Acute Toxicity Study and Ascertaination of Wound Healing Effect of the Acetone Fraction of Tetrapleura tetraptera Fruit in Excision Wound Model

Objectives. The present study was aimed at investigating the wound healing effect of ethanolic extract of Cestrum nocturnum (L.) leaves (EECN) using excision and incision wound model. Methods. Wistar albino rats were divided into five groups each consisting of six animals; group I (left untreated) considered as control, group II (ointment base treated) considered as negative control, group III treated with 5% (w/w) povidone iodine ointment (Intadine USP), which served as standard, group IV treated with EECN 2% (w/w) ointment, and group V treated with EECN 5% (w/w) ointment were considered as test groups. All the treatments were given once daily. The wound healing effect was assessed by percentage wound contraction, epithelialization period, and histoarchitecture studies in excision wound model while breaking strength and hydroxyproline content in the incision wound model. Result. Different concentration of EECN (2% and 5% w/w) ointment promoted the wound healing activity significantly in both the models studied. The high rate of wound contraction (P<0.001), decrease in the period for epithelialization (P<0.01), high skin breaking strength (P<0.001), and elevated hydroxyproline content were observed in animal treated with EECN ointments when compared to the control and negative control group of animals. Histopathological studies of the EECN ointments treated groups also revealed the effectiveness in improved wound healing. Conclusions. Ethanolic extract of Cestrum nocturnum (EECN) leaves possesses a concentration dependent wound healing effect.

Download Full-text

Comparative study of wound healing effect of topical Acacia catechu extract and silver sulfadiazine on excisional wound model in guinea pigs

International Journal of Basic & Clinical Pharmacology ◽

10.18203/2319-2003.ijbcp20184844 ◽

2018 ◽

Vol 7 (12) ◽

pp. 2347

Author(s):

Sunitha Tangeti ◽

Padmaja Gabbita ◽

Raghunatha R. Ponnaluri ◽

Bhanu P. Kolasani

Keyword(s):

Wound Healing ◽

Guinea Pigs ◽

Silver Sulfadiazine ◽

P Value ◽

Healing Effect ◽

Wound Model ◽

Excisional Wound ◽

Wound Healing Effect ◽

The Mean ◽

Acacia Catechu

Background: Impaired and aberrant wound healing imposes a huge financial burden and places an enormous drain in health care resources in the developed world and an insurmountable problem in the developing countries too. In this study, authors have compared the wound healing effect of topical application of Acacia catechu extract with silver sulfadiazine in excisional wound model in guinea pigs.Methods: Twelve guinea pigs were randomly divided into two groups (n=6). The wound healing was observed in excision model. The standard group is treated with silver sulfadiazine (group 1) and the test group with Acacia catechu extract (group 2). The mean wound size expressed in mm2 and the mean percentage of the wound healed was measured on day 0, 4, 8, 12, 16 and 19. Biopsy was done on day 21 for histopathological examination (HPE).Results: Statistical analysis was done by using unpaired t test for between the groups comparison and by using paired t test for within the group comparison. The mean percentage of the wound healed with-in the groups was found to be statistically highly significant (p value <0.001) and in between the groups was found to be statistically non-significant (p value >0.05). A comparable increase in collagen content and granulation tissue was found on HPE in both the groups on day 21.Conclusions: Wound healing effect of the Acacia catechu extract is equal to and comparable with that of silver sulfadiazine.

Download Full-text

Evaluation of the Wound Healing Potential ofAchillea biebersteiniiAfan. (Asteraceae) byIn VivoExcision and Incision Models

Evidence-based Complementary and Alternative Medicine ◽

10.1093/ecam/nep039 ◽

2011 ◽

Vol 2011 ◽

pp. 1-7 ◽

Cited By ~ 24

Author(s):

Esra Küpeli Akkol ◽

Ufuk Koca ◽

Ipek Pesin ◽

Demet Yilmazer

Keyword(s):

Wound Healing ◽

Folk Medicine ◽

Reference Drug ◽

Excision Wound ◽

Wound Model ◽

Achillea Biebersteinii ◽

Healing Of Wounds ◽

Wound Healing Effect ◽

Incision Wound ◽

Wound Healing Activity

Achilleaspecies are widely used for diarrhea, abdominal pain, stomachache and healing of wounds in folk medicine. To evaluate the wound healing activity of the plant, extracts were prepared with different solvents; hexane, chloroform, ethyl acetate and methanol, respectively from the roots ofAchillea biebersteinii. Linear incision by using tensiometer and circular excision wound models were employed on mice and rats. The wound healing effect was comparatively evaluated with the standard skin ointment Madecassol. Then-hexane extract treated groups of animals showed 84.2% contraction, which was close to contraction value of the reference drug Madecassol (100%). On the other hand the same extract on incision wound model demonstrated a significant increase (40.1%) in wound tensile strength as compared to other groups. The results of histoptological examination supported the outcome of linear incision and circular excision wound models as well. The experimental data demonstrated thatA. biebersteiniidisplayed remarkable wound healing activity.

Download Full-text

Wound Healing Potential of Crocin and Safranal, Main Saffron (Crocus sativus L.), the Active Constituents in Excision Wound Model in Rats

Galen Medical Journal ◽

10.31661/gmj.v10i0.1900 ◽

2021 ◽

Vol 10 ◽

Author(s):

Nafise Deldar ◽

Malihezaman Monsefi ◽

Mohsen Salmanpour ◽

Mohadeseh Ostovar ◽

Mojtaba Heydari

Keyword(s):

Wound Healing ◽

Crocus Sativus ◽

Control Group ◽

Topical Formulation ◽

Adult Rats ◽

Wound Area ◽

Active Constituents ◽

Healing Effect ◽

Wound Model ◽

Wound Healing Effect

Background: Saffron is traditionally suggested for wound healing in Persian medicine. It is investigated for wound healing effect in multiple studies with promising results. It is not examined that which ingredient of saffron contributes more to this effect. This study was aimed to evaluate and compare the wound healing potential of saffron and its active constituents, crocin, and safranal, in rats. Materials and Methods: Forty female adult rats with induced excision wounds were randomly divided into four groups to receive topical formulation of saffron, crocin, safranal, and placebo for seven days. The Wound area and histopathologic stage of wound healing were evaluated as outcome measures. Results: The wound area was significantly lower in treatment groups (saffron, crocin, and safranal) compared to the control group on day 7 of the intervention. Compared to the control group, the wound in all treated groups showed a decreased inflammatory response and more progression to the proliferation phase. The saffron group showed more advanced healing phase with complete epithelialization of the wound on day 7 of study when partial and no epithelialization was observed in safranal and crocin groups. Conclusion: The study showed the wound healing properties of both safranal and crocin with the slight superiority of safranal. However, saffron seems to be more potent in wound healing effect compared to safranal and crocin as its main active constituents. [GMJ.2021;10:e1900]

Download Full-text

DEVELOPMENT OF HERBOMINERAL FORMULATION FROM DAUCUS CAROTA (LINN) FOR WOUND HEALING EFFECTS

INDIAN DRUGS ◽

10.53879/id.58.07.12236 ◽

2021 ◽

Vol 58 (07) ◽

pp. 66-68

Author(s):

Rekha Tarasingh Rajput ◽

Kashmira J. Gohil ◽

Sanjeet Kumar ◽

Keyword(s):

Wound Healing ◽

Breaking Strength ◽

Positive Control ◽

Control Group ◽

Standard Group ◽

Time Intervals ◽

Healing Effect ◽

Wound Model ◽

Wound Healing Effect ◽

Incision Wound

A herbomineral formulation was developed in ointment form by using herb extract, mineral and other excipients and evaluated for wound healing effect in excision and incision wound models in rats. Excision wounds sized 500 mm2 and 2 mm depth were used to study the rate of contraction of wound and epithelialization at different time intervals. Incision wounds were used for the study of tensile strength measured on the 10th day. It was observed that the herbomineral formulation in the excision wound model possessed a significant (p<0.001) wound healing effect compared to a positive control group and was nearly comparable with a standard group. In incision wounds, the formulation showed a significant increase in the skin breaking strength compared to control and reference standards.The wound healing effect of the formulation may be attributed to phytoconstituents like flavonoid and phenolic derivatives present in the root extract.The herbomineral ointment was also evaluated for various pharmaceutical parameters and was found to be stable for 45 days.

Download Full-text

Evaluation of the wound-healing effect of an N-isopropenylimidazole zinc metal complex derivative on a linear skin wound model in rats

Farmaciya (Pharmacy) ◽

10.29296/25419218-2021-06-09 ◽

2021 ◽

Vol 70 (6) ◽

pp. 49-56

Author(s):

S. A. Lebedeva ◽

P.A. Galenko-Yaroshevsky (Jr.) ◽

S.I. Melnik ◽

S.V. Kozin ◽

T. A. Demura ◽

...

Keyword(s):

Wound Healing ◽

Metal Complex ◽

Skin Wound ◽

Zinc Metal ◽

Healing Effect ◽

Wound Model ◽

Wound Healing Effect ◽

Complex Derivative

Download Full-text

In vivo investigation of the wound healing effect of the traditional Hungarian medicinal plant Centaurea sadleriana

Planta Medica ◽

10.1055/s-0029-1234390 ◽

2009 ◽

Vol 75 (09) ◽

Cited By ~ 1

Author(s):

D Csupor ◽

G Blazsó ◽

Á Balogh ◽

J Hohmann

Keyword(s):

Wound Healing ◽

Medicinal Plant ◽

Healing Effect ◽

Wound Healing Effect

Download Full-text

The diabetic wound healing effect of a two-herb formula and its mechanisms of action

Planta Medica ◽

10.1055/s-0032-1320306 ◽

2012 ◽

Vol 78 (11) ◽

Author(s):

CBS Lau ◽

VKM Lau ◽

CL Liu ◽

PKK Lai ◽

JCW Tam ◽

...

Keyword(s):

Wound Healing ◽

Mechanisms Of Action ◽

Diabetic Wound ◽

Healing Effect ◽

Diabetic Wound Healing ◽

Wound Healing Effect

Download Full-text

PHARMACOGNOSTIC, ANTIOXIDANT AND ACUTE TOXICITY STUDY OF Ficus sycomorus (Linn) (Moraceae) ROOT AND STEM BARK

FUDMA Journal of Sciences ◽

10.33003/fjs-2020-0402-244 ◽

2020 ◽

Vol 4 (2) ◽

pp. 605-614

Author(s):

Murtala M. Namadina ◽

H. Haruna ◽

U. Sanusi

Keyword(s):

Acute Toxicity ◽

Radical Scavenging ◽

Stem Bark ◽

The Body ◽

Toxicity Study ◽

Bark Extract ◽

Root Extract ◽

Acute Toxicity Study ◽

Radical Scavenging Ability ◽

Phytochemical Components

Most of biochemical reactions in the body generates Reactive Oxygen Species (ROS), which are involved in the pathogenesis of oxidative stress-related disorders like diabetes, nephrotoxicity, cancer, cardiovascular disorders, inflammation and neurological disorders when they attack biochemical molecules like proteins, lipids and nucleic acid. Antioxidants are used to protect the cells or tissues against potential attack by ROS. Most medicinal plants possess a rich source of antioxidants such as flavonoids, phenols, tannins, alkaloids among others. These phytochemicals are currently pursued as an alternative and complimentary drug. In this study, phytochemical components, antioxidant and acute toxicity study of the methanol extract of stem bark and root of F. sycomorus were carried out using standard methods. Findings from this study revealed the presence of some diagnostic microscopical features such as calcium oxalate, starch, gum/mucilage, lignin, Aleurone grain, suberized/Cuticular cell wall and inulin but calcium carbonate was absent in stem bark but present in the powdered root. Quantitative physical constants include moisture contents (6.40% and 7.82%), ash value (7.20% and 9.30 %) in stem bark and root respectively. Carbohydrates, alkaloid, flavonoids, saponins, tannins, glycoside, steroid, triterpenes and phenols were present in all the extracts. They were found to exhibit potent 1,1,-diphenyl 2-picryl hydrazyl (DPPH) free scavenging activity. The DPPH radical scavenging ability of the extracts showed the following trend Ascorbic acid < stem bark extract˃ root extract. The LD50 of the methanolic stem bark and root extracts were found to be greater than 5000 mg /kg and is considered safe for use. Nonetheless, further

Download Full-text

In-vitro and in-vivo evaluation of antidiabetic activity of Withania coagulans extract

The Natural Products Journal ◽

10.2174/2210315509666190416155757 ◽

2019 ◽

Vol 09 ◽

Author(s):

Tejas Patel ◽

B.N. Suhagia

Keyword(s):

Blood Glucose ◽

Acute Toxicity ◽

Aqueous Extract ◽

Pancreatic Beta Cell ◽

Toxicity Study ◽

Acute Toxicity Study ◽

Withania Coagulans ◽

Study Results

Background: Diabetes mellitus is major issue to public health as its prevalence is rising day by day. Synthetic agents available for the diabetic treatment are expensive or produce undesirable side effect on chronic use and some of them are not suitable during pregnancy. Herbal medicines accepted widely due to side effects and low cost. Objective: The aim of present study was to evaluate the activity of Withania coagulans extract using In-vitro and In-vivo model. Methods: Different three types of Withania coagulans extract were prepared using aqueous (W1), Alcohol (W2) and hydro-alcoholic (50:50) mixture (W3). In-vitro Anti-diabetic activity of the all three extracts evaluated using RINm5F Pancreatic beta cells.Further, n-vivo anti-diabetic evaluation performed by administering 50 mg/kg (p.o) aqueous extract for 7 days in Streptozotocin (STZ)-induced mice. Body weight of the animals was also determined to perform acute toxicity study. Results: The results of in –vitro cell based study indicated that among all three extract, aqueous extract (W1) of Withania coagulans showed potential increase in inulin release. The EC50 of the W1 (249.6 µg/L) which is compared with standard (Glibenclamide) EC50. From the results of In-vitro study, W1 subjected for acute toxicity study and the acute toxicity study results indicated LD50 of 50mg/kg. Diabetic rats treated with W1 extract at oral dose of 50 mg/kg for 7 days showed 34.17% reduction in blood glucose in comparison to untreated diabetic (STZ-induced) rats. Blood glucose levels of Standard treated (Glibenclamide) and control untreated. Conclusion: In conclusion, results of pancreatic beta cell based study showed increase in insulin release by administration of extract. Further aqueous extract (W1) was potentially reduced blood glucose level in STZ induced diabetic mice.

Download Full-text