ◽  
Protein S ◽  
Allelic Exclusion ◽  
Type I ◽  
Protein S Deficiency ◽  
Rt Pcr ◽  
S Deficiency ◽  
Polymerase Chain ◽  
Reverse Transcript

Abstract The molecular genetic analysis of protein S deficiency has been hampered by the complexity of the protein S (PROS) gene and by the existence of a homologous pseudogene. In an attempt to overcome these problems, a reverse transcript-polymerase chain reaction (RT-PCR) mutation screening procedure was developed. However, the application of this mRNA-based strategy to the detection of gene lesions causing heterozygous type I protein S deficiency appears limited owing to the high proportion of patients exhibiting absence of mRNA derived from the mutation-bearing allele (“allelic exclusion”). Nevertheless, this strategy remains extremely effective for rapid mutation detection in type II/III protein S deficiency. Using the RT-PCR technique, a G-to-A transition was detected at position +1 of the exon IV donor splice site, which was associated with type I deficiency and resulted in both exon skipping and cryptic splice site utilization. No abnormal protein S was detected in plasma from this patient. A missense mutation (Asn 217 to Ser), which may interfere with calcium binding, was also detected in exon VIII in a patient with type III protein S deficiency. A further three PROS gene lesions were detected in three patients with type I deficiency by direct sequencing of exon-containing genomic PCR fragments: a single base-pair (bp) deletion in exon XIV, a 2-bp deletion in exon VIII, and a G0to-A transition at position-1 of the exon X donor splice site all resulted in the absence of mRNA expressed from the disease allele. Thus, the RT-PCR methodology proved effective for further analysis of the resulting protein S-deficient phenotypes. A missense mutation (Met570 to Thr) in exon XIV of a further type III-deficient proband was subsequently detected in this patient's cDNA. No PROS gene abnormalities were found in the remaining four subjects, three of whom exhibited allelic exclusion. However, the father of one such patient exhibiting allelic exclusion was subsequently shown to carry a nonsense mutation (Gly448 to Term) within exon XII.

scholarly journals Recurrent deletion in the human antithrombin III gene

Blood ◽  
1991 ◽  
Vol 78 (4) ◽  
pp. 1027-1032 ◽  
Author(s):  
CB Grundy ◽  
F Thomas ◽  
DS Millar ◽  
M Krawczak ◽  
E Melissari ◽  
...  
Keyword(s):  
Antithrombin Iii ◽  
Direct Sequencing ◽  
Consensus Sequence ◽  
Type I ◽  
Coding Regions ◽  
Human Genes ◽  
History Of ◽  
Polymerase Chain ◽  
Gene Exon ◽  
Recurrent Deletion

Abstract Eight unrelated patients with recurrent thromboembolism, a family history of thrombosis, and plasma antithrombin III (ATIII) activity/antigen levels consistent with a diagnosis of heterozygous type I ATIII deficiency were studied by polymerase chain reaction/direct sequencing of ATIII gene exon-coding regions. Frameshift mutations of one base and two bases, respectively, were found to have occurred in two unrelated patients at the same GAG codon (Glu 245) within exon 4 of the ATIII gene. A literature search showed six further hitherto unrecognized deletion “hotspots” in four other human genes. These deletion-prone sites exhibited sufficient sequence homology with each other to derive a consensus sequence (T G A/G A/G G A/C), suggesting that deletion in human genes may not only be non- random but also sequence-directed.

The Effects of Different Hydroxyapatite/TiO2 Composite Coatings on Protein Expression of Osteoblast

2009 ◽  
Vol 610-613 ◽  
pp. 1104-1108 ◽  
Author(s):  
Jian Ye Han ◽  
Zhen Tao Yu ◽  
Lian Zhou
Keyword(s):  
Protein Expression ◽  
Type I Collagen ◽  
Composite Coatings ◽  
Type I ◽  
X Ray Diffraction ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Compound Formation ◽  
X Ray ◽  
Polymerase Chain

Hydroxyapatite/TiO2 composite material was coated onto Ti25Nb3Mo2Sn3Zr (TLM) alloy substrate. To study the effects of hydroxyapatite/TiO2 composite coatings on bone-related protein expression, the osteoblast were cultured with composite coatings for different times. The phase transformation and compound formation of the HA/TiO2 coatings were investigated using XRD (X-ray diffraction). The mRNA expression of Type I collagen, alkaline phosphatase (ALP) and osteocalcin were studied by RT-PCR (reverse transcriptional polymerase chain reaction). The titania delayed the crystallization of HA. The mRNA expressions of Type I collagen are decreased as the increasing of TiO2 percentage. The mRNA expressions of osteocalcin are approached. The ALP expression on H4 coating (HA/TiO2 mol ration is 5) after the osteoblast cultured with composite coating for 6 days is the highest. The increasing of TiO2 amount decreases the bioactivity of the composite coatings.

scholarly journals Telomere lengths in women with early reproductive losses

2019 ◽  
Vol 24 ◽  
pp. 202-207
Author(s):  
N. I. Huleyuk ◽  
D. V. Zastavna ◽  
M. Tyrka
Keyword(s):  
Older Women ◽  
Reproductive Function ◽  
Peripheral Blood Lymphocytes ◽  
Control Group ◽  
Healthy Children ◽  
Rt Pcr ◽  
Reproductive Loss ◽  
Reproductive Losses ◽  
History Of ◽  
Polymerase Chain

Aim. Study of the relative telomere lengths in women with a history of early reproductive losses. Methods. Relative Telomere Length (RTL) was studied in the peripheral blood lymphocytes using a real time polymerase chain reaction (RT-PCR). Results. RTL was studied in 281 women, among which 169 - with early reproductive losses (ERL) (surveyed group) and 112 - have healthy children and no reproductive losses in history (control group). For women under the age of 35, the average RTL value is significantly higher than that of older women, P = 0.003597. In women with ERL, RTL is significantly lower than that of women with a preserved reproductive function, P = 0.0000001. The value of RTL is significantly lower in women with ERL under 35 years compared with control, P = 0.0000001, and is similar to value of RTL in women in the control group at the age of 36, P> 0.05. Conclusions. The telomere lengths is significantly lower in both older women and women with ERL. The similarity of RTLvalue in women with ERL up to 35 years of age and in women with a preserved reproductive function at the age of 36 years testifies to the telomeric theory of reproductive aging. Keywords: telomeres, RT-PCR, women, age, early reproductive loss.

scholarly journals Clinicoepidemiological profile and outcome in patients of covid 19 with normal chest x-ray: study of 51 patients

2020 ◽  
Vol 9 (1) ◽  
pp. 181
Author(s):  
Huma Firdaus ◽  
Nafees Ahmad Khan ◽  
Maqsumi Reza ◽  
Mansoor Ahmad Khan ◽  
Gishnu Krishnan ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Age Group ◽  
Rt Pcr ◽  
X Ray ◽  
Good Outcome ◽  
Number Of Patients ◽  
History Of ◽  
Normal Chest ◽  
Chest X Ray ◽  
Time Of Presentation

Background: Covid 19 was declared a pandemic by WHO on 11 March 2020. Patients usually have pneumonia on chest x-ray at time of presentation however many patients also do not develop pneumonia and have normal chest x-ray.Methods: A total of 51 patients above the age of 15 years diagnosed with covid 19 by RT PCR of nasopharngeal/oropharyngeal samples were included in the study. History of symptoms onset was recorded, chest x-ray and haematological investigations were done of all patients.Results: A total of 51 patients >15 years of age were included in the study. 28 were male and 23 were female patients. Maximum number of patients were in age group 15-30 years. Most common presenting complain was fever (49% patients). Most common comorbidity was diabetes mellitus. There was no mortality reported in patients with normal chest x-ray.Conclusions: We conclude from the current study that patients with normal chest x-ray at the time of presentation have a very good outcome.

Serum Cardiac Biomarkers in Asymptomatic Hemodialysis Patients: Role of Soluble Suppression of Tumorigenicity-2

2021 ◽  
pp. 1-8
Author(s):  
Nicoletta Mancianti ◽  
Barbara Maresca ◽  
Marco Palladino ◽  
Gerardo Salerno ◽  
Patrizia Cardelli ◽  
...  
Keyword(s):  
Troponin I ◽  
Predictive Ability ◽  
Small Variation ◽  
Left Ventricular ◽  
Cardiac Biomarkers ◽  
Normal Range ◽  
Coronary Syndrome ◽  
Before And After ◽  
History Of

<b><i>Introduction:</i></b> Cardiovascular events (CVE) remain the leading cause of mortality in hemodialysis (HD) patients. The ability to assess the risk of short-term CVE is of great importance. Soluble suppression of tumorogenicity-2 (sST2) is a novel biomarker that better stratifies risk of CVE than troponins in patients with heart failure. Few studies have investigated the role of sST2 in the HD population. The aim of this single-center study was to assess the predictive ability of sST2 on CVE in comparison to high-sensitive cardiac troponin I (hs-cTnI) and B-type natriuretic peptide (BNP) in HD patients. <b><i>Methods:</i></b> This study used a prospective, observational cohort design. We enrolled 40 chronic HD patients asymptomatic for chest pain and without recent history of acute coronary syndrome. We tested sST2 pre-/post-HD, hs-cTnI, and BNP. Demographic/dialytic/echocardiographic data were evaluated. We recorded the number of CVE for 12 months. The patients were classified into 2 groups: those who developed CVE and those who did not. <b><i>Results:</i></b> Ten of the 40 patients (25%) developed CVE during a 12-month follow-up. Increased sST2 levels (<i>p</i> &#x3c; 0.0001) as well as hs-cTnI and BNP are predictive of CVE. When analyzing biomarkers as binary variables for values above or below the normal range, the correlation remained significant only for sST2 (<i>p</i> = 0.001). A small variation in sST2 levels before and after HD sessions was found (−2.1 ng/mL). sST2 was correlated with left ventricular (LV) echocardiographic data: LV mass index (<i>p</i> = 0.0001), LV ejection fraction (<i>p</i> = 0.01), and diastolic bulging of septum (<i>p</i> = 0.015). BNP and sST2 combination increased the prediction of CVE in a statistical model. <b><i>Conclusion:</i></b> Our study confirms that sST2 is useful for stratifying CV risk in the HD population. sST2 can be evaluated simply as a dichotomous value higher or lower than the normal range, making it easily interpretable. Dialysis and residual diuresis did not affect significantly sST2. A multimarker approach that incorporates sST2 and BNP may improve the prediction of CVE.

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