Bauhinia variegata L. and Croton sparsiflorus L. against the Human Vector Mosquitoes

Background: Bauhinia variegata Linn. is a native plant of Asia and China. B. variegata is found in tropical regions of the world. It belongs to family Leguminosae. It is used for diarrhea, hemorrhoids, constipation, piles, edema, leprosy, wounds, tumors, etc. Objective: The objective of the present study was to perform extraction of B. variegata flower and isolation of active constituents from the extract. Materials and Methods: The ethanolic extraction of B. variegata flower was performed using the Soxhlet apparatus. The isolation of active constituents from the extract was performed using chromatographic techniques. In column chromatographic studies, n-hexane- [dichloromethane (DCM)] (2:8) was used as an eluting system and further purified through thin layer chromatography (TLC). Compound A and B were isolated through chromatographic techniques, then the molecular formula and characterization of these compounds were carried out with mass and infrared (IR) spectral analysis. Results and Discussion: The percentage yield of B. variegata ethanolic extract (BVE) was found to be 20.8% w/w. The different fractions were F1 having 12.5 grams with n-hexane, F2 (17.1 grams) with CH2Cl2, F3 (21.2 grams) with EtOAc, and F4 (13.4 grams) with EtOH. Compound A and B were isolated from the solvent fractions of n-hexane-DCM (2:8) and EtOAc-DCM (1:9), respectively. The compound A was characterized as 3-hydroxy-6-methoxy-2-phenyl-4H-chromen-4-one. The compound B was characterized as 3-hydroxy-6-methyl-2-phenyl-4H-chromen-4-one. Conclusion: Thus, B. variegata flowers possess active components that need to identify their biological activities.

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Histochemistry and immunolocalisation of glucokinin in antidiabetic plants used in traditional Mexican medicine

European Journal of Histochemistry ◽

10.4081/ejh.2017.2782 ◽

2017 ◽

Cited By ~ 1

Author(s):

Guillermo Laguna-Hernández ◽

Carlos A. Rio-Zamorano ◽

Itzel G. Meneses-Ochoa ◽

Alicia E. Brechú-Franco

Keyword(s):

Medicinal Plants ◽

Public Health Problem ◽

Direct Application ◽

Glucosidase Inhibitors ◽

Naphthol Blue Black ◽

Bauhinia Variegata ◽

Alpha Glucosidase ◽

Antidiabetic Plants

Mexico is a megadiverse country that has 3,600 to 4,000 species of medicinal plants, of which approximately 800 are used to treat conditions related to diabetes mellitus (DM). DM is a chronic degenerative disease of energy metabolism that exists as two types: type 1 (DM1) and type 2 (DM2). DM is considered a public health problem that affects 7% of the Mexican population older than 20 years. DM is clinically controlled with hypoglycaemic drugs, alpha-glucosidase inhibitors, insulin secretion stimulants or the direct application of insulin. The hypoglycaemic effectiveness of specific molecules has been determined only for some medicinal plants in Mexico used to treat DM2. The presence of molecules called glucokinins, wich are similar to animal insulin molecules, has been reported in some plant species; glucokinins act as both growth factors and regulators of glucose metabolism in plants. Therefore, we hypothesized that the hypoglycaemic effectiveness of some of the popularly used species for the control of DM could be due to the presence of glucokinin, as reported for Bauhinia variegata. The goal of this work was to use histochemistry to detect, the accumulation of protein that is immunocytochemically compatible with glucokinin in slide sections of hypoglycaemic species used as remedies for DM2. The top fourteen most used medicinal plants in Mexico were selected for study via microscopic sections. Proteins were histochemically detected using naphthol blue black and Johansenâ€™s quadruple stain, and the immunocytochemical correspondence of the proteins with glucokinin was investigated using an insulin antibody. All species studied reacted positively to proteins and glucokinin in the same structures. The presence of glucokinin in these structures and the corresponding hypoglycaemic effects are discussed in the contex of the actions of other compounds.

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Protective Effect of Bauhinia variegata Leaf Extracts Against Oxidative Damage, Cell Proliferation and Bacterial Growth

Proceedings of the National Academy of Sciences India Section B Biological Sciences ◽

10.1007/s40011-015-0578-x ◽

2015 ◽

Vol 87 (1) ◽

pp. 45-51 ◽

Cited By ~ 7

Author(s):

Amit Kumar Sharma ◽

Uma Kant Sharma ◽

Abhay K. Pandey

Keyword(s):

Cell Proliferation ◽

Oxidative Damage ◽

Protective Effect ◽

Bacterial Growth ◽

Leaf Extracts ◽

Damage Cell ◽

Bauhinia Variegata

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Evaluation of Antinociceptive and Anti-Inflammatory Activities of a New Triterpene Saponin from Bauhinia variegata Leaves

Zeitschrift für Naturforschung C ◽

10.1515/znc-2009-11-1208 ◽

2009 ◽

Vol 64 (11-12) ◽

pp. 798-808 ◽

Cited By ~ 12

Author(s):

Mona A. Mohamed ◽

Madeha R. Mammoud ◽

Heiko Hayen

Keyword(s):

High Resolution ◽

Spectral Data ◽

Cinnamic Acid ◽

Cinnamic Acid Derivatives ◽

Triterpene Saponin ◽

H Nmr ◽

Antischistosomal Activity ◽

Bauhinia Variegata ◽

Anti Inflammatory ◽

C Nmr

A new triterpene saponin, named as 23-hydroxy-3α-[O-α-L-1C4-rhamnopyranosyl-(1´´4´)- O-α-L-4C1-arabinopyranosyl-oxy]olean-12-en-28-oic acid O-α-L-1C4-rhamnopyranosyl- (1´´´´´→4´´´´)-O-β-D-4C1-glucopyranosyl-(1´´´´→6´´´)-O-β-D-4C1-glucopyranosyl ester (9), was isolated from the leaves of Bauhinia variegata Linn. In addition, six flavonoid compounds along with two cinnamic acid derivatives were isolated and identified based on their chromatographic properties, and chemical and spectral data (ESI-high resolution-MSn, 1H NMR, 13C NMR, 1H-1H COSY, HSQC, and HMBC). Compound 9 was found to be nontoxic (LD50) and to have significant anti-inflammatory and antinociceptive activities. It also showed a slight antischistosomal activity.

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