scholarly journals Identification and Functional Characterization of Xanthomonas oryzae Pv. oryzae Isolates

2020 ◽  
pp. 78-84
Author(s):  
Diksha Kumari ◽  
Bishun Deo Parasad ◽  
Sangita Sahni ◽  
Abhijeet Ghatak
Keyword(s):  
Antioxidant Enzymes ◽  
Virulent Strain ◽  
Functional Characterization ◽  
Xanthomonas Oryzae ◽  
Host Pathogen Interaction ◽  
Ncbi Accession ◽  
Host Pathogen ◽  
Rice Leaves ◽  
Increased Activity

Rice is a model crop for studying host - pathogen interaction with one of the most devastating pathogens viz. Xanthomonas oryzae pv. oryzae (Xoo). In the present investigation, an attempt was made to isolate a virulent strain of Xathomonas oryzae from infected rice leaves and production of antioxidant enzymes, which are widely used in studying host - pathogen interactions. Among five isolates of X. oryzae pv. oryzae, SboBLB3 showed greater virulence as it showed susceptibility symptoms in infected rice leaves. The NCBI accession number of SboBLB3 was MH986180, which was obtained by sequencing 16s rDNA. The increased activity of antioxidant enzymes after SboBLB3 further confirms its virulence. Induction of antioxidant enzymes showed that SboBLB3 is a virulent strain of X. oryzae and can be used in host - pathogen interaction at molecular level.

scholarly journals Functional characterization of the collagen-binding protein DIP2093 and its influence on host–pathogen interaction and arthritogenic potential of Corynebacterium diphtheriae

Microbiology ◽  
2017 ◽  
Vol 163 (5) ◽  
pp. 692-701 ◽  
Author(s):  
Renata Stavracakis Peixoto ◽  
Camila Azevedo Antunes ◽  
Liliane Simpson Lourêdo ◽  
Vanilda Gonçalves Viana ◽  
Cintia Silva dos Santos ◽  
...  
Keyword(s):  
Binding Protein ◽  
Functional Characterization ◽  
Corynebacterium Diphtheriae ◽  
Collagen Binding ◽  
Host Pathogen Interaction ◽  
Host Pathogen

Functional characterization of the antioxidant enzymes in rice plants exposed to salinity stress

2017 ◽  
Vol 61 (3) ◽  
pp. 540-550 ◽  
Author(s):  
I. L. Vighi ◽  
L. C. Benitez ◽  
M. N. Amaral ◽  
G. P. Moraes ◽  
P. A. Auler ◽  
...  
Keyword(s):  
Antioxidant Enzymes ◽  
Salinity Stress ◽  
Functional Characterization ◽  
Rice Plants

Proteomic Characterization of a Natural Host–Pathogen Interaction: Repertoire of in Vivo Expressed Bacterial and Host Surface-Associated Proteins

2014 ◽  
Vol 14 (1) ◽  
pp. 120-132 ◽  
Author(s):  
Megan A. Rees ◽  
Oded Kleifeld ◽  
Paul K. Crellin ◽  
Bosco Ho ◽  
Timothy P. Stinear ◽  
...  
Keyword(s):  
Natural Host ◽  
Host Pathogen Interaction ◽  
Host Pathogen ◽  
Associated Proteins

Basics of pertussis pathogenesis

Pertussis ◽  
2018 ◽  
pp. 26-41 ◽  
Author(s):  
Amanda L. Skarlupka ◽  
Bodo Linz ◽  
Jennifer Maynard ◽  
Eric T. Harvill
Keyword(s):  
Animal Models ◽  
Adenylate Cyclase ◽  
Pertussis Toxin ◽  
Functional Characterization ◽  
Host Pathogen Interactions ◽  
Host Pathogen ◽  
Complex Picture ◽  
Molecular Components

The use of animal models and in vitro assays has allowed the identification and functional characterization of an iconic set of Bordetella pertussis factors that contribute to its pathogenesis. Much research on B. pertussis has been focused on the effects of pertussis toxin and adenylate cyclase toxin on pathogenesis and disease progression, and on the function of adherence factors. However, much larger sets of factors have been identified and proposed to be involved in host–pathogen interactions, including B. pertussis manipulating the host’s metabolism to its advantage. The identification of a third player, the resident microbiota, reveals a complex picture that has yet to be investigated. The ongoing studies of the molecular components produced by B. pertussis and their effect on the host during infection, commonly referred to as ‘pathogenesis’ research, are crucial to generate understanding of the host–pathogen interactions necessary to design new approaches to prevent and cure disease.

scholarly journals Role of the FeoB Protein and Siderophore in Promoting Virulence of Xanthomonas oryzae pv. oryzae on Rice

2010 ◽  
Vol 192 (12) ◽  
pp. 3187-3203 ◽  
Author(s):  
Alok Pandey ◽  
Ramesh V. Sonti
Keyword(s):  
Gene Cluster ◽  
Functional Characterization ◽  
Biosynthetic Gene Cluster ◽  
Xanthomonas Oryzae ◽  
In Planta ◽  
Growth Deficiency ◽  
Constitutive Production ◽  
Serious Disease

ABSTRACT Xanthomonas oryzae pv. oryzae causes bacterial blight, a serious disease of rice. Our analysis revealed that the X. oryzae pv. oryzae genome encodes genes responsible for iron uptake through FeoB (homolog of the major bacterial ferrous iron transporter) and a siderophore. A mutation in the X. oryzae pv. oryzae feoB gene causes severe virulence deficiency, growth deficiency in iron-limiting medium, and constitutive production of a siderophore. We identified an iron regulated xss gene cluster, in which xssABCDE ( X anthomonas siderophore synthesis) and xsuA ( X anthomonas siderophore utilization) genes encode proteins involved in biosynthesis and utilization of X. oryzae pv. oryzae siderophore. Mutations in the xssA, xssB, and xssE genes cause siderophore deficiency and growth restriction under iron-limiting conditions but are virulence proficient. An xsuA mutant displayed impairment in utilization of native siderophore, suggesting that XsuA acts as a specific receptor for a ferric-siderophore complex. Histochemical and fluorimetric assays with gusA fusions indicate that, during in planta growth, the feoB gene is expressed and that the xss operon is not expressed. This study represents the first report describing a role for feoB in virulence of any plant-pathogenic bacterium and the first functional characterization of a siderophore-biosynthetic gene cluster in any xanthomonad.

scholarly journals Identification and functional characterization of small non-coding RNAs in Xanthomonas oryzae pathovar oryzae

BMC Genomics ◽  
2011 ◽  
Vol 12 (1) ◽  
Author(s):  
Hong Liang ◽  
Ying-Tao Zhao ◽  
Jie-Qiong Zhang ◽  
Xiu-Jie Wang ◽  
Rong-Xiang Fang ◽  
...  
Keyword(s):  
Functional Characterization ◽  
Xanthomonas Oryzae ◽  
Non Coding Rnas

scholarly journals Molecular Cloning and Functional Characterization of Heat Stress-Responsive Superoxide Dismutases in Garlic (Allium sativum L.)

Antioxidants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 815
Author(s):  
Hyo Seong Ji ◽  
Seoung Gun Bang ◽  
Min-A Ahn ◽  
Gayeon Kim ◽  
Eunhui Kim ◽  
...  
Keyword(s):  
Antioxidant Enzymes ◽  
Heat Stress ◽  
Molecular Cloning ◽  
Allium Sativum ◽  
Functional Characterization ◽  
Superoxide Dismutases ◽  
Sod Activity ◽  
Depth Analysis ◽  
Solid Foundation

Superoxide dismutases (SODs) are key antioxidant enzymes that can detoxify the superoxide radicals generated by various stresses. Although various plant SODs have been suggested to improve stress tolerance, SODs in garlic, an economically important vegetable grown worldwide, remain relatively unknown. In this study, we found that heat stress strongly induced the activities of Cu/ZnSODs, FeSODs, and MnSODs in garlic leaves. In addition, we cloned four garlic SODs (AsSODs) and suggest that heat stress-increased SOD activity was reflected at least by the induction of these AsSODs. The results of the agro-infiltration assay suggested that the cloned AsSODs encoded functional SOD enzymes belonging to the Cu/ZnSOD and MnSOD families. As a first step toward understanding the enzymatic antioxidant system in garlic plants, our results provide a solid foundation for an in-depth analysis of the physiological functions of the AsSOD family.

scholarly journals Insights Into the Roles of Two Genes of the Histidine Biosynthesis Operon in Pathogenicity of Xanthomonas oryzae pv. oryzicola

2018 ◽  
Vol 108 (5) ◽  
pp. 542-551 ◽  
Author(s):  
Panpan Su ◽  
Zhiwei Song ◽  
Guichun Wu ◽  
Yancun Zhao ◽  
Yuqiang Zhang ◽  
...  
Keyword(s):  
Functional Characterization ◽  
Shikimate Pathway ◽  
Xanthomonas Oryzae ◽  
Bifunctional Enzyme ◽  
Transcriptional Expression ◽  
Bacterial Leaf Streak ◽  
In Planta ◽  
Histidine Biosynthesis ◽  
Tobacco Plants

Xanthomonas oryzae pv. oryzicola is an X. oryzae pathovar that causes bacterial leaf streak in rice. In this study, we performed functional characterization of a nine-gene his operon in X. oryzae pv. oryzicola. Sequence analysis indicates that this operon is highly conserved in Xanthomonas spp. Auxotrophic assays confirmed that the his operon was involved in histidine biosynthesis. We found that two genes within this operon, trpR and hisB, were required for virulence and bacterial growth in planta. Further research revealed that trpR and hisB play different roles in X. oryzae pv. oryzicola. The trpR acts as a transcriptional repressor and could negatively regulate the expression of hisG, -D, -C, -B, -H, -A, and -F. hisB, which encodes a bifunctional enzyme implicated in histidine biosynthesis, was shown to be required for xanthomonadin production in X. oryzae pv. oryzicola. The disruption of hisB reduced the transcriptional expression of five known shikimate pathway-related genes xanB2, aroE, aroA, aroC, and aroK. We found that the his operon in X. oryzae pv. oryzicola is not involved in hypersensitive response in nonhost tobacco plants. Collectively, our results revealed that two genes in histidine biosynthesis operon play an important role in the pathogenicity of X. oryzae pv. oryzicola Rs105.

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