scholarly journals Antibacterial Efficacy of Nanoparticle-Incorporated Root Canal Sealer against Common Endodontic Pathogens - An in vitro Study

2020 ◽  
pp. 68-74
Author(s):  
Srujana Hemmanur ◽  
Iffat Nasim
Keyword(s):  
Silver Nanoparticles ◽  
Root Canal ◽  
In Vitro Study ◽  
Root Canal Treatment ◽  
Antibacterial Efficacy ◽  
Zone Of Inhibition ◽  
Product Analysis ◽  
Test Product ◽  
Zones Of Inhibition

Infection caused by microorganisms play a crucial role in the induction of inflammation of pulpal as well as the periapical tissues. Factors like improper disinfection or obturation of the root canal space contributes to the failure of the root canal treatment. The aim of the study was to modify the contents of MTA-based sealer with nanoparticles and check for its antibacterial efficacy against E. faecalis. Silver nanoparticles (10 nm) were incorporated in MTA based sealer at various concentrations to form the test product. The antibacterial efficacy of the modified sealer was tested by well diffusion test on E. faecalis. The zone of inhibition (mm) was checked for each test product. Analysis of the results showed significant diameters of zones of inhibition (mm) as compared to sealer without nanoparticles. The zone of inhibition increased with the increase in the concentration of silver nanoparticles. The silver nanoparticle incorporated sealer can be used in the clinical setup to prevent reinfection of the root canal system and ensure the success of the root canal treatment.

The in vitro evaluation of antibacterial efficacy optimized with cellular apoptosis on multi-functional polyurethane sealers for the root canal treatment

2021 ◽  
Vol 9 (5) ◽  
pp. 1370-1383
Author(s):  
Xiaoyu Lei ◽  
Jian Wang ◽  
Jie Chen ◽  
Jing Gao ◽  
Jinzheng Zhang ◽  
...  
Keyword(s):  
Root Canal ◽  
Evaluation System ◽  
Root Canal Treatment ◽  
Antibacterial Efficacy ◽  
Apoptotic Pathway ◽  
In Vitro Evaluation ◽  
Cellular Apoptosis ◽  
Genetic Assessment

Combined with a series of antibacterial tests and the genetic assessment of the apoptotic pathway, an evaluation system has been rationalized to govern the fate of the different compositions of PU-based sealers.

scholarly journals Antibacterial efficacy of biosynthesized silver nanoparticles against Enterococcus faecalis Biofilm: An in vitro study

2018 ◽  
Vol 9 (2) ◽  
pp. 237 ◽  
Author(s):  
KiranRahul Halkai ◽  
JayashreeA Mudda ◽  
Vasundhara Shivanna ◽  
Vandana Rathod ◽  
Rahul Halkai
Keyword(s):  
Silver Nanoparticles ◽  
Enterococcus Faecalis ◽  
In Vitro Study ◽  
Vitro Study ◽  
Antibacterial Efficacy

Comparative Evaluation of the Antimicrobial Effects of Different Mouthrinses against Streptococcus Mutans: An in Vitro Study

2019 ◽  
Vol 43 (6) ◽  
pp. 398-407
Author(s):  
Eman Abu-obaid ◽  
Fouad Salama ◽  
Ala’a Abu-obaid ◽  
Fars Alanazi ◽  
Mounir Salem ◽  
...  
Keyword(s):  
Reference Strain ◽  
In Vitro Study ◽  
Negative Control ◽  
Minimal Bactericidal Concentration ◽  
Distilled Water ◽  
Chlorhexidine Digluconate ◽  
Zone Of Inhibition ◽  
Antimicrobial Effects ◽  
Zones Of Inhibition

Purpose: To assess the antimicrobial effects of different natural and semi-natural mouthrinses on isolates of S. mutans obtained from the saliva of Saudi children and reference strains of S mutans (ATCC 25175). Study design: Saliva samples were collected from 20 children. Natural and semi-natural mouthrinses included were herbal mix mouthrinse, cranberry mouthrinse, chlorhexidine digluconate mouthrinse, cranberry extract mixed with chlorhexidine digluconate mouthrinse, chlorhexidine digluconate mouthrinse with alcohol (positive control), and distilled water (negative control). The microbiological examination tests were minimal inhibitory concentration, minimal bactericidal concentration, and zone of inhibition for the saliva isolates of S. mutans while zone of inhibition test only for reference strain of S. mutans. Results: For reference strain in a comparison with the distilled water, the herbal mix, cranberry, cranberry mixed with chlorhexidine, chlorhexidine, and chlorhexidine with alcohol showed significantly increased zones of inhibition by 36.38, 36.25, 26.13, 17.75, and 12.38, respectively. For saliva isolates in a comparison with the distilled water, the herbal mix, cranberry, cranberry mixed with chlorhexidine, chlorhexidine, and chlorhexidine with alcohol showed significantly increased zones of inhibition by 38.00, 34.25, 22.94, 16.50, and 16.44, respectively. Chlorhexidine with alcohol showed significantly lower minimum inhibitory and bactericidal concentration than the other groups. Conclusions: Herbal mix and cranberry mouthrinses could be effective natural alternative to chlorhexidine mouthrinse with or without alcohol in affecting tested parameters.

Effect of Calcium Hydroxide as a Root Canal Dressing Material on Dentin Fracture Strength In Primary Teeth–In Vitro Study

2018 ◽  
Vol 42 (2) ◽  
pp. 146-149
Author(s):  
Fadi Said ◽  
Moti Moskovitz
Keyword(s):  
Calcium Hydroxide ◽  
Fracture Strength ◽  
Root Canal ◽  
Primary Teeth ◽  
In Vitro Study ◽  
Vitro Study ◽  
Root Canal Treatment ◽  
Root Canals ◽  
Anterior Teeth

Objectives: The aim of the present study was to assess the effect of calcium hydroxide as a root canal dressing material on dentin microtensile fracture strength in human primary teeth in vitro. Study design: Thirty primary anterior teeth with root canals packed with calcium hydroxide were divided into groups of ten and immersed in saline at room temperature for 7, 30 and 90 days. Ten teeth with root canals filled with sterile saline were the control group. Microtensile fracture strength was measured in Mechanical tester Lloyd testing machine. Results: There was a significant difference (P < 0.05) between the fracture strength of the calcium hydroxide-filled teeth after 90 days (19.1 MPa) compared with the control (35.8 MPa). Dentin microtensile fracture strength of the calcium hydroxide-filled teeth decreased at an average of 0.142 MPa per day. Conclusion: Calcium hydroxide placed in root canals for an extended time had a significantly negative effect on root strength. Long-term success of root canal treatment in primary anterior teeth is estimated as 65% with most of the failures result from trauma recurrence. Clinical Relevance: Our results stress the need to evaluate the pros and cons of root canal treatment compared to extractions of non-vital primary incisors.

scholarly journals Antibacterial Efficacy of Neem, Triphala, Green Tea, and Combination of Neem with Triphala Extract against Enterococcus faecalis: An in vitro Study

2017 ◽  
Vol 6 (2) ◽  
pp. 65-67
Author(s):  
Neha Saini ◽  
Abhinav C Singhal ◽  
Aditi S Jain ◽  
Jayesh Tiwari ◽  
Pratik Surana
Keyword(s):  
Enterococcus Faecalis ◽  
Green Tea ◽  
Root Canal ◽  
Antibacterial Efficacy ◽  
Herbal Extracts ◽  
Zone Of Inhibition ◽  
Canal System ◽  
Root Canal System ◽  
Tea Extract

ABSTRACT Introduction The main objective of root canal treatment is to disinfect the entire root canal system, and irrigation is an important step in reducing the bacterial load from the root canal system. However, irrigants currently used in the field of endodontics have their share of limitations, and the search for an ideal root canal irrigant continues. The use of herbal extracts as endodontic irrigants is today gaining popularity. Aims and objectives To evaluate and compare the antimicrobial potential of herbal extracts, such as neem (Azadirachta indica), triphala (Terminalia chebula), green tea (Camellia sinensis), and combination of neem and triphala as endodontic irrigants against Enterococcus faecalis. Materials and methods Freshly prepared extracts of neem, triphala, green tea, and 2% chlorhexidine were used to assess the antimicrobial efficiency against E. faecalis using the agar well diffusion test. Agar plates were incubated at 370°C for 24 hours in an incubator. The diameter of bacterial inhibition zones around each well was recorded to the nearest size in mm. Results Higher mean zone of inhibition was recorded in chlorhexidine followed by neem extract and combination of neem and triphala than triphala and green tea extract respectively. The lowest mean zone of inhibition was found in green tea extract. Conclusion From the present study, it can be concluded that neem leaf extract shows comparable zones of inhibition with that of chlorhexidine and combination of neem and triphala. How to cite this article Singhal AC, Jain AS, Tiwari J, Surana P, Saini N. Antibacterial Efficacy of Neem, Triphala, Green Tea, and Combination of Neem with Triphala Extract against Enterococcus faecalis: An in vitro Study. Int J Experiment Dent Sci 2017;6(2):65-67.

Comparison of the Antibacterial Efficacy of Silver Nanoparticles with Chlorhexidine Against Enterococcus faecalis Biofilm—An In Vitro Study

2017 ◽  
Vol 17 (7) ◽  
pp. 4613-4617 ◽  
Author(s):  
Venkateshbabu Nagendrababu ◽  
Gowri Meiyazhagan ◽  
Winfred Sofi Beaula ◽  
Rajeswari Kalaiselvam ◽  
Balakumar Subramanian ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Enterococcus Faecalis ◽  
In Vitro Study ◽  
Vitro Study ◽  
Antibacterial Efficacy

Separated Instrument Management as a Procedural Accident in Endodontics Using the EDTA (C10H16N2O8) Aided Bypassing Technique and the Ultrasonic Removal Method. An in vitro Study

2020 ◽  
Vol 71 (11) ◽  
pp. 73-79
Author(s):  
Timea Dako ◽  
Cristina Ioana Bica ◽  
Luminita Lazar ◽  
Ana Petra Lazar ◽  
Monica Monea
Keyword(s):  
Root Canal ◽  
Ethylenediaminetetraacetic Acid ◽  
Small Diameter ◽  
In Vitro Study ◽  
Root Canal Treatment ◽  
Removal Method ◽  
Root Canals ◽  
Poor Access ◽  
Bypass Technique

Fracture of endodontic files can be considered a real concern during root canal treatment. This procedural accident is caused both by overusing instruments due to economic reasons and using an incorrect technique in root canals with difficult anatomy. A broken endodontic file does not always regard the treatment as a failure. Frequently the broken fragment can be bypassed, and the root canal treatment completed. In other cases, when the file is separated in a tooth with associated apical disease, its retrieval using ultrasonic tips is advised. Separation of a file usually occurs in molars, mainly in the mesiobuccal canal due to major curvature, poor access or small diameter. Our research was conducted to determine the in vitro efficiency of the ultrasonic removal method and the bypass technique by using 40 extracted molars with moderately curved roots. #25 K-files were deliberately broken in the middle third of mesiobuccal root canals after making a small excavation in the apical third of their active part. The results showed a significantly higher success rate of the ethylenediaminetetraacetic acid (EDTA) aided bypassing technique compared to the ultrasonic removal method.

scholarly journals COMPARISON OF ANTIFUNGAL EFFECTS OF COMMERCIALLY AVAILABLE HERBAL MOUTHWASHES AND CHLORHEXIDINE AGAINST CANDIDA ALBICANS IN DIABETIC PATIENTS: AN IN VITRO STUDY

2016 ◽  
Vol 9 (5) ◽  
pp. 214
Author(s):  
Chandini Ravikumar ◽  
Radhika Arjunkumar ◽  
Gopinath Prakasam
Keyword(s):  
Diabetes Mellitus ◽  
Candida Albicans ◽  
Periodontal Diseases ◽  
In Vitro Study ◽  
Average Diameter ◽  
Diabetic Patients ◽  
Zone Of Inhibition ◽  
Zones Of Inhibition ◽  
Vitro Effect

ABSTRACTObjective: Candida albicans is a part of the normal flora of the mouth in diabetes mellitus (DM) patients. Periodontitis is one of the main complicationsin diabetic patients. Mechanical and chemical plaque control are the most productive methods in preventing periodontal diseases in the oral cavity.The objective of this study is to compare the in vitro effect of herbal mouthwashes and chlorhexidine (CHX) against C. albicans.Methods: Saliva samples were obtained from diabetic patients reporting for treatment to Saveetha medical college. C. albicans was cultured from thesalivary sample. A yeast suspension was made by sub culturing the C. albicans. The mouthwashes used in the study are HiOra regular (0.2%), HiOrasensitive (0.5%), and clohex plus (CHX gluconate 0.02%) mouthwashes. The fungal suspension was spread on Sabouraud’s dextrose agar (SDA) plateswith a sterile swab. Subsequently, wells of 6 mm in diameter were made with a suitable distance using sterile cork borer on pre-inoculated agar platesand filled with 100 µl of each mouthwashes. From the zones of inhibition seen, antimicrobial activity was expressed in terms of average diameter ofthe zones of inhibition measured.Results: Using HiOra regular mouthwash, 13/18 (72%) wells were found to show zone of inhibition ≥20 mm. In HiOra sensitive mouthwash, only9/18 (50%) showed inhibition zone ≥20 mm. With effect of regular CHX mouthwash, none of the strains showed the zone of inhibition to be ≥20 mm.Most of the strains responded well with all the three mouthwashes.Conclusion: Among the 2 herbal mouthwashes, HiOra regular mouthwash was most effective in inhibiting the candidal growth when compared tothe HiOra sensitive. HiOra regular mouthwash still showed better inhibitory actions when compared to the regular CHX mouthwash and the candidalspecies showed increased sensitivity to it.Keywords: Candida albicans, Chlorhexidine mouthwash, Diabetes mellitus, Herbal mouthwashes, Zone of inhibition.

scholarly journals Antibacterial Efficacy of a Dispersion of Silver Nanoparticles in Citrate Medium for the Treatment of E. faecalis: an In Vitro Study

2016 ◽  
Vol 18 (2) ◽  
pp. 99
Author(s):  
Silvia Rodríguez DDS ◽  
Tatiana Ramírez DDS, MSc ◽  
Mauricio Montero DDS, MSc ◽  
Daniel Chaparía DDS, MSc, PhD ◽  
Grettel Valle PhD ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Saline Solution ◽  
Wild Strain ◽  
In Vitro Study ◽  
Bacterial Count ◽  
Negative Control ◽  
Antibacterial Efficacy ◽  
Sterile Saline ◽  
Negative Controls

The purpose of this study was to measure the antibacterial efficacy of a dispersion of silver nanoparticles (AgNP) in a citrate medium tested in two E. faecalis strains. AgNP were synthesized, and AgNP citrate medium (AgNP-CM) dispersion was prepared at a concentration of 100 μg/mL. The antibacterial efficacy of AgNP-CM dispersion was evaluated over two E. faecalis strains: ATCC29212 and a wild strain collected from human necrotic teeth. 5% sodium hypochlorite (NaOCl) and sterile saline solution were used as positive and negative controls. 5 and 30-minute contact tests were conducted and each experimental group were replicated 10 times. After 24 hours of incubation, the Log CFU/mL were calculated. The AgNP obtained showed spherical shapes and had 30-60nm size. 5% NaOCl was able to completely eliminate both E. faecalis strains in all groups, showing a significant statistical difference when compared to AgNP-CM dispersion and negative control groups. AgNP-CM dispersion showed a statistically significant decrease in Log CFU/mL averages (p=0,0006) when compared to the sterile saline solution for the ATCC29212 strain during the 30-minute time. Between the 5-minute and 30-minute groups, a significant bacterial count decrease was also observed (p= 0,0128). The antibacterial efficacy of the dispersion was greater for the ATCC29212 strain than the wild strain, were the effect diminished. AgNP-CM dispersion showed a significantly lower antibacterial efficacy against E. faecalis than the 5% NaOCl at the tested times. 

Sign in / Sign up

Export Citation Format

Share Document