scholarly journals Clinical Background and Detection of Chlamydia trachomatis Antigen among Infertile Women Attending University of Maiduguri Teaching Hospital, Nigeria

2020 ◽  
pp. 20-26
Author(s):  
A. S. Kumurya ◽  
M. Usman ◽  
S. J. Sheriff ◽  
H. Ngabra ◽  
M. A. Umar ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
Teaching Hospital ◽  
Transmitted Disease ◽  
Rapid Test ◽  
Nucleic Acid Amplification ◽  
Full Time ◽  
Chlamydia Trachomatis Infection ◽  
Sexually Transmitted ◽  
Infertile Women ◽  
Clinical Background

Background: Chlamydia trachomatis infection is an established cause of pelvic inflammatory disease (PID), ectopic pregnancy, chronic pelvic pain and infertility among women. It is the most common bacterial sexually transmitted disease in the world. The infection is largely asymptomatic. Aim: The study was carried out to determine the Clinical background as well as detect the Chlamydia trachomatis antigen among infertile women attending University of Maiduguri Teaching Hospital (UMTH). Methods: A survey on the Clinical background and antigens to Chlamydia trachomatis was carried out among infertile and post natal women attending fertility Clinic UMTH. A total of 65 endocervical swab samples were collected from the aforementioned group of subjects within the age of 18-47 years, out of which 45 were collected from infertile women while the remaining 20 were obtained from post-natal women to serve as control subjects. The samples were analysed by using Chlamydia Rapid Test device swab/urine (Abon Biopharm(Hangzhou), Co., Ltd) that work on the principle of immunochromatographic technique. Results: Highest number of infertility was observed on women within the age of 30-35 years (33.3%). The result shows that those that attained tertiary educational level have the highest number of infertility (60%). The study demonstrated highest number of infertility among female civil servants (53.3%) followed by full-time house wife (33.3%). More infertility was observed among women who are not engaged in the use of contraceptive measures (95.6%). The overall result from this study shows no prevalence (0.0%) of C. trachomatis genital infection among infertile women in UMTH. Conclusion: Further research using more sensitive and specific procedures for the detection of C. trachomatis from clinical specimens such as nucleic acid amplification tests and cell culture are recommended among infertile women in Borno State, Nigeria.

scholarly journals 183. chlamydia Trachomatis Seroprevalence with a pgp3 Serologic Assay and Association with Pelvic Inflammatory Disease Among Women, United States, 2013–2016

2020 ◽  
Vol 7 (Supplement_1) ◽  
pp. S220-S220
Author(s):  
Gloria E Anyalechi ◽  
Damien Danavall ◽  
Brian H Raphael ◽  
Katherine E Bowden ◽  
Jaeyoung Hong ◽  
...  
Keyword(s):  
Black Women ◽  
Chlamydia Trachomatis ◽  
Pelvic Inflammatory Disease ◽  
Inflammatory Disease ◽  
White Women ◽  
Transmitted Disease ◽  
Population Level ◽  
Reproductive Age ◽  
Nucleic Acid Amplification ◽  
Sexually Transmitted

Abstract Background Chlamydia trachomatis (CT) causes pelvic inflammatory disease (PID) and other sequelae; however, these associations are not fully characterized. CT serologic assays including Pgp3 ELISA may detect prior CT infection and may better elucidate these associations. We used a serologic Pgp3 multiplex bead array assay (Pgp3MBA) to measure CT seroprevalence in reproductive-age US women and assess the association with PID. Methods We performed CT Pgp3MBA on sera collected from women 18–39 years old during the 2013–2016 cycles of the National Health and Nutrition Examination Survey (NHANES) who had available urine CT nucleic acid amplification test results. Weighted Pgp3MBA CT seroprevalence and 95% confidence intervals (95% CI) were calculated. We also determined weighted prevalence ratios (PRs) and 95% CIs of self-reported lifetime PID among women with and without detectable Pgp3MBA and other characteristics to estimate these US national statistics. Results Among 2,339 women, 1,725 (73.7%) had available sera. Of these women, 1,425 (or 93.4% of those with data) were sexually experienced and had a CT seroprevalence of 35.9% (95% CI 33.4–38.4). When weighted for US women, CT seroprevalence was 30.5% (95% CI 26.6–34.4%), ranging from 16.9% (95% CI 11.0–22.8%) among non-Hispanic Asian women to 70.2% (95% CI 62.4–78.0%) among non-Hispanic black women. PID was reported by 4.2% (95% CI 3.1–5.2) of 1,413 sexually-experienced women with PID data or an estimated 3.8% (95% CI 2.6–5.0) of US women. Among US women, estimated PID varied by Pgp3MBA status; 7.3% (95% CI 4.3–10.2) of Pgp3MBA-positive women were estimated to report PID versus 2.3% (95% CI 1.3–3.4) of Pgp3MBA-negative women (PR 3.1; 95% CI 1.7–5.9). PID prevalence did not vary by age, nor self-reported recent sexually transmitted disease among US women, but was higher among non-Hispanic black women compared to non-Hispanic white women (PR 2.2; 95% CI 1.4–3.5). Conclusion Nearly one-third of US women have had CT by Pgp3MBA, with differences by race/ethnicity. Women with prior CT had three times the reported PID prevalence of women without CT. Further serologic research may refine the population-level impact of CT prevention activities, such as recommended annual CT screening, on PID incidence, particularly among non-Hispanic black women. Disclosures All Authors: No reported disclosures

scholarly journals Comparison of Performances of Two Commercially Available Tests, a PCR Assay and a Ligase Chain Reaction Test, in Detection of Urogenital Chlamydia trachomatis Infection

1998 ◽  
Vol 36 (6) ◽  
pp. 1489-1493 ◽  
Author(s):  
Mirja Puolakkainen ◽  
Eija Hiltunen-Back ◽  
Timo Reunala ◽  
Satu Suhonen ◽  
Pekka Lähteenmäki ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
Clinical Laboratory ◽  
Transmitted Disease ◽  
Chain Reaction ◽  
Ligase Chain Reaction ◽  
Chlamydia Trachomatis Infection ◽  
Sexually Transmitted ◽  
Transport Medium ◽  
Sexually Transmitted Disease Clinic ◽  
Chain Reaction Test

The diagnostic performance of a PCR test (Roche Cobas Amplicor CT/NG Test) and that of a ligase chain reaction (LCR) test (Abbott LCxChlamydia trachomatis assay) were compared by using endocervical and urethral swab specimen culture as a reference test. First-void urine (FVU) and endocervical and urethral swab specimens were collected from 1,015 unselected patients attending a sexually transmitted disease clinic and a clinic for adolescents in Helsinki, Finland. Chlamydia trachomatis was cultured from samples from the endocervix or urethra. PCR was performed with fresh and frozen urine and the culture transport medium. LCR was performed with fresh and frozen urine and LCx swab transport medium. Diagnostic consistency and diagnostic accuracy were statistically tested. The test results were identical for 984 patients (97%). Discrepant results were observed for 31 patients. Overall, LCR and PCR showed excellent kappa coefficients of consistency for both swab and FVU specimens (0.93 and 0.95, respectively). Sixty-one patients (6%) were culture positive. Testing of FVU by LCR or PCR increased the overall positivity rates to 7.0 and 7.7%, respectively. While PCR of FVU detected the greatest number of C. trachomatis infections (sensitivity, 96.1%), for some PCR-positive FVU specimens the results could not be confirmed (specificity, 99.6%). PCR and LCR were more sensitive than culture (sensitivities, 92 and 93% versus 79% for culture) in the diagnosis of genital C. trachomatis infection. In conclusion, both tests can be recommended for use in the clinical laboratory and for the screening of asymptomatic C. trachomatis infections.

scholarly journals Genital chlamydia trachomatis infection: an underestimated sexually transmitted disease in China

2020 ◽  
Author(s):  
Xinlong Chen ◽  
Yanling Gan ◽  
Qiu Chen ◽  
Zhuo Ren ◽  
Furong Li ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
Sexually Transmitted Disease ◽  
Laboratory Testing ◽  
Laboratory Tests ◽  
Transmitted Disease ◽  
Shandong Province ◽  
Chlamydia Trachomatis Infection ◽  
Sexually Transmitted ◽  
Reported Data ◽  
Antigen Testing

Abstract Background Genital chlamydia trachomatis infection is one of the most prevalent sexually transmitted disease globally. The diagnosis and reporting of genital chlamydia trachomatis infection mainly rely on effective laboratory testing available. The prevalence of genital chlamydia trachomatis infection in China may be largely underestimated, based on the reported data. Methods A survey was conducted among hospitals provided clinical services for STD in Shandong Province in 2018. Basic information on the hospitals, type of laboratory tests provided and cases reported of GCTI were collected and analysed in SPSS 22. Results Among 456 hospitals surveyed, antigen testing, PCR testing, and either antigen or PCR testing were available among 200 (43.9%), 88 (19.3%) and 268 (58.8%) hospitals, respectively. PCR testing was more available among tertiary and secondary hospitals than primary hospitals (X 2 =28.560, P=0.000). Significant differences were observed in the availability of antigen testing, PCR testing, and either antigen or PCR testing among different type of hospitals (X 2 =15.708, P=0.003; X 2 =22.494, P=0.000; X 2 =21.729, P=0.000). In 2018, a total of 1532 cases of GCTI were reported in 99 hospitals. More cases were reported by tertiary and secondary hospitals than primary hospitals (X 2 =24.082, P=0.000). The proportion of different kind of hospitals that reported case of GCTI cases was consistent with that of hospitals provided laboratory testing for GCTI. Conclusion The availability of laboratory testing for GCTI in Shandong province was poor, which may lead to underestimation of prevalence of GCTI. More effort needs to improve the availability of GCTI laboratory testing in order to effectively control of GCTI in Shandong province.

scholarly journals Prevalence of Genital Chlamydia Trachomatis among Pregnant Women in a Northwestern Teaching Hospital, Nigeria

2020 ◽  
Author(s):  
Rabi'at Aliyu Muhammad ◽  
Adebiyi Gbadebo Adesiyun ◽  
Shafaatu Ismail Sada ◽  
Shamsudin Aliyu ◽  
Abdulhakeem Abayomi Olorukooba
Keyword(s):  
Chlamydia Trachomatis ◽  
Pregnant Women ◽  
Teaching Hospital ◽  
Sexually Transmitted Infection ◽  
Tertiary Education ◽  
Nucleic Acid Amplification ◽  
World Health ◽  
Categorical Variables ◽  
Transmitted Infection ◽  
Sexually Transmitted

Abstract Background: Chlamydia trachomatis (Ct) is now being considered as an “obstetric pathogen” due to its potential in causing adverse pregnancy and perinatal outcomes. Consequent to being the commonest bacterial sexually transmitted infection, screening is recommended at the initial visit of antenatal care, but few countries have routine pregnancy screening and treatment programs. Prevalence assessment is a core component of the World Health Organisation sexually transmitted infection -surveillance programming which enables a country to monitor trends of this infection, for appropriate implementation of preventive measures. Aim: To determine the prevalence of Ct infection among pregnant women, and to describe sociodemographic characteristics and reproductive profile of seropositive pregnant women attending the antenatal clinic of Ahmadu Bello University Teaching Hospital, Zaria. Methods: Socio-demographic characteristics and risk factors were sought from 90 pregnant women using a proforma. Their sera were assayed for the presence of Ct immunoglobulin G (IgG) using ELISA. Data were analysed using SPSS version 21. Categorical variables were represented using frequency and percentages while associations between categorical variables were determined using Fisher’s exact test with p < 0.05 considered significant. Results: Prevalence of Ct infection was found to be 3.3% (3/90). The mean age of infected women was 34.3 ± 4.6 years. All seropositive women were gainfully employed and had tertiary education. Mean parity was 3, the majority (2/3,66.7%) had no previous miscarriage and none had a history of prior stillbirth.Conclusion: The prevalence of Ct in this study is low compared to the pooled prevalence for the West African sub-region. A broader community-based study using a nucleic acid amplification technique is suggested.

Evaluation of an Enzyme Immunoassay for Detection of Chlamydia Trachomatis in Urogenital Specimens

1990 ◽  
Vol 1 (1) ◽  
pp. 49-52 ◽  
Author(s):  
J A J W Kluytmans ◽  
A H van der Willigen ◽  
B Y M van Heyst ◽  
W I van der Meyden ◽  
E Stolz ◽  
...  
Keyword(s):  
Cell Culture ◽  
Chlamydia Trachomatis ◽  
Transmitted Disease ◽  
Rapid Test ◽  
University Hospital ◽  
Male Population ◽  
Sexually Transmitted ◽  
High Risk Populations ◽  
Male Patients ◽  
The University

Chlamydiazyme® (Abbott), an enzyme-linked immunoassay (EIA), was evaluated using cell culture on Hela 229 cells as the method of reference. Samples were acquired from 611 female and 280 male patients attending the outpatient clinic for sexually transmitted disease at the University Hospital in Rotterdam, The Netherlands. The prevalences of chlamydia culture-positive female and male patients were 7.8% and 14.4% respectively. The overall sensitivity and specificity values of the EIA were respectively 68.1% and 95.8% in the female and 92.1% and 92.0% in the male population. Samples which were culture-negative but EIA-positive were re-examined by a second direct test (IDEA; Boots Celltech). If the samples from 12 females and 11 males which were negative on culture but positive with both direct tests are considered as failures of cell culture, the sensitivity of the EIA in females almost equalled cell culture (74.6% versus 79.9%) and in males was even higher (93.9% versus 77.6%). Serotyping of the cultured strains revealed that all serovars of Chlamydia trachomatis occurring in this study could be detected by the EIA. The EIA offers a relatively simple and rapid test for diagnosis of C. trachomatis infections in high-risk populations.

scholarly journals How Many Patients Who Present to North Central Bronx Hospital with a Sexually Transmitted Disease (Chlamydia, Gonorrhea) Are Tested for HIV?

2017 ◽  
Vol 4 (suppl_1) ◽  
pp. S106-S106
Author(s):  
Lok Yung
Keyword(s):  
Chlamydia Trachomatis ◽  
Hiv Testing ◽  
Health Care Providers ◽  
Transmitted Disease ◽  
Rapid Test ◽  
Care Providers ◽  
Data Synthesis ◽  
North Central ◽  
Sexually Transmitted ◽  
Hiv Test

Abstract Background To determine whether an individual got infected with either Chlamydia trachomatis, N gonorrhea, or both infected, the HIV test was ordered or not. Methods Systematic review and data synthesis of STD and HIV testing results through Quardamed within the 1 month period of November, 2015. Percentage of patients with and without a STD (either Chlamydia trachomatis, N gonorrhea, or both infected) who had HIV test done. Results 804 patients were tested for gonorrhea or chlamydia by GC probe during November 2015. Thirty-three of 804 (4.1%) individuals were infected with Chlamydia trachomatis. Of those 20 were tested for HIV by rapid oral test. Six of 804 (0.7%) participants who were infected with N gonorrhea had five HIV rapid test done. Two of 804 (0.3%) participants were infected with both diseases and one patient was tested for HIV. Most patients with a confirmed STD were diagnosed in ED (43.4%) followed by OB/GYN (37.3%). Seventy-two percent of patients who were either infected with chlamydia, gonorrhea or both were tested for HIV. In the ED, 48% had an HIV test vs. 80% in OB/GYN (including family planning). Conclusion The majority of patients were tested for HIV. More patients were tested in OB/GYN compared with the ED likely because an HIV counselor is readily available during clinic hours. Increase in education and training of health care providers and nurses to become more comfortable in testing patients for HIV may increase the number HIV testing when patients present with STDs. Disclosures All authors: No reported disclosures.

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