scholarly journals Assessment of Bacterial Micro Colonies Present in Tissue Samples Obtained Using Ozonated Water as an Irrigating Agent from Patients with Periodontitis

2020 ◽  
pp. 90-97
Author(s):  
Niveda Rajeshwaran ◽  
Sankari Malaippan
Keyword(s):  
Surgical Procedure ◽  
Saline Water ◽  
Periodontal Diseases ◽  
Gingival Tissue ◽  
Distilled Water ◽  
Scaling And Root Planing ◽  
Tissue Samples ◽  
Root Planing ◽  
Colony Forming Units ◽  
Ozonated Water

Periodontal diseases are chronic inflammatory diseases that are constantly associated with microorganism, conventionally scaling and root planing followed by flap surgical procedure is done to treat chronic periodontitis condition, irrigating agents are used during both sub gingival scaling and root planing procedure and in flap surgical procedure. Saline and distilled water are the commonly used irrigants and they non specifically reduce the bacterial count, Ozonised water is also used as an irrigating agent. Though it’s effect on plaque is known to be effective its effect on tissues is still questionable. The study is conducted to assess the number bacterial micro colonies formed in gingival tissues after irrigating with ozonated water and comparing it with distilled water and saline water which are the commonly used irrigating agents. The aim of the study is to assess the bacterial micro colonies formed in gingival tissues after irrigating with ozonated water and comparing it with distilled water and saline water which are the commonly used irrigating agents. A randomized, split mouth study was performed. A total of 10 patients suffering from chronic generalised periodontitis were recruited into the study; 20 gingival tissue samples were obtained from the patients at the time of surgery. Both the patient and the clinician were blinded regarding the type of the irrigating agent used. The plaque samples were transferred into carrier medium and were cultured for 24 hours; 24 h after culture, the samples were collected and total colony forming units were calculated. On  counting  the number of  colony forming  units formed, it  was  found   that  the  amount  of colonies formed  from the samples treated with  ozone  showed mixed results, wherein it showed both increase and decrease in colony counts.

scholarly journals COMPARATIVE ESTIMATION OF HEAT SHOCK PROTEIN 70 (HSP 70) EXPRESSION IN HEALTH, GINGIVITIS AND PERIODONTITIS.

2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Dhivya Darshani D ◽  
Nithya anand ◽  
Dr. Vidyarani Shyamsundar ◽  
Dr.Bagavad Gita
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Heat Shock Protein 70 ◽  
Periodontal Diseases ◽  
Maxillofacial Surgery ◽  
Basal Layer ◽  
Gingival Tissue ◽  
Oral And Maxillofacial Surgery ◽  
Hsp 70 ◽  
Tissue Samples

Background: Although periodontal bacteria are the causative agents of periodontitis, subsequent progression and disease severity are thought to be determined by a host's immune responses. HSPs may be expressed during periodontal diseases. Objective: To compare the HSP 70 expression within layers of epithelium and connective tissue and correlate the expression of HSP 70 with the severity of periodontal disease. Materials & Methods: The study population comprised of 25 patients with periodontitis, and 23 patients with gingivitis and 21 controls with healthy gingival; tissue samples collected from the Department of Periodontology /Oral and Maxillofacial Surgery respectively. An immunohistochemical analysis was done for HSP 70 expression. Results: One way ANOVA results indicate a statistically significant expression in basal layer for mild inflammation (mean=2.36) and intensity in stratum spinosum for mild (mean=2.27) and minimum (mean=2.39) inflammation. An overall greater HSP 70 expression is noticed in periodontitis group (total IRIDI = 25.41) however not statistically significant Conclusion: We elucidated that there was significant HSP 70 expression in stratum basale and spinosum for mild inflammation that did not increase with severity of inflammation. The overall expression of HSP70 is insignificantly elevated in periodontitis compared to health & gingivitis. Statistical analysis used: One way Analysis of Variance (ANOVA), Mann Whitney U test and Kruskal Wallis Test. Keywords:  Heat shock protein 70 (HSP 70), Immunohistochemistry (IHC), Porphyromonas gingivalis.

scholarly journals Study of the Antimicrobial Effect of an Ethanolic Extract of Propolis in Periodontal Disease

2021 ◽  
Vol 11 (16) ◽  
pp. 7463
Author(s):  
Maria Jesús Lisbona-González ◽  
Esther Muñoz-Soto ◽  
Candela Reyes-Botella ◽  
Maria Victoria Olmedo-Gaya ◽  
Javier Diaz-Castro ◽  
...  
Keyword(s):  
Periodontal Disease ◽  
Therapeutic Potential ◽  
Periodontal Diseases ◽  
Test Group ◽  
Periodontal Therapy ◽  
Control Group ◽  
Clinical Parameters ◽  
Scaling And Root Planing ◽  
Root Planing ◽  
Supportive Periodontal Therapy

Periodontal disease encompasses gingivitis and periodontitis and is one of the most common chronic infections in the adult population. This study aimed to evaluate the influence of Spanish propolis extract (EEP) on the effect of the clinical and microbiological parameters as an adjuvant to scaling and root planning in patients undergoing supportive periodontal therapy (SPT). Forty chronic periodontitis patients were randomly assigned into two groups for the treatment. In the control group (n = 20), the sites were treated by scaling and root planing followed by gingival irrigation with physiological saline and in the test group (n = 20), the sites were treated by scaling and root planing followed by subgingival placement of EEP. At baseline (BL), bleeding on probing positive (BOP+) sites with probing pocket (PPD) ≥ 4 mm were defined as study sites. Plaque index, PPD, BOP, clinical attachment level (CAL), and subgingival plaque were evaluated at BL and 1 month later. The results showed a significant clinical improvement (p < 0.05) in the PPD, CAL and BOP+ comparing them with BL and one month after the periodontal treatment and a significant reduction (p < 0.05) for Tannerella forsythensis, Porphyromonas gingivalis, Prevotella intermedia and Treponema denticola in both groups. In addition, the improvement of clinical parameters was observed with subgingival use of EEP and also statistically significant differences between groups were observed (p < 0.05) such as reductions of BOP+ % and reduced counts of T. forsythensis and P. gingivalis, considered as the “key pathogens” for the periodontal diseases. Our results suggest prophylactic and therapeutic potential for EEP against periodontal diseases, improving clinical parameters, reducing gingival bleeding and decreasing bacterial counts of T. forsythensis and P. gingivalis. The subgingival use of EEP represents a promising modality as an adjuvant in periodontal therapy to avoid microbial resistance and other adverse effects.

scholarly journals RADIOGRAPHIC AND IMMUNO HISTOCHEMICAL DIAGNOSTIC STUDY OF STRONTIUM RANELATE AND METAL-SUBSTITUTED HYDROXYAPATITE BONE GRAFT MATERIALS IN DIABETES MELLITUS WITH CHRONIC PERIODONTITIS

2020 ◽  
Vol 8 (11) ◽  
pp. 473-483
Author(s):  
Khashaba Oh ◽  
◽  
Alasfar Aa ◽  
Elgendy Ea ◽  
El-Nagdy Sy ◽  
...  
Keyword(s):  
Strontium Ranelate ◽  
Chronic Periodontitis ◽  
Alveolar Bone ◽  
Periodontal Diseases ◽  
Diabetic Rats ◽  
Treatment Modalities ◽  
Gingival Tissue ◽  
Diagnostic Study ◽  
Tissue Samples ◽  
Periapical Radiography

Objective: The aim of the present study is to assess the radiographic and immunohistochemical effect of strontium ranelate (SR) and metal substituted hydroxyapatite (MSHA) on the treatment of chronic periodontitis among diabetic rats Materials and Methods: The study involved ten adult male and female rice rats (1-month-old) weighting (250- 300g). After a 24-hour fast, a single intraperitoneal dose of freshly prepared alloxan was injected to induce diabetes. A month after the injection of alloxan, the rats were randomly assigned to one of the two treatment modalities: SR with gengigel or MSHA with gengigel. Digital periapical radiography was taken at baseline, a month after the injection of alloxan to see resorbed alveolar bone and after 3months post-operative surgery for radiographic assessment. The diabetic rats were sacrificed using an overdose of anesthesia, and gingival tissue samples were collected. The specimens were processed for hematoxylin and eosin (H & E) staining and immune stain for expression of matrix metalloproteinases 2 (MMP-2). Results: Digital periapical radiography showed an increase of nearly 0.37 mm in the height of the alveolar bone in the side of the SR group while the other side in the MSHA group increased by nearly 0.12 mm. A statistically significant reduction in the expression of MMP2 in the SR group as compared to the MSHA group was found upon comparing the immunohistochemical results of the 2 groups. Conclusion: Radiographic and immunohistochemical results showed that SR was a promising material in the treatment of periodontal diseases.

scholarly journals Expression Profile of IL-35 mRNA in Gingiva of Chronic Periodontitis and Aggressive Periodontitis Patients: A Semiquantitative RT-PCR Study

2013 ◽  
Vol 35 ◽  
pp. 819-823 ◽  
Author(s):  
Nagaraj B. Kalburgi ◽  
Akshay Muley ◽  
B. M. Shivaprasad ◽  
Arati C. Koregol
Keyword(s):  
Mrna Expression ◽  
Chronic Periodontitis ◽  
T Regulatory Cells ◽  
Periodontal Diseases ◽  
Aggressive Periodontitis ◽  
Gingival Tissue ◽  
Regulatory Cells ◽  
Rt Pcr ◽  
Tissue Samples ◽  
Polymerase Chain

Background. Proinflammatory and anti-inflammatory cytokines play a key role in the pathogenesis of periodontal diseases. Secretion of bioactive IL-35 has been described by T regulatory cells () and is required for their maximal suppressive activity. are involved in the modulation of local immune response in chronic periodontitis patients.Objective. Hence, the present study was aimed to investigate the expression of IL-35 mRNA in chronic periodontitis and aggressive periodontitis patients.Materials and Methods. The present study was carried out in 60 subjects, which included 20 chronic periodontitis patients, 20 aggressive periodontitis patients, and 20 periodontally healthy controls. IL-35 mRNA expression in gingival tissue samples of all subjects was semiquantitatively analyzed using Reverse Transcriptase Polymerase Chain Reaction (RT-PCR).Results. The present study demonstrated the expression of IL-35 mRNA in gingival tissues of all the three groups. IL-35 mRNA expression was highest in chronic periodontitis subjects () as compared to the aggressive periodontitis group () and least seen in healthy patients ().Conclusion. The increased expression of IL-35 in chronic and aggressive periodontitis suggests its possible role in pathogenesis of periodontitis. Future studies done on large samples with intervention will strengthen our result.

scholarly journals Study on C-reactive Protein in Periodontal Diseases: Estimation and Correlation in Health, Gingivitis and Periodontitis and its Assessment after Scaling and Root Planing

2014 ◽  
Vol 3 (1) ◽  
pp. 4-7
Keyword(s):  
Periodontal Disease ◽  
Periodontal Diseases ◽  
Clinical Parameters ◽  
Scaling And Root Planing ◽  
C Reactive Protein ◽  
Inflammatory Biomarker ◽  
Reactive Protein ◽  
Root Planing ◽  
Health Group ◽  
Serum Crp

ABSTRACT Background Periodontitis is associated with elevated levels of systemic inflammatory biomarker C-reactive protein (CRP). This study is outlined to determine and correlate the C-reactive CRP level in health, gingivitis and periodontitis and to assess the CRP values after complete scaling and root planing. Materials and methods A total of 60 systemically healthy subjects enrolled in the study. Out of which 20 subjects each categorized under three groups, i.e. periodontally healthy, gingivitis and periodontitis. Clinical parameters and serum CRP is recorded at the baseline 15th and 30th day. Results Patients with chronic periodontitis had statistically significant elevations in serum CRP levels as compared to gingivitis and absence of detection of serum CRP level in health group. Results showed a highly significant change in clinical parameters and serum CRP with regard to improvement in the gingival inflammation and reduced bleeding on probing and plaque accumulation from the baseline when compared to the 15th day and 30th day. Conclusion A correlation exists between the periodontal disease and CRP, as the periodontal disease regresses the value of CRP lowers down significantly. Nonsurgical periodontal treatment, i.e. scaling and root planing was effective in reducing the levels of serum CRP. How to cite this article Saini R. Study on C-reactive Protein in Periodontal Diseases: Estimation and Correlation in Health, Gingivitis and Periodontitis and its Assessment after Scaling and Root Planing. Int J Experiment Dent Sci 2014;3(1):4-7.

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