scholarly journals Diagnosis of Hordeum vulgare Genomic Profile: Review

2021 ◽  
pp. 32-42
Author(s):  
Zeina S. M. Al-Hadeithi ◽  
Saade Abdalkareem Jasim
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Hordeum Vulgare ◽  
Plant Genetic Resources ◽  
Genetic Material ◽  
Hordeum Spontaneum ◽  
Genetic Fingerprinting ◽  
Breeding Programs ◽  
Dna Library ◽  
Long Time

This review represent plants genetic diversity (PDG) generally in crop plant and especially in Barley  (Hordeum vulgare), can be studied (PDG) and stored as a (PGR) plant genetic resources as gene bank , DNA library for saved genetic material at long time and crops improvement can be utilized in breeding programs strategies in future. In this  study observed the significance of plant genetic diversity (PGD) and (PGR) especially on agriculturally important crops , analysis of plant genomic using molecular markers. Barley is a well important studies crops using as a model for study genetic plant, cultivated barley Hordeum vulgare easily hybridization by genetic fingerprinting with wiled barley Hordeum spontaneum. The molecular markers showed their relation with locus of geographic factors and imposed stresses. Here, discussed barley genomic through relationship between genotype and phenotype traits using molecular markers useful for genetic physiological maps construction.

scholarly journals Genome Size: A Novel Predictor of Nut Weight and Nut Size of Walnut Trees

HortScience ◽  
2018 ◽  
Vol 53 (3) ◽  
pp. 275-282 ◽  
Author(s):  
Saadat Sarikhani Khorami ◽  
Kazem Arzani ◽  
Ghasem Karimzadeh ◽  
Abdolali Shojaeiyan ◽  
Wilco Ligterink
Keyword(s):  
Genetic Diversity ◽  
Genome Size ◽  
Plant Genetic Resources ◽  
Kernel Weight ◽  
High Yield ◽  
Phenotypic Analysis ◽  
High Genetic Diversity ◽  
Breeding Programs ◽  
Superior Genotypes ◽  
Southwest Of Iran

Plant genetic diversity is the fundamental of plant-breeding programs to improve desirable characteristics. Hence, evaluation of genetic diversity is the first step in fruit-breeding programs. Accordingly, the current study was carried out to evaluate 25 superior walnut genotypes in respect of phenotypic and cytological characteristics. For this purpose, 560 walnut genotypes in southwest of Iran were evaluated based on UPOV and International Plant Genetic Resources Institute (IPGRI) descriptor. After a 2-year primary evaluation, 25 superior genotypes were selected for future phenotypic and genome size assessment. Flow cytometry was used to estimate genome size of the selected superior genotypes. A high genetic diversity was found in walnut population collected from the southwest of Iran. The selected superior genotypes had high yield, lateral bearing, thin-shell thickness (0.90–1.64 mm), high nut (12.54–19.80 g) and kernel (7.02–9.91 g) weight with light (L) to extra light (EL) kernel color which easily can be removed from the shell. Also, FaBaCh2 genotype turned out to be protogynous being important as a pollinizer cultivar. In addition to extensive phenotypic analysis, genome size was determined. The studied genotypes were diploid (2n = 2x = 32) and varied in genome size from 1.29 (FaBaAv2) to 1.40 pg (FaBaNs12). Correlation analysis showed that lateral bearing, budbreak date, nut size, and weight were the main variables contributing to walnut production. A linear relationship was found between genome size and nut weight (r = 0.527**), kernel weight (r = 0.551**), and nut size index (NSI) (r = 0.487**). Therefore, genome size can be considered as a strong and valuable tool to predict nut and kernel weight and nut size.

Genetic diversity and population structure of Algerian chickpea (Cicer arietinum) genotypes: use of agro-morphological traits and molecular markers linked or not linked to the gene or QTL of interest

2020 ◽  
Vol 71 (2) ◽  
pp. 155
Author(s):  
Djihad Bellemou ◽  
Teresa Millàn ◽  
Juan Gil ◽  
Aissa Abdelguerfi ◽  
Meriem Laouar
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Cicer Arietinum ◽  
Morphological Traits ◽  
Ascochyta Blight ◽  
Principal Component ◽  
Breeding Programs ◽  
Average Value ◽  
Conservation Of Genetic Resources ◽  
Resistant Genotypes

Assessment of genetic diversity among chickpea (Cicer arietinum L.) germplasm at the morphological and molecular levels is fundamental for chickpea breeding and conservation of genetic resources. Genetic variability of 46 chickpea genotypes including 42 Algerian genotypes and four control varieties was evaluated by using 15 agro-morphological traits. Eleven molecular markers including nine simple sequence repeats, one sequence characterised amplified region (SCY17) and one gene-specific (CaETR4) were used to characterise the 46 genotypes and eight references varieties added for disease resistance or susceptibility. Genotypes resistant to ascochyta blight were identified by the markers SCY17 and CaETR4 present together. High diversity was observed for all measured morphological traits between genotypes. Yield components, plant height, phenological traits and growth habit were the traits most involved in variation among genotypes and were partitioned into four groups by using principal component analysis. All molecular markers were polymorphic. In total, 91 alleles were obtained ranging from 2 to 21 per locus with average of 8.27 alleles per marker. Polymorphism information content ranged from 0.58 to 0.99 with an average value of 0.87. UPGMA clustering and Bayesian-based model structure analysis grouped genotypes into two clusters, but the distribution of the genotypes by cluster was not the same for the two analyses. According to the presence of markers indicating resistance to ascochyta blight (SCY17 and CaETR4), three resistant genotypes (FLIP 82-C92, ILC 6909, ILC 7241) were selected and should be tested in controlled conditions for confirmation. Considering the narrow diversity of cultivated chickpea, the Algerian genotypes can be considered as interesting for future breeding programs.

scholarly journals Importance of Genetic Diversity Assessment in Crop Plants and Its Recent Advances: An Overview of Its Analytical Perspectives

2015 ◽  
Vol 2015 ◽  
pp. 1-14 ◽  
Author(s):  
M. Govindaraj ◽  
M. Vetriventhan ◽  
M. Srinivasan
Keyword(s):  
Genetic Diversity ◽  
Food Production ◽  
Genetic Manipulation ◽  
Plant Genetic Resources ◽  
Crop Improvement ◽  
Genetic Material ◽  
Easy Access ◽  
Production Network ◽  
Gene Banks ◽  
Diversity Assessment

The importance of plant genetic diversity (PGD) is now being recognized as a specific area since exploding population with urbanization and decreasing cultivable lands are the critical factors contributing to food insecurity in developing world. Agricultural scientists realized that PGD can be captured and stored in the form of plant genetic resources (PGR) such as gene bank, DNA library, and so forth, in the biorepository which preserve genetic material for long period. However, conserved PGR must be utilized for crop improvement in order to meet future global challenges in relation to food and nutritional security. This paper comprehensively reviews four important areas; (i) the significance of plant genetic diversity (PGD) and PGR especially on agriculturally important crops (mostly field crops); (ii) risk associated with narrowing the genetic base of current commercial cultivars and climate change; (iii) analysis of existing PGD analytical methods in pregenomic and genomic era; and (iv) modern tools available for PGD analysis in postgenomic era. This discussion benefits the plant scientist community in order to use the new methods and technology for better and rapid assessment, for utilization of germplasm from gene banks to their applied breeding programs. With the advent of new biotechnological techniques, this process of genetic manipulation is now being accelerated and carried out with more precision (neglecting environmental effects) and fast-track manner than the classical breeding techniques. It is also to note that gene banks look into several issues in order to improve levels of germplasm distribution and its utilization, duplication of plant identity, and access to database, for prebreeding activities. Since plant breeding research and cultivar development are integral components of improving food production, therefore, availability of and access to diverse genetic sources will ensure that the global food production network becomes more sustainable. The pros and cons of the basic and advanced statistical tools available for measuring genetic diversity are briefly discussed and their source links (mostly) were provided to get easy access; thus, it improves the understanding of tools and its practical applicability to the researchers.

Genetic Diversity Assessment and its Importance on Crop improvement in Ethiopia: Potentials and challenges

2017 ◽  
Vol 23 (1) ◽  
Author(s):  
Obssi Dessalegn Hora ◽  
Abebaw Misganaw Amebaw
Keyword(s):  
Genetic Diversity ◽  
Food Production ◽  
Crop Production ◽  
Plant Genetic Resources ◽  
Crop Improvement ◽  
Genetic Material ◽  
Germplasm Conservation ◽  
Global Perspective ◽  
Easy Access ◽  
Diversity Assessment

Genetic diversity assessments of plant play a great role in a predictable area to improve agricultural production and productivity, to solve food uncertainty in developing world.  Many breeders has tried to  realized that crop with diverge genetic diversity  can be assessed , evaluated ,captured and stored in the form of superior  plant  genetic resources  such as gene bank, DNA library to  preserve genetic material for long period. However, the conserved genetically diversified plant must be utilized to improve crop production in order to solve future food and nutritional challenges. This paper reviews eight important areas; (i) Gaps in Developing Taxonomy of Ethiopian crops (ii) Monitoring diversity for crop improvement, (iii) Alterations in landscape features, (iv) Significance of Germplasm Conservation of crops, (v) Gap in morphological characterization, (vi) Global perspective of agro biodiversity and molecular evolution, (vii) Emergence of tissue culture technology in Ethiopia (viii) Germplasm improvement. It provides basic enlightenment for plant breeders for better understanding and rapid diversity assessment of crop, for better understanding and utilization of germplasm from gene banks to their applied breeding programs. With the advent of new biotechnological techniques, this process of conventional breeding is now being accelerated and carried out with more precision and speedy manner than the classical breeding techniques by using molecular markers to avoid taxonomic confusion. For sustainable food production, conventional plant breeding research should have integration with molecular marker assisted evaluation of crops genetic diversity and/or cultivar improvement will be achieved. As a result, availability and access to diverse genetic sources will ensure that the global food production network becomes more sustainable. The merit and demerit of the basic morphological characterizations are briefly discussed and their source links were provided to get easy access; thus, it improves the understanding of modern molecular tools and its practical applicability to the breeders. 

scholarly journals Breeding potential and genetic diversity of "Híbrido do Timor" coffee evaluated by molecular markers

2010 ◽  
Vol 10 (4) ◽  
pp. 298-304 ◽  
Author(s):  
Tesfahun Alemu Setotaw ◽  
Eveline Teixeira Caixeta ◽  
Guilherme Ferreira Pena ◽  
Eunize Maciel Zambolim ◽  
Antonio Alves Pereira ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Cluster Analysis ◽  
Molecular Markers ◽  
Genetic Structure ◽  
Gene Diversity ◽  
High Genetic Diversity ◽  
Breeding Programs ◽  
Information Index ◽  
Upgma Cluster Analysis ◽  
Potential Use

AFLP, RAPD and SSR molecular markers were used to study the genetic diversity and genetic structure of the Híbrido de Timor germplasm. The principal coordinate analysis, UPGMA cluster analysis based on genetic dissimilarity of Jaccard, Bayesian model-based cluster analysis, percentage of polymorphic loci, Shannon's information index and Nei gene diversity were employed to assess the genetic diversity. The analyses demonstrated a high genetic diversity among Híbrido de Timor accessions. UPGMA and Bayesian cluster analyses grouped the accessions into three clusters. The genetic structure of Híbrido de Timor is reported. The management of Híbrido de Timor germplasm variability and its potential use in breeding programs are discussed.

scholarly journals Maximizing genetic representation in seed collections from populations of self and cross-pollinated banana wild relatives

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Simon Kallow ◽  
Bart Panis ◽  
Dang Toan Vu ◽  
Tuong Dang Vu ◽  
Janet Paofa ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Papua New Guinea ◽  
Mating Systems ◽  
Plant Genetic Resources ◽  
Genetic Material ◽  
New Guinea ◽  
Wild Relatives ◽  
Ex Situ ◽  
Conservation Targets ◽  
Seed Collections

Abstract Background Conservation of plant genetic resources, including the wild relatives of crops, plays an important and well recognised role in addressing some of the key challenges faced by humanity and the planet including ending hunger and biodiversity loss. However, the genetic diversity and representativeness of ex situ collections, especially that contained in seed collections, is often unknown. This limits meaningful assessments against conservation targets, impairs targeting of future collecting and limits their use. We assessed genetic representation of seed collections compared to source populations for three wild relatives of bananas and plantains. Focal species and sampling regions were M. acuminata subsp. banksii (Papua New Guinea), M. balbisiana (Viet Nam) and M. maclayi s.l. (Bougainville, Papua New Guinea). We sequenced 445 samples using suites of 16–20 existing and newly developed taxon-specific polymorphic microsatellite markers. Samples of each species were from five populations in a region; 15 leaf samples from different individuals and 16 seed samples from one infructescence (‘bunch’) were analysed for each population. Results Allelic richness of seeds compared to populations was 51, 81 and 93% (M. acuminata, M. balbisiana and M. maclayi respectively). Seed samples represented all common alleles in populations but omitted some rarer alleles. The number of collections required to achieve the 70% target of the Global Strategy for Plant Conservation was species dependent, relating to mating systems. Musa acuminata populations had low heterozygosity and diversity, indicating self-fertilization; many bunches were needed (> 15) to represent regional alleles to 70%; over 90% of the alleles from a bunch are included in only two seeds. Musa maclayi was characteristically cross-fertilizing; only three bunches were needed to represent regional alleles; within a bunch, 16 seeds represent alleles. Musa balbisiana, considered cross-fertilized, had low genetic diversity; seeds of four bunches are needed to represent regional alleles; only two seeds represent alleles in a bunch. Conclusions We demonstrate empirical measurement of representation of genetic material in seeds collections in ex situ conservation towards conservation targets. Species mating systems profoundly affected genetic representation in seed collections and therefore should be a primary consideration to maximize genetic representation. Results are applicable to sampling strategies for other wild species.

scholarly journals Genetic diversity and population structure of Eurycoma apiculata in Eastern Sumatra, Indonesia

2021 ◽  
Vol 22 (10) ◽  
Author(s):  
Zulfahmi Zulfahmi ◽  
Parjanto Parjanto ◽  
Edi Purwanto ◽  
Ahmad Yunus
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Genetic Variation ◽  
Genetic Differentiation ◽  
Gene Diversity ◽  
In Situ Conservation ◽  
Genetic Material ◽  
Ex Situ ◽  
Breeding Programs ◽  
The Mean

Abstract. Zulfahmi, Parjanto, Purwanto E, Yunus A. 2021. Genetic diversity and population structure of Eurycoma apiculata in Eastern Sumatra, Indonesia. Biodiversitas 22: 4431-4439. Information on genetic variation within and among populations of Eurycoma apiculata plants is important to develop strategies for their conservation, sustainable use, and genetic improvement. To date, no information on genetic variation within and among populations of the E. apiculata has been reported. This study aims to assess genetic diversity within and among populations of E. apiculata based on RAPD markers, and to determine populations to collect E. apiculata genetic material for conservation and breeding programs. Young leaves of E. apiculata were collected from six natural populations. Fifteen RAPD primers were used to assess the genetic diversity of each population. The data obtained were analyzed with POPGEN and Arlequin software. The amplification results of 15 selected primers produced 3-16 loci with all primers 100% polymorphic. At the species level, the mean allele per locus (Na), number of effective alleles (Ne), percentage of polymorphic loci (PPL), Nei’s gene diversity index (He) and Shannon information index (I) were 2.000, 1.244, 100%, 0.167, and 0.286, respectively. At the population level, the mean values for Na, Ne, PPL, He and I were 1.393, 1.312, 39.27%, 0.119, and 0.186, respectively. The highest value of gene diversity within population (He) was found in the Lingga-1 population and the lowest value was found in the Rumbio population. The value of genetic differentiation among populations (GST) of E. apiculata is 0.284, consistent with the results of the AMOVA analysis which found that genetic variation among populations was 23.14%, indicates that the genetic variation of E. apiculata was more stored within populations than among populations. The gene flow (Nm) value of E. apiculata was 1.259 migrants per generation among populations. The Nm value of this species was high category, and could inhibit genetic differentiation among populations. The clustering of E. apiculata population based on the UPGMA dendrogram and PCA was inconsistent with its geographic distribution, reflecting the possibility that genes migration occurred between islands in the past. The main finding of this study was the genetic variation of the E. apiculata mostly stored within the population. Therefore, the population with the highest genetic diversity is a priority for in-situ conservation, and collection of E. apiculata genetic material for ex-situ conservation and breeding programs should be carried out minimum from Lingga-1 and Pokomo populations.

scholarly journals Introducing Beneficial Alleles from Plant Genetic Resources into the Wheat Germplasm

Biology ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 982
Author(s):  
Shivali Sharma ◽  
Albert Schulthess ◽  
Filippo Bassi ◽  
Ekaterina Badaeva ◽  
Kerstin Neumann ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Resources ◽  
Plant Genetic Resources ◽  
Wheat Breeding ◽  
Breeding Programs ◽  
Selection Processes ◽  
Starting Point ◽  
The Status ◽  
Modern Wheat ◽  
Elite Germplasm

Wheat (Triticum sp.) is one of the world’s most important crops, and constantly increasing its productivity is crucial to the livelihoods of millions of people. However, more than a century of intensive breeding and selection processes have eroded genetic diversity in the elite genepool, making new genetic gains difficult. Therefore, the need to introduce novel genetic diversity into modern wheat has become increasingly important. This review provides an overview of the plant genetic resources (PGR) available for wheat. We describe the most important taxonomic and phylogenetic relationships of these PGR to guide their use in wheat breeding. In addition, we present the status of the use of some of these resources in wheat breeding programs. We propose several introgression schemes that allow the transfer of qualitative and quantitative alleles from PGR into elite germplasm. With this in mind, we propose the use of a stage-gate approach to align the pre-breeding with main breeding programs to meet the needs of breeders, farmers, and end-users. Overall, this review provides a clear starting point to guide the introgression of useful alleles over the next decade.

Identification of research to improve the efficiency of breeding strategies for white clover in Australia - a review

2002 ◽  
Vol 53 (3) ◽  
pp. 239 ◽  
Author(s):  
M. Z. Z. Jahufer ◽  
M. Cooper ◽  
J. F. Ayres ◽  
R. A. Bray
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
White Clover ◽  
Molecular Breeding ◽  
Computer Modelling ◽  
Future Research ◽  
Specific Reference ◽  
Breeding Strategies ◽  
Breeding Programs ◽  
Conventional Breeding

A major challenge faced by today’s white clover breeder is how to manage resources within a breeding program. It is essential to utilise these resources with sufficient flexibility to build on past progress from conventional breeding strategies, but also take advantage of emerging opportunities from molecular breeding tools such as molecular markers and transformation. It is timely to review white clover breeding strategies. This background can then be used as a foundation for considering how to continue conventional plant improvement activities and complement them with molecular breeding opportunities. In this review, conventional white clover breeding strategies relevant to the Australian dryland target population environments are considered. Attention is given to: (i) availability of genetic variation, (ii) characterisation of germplasm collections, (iii) quantitative models for estimation of heritability, (iv) the role of multi-environment trials to accommodate genotype-by-environment interactions, (v) interdisciplinary research to understand adaptation to dryland environments, (vi) breeding and selection strategies, and (vii) cultivar structure. Current achievements in biotechnology with specific reference to white clover breeding in Australia are considered, and computer modelling of breeding programs is discussed as a useful integrative tool for the joint evaluation of conventional and molecular breeding strategies and optimisation of resource use in breeding programs. Four areas are identified as future research priorities: (i) capturing the potential genetic diversity among introduced accessions and ecotypes that are adapted to key constraints such as summer moisture stress and the use of molecular markers to assess the genetic diversity, (ii) understanding the underlying physiological/morphological root and shoot mechanisms involved in water use efficiency of white clover, with the objective of identifying appropriate selection criteria, (iii) estimation of quantitative genetic parameters of important morphological/physiological attributes to enable prediction of response to selection in target environments, and (iv) modelling white clover breeding strategies to evaluate the opportunities for integration of molecular breeding strategies with conventional breeding programs.

Sign in / Sign up

Export Citation Format

Share Document