Potential of Flamboyant Flower (Delonix regia (Boj. ex Hook) Raf) Extract to Maintain Post-Dilution Quality of Aceh Cattle (Bos taurus indicus) Spermatozoa

The objective of this research was to investigate the effect of flamboyant flower extract as a natural antioxidant on sperm quality of Aceh cattle after dilution and equilibration. Fresh semen was collected from 2 healthy bull aged 3.5-4 years old. The research took place in BIB Laboratory. The method used was CRD (Completed Randomized Design), with 6 treatments and 5 replications each The treatments (m/v) were 0%, 0.05%, 0.10%, 0.15% and 0.20% of flamboyant flower extracts. The method used was CRD (Completed Randomized Design), with 6 treatments and 5 replications each. The treatments (m/v) were 0%, 0.05%, 0.10%, 0.15% and 0.20% of flamboyant flower extracts. The data obtained were analyzed by analysis of variance (ANOVA). Parameters evaluated in this study were motility, viability, plasma membrane integrity, and abnormality. Results showed that there was an insignificant effect (p>0.05) of flamboyant flower extract on the quality of Aceh cattle spermatozoa. The best percentage of motility and viability, 80.0% and 88.4%, respectively, were found at P3 (0.15% flamboyant flower extract). In can be concluded from the research that the addition of 0.15% (m/v) D. regia flower extract was able to maintain sperm quality of Aceh cattle to be used in the artificial insemination program.

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Cryopreservation of Aceh Swamp Buffalo (Bubalus bubalis) Semen with Combination of Glycerol and Lactose

Biosaintifika Journal of Biology & Biology Education ◽

10.15294/biosaintifika.v9i3.11426 ◽

2017 ◽

Vol 9 (3) ◽

pp. 409

Author(s):

Kartini Eriani ◽

Nisa Sari ◽

Rosnizar Rosnizar ◽

Dasrul Dasrul ◽

Suhartono Suhartono ◽

...

Keyword(s):

Artificial Insemination ◽

Membrane Integrity ◽

Bubalus Bubalis ◽

Genetic Quality ◽

Plasma Membrane Integrity ◽

Randomized Design ◽

Swamp Buffaloes ◽

Swamp Buffalo ◽

Fresh Semen

Aceh swamp buffaloes (Bubalus bubalis) are decreasing their population and genetic quality. This study was conducted to determine the influence of lactose and glycerol cryoprotectants on spermatozoa of Aceh swamp buffaloes after thawing. This study used completelya factorial randomized design with nine treatments, and five replications. Fresh semen of the Aceh swamp buffalo were diluted by using a combination extender lactose cryoprotectants 0 mM (L0), 60 mM (L60), 120 mM (L120) and glycerol 3% (G3), 5% (G5), 7% (G7) with the equilibration of 4 hours. The results showed that the combination of cryoprotectants L120G7 influenced significantly (P < 0.05) on the quality of spermatozoa of the Aceh swamp buffalo (B. bubalis) after thawing. The percentage of sperm motility L120G7 (42.60 ± 1.14); viability L120G7 (55.00 ± 0.71); acrosome integrity L120G7 (52.00 ± 0.71); and plasma membrane integrity L120G7 (53.20 ± 1.48). The combination of lactose cryoprotectants 120 mM (L120) and glycerol 7% (G7) was the best combination to maintain the quality of spermatozoa of swamp buffalo. This finding could be used to define a policy for the spermatozoa storage of Aceh swamp buffalo to artificial insemination (AI).

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Effect of Incubation Time During Sperm Sexing Process on Sperm Quality of Pasundan Bull

Jurnal Ilmu Ternak dan Veteriner ◽

10.14334/jitv.v25i3.2494 ◽

2020 ◽

Vol 25 (3) ◽

pp. 112

Author(s):

Siti Darodjah Rasad ◽

Nurcholidah Solihati ◽

Kikin Winangun ◽

Annisa Yusrina ◽

Fahmy Avicenna

Keyword(s):

Plasma Membrane ◽

Incubation Time ◽

Lower Layer ◽

Sperm Quality ◽

Membrane Integrity ◽

Dna Integrity ◽

Test Parameter ◽

Plasma Membrane Integrity ◽

Randomized Design

The research was conducted to evaluate the effect of incubation time on viability, plasma membrane integrity, abnormality, and DNA integrity of sexed Pasundan’s bulls sperm. The sperm sexing used 5% and 10% concentrations of Bovine Serum Albumin (BSA). A completely randomized design with three treatments and six replications was used in this study. The data were analyzed using variance analysis followed by Duncan’s multiple distance test. Parameter evaluated were sperm longevity, plasma membrane integrity (PMI), abnormality, and DNA integrity of sexed Pasundan bulls sperm. Results showed that incubation time gave significant effect (P<0.05) on the longevity of sperm, but not on the PMI of Pasundan bulls sexed sperm. The incubation time of 45 minutes gave the highest value of longevity sperm on the upper layer (4.33 days) and the lower layer (4.17 days). Furthermore, the abnormality of sperm X in the upper layer was 4.00%-4.20% and the lower layer was 4.10%- 4.40%. Meanwhile, the DNA integrity of an upper layer was 98.16%-98.66%, and the lower layer was 97.83%-98.58%. It is concluded that 45 minutes of incubation time significantly affected the longevity of sperm, but not plasma membrane integrity, abnormality, and DNA integrity of Pasundan bulls sexed sperm.

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The Effect of Glutathione on The Quality of Aceh Local Catfish (Clarias gariepinus) Spermatozoa After Cryopreservation

Biosaintifika Journal of Biology & Biology Education ◽

10.15294/biosaintifika.v12i1.22353 ◽

2020 ◽

Vol 12 (1) ◽

pp. 125-131

Author(s):

Raudhah Mahfudhah ◽

Kartini Eriani ◽

Zainal Abidin Muchlisin ◽

Cut Ruhul Muthmainnah

Keyword(s):

Plasma Membrane ◽

Clarias Gariepinus ◽

Membrane Integrity ◽

Dna Integrity ◽

Freezing And Thawing ◽

Randomized Design ◽

Fetal Bovine ◽

Fresh Semen ◽

Different Doses

The cryopreservation process might reduce the quality of spermatozoa due to an increase in the production of reactive oxygen species (ROS) compounds during cooling, freezing, and thawing. The quality of spermatozoa can be maintained by adding glutathione as an exogenous antioxidant into cryo-diluent. This study aimed to examine the effect of the addition of different doses of glutathione in cryopreservation of Aceh Local catfish (Clarias gariepinus) spermatozoa after freezing. The method used was a completely randomized design (CRD) with four treatments and four replications. Fresh semen was diluted in Ringer, 15% DMSO, and 20% Fetal Bovine Serum (FBS) and then added with glutathione antioxidants of 0.0 mgL-1, 0.5 mgL-1, 1.0 mgL-1, and 2.0 mgL-1. The parameters observed in this study were motility, integrity of the plasma membrane, fertility, and DNA integrity. The results showed that the concentration of glutathione had no effect on motility, integrity ofthe plasma membrane, or fertility, but had an effect on DNA integrity. The highest percentage of motility and plasma membrane integrity respectively was 40.50% (P3) and 70.87% (P2). Furthermore, the assessment of DNA integrity showed that there was no DNA fragmentation both treatments and fresh spermatozoa. This research is the first study regarding glutathione supplementation in cryo-diluent of Aceh Local catfish spermatozoa. Finally, the results obtained can provide information about the exact concentration of glutathione in the extender on the quality of spermatozoa of Aceh Local catfish (C. gariepinus) after the cryopreservation process. These results can also increase the success of fertility be used by the seed hall unit and the aquaculture industry to increase the productivity and supply high quality seeds.

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17 EFFECT OF LOCAL TREATMENT OF SEMINAL VESICULITIS ON THE QUALITY OF EQUINE FRESH SEMEN

Reproduction Fertility and Development ◽

10.1071/rdv27n1ab17 ◽

2015 ◽

Vol 27 (1) ◽

pp. 101

Keyword(s):

Semen Quality ◽

Local Treatment ◽

Membrane Integrity ◽

Ringer Lactate ◽

Plasma Membrane Integrity ◽

Progressive Motility ◽

Seminal Parameters ◽

Fresh Semen ◽

Lactate Solution

Stallions affected by seminal vesiculitis present history of infertility or subfertility, ejaculatory disturbance, spread of sexually transmitted pathogens, and changes in semen characteristics, leading to reduced semen quality and longevity. The aim of this study was to evaluate the semen quality of stallions with seminal vesiculitis before and after local treatment. Five stallions with a mean age of 12.4 years diagnosed with seminal vesiculitis were used. The identification of the microorganism involved in the pathogenesis of seminal vesiculitis of each animal was performed by bacterial culture of the seminal vesicles flush with Ringer Lactate solution, performed in duplicate at 1-week intervals. After identification of bacteria was performed, there was susceptibility testing to antibiotic (antibiogram) and the appropriate antibiotic was chosen. The local treatment was performed by endoscopy for 10 consecutive days, and this consisted of flushing with Ringer Lactate solution, followed by infusion of the antibiotic selected. The semen analyses were performed before starting the local treatment for seminal vesiculitis (M0), after a week (M1), and after a month (M2) of therapy. Sperm kinetics were performed by computerized method – CASA for the following parameters: percentage of sperm with total motility, progressive motility, and rapid sperm. Analysis of plasma membrane integrity was performed by epi-fluorescence microscopy, using the combination of fluorescent probes carboxyfluorescein diacetate and propidium iodide. Percentage of leukocytes was assessed through evaluation in light optical microscopy of semen smears stained with DiffQuick. The content of nitric oxide (NO) was determined by colourimetric Griess reaction by a spectrophotometer through the concentrations of nitrate (NO3–) and nitrite (NO2–). To perform the count of colony forming units per millilitre (CFU mL–1), an aliquot of 0.1 mL of semen was diluted in 9.9 mL of saline. A 0.1-mL aliquot of this sample was plated on Mueller-Hinton agar. The seeded plates were incubated, and the bacterial colonies were counted after 24 h. According to the performed dilution, total colonies identified corresponds to ×10 000 CFU mL–1. The data were analysed by two-way ANOVA followed by Tukey's test (P < 0.05). The values (mean ± standard error) of seminal parameters on M0, M1, and M2 were the following, respectively: sperm kinetics (total motility: 46.5 ± 5.13a; 75.1 ± 3.42b; 42.8 ± 5.28a; progressive motility: 19.3 ± 3.86a; 33.4 ± 2.39b; 16.5 ± 2.40a; rapid sperm: 22.2 ± 1.82a; 52.2 ± 5.65b; 22.1 ± 2.62a); plasma membrane integrity (47.5 ± 4.65a; 62.9 ± 5.41b; 39.1 ± 4.32a); percentage of leukocytes (35.2 ± 2.36a; 15.1 ± 2.55b; 36.1 ± 4.04a); CFU (119 980 × 103 ± 19 528.0 × 103a; 5375 × 103 ± 2453.7 × 103b; 65 850 × 103 ± 19 701.0 × 103ab) on fresh semen; and NO content (0.645 ± 0.172a, 0.117 ± 0.023b, 0.364 ± 0.110ab) on seminal plasma. The results demonstrate that local treatment after a week leads to an improvement in sperm quality; however, this was not maintained after 1 month of therapy, since the seminal parameters at this time are similar to pretreatment, which can be justified by recurrent disease.

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O papel do dimetilsulfóxido na criopreservação de sêmen de Curimba (Prochilodus lineatus)

Semina Ciências Agrárias ◽

10.5433/1679-0359.2015v36n5p3471 ◽

2015 ◽

Vol 36 (5) ◽

pp. 3471

Author(s):

Antonio Sergio Varela Junior ◽

Estela Fernandes Silva ◽

Tainã Figueiredo Cardoso ◽

Érica Yokoyama Namba ◽

Rodrigo Desessards Jardim ◽

...

Keyword(s):

Sperm Quality ◽

Membrane Integrity ◽

Fertilization Rate ◽

Dna Integrity ◽

Hatching Rate ◽

Sperm Viability ◽

Prochilodus Lineatus ◽

Dmso Concentration ◽

Fresh Semen

Cryopreservation of Curimba semen (Prochilodus lineatus) is ecological and commercial importance. The objective of this study was to evaluate the effect of different concentrations (2, 5, 8 and 11%) of dimethyl sulfoxide (DMSO) diluted in Betsville Thawing Solution (BTS) on the quality of post-thaw semen Curimba. We analyzed the rate and period motility, sperm viability, membrane integrity and DNA, mitochondrial functionality, and fertilization and hatching rate. The plasma membrane and DNA integrity of a DMSO concentration of 11% obtained better results than the concentration of 5% (p <0.05). However, treatment of 5% DMSO resulted in a longer latency and a higher fertilization rate and hatching, in other sperm quality equal to that of fresh semen. The results of this study indicate that 5% DMSO is ideal for cryopreservation of semen Curimba.

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The Effect of Different Concentrations of Caffeine, Pentoxifylline and 2’-Deoxyadenosine on the Biological Properties of Frozen-Thawed Canine Semen

Annals of Animal Science ◽

10.2478/aoas-2019-0025 ◽

2019 ◽

Vol 19 (3) ◽

pp. 733-746

Author(s):

Marek Lecewicz ◽

Rafał Strzeżek ◽

Anna M. Majewska ◽

Piotr S. Purpurowicz ◽

Władysław Kordan

Keyword(s):

Plasma Membrane ◽

Sperm Motility ◽

Artificial Insemination ◽

Membrane Integrity ◽

Phosphodiesterase Inhibitors ◽

Biological Properties ◽

Kinematic Parameters ◽

Plasma Membrane Integrity ◽

Beneficial Effects

AbstractArtificial insemination (AI) and semen cryopreservation are the most accessible and commonly used techniques for breeding domestic animals. Among many parameters, such as plasma membrane integrity and acrosome structure, one of the key factors that determine the quality of frozen-thawed samples for artificial insemination is sperm motility. Sperm motility is one of the key parameters that determine the quality of frozen-thawed samples for AI. The total number of progressively motile spermatozoa in thawed canine semen is correlated with fertility. A variety of substances were used to compare sperm motility with the control. The aim of this study was to determine the effect of semen extender supplementation with motility stimulants, pentoxifylline (PTX), caffeine (CAF) and 2’-deoxyadenosine (DX), after different post-thaw incubation times (30, 60, 120 min) on the motility, selected kinematic parameters, plasma membrane integrity and mitochondrial membrane potential of cryopreserved canine spermatozoa. During attempts to improve the quality of cryopreserved semen, the applied substances exerted beneficial effects at a concentration of 10 mM. We demonstrated that both phosphodiesterase inhibitors, caffeine and pentoxifylline, as well as 2’-deoxyadenosine increased the motility and selected kinematic parameters of thawed canine spermatozoa.

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The effects of Trolox on the quality of sperm from captive squirrel monkey during liquefaction in the extender ACP-118™

Zygote ◽

10.1017/s096719941800028x ◽

2018 ◽

Vol 26 (4) ◽

pp. 333-335 ◽

Cited By ~ 2

Author(s):

D. V. C. Almeida ◽

J. S. Lima ◽

D. L. Leão ◽

K. G. Oliveira ◽

R. R. Santos ◽

...

Keyword(s):

Plasma Membrane ◽

Squirrel Monkey ◽

Sperm Motility ◽

Membrane Integrity ◽

Plasma Membrane Integrity ◽

Time Periods ◽

Semen Extender ◽

Fresh Semen

SummaryThe aim of this study was to evaluate the effect of incubating semen for different periods (90, 270 or 450 min) with or without Trolox® (100 or 150 µM) on the quality of sperm from Saimiri collinsi. Sperm motility, vigour, and plasma membrane integrity (PMI) were evaluated in both fresh semen and semen incubated for different time periods, i.e. 90, 270 or 450 min of incubation. Supplementation of semen extender with Trolox® 100 µM improved sperm motility, vigour and PMI for up to 270 min of incubation.

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Membrane Integrity Test for Spermatozoa in Simmental Bull Liquid Semen Using the Addition of Gentamycin and Sweet Orange Essential Oil in the Extender

Asian Journal of Advanced Research and Reports ◽

10.9734/ajarr/2020/v8i430206 ◽

2020 ◽

pp. 42-46

Author(s):

Sukma Aditya Sitepu ◽

Julia Marisa

Keyword(s):

Essential Oil ◽

Sweet Orange ◽

Membrane Integrity ◽

Egg Yolk ◽

Integrity Test ◽

Randomized Design ◽

Simmental Cattle ◽

Fresh Semen ◽

Orange Essential Oil

Aims: This study aimed to determine the quality of Simmental Bull liquid semen through laboratory tests, including the percentage value of membrane integrity Spermatozoa with the addition of gentamycin and sweet orange essential oil to tris yolk extender. Methodology: The material used in this research is Simmental Cattle bull fresh semen, tris yolk extender, gentamicin, and sweet orange essential oil. Tris yolk extender was prepared with Tris (hydroxymethyl aminomethane) (3.32 g), citric acid (1.86 g), fructose (1.37 g), glycerol (6 ml), egg yolk (20 ml), aquades (100 ml). The experimental design used in the study was a nonfactorial Complete Randomized Design with five treatments and five replicates. The treatment given is the addition of sweet orange essential oil 0%, 0.25%, 0.5%, 0.75% and 1%. The parameter observed was the evaluation of membrane integrity before equilibration and after equilibration. Results: The results showed that the percentage of membrane integrity in Simmental bull liquid semen continues to increase with the addition of sweet orange essential oils. The results showed that the addition of sweet orange essential oil as much as 0% produced a membrane integrity percentage after equilibration of 63%, adding 0.25% resulted in 68%, adding 0.5% yielded 70%, adding 0.75% yielded 75% and adding 1% resulted in 79%. Conclusion: The best percentage of membrane integrity in Simmental bull liquid semen was the addition of gentamicin and sweet orange essential oil in the tris yolk extender at the level of 1%.

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Quality of Sexed Sperm of Bali Bull in Regional Artificial Insemination Center of Riau Province

Buletin Peternakan ◽

10.21059/buletinpeternak.v43i2.34357 ◽

2019 ◽

Vol 43 (2) ◽

Author(s):

Yendraliza Yendraliza ◽

Anwar Efendi Harap ◽

July Handoko ◽

Muhammad Rodiallah

Keyword(s):

Plasma Membrane ◽

Pregnancy Rate ◽

Artificial Insemination ◽

Membrane Integrity ◽

Egg Yolk ◽

Female Offspring ◽

Plasma Membrane Integrity ◽

Frozen Semen ◽

Calving Rate

This study aimed to evaluate the quality of frozen semen of Bali bull resulted from sexing procedure on calf or offspring production with desired sex. The tested sperm of Bali bull were collected from Bali bull raised at Regional Artificial Insemination Center of Riau Province (BIBD Riau). The study was carried out in 2 stages. The first stage was X and Y chromosome separation by albumin method. The extender used in the sexing procedure is trice citrate fructose and egg yolk. The second stage was mainly testing the sexed sperm collected in 60 Bali cow in Langkat Village, Bengkalis Regency. To determine the quality of post thawing frozen semen collected from the sexing procedure, the study evaluated motility, viability, mortality, abnormality and plasma membrane integrity of the spermatozoa. The pregnancy rate, calving rate, and birth accuracy of inseminated sexed sperm to offspring’ sex were also evaluated. The evaluation resulted in motility (66.3-75.3%), viability (70-78.5%), plasma membrane integrity (60-65.8%), abnormality (6.05-8.05%), mortality (20.05-30.05%), and pregnancy rate (83.33-90%). The calving rate on this study was 100% with the birth accuracy of 81.8% for male offspring and 40% for female offspring. As conclusion, the sexed sperm evaluated on this study have fairly good fertility.

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A Comparison of Freezing Methods and Diluents Types on Post-Thaw Sperm Quality of Ram Sperm

Research in Agriculture Livestock and Fisheries ◽

10.3329/ralf.v7i2.48863 ◽

2020 ◽

Vol 7 (2) ◽

pp. 235-241

Author(s):

Pankaj Kumar Jha ◽

M Golam Shahi Alam ◽

Farida Yeasmin Bari

Keyword(s):

Plasma Membrane ◽

Sperm Quality ◽

Membrane Integrity ◽

Egg Yolk ◽

Sperm Parameters ◽

Plasma Membrane Integrity ◽

One Step ◽

Acrosome Integrity ◽

Ram Sperm

The effect of freezing methods and diluents types on post-thaw sperm quality of Bangladeshi ram semen was studied. Two freezing methods and three diluents was tested as pooling effects (freezing methods or diluents) on post-thaw sperm parameters; sperm motility (SM), viability (SV), plasma membrane integrity (SPMI) and acrosome integrity (SAI), respectively. From selected ten rams, eight ejaculates were used for each freezing group (freezing methods × diluents). Semen samples were diluted by using two-steps for hand-made tris-based diluents (20% egg yolk): D1 (7% glycerol) and D2 (5% glycerol), and one-step dilution for commercial diluents: D3 (Triladyl®) at 35°C. After 4h of equilibration of temperature at 5°C, diluted semen samples was aspirated into 0.25 mL straws, and sealed. Straws were frozen in liquid nitrogen (LN2) vapour using two methods: F1 (manually in Styrofoam box, using three-steps method; +5°C to -80°C at -11.33°C/min, -80°C to -120°C at -26.66°C/min, and -120°C to -140°C at - 13.33°C/min) and F2 (programmable bio-freezer, using two-steps method; +5°C to -100°C at - 20°C/min and -100°C to -140°C at -10°C/min). Two semen straws from each batch were evaluated (37°C for 20 sec) for sperm parameters. In pool effects between freezing methods; SAI differed significantly (P < 0.001). The SM (56%) and SV (72%) were observed competitive. However, SPMI (67.58 ± 2.02%) and SAI (76.13 ± 1.42%) were higher in F1. Among diluents, SM (P < 0.006), SV (P < 0.008), SPMI (P < 0.012) and SAI (P < 0.019) differed significantly. The SM (61.25 ± 1.80%), SV (77.13 ± 1.47%), SPMI (68.31 ± 1.91%) and SAI (74.75 ± 1.64%) were highest in D3. In conclusion, the combination of manual freezing (three-steps) and handmade tris-based diluents (20% egg yolk, 5% glycerol) is suitable and sustainable method for cryopreservation of ram semen. Res. Agric., Livest. Fish.7(2): 235-241, August 2020

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