scholarly journals Effect of Indole-3-Butyric Acid on Clonal Propagation of Swietenia macrophylla through Branch Cutting

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Md. Salim Azad ◽  
Md. Abdul Matin
Keyword(s):  
Overall Survival ◽  
Butyric Acid ◽  
Clonal Propagation ◽  
Root Number ◽  
Swietenia Macrophylla ◽  
Rooted Cuttings ◽  
Survival Percentage ◽  
Shoot Number ◽  
Number Of Leaves ◽  
Branch Cutting

The study discloses the scopes of clonal propagation Swietenia macrophylla through branch cutting treated with IBA. A total of four hundred cuttings were used with four replications to assess the rooting ability. The study exposed significant (p<0.5) difference of rooted cuttings among the treatments. The highest (62.51%) rooting percent was observed in cutting with 0.4% IBA treatment. In addition, root number and its length per cutting were increased with increasing IBA concentration (p<0.5). The experiment showed significant (p<0.5) difference of sprouting among the treatments. The highest (67.27%) percent of sprouting was observed in cuttings with 0.4% solution, which showed a similar fashion of percent of rooting. The study also showed significant (p<0.5) difference of shoot number per cutting, the length of the longest shoot, and number of leaves per cutting among the treatments. The overall survival of rooted cuttings after transfer to polybag significantly (p<0.5) differed among the cuttings treated with different IBA solution. The highest survival percentage (69.67%) was observed in the cuttings rooted with 0.4% IBA treatment and the lowest (55.6%) survival was found in cuttings treated with control. The use of 0.4% IBA treatment is suggested for rooting of juvenile leafy branch cutting of S. macrophylla.

EFFECT OF GROWTH REGULATORS ON ROOTING OF CUTTINGS IN PLUM CV. KALA AMRITSARI

2018 ◽  
Vol 5 (25) ◽  
Author(s):  
Shagufi Narula
Keyword(s):  
Growth Regulators ◽  
Dry Weight ◽  
Shoot Length ◽  
Root Number ◽  
Fresh Weight ◽  
Rooted Cuttings ◽  
Survival Percentage ◽  
Significant Survival ◽  
Shoot Number ◽  
Rooting Of Cuttings

1200 cuttings made from the trees of Plum cv. Kala Amritsari were planted in 60 beds of size 1×1m., with 20 cuttings on each bed. Uniform sized 40 cuttings per replication were treated with ten treatments of IBA and PHB growth regulators by slow dip (24 hours) and quick dip (2 minutes) method before planting along with control. Results showed that IBA@2000ppm quick dip was found to be significant in improving the shoot and root characters in cuttings with maximum shoot length (16.87cm), average shoot girth (0.46cm), shoot number (4.22), fresh weight of shoots (6.82), dry weight of shoots (1.43g), leaf number (154.28), average leaf area (305.79 cm2), root number (44.90), average root length (10.88cm), length of longest root (13.5cm), fresh weight of roots (1.52g), dry weight of roots (1.09). While the cuttings treated with slow dip of IBA 150 ppm exhibited significant survival percentage (81.00%), percentage of rooted cuttings (74.33%) and sprouting percent (75.17%).

scholarly journals Three Mussaenda Cultivars Propagated by Stem Cuttings Exhibit Variation in Rooting in Response to Hormone and Rooting Conditions

2000 ◽  
Vol 10 (4) ◽  
pp. 780-784
Author(s):  
Rolston St. Hilaire ◽  
Carlos A. Fierro Berwart
Keyword(s):  
Butyric Acid ◽  
Root Length ◽  
Clonal Propagation ◽  
Stem Cuttings ◽  
Root Number ◽  
Root Numbers ◽  
Rooting Medium ◽  
Rooting Of Cuttings

The effects of 1H-indole-3-butyric acid (IBA), cutting position on stock plants, the date of propagation, the type of rooting substrate and temperature on rooting of mussaenda (Mussaenda erythrophylla Schumach. & Thonn. `Ashanti Blood' and `Rosea', and Mussaenda philippica A. Rich `Aurorae') stem cuttings were determined. Cuttings of `Ashanti Blood' produced the largest number of roots when treated with 15 mmol (3000 ppm) IBA and rooted in perlite at 29 °C (84 °F). Cutting position on stock plants did not affect rooting in any of the three cultivars. Propagation date and temperature of the rooting medium affected root numbers in `Aurorae'. With `Rosea', only the type of rooting substrate affected root number. Rooting percentage was 22%, 48%, and 39% in `Ashanti Blood', `Aurorae', and `Rosea' respectively. After 30 days of propagation average root length was 4, 12, and 4 mm (0.2, 0.5, and 0.2 inch) in `Ashanti Blood', `Aurorae', and `Rosea' respectively. Growers must determine precise rooting conditions for each cultivar to obtain consistent rooting of cuttings. This process may not be economically feasible on a commercial basis because rooting percentages are relatively low. We conclude that other methods of clonal propagation need to be evaluated before uniform rooted stem cuttings of mussaenda can be produced economically.

scholarly journals CLONAL PROPAGATION OF LIATRIS PYCNOSTACHYA MICHX BY IN VITRO CULTURE OF AXILLARY BUDS

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1137c-1137
Author(s):  
Azza Tawfik ◽  
P. E. Read ◽  
S. S. Salac
Keyword(s):  
In Vitro Culture ◽  
Bacterial Contamination ◽  
Clonal Propagation ◽  
Shoot Length ◽  
Axillary Buds ◽  
Shoot Number ◽  
Bud Growth ◽  
Number Of Leaves ◽  
Axillary Bud Growth

A method is described for obtaining explants free of bacterial contamination and for clonal propagation by in vitro culture of liatris axillary buds. Axillary bud growth was stimulated by removal of the shoot tips of greenhouse grown stock plants. Prior to using this approach, extreme bacterial contamination occured when explants were taken from stock plants that had not been decapitated. However, these axillary buds (0.3-0.5 cm long) were successfully established free of bacterial contamination when excised, surface disinfested and cultured on Murashige & Skoog (MS) medium supplemented with various levels of benzyladenine (BA) or kinetin and gibberellic acid (GA3). The highest number of leaves and greatest shoot length were produced by buds cultured on a medium supplemented with 1.0 mg/l BA plus 1.5 mg/l GA3. Shoot number was increased on medium containing 1.0 or 2.0 mg/l BA plus 0.5 mg/l GA3. Kinetin significantly increased the leaf number of the buds but there was no effect of kinetin on the shoot length or number. Shoots formed roots in a medium supplemented with 3 mg/l indole-3-butyric acid (IBA) plus 9 mg/l GA3. The plantlets were transferred to vermiculite and acclimatized successfully under intermittent mist in a greenhouse.

scholarly journals Respon Setek Cabang Bambu Betung (Dendrocalamus Asper) Akibat Pemberian Asam Indol Butirat (AIB)

2016 ◽  
Vol 4 (2) ◽  
pp. 61
Author(s):  
Erviana Sari ◽  
Indriyanto . ◽  
Afif Bintoro
Keyword(s):  
Butyric Acid ◽  
Experimental Unit ◽  
Development Research ◽  
Indole Butyric Acid ◽  
Randomized Design ◽  
Shoot Number ◽  
Bamboo Plantation ◽  
Dendrocalamus Asper ◽  
Completely Randomized Design ◽  
Branch Cutting

Betung bamboo (Dendrocalamus asper) as multipurpose plant could be developed with vegetative and generative propagation.  Branch cuttings was the easiest way in the betung bamboo vegetative propagation.  One of plant growth regulators that could be used to spur betung bamboo branch cutting was indole butyric acid (IBA).  This study was conducted to evaluate the betung bamboo branch cutting response from various concentration of IBA and to know the best concentration of IBA to spur the growth.  The experiment was conducted in Palembang’s Bamboo Plantation Collection and Development Research Institute of Forest areal, Tanjung Agung, District Tanjungan, South Lampung Regency, from March to June 2012.  This research used Completely Randomized Design (CRD) with five treatments: IBA 0 ppm (control), 100 ppm, 200 ppm, 300 ppm, and 400 ppm of concentration.  Each of experimental unit consisted of five cuttings with five replications.  Percentage of life, length of shoot, number of shoot, number of leave, shoot’s diameter, and root’s length were the observed variables.  The result showed that betung bamboo branch cuttings were responsive to IBA, and IBA 200 ppm concentration gave better results to the betung bamboo branch cuttings than IBA 0 ppm (control), 100 ppm, 300 ppm, and 400 ppm concentration. Based on this study, it was recomended to use 200 ppm of IBA for betung bamboo branch cutting. Keywords : betung bamboo, branch cutting, indole butyric acid

scholarly journals Effects of Phytohormones and Alternative Sources on the Propagation of Ternstroemia cameroonensis Cheek. by Marcotting in the Lebialem Highlands, Cameroon

2021 ◽  
pp. 52-60
Author(s):  
Francoline Jong Nkemnkeng ◽  
Mendi Grace Anjah ◽  
Walter Ndam Tacham ◽  
Christiana Ngyete Nyikob Mbogue ◽  
Victor-François Nguetsop
Keyword(s):  
Overall Survival ◽  
Forest Management ◽  
Butyric Acid ◽  
Environmental Conditions ◽  
Vegetative Propagation ◽  
Root Length ◽  
Mortality Rates ◽  
Routine Practice ◽  
Survival Percentage ◽  
Alternative Sources

Marcotting is a method of vegetative propagation which still finds its relevance in the present day forest management and routine practice to produce viable seedlings for the regeneration of Ternstroemia cameroonensis. There is dearth of information regarding the propagation of T. cameroonensis by marcotting. Hence effects of phytohormone application (Indole-3-acetc acid, Indole-3-butyric acid) and alternative sources (coconut water) on the propagation of T. cameroonensis by marcotting were investigated in the Lebialem Highlands. Mortality rates, number of roots, root length as well as marcotts position in the crown was evaluated. The propagation trials were carried under natural environmental conditions. Marcotts had an overall survival percentage of 35.41%. Those established at the middle of the crown had the highest survival percentage (15.27%) followed by those at the lower (11.80 %). Also marcotts established on branches with larger diameter (4-6 cm) and shorter length (50 cm) had the highest survival percentage (20.13 and 19.44 respectively). Again marcotts treated with IBA and CW had the best performance compared to other pre-treatments. According to the result, T. cameroonensis can be amenable through marcotts. 

scholarly journals Role of Auxin and Microclimate on the Success of Rooted Cuttings of Cocoa

2007 ◽  
Vol 23 (1) ◽  
Author(s):  
Adi Prawoto ◽  
Arifin . ◽  
Syamsul Bachri ◽  
K.C Setyaningtyas
Keyword(s):  
Light Intensity ◽  
Butyric Acid ◽  
Root Number ◽  
Indole Butyric Acid ◽  
Rooted Cuttings ◽  
Randomized Design ◽  
Auxin Content ◽  
Main Plot ◽  
Genetic And Environmental Factors ◽  
Available Technology

In Indonesia, cocoa reproduction by cuttings is undeveloped yet because the available technology is more expensive than the other clonal reproduction methods. The success of cocoa cuttings is influenced by genetic and environmental factors. The purpose of this research is to study effect of endogenous auxin content, effects of light intensity and exogenous auxin application, on the rooted cuttings. The 2 nd research purpose is to study effects of PVP (Polyvinylpyrrolidon) and IBA (β-indole-butyric acid), clones, and microclimate. The experiment was conducted in Kaliwining Experimental Station of Indonesian Coffee and Cocoa Research Institute in Jember (45 m a.s.l. and D rainfall type according to Schmidt Ferguson). The design for the 1 st experiment was splitsplit plot, replicated three times. The main plot was light intensity inside the roof, i.e.15%, 30%, and 45% to direct sun radiation. The sub plot was cocoa clones, i.e. DR 2 and ICS 13, and the sub-sub plot was IBA concentration, i.e. 0 ppm, 1500 ppm, 3000 ppm and 4500 ppm. The 2 nd experiment was designed factorial 3 x 3 of CRD, replicated 3 times. Clones of KW 163, KW 162 and KW 165, and IBA at 0, 3000, and 6000 ppm were the factors. In the same time, effect of IBA 6000 ppm, PVP 6000 ppm IBA+PVP 6000 ppm, and control were observed using KW 165 clone, and designed in complete randomized design (CRD), replicated 3 times. The result showed that auxin content of ICS 13 was higher than DR 2 (62.67 ppm vs 40.90 ppm) so that gave higher rooted cuttings and more root number. Exogenous application of IBA improved auxin content of the cutting materials and promoted root growth. The optimum IBA concentration for root number was 3500 ppm. Light intensity of 45% improved number of rooted cuttings three times compared to 15%, however compared to the 2 nd research, percentage of rooted cuttings was still very low. Cocoa cutting method to gain rooted cuttings 80—90% has been obtained. The method was using IBA 3000 ppm or 6000 ppm mixtured with or without PVP 6000 ppm, conducted during rainy season, the nursery using permanent shade trees of leucena sp., temperature of 24—27oC and relative humidity was 78—87%. PVP was supposed inhibit oxidation of IBA so that the effect of IBA was more optimum. The response of KW 162, KW 163 and KW 165 clones to root were similar. Rooted cuttings during dry season was low due to the less fresh of cutting materials and high temperature inside the bed roof. Key Words: Theobroma cacao, cuttings, light intensity, auxin, β-indole-butyric acid, Polyvinylpyrrolidon

scholarly journals Clonal Propagation of Flacourtia indica for Ensuring Quality Planting Materials and Sustainable Supply of Edible Fruits

2017 ◽  
Vol 5 (1) ◽  
pp. 42
Author(s):  
Md. Alamgir Kabir ◽  
Md. Hasan Mehedi Khan ◽  
Md. Masudur Rahman ◽  
A.T.M. Rabiul Alam ◽  
S.M. Hemayet Jahan ◽  
...  
Keyword(s):  
Clonal Propagation ◽  
Low Cost ◽  
Block Design ◽  
Coarse Sand ◽  
Research Field ◽  
Poor People ◽  
Stem Cuttings ◽  
Root Number ◽  
Survival Percentage ◽  
Wild Fruit

The present study was carried out at the Agriculture research field, Patuakhali Science And Technology University(PSTU), Patuakhali, from March, 2015 to April, 2016 to explore the domestication potential and to evaluate the rooting performance of Flacourtia indica (katabohori), a wild fruit species in Bangladesh, through clonal propagation by stem cutting under 3 different doses of rooting hormone IBA (Indole Buetaric Acid) and planted in the perforated plastic tray filled with coarse sand and gravel placed in the non-mist propagator. The experiment was laid out following a Randomized Complete Block Design (RCBD) with 4 treatments and 4 replications (blocks). The treatments were T0= control, T1 = 0.2% IBA, T2 = 0.4% IBA, T3 = 0.8% IBA. The rooting ability of cuttings was significantly influenced by the application of IBA. The results showed that the highest rooting percentage (100) and maximum root number (8) of Flacourtia indica stem cuttings were obtained from the cuttings treated with 0.4% IBA followed by 0.2% IBA where as the longest root length (8.998 cm) was recorded with 0.2% IBA followed by 0.4% IBA. Survival percentage of the cutlings (the rooted cuttings) after 3 months of transferring them into poly bags was also significantly enhanced by exogenous rooting hormone (IBA) application. The highest survival percentage (84.5 %) was for the cuttings treated with 0.4% IBA followed by 0.2% IBA (71%). The similar trend was also observed for average cutling height and number of leaf. Findings of the present study reveal that the plant species is highly amenable for clonal propagation by stem cuttings using low-cost non-mist propagator. Considering both rooting percentage and root number, 0.4% IBA treatment of stem cuttings may be recommended for mass production of quality planting stocks for the domestication of the species in homestead agroforestry or in fruit orchards to provide edible fruit to rural poor people of natural disaster vulnerable Bangladesh.

scholarly journals Cutting Type and IBA Treatment Duration Affect Teucrium fruticans Adventitious Root Quality

2014 ◽  
Vol 42 (2) ◽  
pp. 478-481 ◽  
Author(s):  
Leo SABATINO ◽  
Fabio D’ANNA ◽  
Giovanni IAPICHINO
Keyword(s):  
Acid Solution ◽  
Butyric Acid ◽  
Root Development ◽  
Root Length ◽  
Treatment Duration ◽  
Acid Treatment ◽  
Stem Cuttings ◽  
Root Number ◽  
Rooted Cuttings ◽  
Root Quality

Root development of stem cuttings of Silver Germander (Teucrium fruticans) was investigated in relation to cutting type and indole-3-butyric acid (IBA) treatment. Terminal cuttings of a clone grown in Sicily were trimmed to three types: terminal cuttings with apex (TWA), terminal cuttings without apex (TWOA) or sub-terminal cuttings (ST). To verify the cutting response to exogenous auxin, cuttings were dipped to a 2.0 cm depth in a 0.5% indole-3-butyric acid solution for 0, 5 or 7 minutes. Overall percent survival was 97 to 98%. Rooting percent, root number and root length were affected by cutting type and indole-3-butyric acid treatment. In general, TWA cuttings demonstrated a higher capacity to form roots than cuttings without apex (TWOA and ST cuttings). In absence of indole-3-butyric acid treatment, TWA and ST cuttings gave higher rooting percentages than TWOA cuttings. Exposing cuttings to indole-3-butyric acid improved percent rooting, number of roots per cutting and root length. The best results in terms of rooting percentage and root number per cuttings were obtained with TWA cuttings in combination with 7 min indole-3-butyric acid basal dip. However, cuttings taken farther down the stem, such as sub-terminal cuttings gave satisfactory rooting performance as well. We suggest that the use of all cutting types tested associated to indole-3-butyric acid basal dip for 5 or 7 min may be beneficial to propagators wishing to produce T. fruticans rooted cuttings with well-developed root system.

scholarly journals Influence of NAA and coconut water with variation of soaking duration on the growth of yellow bamboo branch cutting

2018 ◽  
Vol 10 (3) ◽  
pp. 178-182
Author(s):  
TIA SETIAWATI ◽  
AGINTA PUTRI REHULINA KELIAT ◽  
RULY BUDIONO ◽  
RUHYAT PARTASASMITA ◽  
JOHAN ISKANDAR
Keyword(s):  
Leaf Area ◽  
Root Length ◽  
Raw Materials ◽  
Shoot Length ◽  
Coconut Water ◽  
Root Number ◽  
Randomized Design ◽  
Shoot Number ◽  
Bambusa Vulgaris ◽  
Branch Cutting

Setiawati T, Keliat APR, Budiono R, Partasasmita R, Iskandar J. 2018. Influence of NAA and coconut water with variation of soaking duration on the growth of yellow bamboo branch cutting. Nusantara Bioscience 10: 178-182. Yellow bamboo (Bambusa vulgaris Schrad Ex. var. Striata) is one kind of bamboo used for industrial and household raw materials. Yellow bamboo propagation with branch cuttings has relatively low percentage of growth. To increase the growing success of yellow bamboo branch cuttings can be used growth regulators substance, such as NAA and coconut water. This study aimed to obtain the best combination of NAA and coconut water with the soaking duration in the growth of yellow bamboo branch cuttings. The study was conducted using Completely Randomized Design (CRD) of 6 × 3 factorial arrangement with five replications. The first factor was the combination of NAA and coconut water (CW), which consisted of six levels, namely 100% CW, 20% NAA + 80% CW, 40% NAA + 60% CW, 60% NAA + 40% CW, 80% NAA + 20% CW and 100% NAA. The second factor was the soaking duration which consists of three levels, namely 12 h, 24 h, and 36 h. Parameters observed included shoot emerging time, shoot number, shoot length, leaf area, root number and root length. The data obtained were analyzed using the Analysis of Variance (ANOVA) and Duncan's Multiple Range Test (DMRT) α=5%. The results showed that the combination of 80% NAA + 20% CW gave the best yellow bamboo branch cuttings growth with an average shoot length of 1.44 cm, leaf area 41.29 cm2, root number 42.87 and root length 23.70 cm. The interaction of 80% NAA + 20% CW with soaking duration of 36 h resulted in average the fastest shoot emerging time of 2.02 days after planting.

scholarly journals SOLUÇÕES DE NIACINA E ESTACAS COM DIFERENTES NÚMEROS DE GEMAS PARA PROPAGAÇÃO DE AMORA-PRETA

Nativa ◽  
2018 ◽  
Vol 6 (2) ◽  
pp. 134
Author(s):  
Eduardo Pradi Vendruscolo ◽  
Luiz Fernandes Cardoso Campos ◽  
Alexsander Seleguini
Keyword(s):  
Vitamin B6 ◽  
Root Elongation ◽  
Plant Hormone ◽  
Vegetative Reproduction ◽  
Axillary Buds ◽  
Root Number ◽  
Rooted Cuttings ◽  
Rubus Fruticosus ◽  
Number Of Leaves ◽  
Number Of Shoots

A propagação da amora-preta é comumente realizada de forma assexuada por estacas, assim a geração de informações para aprimoramento desta técnica é fundamental. O objetivo do trabalho foi avaliar a viabilidade da utilização de estacas lenhosas, com três ou quatro gemas axilares e tratamento com soluções de niacina para a reprodução vegetativa de amoreira-preta. O delineamento experimental utilizado foi inteiramente casualizado, com quatro repetições, perfazendo um fatorial 2 x 5, correspondente ao número de gemas axilares (três e quatro gemas) e a cinco concentrações de niacina (0, 100, 200, 300 e 400 mg L-1). Decorridos 100 dias, foi avaliada a taxa de sobrevivência, a porcentagem de estacas enraizadas, o número de brotações por estacas vivas, o número de folhas por brotação, o número de raízes por estaca viva e o comprimento da maior raiz. Estacas com quatro gemas axilares elevaram significativamente as taxas de sobrevivência e enraizamento, enquanto que estacas com três gemas favoreceram o alongamento das raízes. A utilização de soluções de niacina elevou o número de brotações em estacas com três gemas em 63% e o número de folhas por brotação, o número de raízes e o comprimento radicular em 79,1%, 156,8% e 46,9, respectivamente.Palavra-chave: Rubus fruticosus L., vitamina B6, reprodução assexuada, reservas nutricionais, fitormônios. NIACIN SOLUTIONS AND CUTTINGS WITH DIFFERENT NUMBERS OF BUDS FOR PROPAGATION OF BLACKBERRY ABSTRACT:The propagation of this species is usually carried out in an asexual manner by cuttings, so the generation of information to improve this technique is fundamental. The objective of the present study was to evaluate the feasibility of the use of woody cuts, with three or four axillary buds and treatment with niacin solutions for the vegetative reproduction of blackberry. The experimental design was completely randomized, with four replicates, with a factorial of 2 x 5, corresponding to the number of axillary buds (three and four buds) and five concentrations of niacin (0, 100, 200, 300 and 400 mg L-1). After 100 days, the survival rate, the percentage of rooted cuttings, the number of shoots per live cuttings, the number of leaves per shoot, the number of roots per live cutting and the length of the largest root were evaluated. Cuttings with four buds significantly increased survival and rooting rates, while cuttings with three buds favored root elongation. The use of solutions of niacin increased the number of shoots in cuttings with three axillary buds in 63% and the number of leaves per shoot, root number and root length in 79.1%, 156.8% and 46.9%, respectively.Keywords: Rubus fruticosus L., vitamin B6, assexual reproduction, nutricional reserves, plant hormone. DOI:

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