Effects of Cnidoscolus aconitifolius on Some Hematological Parameters of Male Wistar Rats

Introduction: Medicinal plants have become increasingly useful as a form of alternative therapy. Cnidoscolus aconitifolius is a medicinal plant applied in folklore remedies in the treatment and prevention of various diseases. Aim: The aim of this study is to assess the effects of Cnidoscolus aconitifolius on some hematological parameters of male Wistar rats. Methodology: A total of 15 male Wistar rats weighing between 100-250 g were randomly placed in groups. Group 1 served as control and received distilled water only; group 2 received 200 mg/kg and group 3 received 400 mg/kg of the hydromethanolic leaf extract of Cnidoscolus aconitifolius. Extract was administered once daily using oro-gastric cannula for 30 days. Blood samples were collected by direct cardiac puncture into appropriate sample tubes for estimation of hematological parameters including red blood cell count (RBC), packed cell volume (PCV), haemoglobin (Hb) concentration, white blood cell count (WBC) and platelet count. Results: Results showed a significant (P<0.05) increase in RBC, PCV, Hb and platelet count with the higher dose of 400 mg/kg body weight of the extract compared to control group. However, the WBC count was not significantly (P>0.05) altered. Conclusion: Oral administration of the leaf extract of Cnidoscolus aconitifolius increases red blood cell count, packed cell volume, hemoglobin level and platelet count at 400 mg/kg body weight.

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Biochemical and hematological parameters in chronic kidney disease

Journal of Manmohan Memorial Institute of Health Sciences ◽

10.3126/jmmihs.v4i1.21132 ◽

2018 ◽

Vol 4 (1) ◽

pp. 4-11

Author(s):

S. Singh ◽

S. Bhatta

Keyword(s):

Chronic Kidney Disease ◽

Platelet Count ◽

Kidney Disease ◽

Blood Cell ◽

Biochemical Parameters ◽

Leukocyte Count ◽

Hematological Parameters ◽

Total Leukocyte Count ◽

Blood Cell Count ◽

Red Blood Cell Count

Background: Chronic kidney disease is a worldwide public health problem. It is associated with various biochemical and hematological abnormalities that leads to morbidity and mortality. The aim of this study was to evaluate the biochemical and hematological parameters in chronic kidney disease patients.Methods: This is a prospective cross sectional study conducted over a period of six months on 52 chronic kidney disease patients at Kist Medical College Teaching Hospital, Lalitpur, Nepal. Biochemical parameters such as urea, creatinine, calcium, phosphorous, sodium, potassium and hematological parameters like hemoglobin, hematocrit, red blood cell count, total leukocyte count, platelet count were measured using standard techniques in chronic kidney disease cases and the findings were compared with age and sex matched controls. Results were analyzed using SPSS 21 for Windows.Results: Hemoglobin, hematocrit, red blood cell count, total leukocyte count and platelet count were reduced and statistically significant in chronic kidney disease patients compared to controls(p <0.05).In biochemical parameters, serum urea, creatinine, phosphorous were increased and calcium was reduced which was found statistically significant as compared to controls (p <0.05). The combination of diabetes and hypertension was the lead cause of chronic kidney disease found in 38.46% followed by hypertension alone in 30.76%Conclusion: Biochemical and hematological parameters are deranged in patients with chronic kidney disease. Routine evaluation of these parameters is useful in the management of these patients.

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Association between body size and selected hematological parameters in men and women aged 45 and above from a hospitalized population of older adults: an insight from the Polish Longitudinal Study of Aging (1960–2000)

Anthropological Review ◽

10.1515/anre-2017-0012 ◽

2017 ◽

Vol 80 (2) ◽

pp. 171-190 ◽

Cited By ~ 2

Author(s):

Piotr Chmielewski ◽

Bartłomiej Strzelec ◽

Jolanta Chmielowiec ◽

Krzysztof Chmielowiec ◽

Krzysztof Borysławski

Keyword(s):

Older Adults ◽

Body Size ◽

Blood Cell ◽

Cell Count ◽

Red Blood Cell ◽

Hematological Parameters ◽

Body Height ◽

Blood Cell Count ◽

Duodenal Ulcers ◽

Red Blood Cell Count

AbstractIn elderly people, anemia occurs with increasing frequency with each advancing decade and can be a harbinger of very serious health conditions, including gastrointestinal bleeding, gastric and duodenal ulcers, and cancer. Therefore, age-dependant changes in hematological parameters deserve special attention. Nonetheless, very few longitudinal studies of aging have focused on possible associations between basic anthropometric characteristics and hematological parameters in older people. Here, we present some evidence that body size can be associated with red blood cell count as well as some other selected hematological parameters in adults aged 45 to 70 years. Longitudinal data on anthropometric and hematological parameters have been obtained from physically healthy residents at the Regional Psychiatric Hospital for People with Mental Disorders in Cibórz, Lubuskie Province, Poland (142 individuals, including 68 men and 74 women). The residents who took psychoactive drugs were excluded from the study. To evaluate the studied relationships, three anthropometric traits were used and three dichotomous divisions of the study sample were made. The medians of body height, body weight, and body mass index at the age of 45 years were used to divide the sample into: shorter and taller, lighter and heavier, and slimmer and stouter individuals, respectively. Student’s t-test, Pearson’s correlation, and regression analysis were employed. The results of the present study suggest that the relationship between body size and red blood cell count is slightly more pronounced in men and its strength depends on age. However, the correlations between body size and red blood cell count proved to be weak in both sexes. With aging, the strength of the relation decreased gradually, which might have been caused by the aging-associated changes in the hematopoietic system, anemia, or was an artifact. Further studies are needed to elucidate the unclear association between body size and hematological parameters in older adults.

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Fresh Human Blood as the Source of a Regional Quality Survey Programme in Haematology

Annals of Clinical Biochemistry International Journal of Laboratory Medicine ◽

10.1177/000456328201900610 ◽

1982 ◽

Vol 19 (6) ◽

pp. 438-441 ◽

Cited By ~ 1

Author(s):

J M W A Van Gend

Keyword(s):

Platelet Count ◽

Blood Cell ◽

Cell Count ◽

Human Blood ◽

Red Blood Cell ◽

Packed Cell Volume ◽

Blood Cell Count ◽

Blood Samples ◽

Red Blood Cell Count ◽

Edta Anticoagulated Blood

In a regional quality survey programme in haematology with a limited number of participants (15 in the region described), it appeared possible to use unpreserved, fresh, EDTA-anticoagulated blood samples. With such samples only the results from hospital laboratories with different instruments and methods could adequately be compared. From the results of single analyses obtained in ten different samples, the systematic differences as well as the precision of the analyses were calculated for each hospital laboratory with respect to haemoglobin, packed cell volume, red blood cell count, white blood cell count, and platelet count.

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Enoxaparin-induced reactive thrombocytosis: a case report

Thrombosis Journal ◽

10.1186/s12959-021-00290-x ◽

2021 ◽

Vol 19 (1) ◽

Author(s):

Tao Xiang ◽

Ming Cheng

Keyword(s):

Molecular Weight ◽

Platelet Count ◽

Blood Cell ◽

Cell Count ◽

White Blood Cell Count ◽

White Blood Cell ◽

Hip Replacement ◽

Low Molecular Weight ◽

Blood Cell Count ◽

Reactive Thrombocytosis

Abstract Background Enoxaparin is an anticoagulant that falls in the class of medications called low molecular weight heparins (LMWHs), and is used to prevent or treat patients with deep vein thrombosis (DVT) and pulmonary embolism. Enoxaparin is the most widely used LMWH for DVT prophylaxis following knee or hip replacement surgery. Common side effects of enoxaparin include bleeding, petechiae at the injection site, and thrombocytopenia. However, reactive thrombocytosis is a rarely reported adverse reaction. We managed a patient who developed enoxaparin-associated thrombocytosis, which was completely resolved after treatment cessation. Case presentation A 78-year-old female was hospitalized for post-hip replacement rehabilitation. Low molecular weight heparin 40 mg/day was administered subcutaneously to prevent deep venous thrombosis (DVT). At admission, her platelet count was normal (228 × 109/L) and her white blood cell count was slightly elevated (12.91 × 109/L). Seven days after admission, the patient developed thrombocytosis, which peaked on the 14th day (836 × 109/L), while her white blood cell count had returned to normal (8.86 × 109/L). Her therapeutic regimen was reviewed, and enoxaparin was identified as a potentially reversible cause of reactive thrombocytosis. Switching from enoxaparin to rivaroxaban lead to a gradual decrease in the patient’s platelet count, which eventually returned to normal levels 16 days after enoxaparin was discontinued. No complications secondary to thrombocytosis was observed, and no conclusion was reached on the use of small doses of aspirin for antithrombotic therapy under these circumstances. Conclusion Enoxaparin-induced reactive thrombocytosis should be suspected in patients with thrombocytosis following enoxaparin administration as an anticoagulant to prevent certain complications.

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Diagnostic value of post-operative platelet-to-white blood cell ratio after splenectomy in patients with advanced ovarian cancer

International Journal of Gynecological Cancer ◽

10.1136/ijgc-2019-000712 ◽

2019 ◽

Vol 29 (8) ◽

pp. 1292-1297 ◽

Cited By ~ 4

Author(s):

Konstantinos Lathouras ◽

Georgios Panagakis ◽

Sarah Joanne Bowden ◽

Konstantinos Saliaris ◽

Srdjan Saso ◽

...

Keyword(s):

Ovarian Cancer ◽

Platelet Count ◽

Blood Cell ◽

Cell Count ◽

White Blood Cell Count ◽

White Blood Cell ◽

Predictive Value ◽

Blood Cell Count ◽

Cell Ratio ◽

Grade Iii

IntroductionSplenectomy-induced thrombocytosis and leukocytosis may obscure the early diagnosis of post-operative infection or sepsis. In trauma patients after splenectomy, a platelet-to-white blood cell ratio of <20 has been shown to reliably differentiate post-operative sepsis from transient physiological responses.ObjectiveTo determine whether the platelet-to-white blood cell ratio can be applied to differentiate between reactive post-operative changes and latent infection.MethodsAll consecutive patients with ovarian cancer who underwent splenectomy between January 2013 and October 2018 in two large European gynecological cancer centers were retrospectively evaluated. Main outcome measures were white blood cell count, platelet count, and platelet-to-white blood cell ratio on post-operative days 1, 5, and 7. These were correlated with surgical outcome and morbidity according to the Clavien-Dindo classification. A binomial logistic regression was applied to assess the predictive value of day 5 platelet-to-white blood cell ratio, white blood cell count, and platelet count for predicting grade III post-operative sepsis.ResultsNinety-five patients with ovarian cancer (mean age 54 years, range 18–75) were identified. Seventeen patients (17.9%) developed a grade III post-operative sepsis. In all post-operative patients, mean white blood cell count on day 5 decreased (from 15.4×103/μL to 11.4×103/μL), while the mean platelet count rose (from 260.7×103/μL to 385.3×103/μL). A high platelet count (>313×103/μL) failed to show any predictive value (OR=0.94; 95% CI 0.30 to 3.0; p=0.921). A low platelet-to-white blood cell ratio (<26) (OR=3.49; 95% CI 1.18 to 10.32; p=0.0241) and high white blood cell count (>14.5×103/μL) on day 5 (OR=11.0; 95% CI 3.3 to 36.2; p<0.001) were significant for predicting sepsis. Despite a significant OR, the sensitivity and specificity were low; day 5 platelet-to-white blood cell ratio at a cut-off point of 26 achieved a sensitivity of 72% and specificity of 53% (area under the curve 0.637, 95% CI 0.480 to 0.796) in predicting grade III post-operative sepsis.ConclusionsPlatelet-to-white blood cell ratio after cytoreductive surgery for ovarian cancer with splenectomy does not appear to have a strong predictive value in differentiating between sepsis and reactive splenectomy-induced changes. Leukocytosis, in combination with clinical assessment, may remain the most useful tool for prediction of sepsis after cytoreductive surgery with splenectomy.

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Effect of plateletpheresis on hematocrit, hemoglobin and erythrocyte count: Meta-analysis 1980–2018

Scientific Reports ◽

10.1038/s41598-019-56175-7 ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 2

Author(s):

Alejandro Gil-Betacur ◽

Carmen Yulieth Mantilla-Gutiérrez ◽

Jaiberth Antonio Cardona-Arias

Keyword(s):

Blood Cell ◽

Cell Count ◽

Red Blood Cell ◽

Meta Analysis ◽

Erythrocyte Count ◽

Blood Cell Count ◽

Selection Processes ◽

Negative Effect ◽

Red Blood Cell Count ◽

The Mean

AbstractThe effects of platelet donation by apheresis on different parameters of the erythrogram are still unclear. The objective was to meta-analyze the effect of plateletpheresis on hematocrit, hemoglobin, and erythrocyte count, with a systematic review with random effects meta-analysis of the mean difference. The PRISMA guidelines were considered, as well as 133 search strategies on four different databases. Reproducibility was guaranteed and methodological quality was evaluated. Heterogeneity was evaluated with Galbraith and DerSimonian-Laird’s, publication bias with a funnel plot and a Begg’s test, sensitivity analysis and a cumulative meta-analysis were also conducted. Eighteen (18) articles were included, 17 evaluated the effects on hematocrit in 2,564 donors; 13 on hemoglobin in 1,640 donors; and 4 on red blood cell count in 243 donors. A decrease of 2.26% (CI95% = 2.11–2.41) was observed in hematocrit, of 0.80 g/dL (CI95% = 0.75–0.86) in hemoglobin and −0.21 × 1012/L (CI95% = −0.13; −0.29) in red blood cell count. Plateletpheresis has a negative effect on the erythrogram parameters, explained by blood loss in the kits used for the procedure and cell lysis. Such evidence is relevant to secure the efficiency and safety of the procedure, improve selection processes or determine the number of donations that can be performed without affecting donors’ health.

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