Pharmacological Investigation of the Wound Healing Activity of Cestrum nocturnum (L.) Ointment in Wistar Albino Rats

Objectives. The present study was aimed at investigating the wound healing effect of ethanolic extract of Cestrum nocturnum (L.) leaves (EECN) using excision and incision wound model. Methods. Wistar albino rats were divided into five groups each consisting of six animals; group I (left untreated) considered as control, group II (ointment base treated) considered as negative control, group III treated with 5% (w/w) povidone iodine ointment (Intadine USP), which served as standard, group IV treated with EECN 2% (w/w) ointment, and group V treated with EECN 5% (w/w) ointment were considered as test groups. All the treatments were given once daily. The wound healing effect was assessed by percentage wound contraction, epithelialization period, and histoarchitecture studies in excision wound model while breaking strength and hydroxyproline content in the incision wound model. Result. Different concentration of EECN (2% and 5% w/w) ointment promoted the wound healing activity significantly in both the models studied. The high rate of wound contraction (P<0.001), decrease in the period for epithelialization (P<0.01), high skin breaking strength (P<0.001), and elevated hydroxyproline content were observed in animal treated with EECN ointments when compared to the control and negative control group of animals. Histopathological studies of the EECN ointments treated groups also revealed the effectiveness in improved wound healing. Conclusions. Ethanolic extract of Cestrum nocturnum (EECN) leaves possesses a concentration dependent wound healing effect.

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Evaluation of the Wound Healing Activity of the Crude Extract of Leaves of Acanthus polystachyus Delile (Acanthaceae)

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2018/2047896 ◽

2018 ◽

Vol 2018 ◽

pp. 1-9 ◽

Cited By ~ 4

Author(s):

Wubante Demilew ◽

Getnet Mequanint Adinew ◽

Seyfe Asrade

Keyword(s):

Wound Healing ◽

Medicinal Plants ◽

Crude Extract ◽

Negative Control Group ◽

Treated Group ◽

Negative Control ◽

Control Group ◽

Wound Model ◽

Treatment Of Wounds ◽

Wound Healing Activity

Background. Medicinal plants play indispensable roles to treat various ailments. Acanthus polystachyus is one of the medicinal plants used traditionally for treatment of wounds. However, there were no scientific reports documented so far on the wound healing activities of this plant. Thus, the present study provides a scientific evaluation for the wound healing potential of the crude extract of A. polystachyus leaves. Methods. The crude extraction was carried out using 80% methanol. The crude extract was prepared in 5% (w/w) and 10% (w/w) ointment and evaluated for wound healing activity using excision, infected, and incision wound models in Swiss albino mice. Results. Both 5% and 10% (w/w) ointments significantly reduced period of epithelialization and increased wound contraction rate and tensile strength compared to the negative control group (P < 0.05). The wound healing activity of 10% (w/w) ointment treated group was greater than 5% (w/w) and nitrofurazone ointment treated groups in S. aureus infected wound model. Conclusion. These results demonstrate that the crude extract of A. polystachyus leaves possesses wound healing activities. This justifies the traditional claimed use of the plant for treating uninfected and infected wounds caused by S. aureus.

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Preliminary Study on Wound Healing Activity of Propolis in Albino Rats

International Journal of Innovative Research in Medical Science ◽

10.23958/ijirms/vol01-i07/01 ◽

2016 ◽

Vol 1 (07) ◽

Author(s):

A.Timucin ATAYOGLU ◽

Sibel SILICI

Keyword(s):

Wound Healing ◽

Treated Group ◽

Control Group ◽

Albino Rats ◽

Wound Model ◽

In Vitro Antibacterial Activity ◽

Group 3 ◽

Group 2 ◽

Wistar Albino Rats

Background: Infection can lead to delayed wound healing. Recently it has been shown that propolis which is used in complementary medicine has an antibacterial and anti-inflammatory effect. The aim of this study is to determine whether propolis may contribute to wound healing. Material and Methods: Twenty-one male Wistar albino rats were randomly divided into three groups. Group1 and Group 2 were topically treated with propolis ointment and Thiocillin® oinment, respectively while Group 3 was the control group. On incision wound model, Thiocillin® and propolis ointments were applied on wound sites once daily for 30 days and the mean epidermal thickness (MET) at the 30th day was compared while antimicrobial activity of propolis was studied against different pathogens as well. Results: Propolis exhibited in vitro antibacterial activity against Staphylococcus aureus, Escherichia coli, Streptococcus sp. and Pseudomonas sp. It is observed that the MET in the groups of Propolis ointment and Thiocillin® ointment were significantly greater than that of the control group, while the MET in the group of propolis ointment was significantly greater than that of Thiocillin® ointment treated group. Conclusion: Propolis is effective in wound healing. Further study in-depth is necessary to probe into clinical correlation.

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Is traditional herbal mix composition effective on wound healing?

Medical Science and Discovery ◽

10.36472/msd.v8i1.455 ◽

2021 ◽

Vol 8 (1) ◽

pp. 29-38

Author(s):

Serap Gokce Eskin ◽

Yucel Basimoglu Koca ◽

Serdal Ogut

Keyword(s):

Wound Healing ◽

Negative Control Group ◽

Grape Seed ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Sprague Dawley ◽

Wound Model ◽

Experimental Group

Objective: Wound healing is a dynamic process that includes biochemical and physiological phenomena. Studies confirm that plants are used in wound healing to a great extent. Antique oil (HBX 2371) has been produced as a traditional herbal mixed. This herbal mixture contains sesame oil, thyme oil, olive leaf, fig seed, grape seed, turmeric, and cinnamon. We evaluated traditional plant mixed oil ( Antique oil) for its wound-healing activity using an excision wound model in rats. Methods: The study used a preclinical, in vivo experimental, and analytical design. In the study, a total of 21 Sprague-Dawley rats, including 7 as experimental group, 7 as negative-control group, and 7 as positive-control group, were used. After the ischemic wound was created in experimental animals, the research data relating to the histochemical changes and biochemical parameters of the wound healing parameters were collected Results: A to research in experimental group fibroblast count, collagen density, fat cells, epithelization scores higher than the other groups and inflammatory cell density lower than the others. According to the results of the study, the highest TAC value (3.94 ± 0.21) was determined in the experimental group and results showed that antique oil administration decreased the TOC value. Conclusion: In conclusion, it was observed that antique oil prevented the wound changes induced by the incision, increased the repair of the epidermal and dermal structure in a short time, increased the antioxidant level, and decreased the oxidation level. Clinical studies are recommended.

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Wound Healing Potential of Crocin and Safranal, Main Saffron (Crocus sativus L.), the Active Constituents in Excision Wound Model in Rats

Galen Medical Journal ◽

10.31661/gmj.v10i0.1900 ◽

2021 ◽

Vol 10 ◽

Author(s):

Nafise Deldar ◽

Malihezaman Monsefi ◽

Mohsen Salmanpour ◽

Mohadeseh Ostovar ◽

Mojtaba Heydari

Keyword(s):

Wound Healing ◽

Crocus Sativus ◽

Control Group ◽

Topical Formulation ◽

Adult Rats ◽

Wound Area ◽

Active Constituents ◽

Healing Effect ◽

Wound Model ◽

Wound Healing Effect

Background: Saffron is traditionally suggested for wound healing in Persian medicine. It is investigated for wound healing effect in multiple studies with promising results. It is not examined that which ingredient of saffron contributes more to this effect. This study was aimed to evaluate and compare the wound healing potential of saffron and its active constituents, crocin, and safranal, in rats. Materials and Methods: Forty female adult rats with induced excision wounds were randomly divided into four groups to receive topical formulation of saffron, crocin, safranal, and placebo for seven days. The Wound area and histopathologic stage of wound healing were evaluated as outcome measures. Results: The wound area was significantly lower in treatment groups (saffron, crocin, and safranal) compared to the control group on day 7 of the intervention. Compared to the control group, the wound in all treated groups showed a decreased inflammatory response and more progression to the proliferation phase. The saffron group showed more advanced healing phase with complete epithelialization of the wound on day 7 of study when partial and no epithelialization was observed in safranal and crocin groups. Conclusion: The study showed the wound healing properties of both safranal and crocin with the slight superiority of safranal. However, saffron seems to be more potent in wound healing effect compared to safranal and crocin as its main active constituents. [GMJ.2021;10:e1900]

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DEVELOPMENT OF HERBOMINERAL FORMULATION FROM DAUCUS CAROTA (LINN) FOR WOUND HEALING EFFECTS

INDIAN DRUGS ◽

10.53879/id.58.07.12236 ◽

2021 ◽

Vol 58 (07) ◽

pp. 66-68

Author(s):

Rekha Tarasingh Rajput ◽

Kashmira J. Gohil ◽

Sanjeet Kumar ◽

Keyword(s):

Wound Healing ◽

Breaking Strength ◽

Positive Control ◽

Control Group ◽

Standard Group ◽

Time Intervals ◽

Healing Effect ◽

Wound Model ◽

Wound Healing Effect ◽

Incision Wound

A herbomineral formulation was developed in ointment form by using herb extract, mineral and other excipients and evaluated for wound healing effect in excision and incision wound models in rats. Excision wounds sized 500 mm2 and 2 mm depth were used to study the rate of contraction of wound and epithelialization at different time intervals. Incision wounds were used for the study of tensile strength measured on the 10th day. It was observed that the herbomineral formulation in the excision wound model possessed a significant (p<0.001) wound healing effect compared to a positive control group and was nearly comparable with a standard group. In incision wounds, the formulation showed a significant increase in the skin breaking strength compared to control and reference standards.The wound healing effect of the formulation may be attributed to phytoconstituents like flavonoid and phenolic derivatives present in the root extract.The herbomineral ointment was also evaluated for various pharmaceutical parameters and was found to be stable for 45 days.

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EVALUATION OF WOUND HEALING ACTIVITY OF METHANOLIC EXTRACT OF SMILAX WIGHTII (A. DC.) IN WISTAR ALBINO RATS

Kongunadu Research Journal ◽

10.26524/krj17 ◽

2014 ◽

Vol 1 (1) ◽

pp. 73-77

Author(s):

Uma Maheswari P ◽

Shalimol A ◽

Arumugasamy A ◽

Udhaya Sankar M.R

Keyword(s):

Wound Healing ◽

Dry Weight ◽

Wound Contraction ◽

Albino Rats ◽

Standard Drug ◽

Excision Wound ◽

Wound Model ◽

Wistar Albino Rats ◽

Incision Wound ◽

Wound Healing Activity

The present experimental study was designed to evaluate the wound healing activity of methanolic extract of Smilax wightii A. DC. on incision and excision wound models in Wistar albino rats. The parameters studied were wound breaking strength, wound contraction area, epithelialization period, granulation tissuewet, dry weight and hydroxyproline content in incision wound model, percentage of wound contraction and period of epithelialization in excision wound model. The rats were administered topically with 100mg/kg b.wt. (low dosage), 200mg/kg b.wt. (moderate dosage) and 500mg/kg b.wt. (high dosage) of methanolicextract of Smilax wightii (MESW). The activity of the extract treated groups were compared with that of the control 1% Spirit. Framycetin sulphate 0.2% w/w was used as the standard drug. In incision wound model, there was a significant increase in the wound breaking strength in all the experimental groups treated withMESW than that of the control. Similarly, significant (P<0.001) decrease in wound contraction area and period of epithelialization were also observed in the test group animals treated with MESW and the standard drug treated groups when compared to that of the control. , a significant increase was observed in granulation tissue wet and dry weight and hydroxyproline content in the test groups treated with MESW compared to the control. In exicision wound model, there was a significant increase (P<0.01) in the percentage of wound contraction and decrease in period of epithelialization in the experimental groups treated with 200mg/kgb.wt. (moderate dosage) and 500mg/kgb.wt (high dosage) of MESW. The extract treated groups showed significant improvement in all the wound healing parameters of incision, and excision wound models.

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Effects of 1% and 3% Mobe Leaf Extract Gel on Socket Wound Healing after Tooth Extraction

Dentika Dental Journal ◽

10.32734/dentika.v24i1.5327 ◽

2021 ◽

Vol 24 (1) ◽

pp. 1-5

Author(s):

Olivia Avriyanti Hanafiah ◽

Denny Satria ◽

Avi Syafitri

Keyword(s):

Wound Healing ◽

Tooth Extraction ◽

Leaf Extract ◽

Alveolar Bone ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

P Value ◽

Gel Treatment

Tooth extraction is a process of removing teeth from the alveolar bone. In wound healing, fibroblast are very important cells. The main purpose of this study was to determine the effect of mobe leaf 1% and 3% extract gel (Artocarpus lakoocha) on fibroblast proliferation in post extraction tooth socket wound healing. This research used 16 samples of wistar rats, divided into 4 groups, a positive control group, a negative control group and a 1% and 3% mobe leaf extract gel group. The left mandibular incisors were extracted, then 1% and 3% gels of mobe leaf extract were applied on day 1 to day 7. Data analysis was calculated using the Kruskal-Wallis test on clinical data and one way ANOVA test for microscopic. The result of the socket wound healing activity test for a good concentration of mobe leaf extract gel was 3%. This research shows significant resultith p-value of 0.018 (< 0.05) on the closure of the socket wound clinically which means the closure of the wound accelerates because of the mobe leaf 3% extract gel treatment. The distance of fibroblast on microscopically shows significant resultith a p-value of 0.002 (< 0.05), which means that there was an enlargement of the distance fibroblast at the socket wound closure with application of mobe leaf 3% extract gel. From the results of the study it can be concluded that mobe leaf 3% extract gel has the best ability to show acceleration the closure of the socket wound either clinically or microscopically.

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EVALUATION OF EXCISIONAL AND INCISIONAL WOUND HEALING ACTIVITY OF ELECTROHOMEOPATHIC DRUG (SPAGYRIC ESSENCE) GREEN ELECTRICITY IN RATS

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2020v12i10.38674 ◽

2020 ◽

pp. 72-75

Author(s):

PRASANT KUMAR SABAT ◽

SWETA PRIYADARSINI PRADHAN ◽

RANDEEP PATRO

Keyword(s):

Wound Healing ◽

Tensile Strength ◽

Wound Contraction ◽

Control Group ◽

Albino Rats ◽

Green Electricity ◽

Wound Model ◽

Wound Protection ◽

Incision Wound ◽

Wound Healing Activity

Objective: The current research is an attempt to evaluate the wound healing activity of Electrohomeopathic drug Green electricity (GE) in Wistar rats. No data have been reported till date on the wound healing effect of GE. Hence the present research focuses on the scientific investigation of the wound healing activity of GE in rats. Methods: Excision and incision wounds were inflicted upon albino rats. In the excision wound model, the wound contraction, Scar residue, area and time of complete epithelization were measured subsequently on day 0, 3,6,9,12,15, 18 and 21 after inflicting the wound. Wound contraction was calculated as a percentage change in the initial wound size. In the incision wound model, the tensile strength was measured on the 10th post wounding day. Results: It was noted that the effect produced by the GE Showed significant wound healing (p<0.01) in all wound models when compared to the control group. In the case of the excision model, GE statistically increases the wound contraction rate and in the case of the incision model GE showed high tensile strength as compared to the control group. In both cases the epithelization period was significantly decreased as compared to control. Almost 100% wound protection was achieved by GE in 21 d as compared to control. Conclusion: GE (ointment) showed significant wound healing activity (p<0.01) in both the excised wound model and incision wound model in rats.

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ASSESSMENT OF ANTI-INFLAMMATORY ACTIVITY OF ETHANOLIC EXTRACT OF ASAM KANDIS (GARCINIA XANTHOCHYMUS HOOK. F. EX T. ANDERSON) FRUIT

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11i4.23578 ◽

2018 ◽

Vol 11 (4) ◽

pp. 81

Author(s):

Hanafis Sastra Winata ◽

Rosidah Rosidah ◽

Panal Sitorus

Keyword(s):

Ethanolic Extract ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Inflammatory Activity ◽

Control Group ◽

Phytochemical Screening ◽

Fruit Extract ◽

Garcinia Xanthochymus ◽

Anti Inflammatory

Objective: The objective of this study was to evaluate the anti-inflammatory activity in acute and subacute models of inflammation from ethanolic fruit extract of Asam kandis (Garcinia xanthochymus Hook. f. ex T. Anderson) in animal (rats) models.Methods: Pleliminary phytochemical screening was carried out by using standard procedures.. Assessment of acute and subacute models of inflammation was using carrageenan-induced paw edema method and cotton pellet granuloma method using three dosage treatments; 200 mg/kg BW, 400 mg/kg BW, and 800 mg/kg BW along with a negative control group (0.5% Na CMC) and positive control (Na diclofenac 2.25 mg/kg BW). The inhibition period was observed at 30, 60, 90, 120, 150, and 180 min time intervals.Result: The phytochemical screening showed that the ethanolic fruit extract from Asam kandis contain contains flavonoids, glycosides, steroids, and triterpenoids. The anti-inflammatory result showed that the strongest inhibition produced by ethanolic fruit extract of Asam kandis occurred on the dosage of 800 mg/kg BW compared to the other doses (200 and 400 mg/kg BW) throughout the observation period.Conclusion: This finding indicated that ethanolic fruit extract of Asam kandis (G. xanthochymus Hook. f. ex T. Anderson) might become an interesting candidate for treatment of inflammation.

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