Chitosan & Conductive PANI/Chitosan Composite Nanofibers - Evaluation of Antibacterial Properties

Background: Within the healthcare industry, including the care of chronic wounds, the challenge of antimicrobial resistance continues to grow. As such, there is a need to develop new treatments that can reduce the bioburden in wounds. Objective: The present study is focused on the development of polyaniline (PANI) / chitosan (CH) nanofibrous electrospun membranes and evaluates their antibacterial properties. Methods: To this end, experimental design was used to determine the electrospinning windows of both pure chitosan and PANI/CH blends of different ratios (1:3, 3:5, 1:1). The effect of key environmental and process parameters (relative humidity and applied voltage) was determined, as well as the effect of the PANI/CH ratio in the blend and the molecular interactions between PANI and chitosan that led to jet stability. Results: The nanofibrous mats were evaluated regarding their morphology and antibacterial effect against model gram positive and gram negative bacterial strains, namely B. subtilis and E. coli. High PANI content mats show increased bactericidal activity against both bacterial strains. Conclusion: The blend fibre membranes combine the materials’ respective properties, namely electrical conductivity, biocompatibility and antibacterial activity. This study suggests that electrospun PANI/CH membranes are promising candidates for healthcare applications, such as wound dressings.

Download Full-text

Collagen-Chitosan Composite Powder as a Novel Biomaterial for Chronic Wounds in Hansen Disease-A Clinical Study

Journal of Pharmaceutical Research International ◽

10.9734/jpri/2021/v33i49b33352 ◽

2021 ◽

pp. 154-165

Author(s):

Pombala Suresh Babu ◽

Harini Srinivasan ◽

B. Sai Dhandapani ◽

C. Rose

Keyword(s):

Wound Repair ◽

Composite Powder ◽

Chronic Wounds ◽

Waste Product ◽

Wound Dressings ◽

Meat Industry ◽

Before And After ◽

Hematoxylin And Eosin ◽

Chitosan Composite

Aims: Natural biomaterials are more suitable than synthetic biomaterials for in vivo applications for treating damaged tissues. Collagen and chitosan are abundantly available natural biomaterials for wound dressings for tissue/wound repair. In this context, collagen-chitosan composite powder has been used to treat chronic wounds in Hansen disease (HD) patients. Place and Duration of Study: CSIR Central Leather Research Institute, Sardar Patel Road, Adyar, Chennai 600021, Southern Railway Headquarters Hospital, Constable Road, Ayanavaram, Chennai 600023, and Gremaltes Hospital, India between June 2013 and July 2020. Methodology: Collagen extracted from bovine rumen, a waste product of meat industry, and a commercially available chitosan were prepared as a composite powder (COL/CS) and applied to chronic wounds in HD patients after debridement and the wound contours were measured by planimetry. Biochemical parameters in blood samples were periodically assessed. Histopathology of wound tissue with Hematoxylin and Eosin and Masson’s Trichrome staining was studied. Matrix Metalloproteinase-9 (MMP9) levels before and after treatment were estimated. Results: Wound healing of 64.2% was obtained with COL/CS treatment and formation of granulation cells was observed early. Hemogram studies have been reported in a regression model with 95% confidence intervals. Histopathology revealed dense collagen fibres and continuity of sub-epithelial layer on 8th day. MMP-9 levels showed collagen integrity after treatment. Conclusion: The novel biocompatible, biodegradable COL/CS wound dressing is a promising biomaterial for management of chronic wounds in Hansen disease patients.

Download Full-text

Decoding Antibacterial and Antibiofilm Properties of Cinnamon and Cardamom Essential Oils: A Combined Molecular Docking and Experimental Study

10.21203/rs.3.rs-955241/v1 ◽

2021 ◽

Author(s):

Elahe Pourkhosravani ◽

fatemeh dehghan nayeri ◽

Mitra Mohammadi Bazargani

Keyword(s):

Cell Attachment ◽

Antibacterial Properties ◽

Binding Mode ◽

Dilution Method ◽

Antibiofilm Activity ◽

Bacterial Strains ◽

Biofilm Growth ◽

Computational Docking ◽

E Coli ◽

Industrial Strains

Abstract This study sets out to compare the antibacterial and antibiofilm profiles of Ci/Ca EOs alone and in combination together against infectious bacterial strains. MIC assay was carried out to survey the effectiveness of prepared EOs by two-fold serial dilution method and MTT evaluation. Synergic antibacterial properties of EOs against target strains were studied by using checkerboard titration method. Biofilm growth and development were evaluated using CV and XTT reduction assays. Antibacterial activity was observed for EOs against both bacterial strains with stronger activity for CiEO against both bacteria. The synergistic antibacterial effect was observed only against B. subtilis. Based on the FIC index, combinations could not inhibit the growth of E. coli. The pure EOs and their combination inhibited cell attachment for both studied bacteria with stronger effect on E. coli. CV and XTT reduction assays results showed that Ci EO and its combination with CaEO had the highest antibiofilm activity at lowest MIC value 0.08% and 0.04/0.02% against biofilm formed by E. coli and B. subtilis respectively, indicating a high antibiofilm potential. Computational docking analyses also postulated that the active constituents of evaluated EOs have the potential to interact with different bacterial targets, suggested binding mode of action of EOs metabolites. By and large, synergistic anti-biofilm properties of EOs may provide further options for developing novel formula to inhibit a variety of infectious clinical and industrial strains without (or less) toxicity effects on human body.

Download Full-text

Phytochemical Study and Antibacterial and Antibiotic Modulation Activity of Punica granatum (Pomegranate) Leaves

Scientifica ◽

10.1155/2020/8271203 ◽

2020 ◽

Vol 2020 ◽

pp. 1-7

Author(s):

Amine Trabelsi ◽

Mohamed Amine El Kaibi ◽

Aïmen Abbassi ◽

Amira Horchani ◽

Leila Chekir-Ghedira ◽

...

Keyword(s):

Antibacterial Properties ◽

Punica Granatum ◽

Diffusion Method ◽

Bacterial Strains ◽

Leaf Extracts ◽

Phytochemical Study ◽

E Coli ◽

Microdilution Method ◽

Resistant Strains

This study aimed to determine phytochemical contents, antibacterial properties, and antibiotic modulating potential of Punica granatum leaf extracts: hexane, chloroform, ethyl acetate, ethanol, and aqueous extracts as well as an extract enriched with total oligomer flavonoids (TOFs). The TOF extract contained the highest value of phenols and flavonoids. Rutin, luteolin, gallic acid, and ellagic acid were determined by HPLC analysis of this extract. The antibacterial activity was assayed by the disc diffusion method and microdilution method against Staphylococcus aureus and Escherichia coli standard ATCC strains and clinical isolates resistant strains. The TOF extract was the most active against all tested strains. The checkerboard method was used for the determination of synergy between two antibiotics (amoxicillin and cefotaxime) and P. granatum leaf extracts. The best synergistic interaction was found with TOF extract combined with amoxicillin for penicillin-resistant E. coli and penicillin-resistant S. aureus. These results can be assigned to tannins, flavonoids, and phenolic acids found in P. granatum leaf extracts. Pomegranate leaf extracts or active compounds isolated from these extracts could be used to fight the emergence and spread of resistant bacterial strains.

Download Full-text

Antibacterial-Integrated Collagen Wound Dressing for Diabetes-Related Foot Ulcers: An Evidence-Based Review of Clinical Studies

Polymers ◽

10.3390/polym12092168 ◽

2020 ◽

Vol 12 (9) ◽

pp. 2168

Author(s):

Ibrahim N. Amirrah ◽

Mohd Farhanulhakim Mohd Razip Wee ◽

Yasuhiko Tabata ◽

Ruszymah Bt Hj Idrus ◽

Abid Nordin ◽

...

Keyword(s):

Wound Healing ◽

Clinical Trials ◽

Clinical Studies ◽

Wound Dressing ◽

Chronic Wounds ◽

Gentian Violet ◽

Antibacterial Properties ◽

Small Sample ◽

Wound Dressings ◽

Sample Sizes

Diabetic foot ulcer (DFU) is a chronic wound frequently delayed from severe infection. Wound dressing provides an essential barrier between the ulcer and the external environment. This review aimed to analyse the effectiveness of antibacterial collagen-based dressing for DFU treatment in a clinical setting. An electronic search in four databases, namely, Scopus, PubMed, Ovid MEDLINE(R), and ISI Web of Science, was performed to obtain relevant articles published within the last ten years. The published studies were included if they reported evidence of (1) collagen-based antibacterial dressing or (2) wound healing for diabetic ulcers, and (3) were written in English. Both randomised and non-randomised clinical trials were included. The search for relevant clinical studies (n) identified eight related references discussing the effectiveness of collagen-based antibacterial wound dressings for DFU comprising collagen impregnated with polyhexamethylene biguanide (n = 2), gentamicin (n = 3), combined-cellulose and silver (n = 1), gentian violet/methylene blue mixed (n = 1), and silver (n = 1). The clinical data were limited by small sample sizes and multiple aetiologies of chronic wounds. The evidence was not robust enough for a conclusive statement, although most of the studies reported positive outcomes for the use of collagen dressings loaded with antibacterial properties for DFU wound healing. This study emphasises the importance of having standardised clinical trials, larger sample sizes, and accurate reporting for reliable statistical evidence confirming DFU treatment efficiency.

Download Full-text

Comparative Study of Physicochemical and Antibacterial Properties of ZnO Nanoparticles Prepared by Laser Ablation of Zn Target in Water and Air

10.20944/preprints201812.0125.v1 ◽

2018 ◽

Cited By ~ 1

Author(s):

Ekaterina A. Gavrilenko ◽

Daria A. Goncharova ◽

Ivan N. Lapin ◽

Anna L. Nemoikina ◽

Valery A. Svetlichnyi ◽

...

Keyword(s):

Lactic Acid ◽

Zno Nanoparticles ◽

Biomedical Applications ◽

Antibacterial Properties ◽

Pulsed Laser ◽

Wound Dressings ◽

E Coli ◽

Nanosecond Pulsed Laser ◽

Bactericidal Properties ◽

Biodegradable Poly

Here, we report on ZnO nanoparticles (NPs) generated by nanosecond pulsed laser (Nd:YAG, 1064 nm) through ablation of metallic Zn target in water and air and their comparative analysis as potential nanomaterials for biomedical applications. The prepared nanomaterials were carefully characterized in terms of their structure, composition, morphology and defects. It was found that in addition to the main wurtzite ZnO phase, which is conventionally prepared and reported by others, the sample laser-generated in air also contained some amount of monoclinic zinc hydroxynitrate. Both nanomaterials were then used to modify model wound dressings based on biodegradable poly-L-lactic acid. The as-prepared model dressings were tested as biomedical materials with bactericidal properties towards S. aureus and E. coli strains. The advantages of the NPs prepared in air over their counterparts generated in water found in this work are discussed.   

Download Full-text

Antibacterial effect of TiO2 modified with poly-amidoamine dendrimer – G3 on S. aureus and E. coli in aqueous solutions

Water Science & Technology ◽

10.2166/wst.2022.007 ◽

2022 ◽

Author(s):

Soudabeh Alizadeh Matboo ◽

Shahram Nazari ◽

Ali Niapour ◽

Mehdi Vosoughi Niri ◽

Esrafil Asgari ◽

...

Keyword(s):

Inhibitory Concentration ◽

Antibacterial Properties ◽

Plate Count ◽

Bacterial Strains ◽

Solution Ph ◽

E Coli ◽

Bacterial Removal ◽

Mic Minimum Inhibitory Concentration ◽

Paper Disc ◽

Surface Plate

Abstract This study investigated the bacterial removal using TiO2 nanoparticles (NPs) modified with poly-amidoamine dendrimer macromolecule (PAMAM, G3). The PAMAM G3/TiO2 (nanohybrid) was used to specify antibacterial properties via broth microdilution (MBC-Minimum Bactericidal Concentration and MIC-Minimum Inhibitory Concentration- determination), paper disc diffusion, and surface plate count methods. The nanohybrid was characterized via the different techniques. The effects of different factors including initial bacteria count, run time, solution pH, and the nanohybrid concentration were studied. The nanohybrid cytotoxicity was studied on AGS and MKN45 cells line by MTT assay. It was revealed that the nanohybrid was effective in intercepting both bacterial strains growth. The MIC value for S. aureus and E. coli were determined to be 4 and 2 μg/mL, respectively. The MBC value for both strains were calculated to be 32 μg/mL. The results showed removal efficiency of 100% for S. aureus and E. coli bacteria in optimum situation. The decrease in cell viability in the dosage of 32 μg/mL after 72 h treatment for AGS and MKN45 cells line were shown to be 6.2 and 4.6%, respectively. The nanohybrid was able to decrease the S. aureus and E. coli count in solution, which meets the drinking water criterions aligned with WHO guidelines.

Download Full-text

Comparative Study of Physicochemical and Antibacterial Properties of ZnO Nanoparticles Prepared by Laser Ablation of Zn Target in Water and Air

Materials ◽

10.3390/ma12010186 ◽

2019 ◽

Vol 12 (1) ◽

pp. 186 ◽

Cited By ~ 23

Author(s):

Ekaterina A. Gavrilenko ◽

Daria A. Goncharova ◽

Ivan N. Lapin ◽

Anna L. Nemoykina ◽

Valery A. Svetlichnyi ◽

...

Keyword(s):

Lactic Acid ◽

Zno Nanoparticles ◽

Biomedical Applications ◽

Antibacterial Properties ◽

Pulsed Laser ◽

Wound Dressings ◽

E Coli ◽

Nanosecond Pulsed Laser ◽

Bactericidal Properties ◽

Biodegradable Poly

Here, we report on ZnO nanoparticles (NPs) generated by nanosecond pulsed laser (Nd:YAG, 1064 nm) through ablation of metallic Zn target in water and air and their comparative analysis as potential nanomaterials for biomedical applications. The prepared nanomaterials were carefully characterized in terms of their structure, composition, morphology and defects. It was found that in addition to the main wurtzite ZnO phase, which is conventionally prepared and reported by others, the sample laser generated in air also contained some amount of monoclinic zinc hydroxynitrate. Both nanomaterials were then used to modify model wound dressings based on biodegradable poly l-lactic acid. The as-prepared model dressings were tested as biomedical materials with bactericidal properties towards S. aureus and E. coli strains. The advantages of the NPs prepared in air over their counterparts generated in water found in this work are discussed.

Download Full-text

Synergistic Effect of Chitosan and Selenium Nanoparticles on Biodegradation and Antibacterial Properties of Collagenous Scaffolds Designed for Infected Burn Wounds

Nanomaterials ◽

10.3390/nano10101971 ◽

2020 ◽

Vol 10 (10) ◽

pp. 1971

Author(s):

Jana Dorazilová ◽

Johana Muchová ◽

Kristýna Šmerková ◽

Silvia Kočiová ◽

Pavel Diviš ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Agents ◽

Chronic Wounds ◽

Porous Scaffold ◽

Antibacterial Properties ◽

Selenium Nanoparticles ◽

Dependent Manner ◽

Bacterial Strains ◽

Desirable Outcome ◽

The Stability

A highly porous scaffold is a desirable outcome in the field of tissue engineering. The porous structure mediates water-retaining properties that ensure good nutrient transportation as well as creates a suitable environment for cells. In this study, porous antibacterial collagenous scaffolds containing chitosan and selenium nanoparticles (SeNPs) as antibacterial agents were studied. The addition of antibacterial agents increased the application potential of the material for infected and chronic wounds. The morphology, swelling, biodegradation, and antibacterial activity of collagen-based scaffolds were characterized systematically to investigate the overall impact of the antibacterial additives. The additives visibly influenced the morphology, water-retaining properties as well as the stability of the materials in the presence of collagenase enzymes. Even at concentrations as low as 5 ppm of SeNPs, modified polymeric scaffolds showed considerable inhibition activity towards Gram-positive bacterial strains such as Staphylococcus aureus and methicillin-resistant Staphylococcus aureus and Staphylococcus epidermidis in a dose-dependent manner.

Download Full-text

Preparation of Electrospun Gelatin Mat with Incorporated Zinc Oxide/Graphene Oxide and Its Antibacterial Activity

Molecules ◽

10.3390/molecules25051043 ◽

2020 ◽

Vol 25 (5) ◽

pp. 1043 ◽

Cited By ~ 1

Author(s):

Honghai Li ◽

Yu Chen ◽

Weipeng Lu ◽

Yisheng Xu ◽

Yanchuan Guo ◽

...

Keyword(s):

Zinc Oxide ◽

Graphene Oxide ◽

Antibacterial Properties ◽

Degradation Process ◽

Antimicrobial Activities ◽

Wound Dressings ◽

X Ray ◽

E Coli ◽

Potential Applicability ◽

Electrospun Gelatin

Current wound dressings have poor antimicrobial activities and are difficult to degrade. Therefore, biodegradable and antibacterial dressings are urgently needed. In this article, we used the hydrothermal method and side-by-side electrospinning technology to prepare a gelatin mat with incorporated zinc oxide/graphene oxide (ZnO/GO) nanocomposites. The resultant fibers were characterized by field emission environment scanning electron microscopy (FESEM), energy-dispersive X-ray spectroscopy (EDX), X-ray diffractometry (XRD) and Fourier transform infrared spectroscopy (FTIR). Results indicated that the gelatin fibers had good morphology, and ZnO/GO nanocomposites were uniformly dispersed on the fibers. The loss of Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) viability were observed to more than 90% with the incorporation of ZnO/GO. The degradation process showed that the composite fibers completely degraded within 7 days and had good controllable degradation characteristics. This study demonstrated the potential applicability of ZnO/GO-gelatin mats with excellent antibacterial properties as wound dressing material.

Download Full-text

Atmospheric Pressure Microplasma for Antibacterial Silver Nanoparticle/Chitosan Nanocomposites With Tailored Properties

10.26434/chemrxiv.9849806.v1 ◽

2019 ◽

Author(s):

Daye Sun ◽

Jonathan Turner ◽

Nan Jiang ◽

Songsong Zhu ◽

Li Zhang ◽

...

Keyword(s):

Silver Nanoparticle ◽

Atmospheric Pressure ◽

Room Temperature ◽

Polymer Network ◽

Antibacterial Properties ◽

Bacterial Strains ◽

E Coli ◽

Modified Chitosan ◽

First Time

Room temperature atmospheric pressure microplasma (APM) was deployed for the first time for the in situ synthesis of anti-bacterial silver nanoparticle / chitosan (AgNP/CS) nanocomposites. The plasma induced liquid chemistry plays a role in the in situ formation of AgNP, the size distribution of which depends on the silver salt precursor concentration. The microplasma process has also simultaneously tailored the physical properties of the composites, rendering more crosslinked chitosan polymer network with shorter molecular chains. The formation of AgNP within the in situ modified chitosan has led to nanocomposites with overall improved mechanical properties and better stability in simulated body fluid. Our plasma synthesized AgNP/CS nanocomposites also demonstrate effective antibacterial properties against E. Coli and S. Aureus bacterial strains, showing their promise in potential antimicrobial applications.

Download Full-text