Genetic Characterization of Domestic Apple Varieties from Tuzla Canton, BiH

Aims: The basic precondition for apple breeding is the genetic diversity of varieties, which implies a large number of different, positive genes that enable adaptation to different weather conditions, resistance to new diseases and pests. One of the reliable sources of genetic diversity are indigenous varieties of Bosnia and Herzegovina. Their genetic identification is the first step in a process that has as its ultimate goal the collection of genetic material. The main goal of this research is to analyze the genetic variability of five indigenous varieties of apple in Tuzla Canton, Bosnia and Herzegovina in terms of contributing to the management of conservation and expansion of existing genetic resources, spreading this material through registered nurseries. Study Design: The research included fiveautochthonous apple varieties: "Ovčji nos“ “Dobrić“, "Šarenika“, "Rančica“ and "Petrovača“. Place and Duration of Study: Samples of young leaves were collected at the site of Donji Moranjci, City of Srebrenik,Tuzla Canton, Bosnia and Herzegovina in the spring of 2019. Methodology: In order to determine the genetic diversity of five indigenous apple varieties were genotyped ten SSR(Simple Sequence Repeats) markers. Results: No cases of synonyms or homonyms were found within the analyzed set.The results of the study indicate a pronounced differentiation, ie all five examined varieties represent unique genotypes. Conclusion: The examined sets of genotypes possess significant genetic variability, which is important especially when we consider that a relatively small number of samples have been analyzed.

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Genetic Variability of Autochthonous Pear Varieties from Tuzla Canton, Bosnia and Herzegovina

European Journal of Biology and Biotechnology ◽

10.24018/ejbio.2021.2.5.235 ◽

2021 ◽

Vol 2 (5) ◽

pp. 87-91

Author(s):

Besim Salkić ◽

Ensar Salkić ◽

Amela Hercegovac ◽

Aldijana Avdić ◽

Azra Dorić ◽

...

Keyword(s):

Genetic Variability ◽

Bosnia And Herzegovina ◽

Genetic Resource ◽

Agronomic Traits ◽

Quality Characteristic ◽

Fruit Species ◽

Young Leaves ◽

The Town ◽

Unique Genotype ◽

Simple Sequence

Pear as a fruit species has a special place because of its quality characteristic. The fact that about 20,000 pear seedlings are considered to be sold annually in Bosnia and Herzegovina, which are autochthonous or spontaneously expanded varieties, also speaks in favor. The main goal of this research is to analyze the genetic variability of nine autochthonous pear varieties in Tuzla Canton, Bosnia and Herzegovina to enable the conservation and expansion of existing genetic resources. The study included nine autochthonous pear varieties. Samples of young leaves were collected on the following localities: the town of Srebrenik, the town of Gradačac, the municipality of Čelić-Tuzla Canton, Bosnia and Herzegovina, in the spring 2019. To determine genetic diversity, 12 SSR (Simple Sequence Repeats) markers were genotyped. There were no cases of synonyms or homonyms in the analyzed set. Each of the nine tested varieties represents a unique genotype. Autochthonous pear cultivars analyzed in this paper represent an interesting genetic resource, with useful agronomic traits that can be used in future cultivation.

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ISSR Marker Based Genetic Diversity in Morinda Spp. For Its Enhanced Collection, Conservation and Utilization

10.21203/rs.3.rs-666059/v1 ◽

2021 ◽

Author(s):

Lalit Arya ◽

Ramya Kossery Narayanan ◽

Anjali Kak ◽

Chitra Devi Pandey ◽

Manjusha Verma ◽

...

Keyword(s):

Genetic Diversity ◽

Gene Diversity ◽

Inter Simple Sequence Repeat ◽

Issr Markers ◽

Morinda Citrifolia ◽

Species Differentiation ◽

Information Index ◽

Upgma Cluster Analysis ◽

Simple Sequence

Abstract Morinda (Rubiaceae) is considerably recognized for its multiple uses viz. food, medicine, dyes, firewood, tools, oil, bio-sorbent etc. The molecular characterization of such an important plant would be very useful for its multifarious enhanced utilization. In the present study, 31 Morinda genotypes belonging to two different species Morinda citrifolia and Morinda tomentosa collected from different regions of India were investigated using Inter Simple Sequence Repeat (ISSR) markers. Fifteen ISSR primers generated 176 bands with an average of 11.7 bands per primer, of which (90.34%) were polymorphic. The percentage of polymorphic bands, mean Nei’s gene diversity, mean Shannon’s information index in Morinda tomentosa and Morinda citrifolia was [(69.89%, 30.68%); (0.21 ± 0.19, 0.12 ± 0.20); (0.32 ± 0.27 0.17 ± 0.28)] respectively, revealing higher polymorphism and genetic diversity in Morinda tomentosa compared to Morinda citrifolia. Structure, and UPGMA cluster analysis placed the genotypes into well-defined separate clusters belonging to two species Morinda tomentosa and Morinda citrifolia revealing the utility of ISSR markers in species differentiation. Distinct ecotypes within a particular species could also be inferred emphasizing the collection and conservation of Morinda genotypes from different regions, in order to capture the overall diversity of respective species. Further higher diversity of M. tomentosa must be advanced for its utilization in nutraceutical, nutritional and other nonfood purposes.

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Characterization of the Genetic Diversity of Acid Lime (Citrus aurantifolia (Christm.) Swingle) Cultivars of Eastern Nepal Using Inter-Simple Sequence Repeat Markers

Plants ◽

10.3390/plants7020046 ◽

2018 ◽

Vol 7 (2) ◽

pp. 46

Author(s):

Nabin Munankarmi ◽

Neesha Rana ◽

Tribikram Bhattarai ◽

Ram Shrestha ◽

Bal Joshi ◽

...

Keyword(s):

Genetic Diversity ◽

Simple Sequence Repeat ◽

Inter Simple Sequence Repeat ◽

Sequence Repeat ◽

Simple Sequence Repeat Markers ◽

Citrus Aurantifolia ◽

Acid Lime ◽

Simple Sequence

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Genetic Diversity and Characterization of a Core Collection of Malus Germplasm Using Simple Sequence Repeats (SSRs)

Plant Molecular Biology Reporter ◽

10.1007/s11105-011-0399-x ◽

2011 ◽

Vol 30 (4) ◽

pp. 827-837 ◽

Cited By ~ 35

Author(s):

Sarah M. Potts ◽

Yuepeng Han ◽

M. Awais Khan ◽

Mosbah M. Kushad ◽

A. Lane Rayburn ◽

...

Keyword(s):

Genetic Diversity ◽

Simple Sequence Repeats ◽

Core Collection ◽

Simple Sequence

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Polymorphic microsatellites of analysis in cultivars of taro

Horticultura Brasileira ◽

10.1590/s0102-05362012000100018 ◽

2012 ◽

Vol 30 (1) ◽

pp. 106-111 ◽

Cited By ~ 1

Author(s):

Raquel SC Nunes ◽

Fernanda R Pinhati ◽

Luciana P Golinelli ◽

Tiyoko Nair H Rebouças ◽

Vânia Margaret F Paschoalin ◽

...

Keyword(s):

Genetic Diversity ◽

Local Varieties ◽

Mato Grosso ◽

Espírito Santo ◽

Polymorphic Microsatellites ◽

Espirito Santo ◽

Microsatellite Alleles ◽

Mato Grosso Do Sul ◽

Simple Sequence

Taro (Colocasia esculenta) is a tuberous plant belonging to the Araceae family whose tuber is the 14th most consumed food crop in the world. Characterized as an unconventional vegetable, taro is grown in Brazil as a subsistence crop, but in recent years began to gain commercial importance, especially in the states of Espirito Santo, Minas Gerais and Rio de Janeiro. To avoid loss of genetic diversity of the local varieties traditionally grown in Brazil a core collection for taro germplasm has been developed by the Instituto Capixaba de Pesquisa, Assistência Técnica e Extensão Rural do estado do Espirito Santo (Incaper). The aim of this study was to perform a molecular characterization of the seven regional core collections. Genetic diversity of the cultivars was investigated by using SSR (Simple Sequence Repeats) polymorphisms, in seven loci (Xuqtem55, Xuqtem73, Xuqtem84, Xuqtem88, Xuqtem91, Xuqtem97 and Xuqtem110). Genetic diversity of the cultivars, based on the seven microsatellite alleles, was evaluated by using the software GelCompar II, showed that the loci Xuqtem73, Xuqtem88 and Xuqtem110 were the most informative, featuring 7, 10 and 8 alleles, respectively, a percentage of cultivars with polymorphic alleles of 85, 57 and 100% and identical PIC of 0.91. Based on Xuqtem110 locus analysis, the seven cultivars were grouped in two clusters. Chinês Regional Incaper cultivar was originated from Chinês cultivar which originated the São Bento cultivar, corroborating previous results. Macaquinho and Chinês cultivars were shown to be the primitive ones originating the allelic collections found in the states of Mato Grosso do Sul and Espirito Santo.

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Assessment of Genetic Diversity of Rheum species (Endangered Medicinal Herb of Indian Himalayan Region) using Molecular Markers

Research Journal of Biotechnology ◽

10.25303/1611rjbt147154 ◽

2021 ◽

Vol 16 (11) ◽

pp. 147-154

Author(s):

Anjali Uniyal ◽

Akhilesh Kumar ◽

Sweta Upadhyay ◽

Vijay Kumar ◽

Sanjay Gupta

Keyword(s):

Genetic Diversity ◽

Molecular Markers ◽

Fragment Length Polymorphism ◽

Dna Polymorphisms ◽

Inter Simple Sequence Repeats ◽

Pharmaceutical Industries ◽

Endangered Medicinal Herb ◽

Identification And Characterization ◽

Simple Sequence

The Rheum species are important medicinal plants that are facing extinction due to their unplanned development and overexploitation by pharmaceutical industries. DNA polymorphisms are not prone to environmental modifications, thus they are widely used for the identification and characterization of plants. The use of different molecular markers has enabled the researchers for the valuation of genetic variability and diversity in its natural zone of distribution. The conventional approach may take several years to yield this information. For the estimation of molecular and genetic variations in geographical zone of distribution, various molecular markers technique are available like RAPD (Randomly Amplified Polymorphic DNA), RFLP (Restriction fragment length polymorphism), ISSR (Inter-Simple Sequence Repeats), SSR and AFLP. The uses of different molecular markers for the study of genetic diversity have been discussed in the review.

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Genetic Characterization of Cleveland Bay Horse Breed

Diversity ◽

10.3390/d11100174 ◽

2019 ◽

Vol 11 (10) ◽

pp. 174 ◽

Cited By ~ 2

Author(s):

Khanshour ◽

Hempsey ◽

Juras ◽

Cothran

Keyword(s):

Genetic Diversity ◽

Genetic Variability ◽

Genetic Similarity ◽

Genetic Resource ◽

Genetic Characterization ◽

Genetic Relationships ◽

Similarity Measures ◽

Horse Breed ◽

The Usa

The Cleveland Bay (CB) is the United Kingdom’s oldest established horse breed. In this study we analyzed the genetic variability in CB horses and investigated its genetic relationships with other horse breeds. We examined the genetic variability among 90 CB horses sampled in the USA compared to a total of 3447 horses from 59 other breeds. Analysis of the genetic diversity and population structure was carried out using 15 microsatellite loci. We found that genetic diversity in CB horses was less than that for the majority of other tested breeds. The genetic similarity measures showed no direct relationship between the CB and Thoroughbred but suggested the Turkman horses (likely in the lineage of ancestors of the Thoroughbred) as a possible ancestor. Our findings reveal the genetic uniqueness of the CB breed and indicate its need to be preserved as a genetic resource.

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Uncovering Olive Biodiversity through Analysis of Floral and Fruiting Biology and Assessment of Genetic Diversity of 120 Italian Cultivars with Minor or Marginal Diffusion

Biology ◽

10.3390/biology8030062 ◽

2019 ◽

Vol 8 (3) ◽

pp. 62 ◽

Cited By ~ 1

Author(s):

Lombardo ◽

Fila ◽

Lombardo ◽

Epifani ◽

III ◽

...

Keyword(s):

Genetic Diversity ◽

Genetic Variability ◽

Fruit Set ◽

Climatic Conditions ◽

Considerable Variability ◽

Almost All ◽

First Time ◽

Homogeneous Data ◽

Modest Effect

The primary impetus behind this research was to provide a boost to the characterization of the Italian olive biodiversity by acquiring reliable and homogeneous data over the course of an eight-year trial on the floral and fruiting biology of 120 molecularly analyzed cultivars, most of which have either low or very low diffusion. The obtained data highlighted a considerable variability to almost all of the analyzed parameters, which given the uniformity of environment and crop management was indicative of a large genetic variability in the accessions under observation, as confirmed through the molecular analysis. Several cases of synonymy were reported for the first time, even among plants cultivated in different regions, whilst all of the varieties examined, with only one exception, showed very low percentages of self-fruit-set, indicating a need for the employment of suitable pollinator plants. Eventually, a fitted model allowed us to evaluate the clear effects of the thermal values on blossoming, particularly in the months of March and April, whereas the climatic conditions during the flowering time had only a modest effect on its duration.

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