scholarly journals Collagen-Chitosan Composite Powder as a Novel Biomaterial for Chronic Wounds in Hansen Disease-A Clinical Study

2021 ◽  
pp. 154-165
Author(s):  
Pombala Suresh Babu ◽  
Harini Srinivasan ◽  
B. Sai Dhandapani ◽  
C. Rose
Keyword(s):  
Wound Repair ◽  
Composite Powder ◽  
Chronic Wounds ◽  
Waste Product ◽  
Wound Dressings ◽  
Meat Industry ◽  
Before And After ◽  
Hematoxylin And Eosin ◽  
Chitosan Composite

Aims: Natural biomaterials are more suitable than synthetic biomaterials for in vivo applications for treating damaged tissues. Collagen and chitosan are abundantly available natural biomaterials for wound dressings for tissue/wound repair. In this context, collagen-chitosan composite powder has been used to treat chronic wounds in Hansen disease (HD) patients. Place and Duration of Study: CSIR Central Leather Research Institute, Sardar Patel Road, Adyar, Chennai 600021, Southern Railway Headquarters Hospital, Constable Road, Ayanavaram, Chennai 600023, and Gremaltes Hospital, India between June 2013 and July 2020. Methodology: Collagen extracted from bovine rumen, a waste product of meat industry, and a commercially available chitosan were prepared as a composite powder (COL/CS) and applied to chronic wounds in HD patients after debridement and the wound contours were measured by planimetry. Biochemical parameters in blood samples were periodically assessed. Histopathology of wound tissue with Hematoxylin and Eosin and Masson’s Trichrome staining was studied. Matrix Metalloproteinase-9 (MMP9) levels before and after treatment were estimated. Results: Wound healing of 64.2% was obtained with COL/CS treatment and formation of granulation cells was observed early. Hemogram studies have been reported in a regression model with 95% confidence intervals. Histopathology revealed dense collagen fibres and continuity of sub-epithelial layer on 8th day. MMP-9 levels showed collagen integrity after treatment. Conclusion: The novel biocompatible, biodegradable COL/CS wound dressing is a promising biomaterial for management of chronic wounds in Hansen disease patients.

scholarly journals Platelet-rich plasma accelerates skin wound healing by promoting re-epithelialization

2020 ◽  
Vol 8 ◽  
Author(s):  
Pengcheng Xu ◽  
Yaguang Wu ◽  
Lina Zhou ◽  
Zengjun Yang ◽  
Xiaorong Zhang ◽  
...  
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Wound Repair ◽  
Chronic Wounds ◽  
Platelet Rich Plasma ◽  
Skin Wound ◽  
Local Inflammation ◽  
Skin Wound Healing

Abstract Background Autologous platelet-rich plasma (PRP) has been suggested to be effective for wound healing. However, evidence for its use in patients with acute and chronic wounds remains insufficient. The aims of this study were to comprehensively examine the effectiveness, synergy and possible mechanism of PRP-mediated improvement of acute skin wound repair. Methods Full-thickness wounds were made on the back of C57/BL6 mice. PRP or saline solution as a control was administered to the wound area. Wound healing rate, local inflammation, angiogenesis, re-epithelialization and collagen deposition were measured at days 3, 5, 7 and 14 after skin injury. The biological character of epidermal stem cells (ESCs), which reflect the potential for re-epithelialization, was further evaluated in vitro and in vivo. Results PRP strongly improved skin wound healing, which was associated with regulation of local inflammation, enhancement of angiogenesis and re-epithelialization. PRP treatment significantly reduced the production of inflammatory cytokines interleukin-17A and interleukin-1β. An increase in the local vessel intensity and enhancement of re-epithelialization were also observed in animals with PRP administration and were associated with enhanced secretion of growth factors such as vascular endothelial growth factor and insulin-like growth factor-1. Moreover, PRP treatment ameliorated the survival and activated the migration and proliferation of primary cultured ESCs, and these effects were accompanied by the differentiation of ESCs into adult cells following the changes of CD49f and keratin 10 and keratin 14. Conclusion PRP improved skin wound healing by modulating inflammation and increasing angiogenesis and re-epithelialization. However, the underlying regulatory mechanism needs to be investigated in the future. Our data provide a preliminary theoretical foundation for the clinical administration of PRP in wound healing and skin regeneration.

scholarly journals Chitosan & Conductive PANI/Chitosan Composite Nanofibers - Evaluation of Antibacterial Properties

2019 ◽  
Vol 4 (1) ◽  
pp. 6-20 ◽  
Author(s):  
Panagiota Moutsatsou ◽  
Karen Coopman ◽  
Stella Georgiadou
Keyword(s):  
Chronic Wounds ◽  
Antibacterial Properties ◽  
Wound Dressings ◽  
Bacterial Strains ◽  
Healthcare Applications ◽  
E Coli ◽  
Jet Stability ◽  
Electrospun Membranes ◽  
New Treatments ◽  
Chitosan Composite

Background: Within the healthcare industry, including the care of chronic wounds, the challenge of antimicrobial resistance continues to grow. As such, there is a need to develop new treatments that can reduce the bioburden in wounds. Objective: The present study is focused on the development of polyaniline (PANI) / chitosan (CH) nanofibrous electrospun membranes and evaluates their antibacterial properties. Methods: To this end, experimental design was used to determine the electrospinning windows of both pure chitosan and PANI/CH blends of different ratios (1:3, 3:5, 1:1). The effect of key environmental and process parameters (relative humidity and applied voltage) was determined, as well as the effect of the PANI/CH ratio in the blend and the molecular interactions between PANI and chitosan that led to jet stability. Results: The nanofibrous mats were evaluated regarding their morphology and antibacterial effect against model gram positive and gram negative bacterial strains, namely B. subtilis and E. coli. High PANI content mats show increased bactericidal activity against both bacterial strains. Conclusion: The blend fibre membranes combine the materials’ respective properties, namely electrical conductivity, biocompatibility and antibacterial activity. This study suggests that electrospun PANI/CH membranes are promising candidates for healthcare applications, such as wound dressings.

scholarly journals Wound Healing Activities and Potential of Selected African Medicinal Plants and Their Synthesized Biogenic Nanoparticles

Plants ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 2635
Author(s):  
Caroline Tyavambiza ◽  
Phumuzile Dube ◽  
Mediline Goboza ◽  
Samantha Meyer ◽  
Abram Madimabe Madiehe ◽  
...  
Keyword(s):  
Wound Healing ◽  
Medicinal Plants ◽  
Wound Repair ◽  
Chronic Wounds ◽  
In Vivo Models ◽  
Repair Mechanisms ◽  
Treatment Of Wounds ◽  
Biogenic Nanoparticles

In Africa, medicinal plants have been traditionally used as a source of medicine for centuries. To date, African medicinal plants continue to play a significant role in the treatment of wounds. Chronic wounds are associated with severe healthcare and socio-economic burdens despite the use of conventional therapies. Emergence of novel wound healing strategies using medicinal plants in conjunction with nanotechnology has the potential to develop efficacious wound healing therapeutics with enhanced wound repair mechanisms. This review identified African medicinal plants and biogenic nanoparticles used to promote wound healing through various mechanisms including improved wound contraction and epithelialization as well as antibacterial, antioxidant and anti-inflammatory activities. To achieve this, electronic databases such as PubMed, Scifinder® and Google Scholar were used to search for medicinal plants used by the African populace that were scientifically evaluated for their wound healing activities in both in vitro and in vivo models from 2004 to 2021. Additionally, data on the wound healing mechanisms of biogenic nanoparticles synthesized using African medicinal plants is included herein. The continued scientific evaluation of wound healing African medicinal plants and the development of novel nanomaterials using these plants is imperative in a bid to alleviate the detrimental effects of chronic wounds.

scholarly journals Healing Effect of Vicenin-2 (VCN-2) on Human Dermal Fibroblast (HDF) and Development VCN-2 Hydrocolloid Film Based on Alginate as Potential Wound Dressing

2020 ◽  
Vol 2020 ◽  
pp. 1-15
Author(s):  
Woan Sean Tan ◽  
Palanisamy Arulselvan ◽  
Shiow-Fern Ng ◽  
Che Norma Mat Taib ◽  
Murni Nazira Sarian ◽  
...  
Keyword(s):  
Wound Healing ◽  
Drug Release ◽  
Wound Dressing ◽  
Wound Repair ◽  
Chronic Wounds ◽  
Dermal Fibroblast ◽  
Wound Dressings ◽  
Dependent Manner ◽  
Synthetic Compound ◽  
Study Results

Chronic wounds represent serious globally health care and economic issues especially for patients with hyperglycemic condition. Wound dressings have a predominant function in wound treatment; however, the dressings for the long-lasting and non-healing wounds are still a significant challenge in the wound care management market. Astonishingly, advanced wound dressing which is embedded with a synthetic drug compound in a natural polymer compound that acts as drug release carrier has brought about promising treatment effect toward injured wound. In the current study, results have shown that Vicenin-2 (VCN-2) compound in low concentration significantly enhanced cell proliferation and migration of HDF. It also regulated the production of pro-inflammatory cytokines such as IL-6, IL-1β, and TNF-α from HDF in wound repair. Treatment of VCN-2 also has facilitated the expression of TGF-1β and VEGF wound healing maker in a dose-dependent manner. A hydrocolloid film based on sodium alginate (SA) incorporated with VCN-2 synthetic compound which targets to promote wound healing particularly in diabetic condition was successfully developed and optimized for its physico-chemical properties. It was discovered that all the fabricated film formulations prepared were smooth, translucent, and good with flexibility. The thickness and weight of the formulations were also found to be uniform. The hydrophilic polymer comprised of VCN-2 were shown to possess desirable wound dressing properties and superior mechanical characteristics. The drug release profiles have revealed hydrocolloid film, which is able to control and sustain the VCN-2 released to wound area. In short, hydrocolloid films consisting of VCN-2 formulations are suitably used as a potential wound dressing to promote restoration of wound injury.

scholarly journals Selective antimicrobial activity of maggots against pathogenic bacteria

2008 ◽  
Vol 57 (5) ◽  
pp. 617-625 ◽  
Author(s):  
Domen Jaklič ◽  
Aleš Lapanje ◽  
Klemen Zupančič ◽  
Dragica Smrke ◽  
Nina Gunde-Cimerman
Keyword(s):  
Pathogenic Bacteria ◽  
Chronic Wounds ◽  
Antibacterial Activities ◽  
Bacterial Strains ◽  
Infected Wounds ◽  
Before And After ◽  
Treatment Of Wounds ◽  
Maggot Therapy

Maggot therapy, also known as biosurgery, is an ancient method for the healing of chronic infected wounds. Although clinicians have reported on the beneficial activities of the Lucilia sericata larvae that have been used for healing chronic wounds, the selectivity of this therapy against the different pathogenic micro-organisms that are found in chronic wounds has never been analysed. In the present study, we have investigated the in vitro activities of larval excreta/secreta both against selected bacterial strains that frequently occur in chronically infected wounds, and against bacteria isolated directly from the larvae and their excreta/secreta. Additionally, the antibacterial activities were investigated in in vivo studies, by comparing bacterial diversity in wounds before and after the application of L. sericata larvae. In conclusion, larval therapy is highly recommended, particularly for the treatment of wounds infected with Gram-positive bacteria, like Staphylococcus aureus, but less so for wounds infected with Gram-negative bacteria, especially Proteus spp. and Pseudomonas spp. strains. Bacteria from the genus Vagococcus were resistant to the maggot excreta/secreta.

657 Preliminary Study on the Effect of Various Antimicrobial Formulations Containing Silver Oxynitrate on Reducing Pseudomonas Aeruginosa Using an in-vivo Porcine Burn Wound Model

2020 ◽  
Vol 41 (Supplement_1) ◽  
pp. S174-S174
Author(s):  
Stephen C Davis ◽  
Joel Gil ◽  
Michael Solis ◽  
Alex Higa
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Untreated Control ◽  
Chronic Wounds ◽  
Wound Care ◽  
Wound Dressings ◽  
Bacterial Reduction ◽  
Burn Wound ◽  
Burn Wounds ◽  
Preliminary Study

Abstract Introduction Silver has long been known for its antimicrobial effects and has been commonly applied topically to burn wounds for years. More recently, wound dressings compounded with silver ions, have been developed to prevent and treat wound infection in both burn and chronic wounds. Methods This preliminary study evaluates the effect of a proprietary silver oxynitrate creams on Pseudomonas aeruginosa (PA) biofilms using a well-established burn wound porcine model model. Swine were used due to their skins similarities to humans and response to wound treatments. Briefly, second degree burn wounds were created and inoculated with PA. Wounds were then covered for 24 hours with a polyurethane dressing to allow for biofilm formation. The polyurethane dressing was removed and wounds were randomly assigned to one of the following treatment groups: 1) silver oxynitrate cream 0%, 2) silver oxynitrate cream 4%, 3) silver oxynitrate cream 7%, 4) silver oxynitrate cream * 10%, 5) silver oxynitrate powder, 6) silver sulfadiazine cream (SSD)~, or 7) untreated control. All treatments groups were covered with a polyurethane dressing to prevent any cross contamination. On days 3 and 7 after wounding wounds were cultured using an established scrub technique. Results Silver oxynitrate powder was the most effective treatment group at reducing PA counts. Silver oxynitrate 10% formulation had a high percentage of bacterial reduction. On day 7, compared to untreated control the silver oxynitrate 4, 7 and 10% treatments showed a 3.45, 4.05, and 4.30 log CFU/ml reduction, respectively. Conclusions These studies suggest that the silver oxynitrate formulations can reduce the bacterial bioburden in vivo against wounds that have PA biofilms. Additional animals are needed to substantiate these findings. Applicability of Research to Practice Gram-negative bacteria such as PA pose a challenge for wound care practitioners and new effective therapies are needed.

scholarly journals Bioshell Calcium Oxide (BiSCaO) Ointment for the Disinfection and Healing of Pseudomonas aeruginosa-Infected Wounds in Hairless Rats

2020 ◽  
Vol 21 (11) ◽  
pp. 4176 ◽  
Author(s):  
Tomohiro Takayama ◽  
Masayuki Ishihara ◽  
Shingo Nakamura ◽  
Yoko Sato ◽  
Sumiyo Hiruma ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Wound Healing ◽  
Calcium Oxide ◽  
Wound Repair ◽  
Chronic Wounds ◽  
Povidone Iodine ◽  
Bacterial Counts ◽  
Infected Wounds ◽  
And Control

Bioshell calcium oxide (BiSCaO) possesses deodorizing properties and broad microbicidal activity. This study aimed to investigate the application of BiSCaO ointment for the prevention and treatment of infection in chronic wounds in healing-impaired patients, without delaying wound healing. The bactericidal activities of 0.04, 0.2, 1, and 5 wt% BiSCaO ointment, 3 wt% povidone iodine ointment, and control (ointment only) were compared to evaluate the in vivo disinfection and healing of Pseudomonas aeruginosa-infected wounds in hairless rats. Treatment of the infected wounds with 0.2 wt% BiSCaO ointment daily for 3 days significantly enhanced wound healing and reduced the in vivo bacterial counts compared with povidone iodine ointment and control (no wound cleaning). Although 5 wt% BiSCaO ointment provided the lowest bacterial counts during 3 days’ treatment, it delayed wound healing. Histological examinations showed significantly advanced granulation tissue and capillary formation in wounds treated with 0.2 wt% BiSCaO ointment for 3 days compared to wounds treated with the other ointments. This study suggested that using 0.2 wt% BiSCaO ointment as a disinfectant for infected wounds and limiting disinfection to 3 days may be sufficient to avoid the negative effects of BiSCaO on wound repair.

scholarly journals ROS-Degradable Polythioketal Urethane Foam Dressings to Promote Porcine Skin Wound Repair

2021 ◽  
Author(s):  
Prarthana Patil ◽  
Katherine A Russo ◽  
Joshua T McCune ◽  
Alonda C Pollins ◽  
Matthew A Cottom ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Repair ◽  
Chronic Wounds ◽  
Degradation Products ◽  
Skin Wound ◽  
Foreign Body Response ◽  
Skin Wound Healing ◽  
Tissue Integration ◽  
Wound Matrix

Impaired skin healing and progression into chronic wounds is a prevalent and growing medical problem. Porous, resorbable biomaterials can be used as temporary substrates placed into skin defects to support cell infiltration, neo-tissue formation, and remodeling of nonhealing wounds. Naturally-derived biomaterials have promising healing benefits, but their low mechanical properties and exuberant costs limit their performance and use. Synthetic materials can be affordably manufactured and tuned across a broader range of physiochemical properties, but opportunities remain for tailoring them for ideal host immune and regenerative responses. Polyesters are the most clinically-tested class of synthetic biomaterials, but their hydrolysis releases acidic degradation products that can cause autocatalytic degradation processes that are poorly controlled and are not tied to cellular or other biologic activities. Here, we systemically explored a series of ROS-degradable polythioketal (PTK) urethane (UR) foams with varied hydrophilicity as an alternative class of synthetic biomaterials for wound healing. It was found that the most hydrophilic PTK-UR variant, which had 7 ethylene glycol (EG7) repeats flanking each side of each thioketal bond, had the highest ROS reactivity of the PTK-URs tested. In an in vivo porcine excisional skin wound healing model, hydrophilic EG7 PTK-UR foams more effectively promoted tissue integration, ECM deposition, and re-epithelialization of full-thickness skin wound compared to more hydrophobic PTK-UR variants. Resolution of type 1 inflammation and lower foreign body response to scaffold remnants was also observed for EG7 versus more hydrophobic PTK-UR scaffolds. Finally, porcine wound healing studies showed that EG7 PTK-UR foams had similar wound healing response to a collagen-based clinical gold standard product, Integra Bilayer Wound Matrix (BWM), while outperforming polyester UR foam-based NovoSorb Biodegradable Temporizing Matrix (BTM) with respect to increased ECM production, vascularization, and biomaterial-associated immune phenotype. In sum, PTK-UR foams warrant further development toward a new class of synthetic biomaterial foams for skin wound healing applications.

scholarly journals Hydrogel Preparation Methods and Biomaterials for Wound Dressing

Life ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 1016
Author(s):  
Jingjing Su ◽  
Jiankang Li ◽  
Jiaheng Liang ◽  
Kun Zhang ◽  
Jingan Li
Keyword(s):  
Wound Dressing ◽  
Wound Repair ◽  
Chronic Wounds ◽  
Healing Process ◽  
3D Structure ◽  
Skin Wound ◽  
Wound Dressings ◽  
Wound Healing Process ◽  
Skin Wound Healing ◽  
Preparation Methods

Wounds have become one of the causes of death worldwide. The metabolic disorder of the wound microenvironment can lead to a series of serious symptoms, especially chronic wounds that bring great pain to patients, and there is currently no effective and widely used wound dressing. Therefore, it is important to develop new multifunctional wound dressings. Hydrogel is an ideal dressing candidate because of its 3D structure, good permeability, excellent biocompatibility, and ability to provide a moist environment for wound repair, which overcomes the shortcomings of traditional dressings. This article first briefly introduces the skin wound healing process, then the preparation methods of hydrogel dressings and the characteristics of hydrogel wound dressings made of natural biomaterials and synthetic materials are introduced. Finally, the development prospects and challenges of hydrogel wound dressings are discussed.

Radiolabeled r-Hirudin as a Measure of Thrombin Activity at, or within, the Rabbit Aorta Wall In Vitro and In Vivo

1994 ◽  
Vol 71 (04) ◽  
pp. 499-506 ◽  
Author(s):  
Mark W C Hatton ◽  
Bonnie Ross-Ouellet
Keyword(s):  
Rabbit Aorta ◽  
Balloon Injury ◽  
Recombinant Hirudin ◽  
Aorta Wall ◽  
Thrombin Activity ◽  
Before And After ◽  
The Matrix

SummaryThe behavior of 125I-labeled recombinant hirudin towards the uninjured and de-endothelialized rabbit aorta wall has been studied in vitro and in vivo to determine its usefulness as an indicator of thrombin activity associated with the aorta wall. Thrombin adsorbed to either sulfopropyl-Sephadex or heparin-Sepharose bound >95% of 125I-r-hirudin and the complex remained bound to the matrix. Binding of 125I-r-hirudin to the exposed aorta subendothelium (intima-media) in vitro was increased substantially if the tissue was pre-treated with thrombin; the quantity of l25I-r-hirudin bound to the de-endothelialized intima-media (i.e. balloon-injured in vitro) correlated positively with the quantity of bound 131I-thrombin (p <0.01). Aortas balloon-injured in vivo were measured for thrombin release from, and binding of 125I-r-hirudin to, the de-endothelialized intimal surface in vitro; 125I-r-hirudin binding correlated with the amount of active thrombin released (p <0.001). Uptake of 125I-r-hirudin by the aorta wall in vivo was proportional to the uptake of 131I-fibrinogen (as an indicator of thrombin activity) before and after balloon injury. After 30 min in the circulation, specific 125I-r-hirudin binding to the uninjured and de-endo- thelialized (at 1.5 h after injury) aorta wall was equivalent to 3.4 (± 2.5) and 25.6 (±18.1) fmol of thrombin/cm2 of intima-media, respectively. Possibly, only hirudin-accessible, glycosaminoglycan-bound thrombin is measured in this way.

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