Modern lineages of Mycobacterium tuberculosis were recently introduced in western India and demonstrate increased transmissibility

Background: Mycobacterium tuberculosis (Mtb) transmissibility may vary between lineages (or variants) and this may contribute to the slow decline of tuberculosis (TB) incidence. The objective of our study was to compare transmissibility across four major lineages (L1-4) of Mtb among participants from two cohort studies in Pune, India. Methods: We performed whole-genome sequencing (WGS) of Mtb sputum culture-positive isolates from participants in two prospective cohort studies of adults with pulmonary TB seeking care at public treatment centers in Pune, Maharashtra. We performed genotypic susceptibility prediction for both first- and second-line drugs using a previously validated random forest model. We used single nucleotide substitutions (SNS) and maximum likelihood estimation to build isolate phylogenies by lineage. We used Bayesian molecular dating to estimate ancestral node ages and compared tree characteristics using a two-sample Kolmogorov-Smirnov (KS) test. Results: Of the 642 isolates from distinct study participants that underwent WGS, 612 met sequence quality criteria. The median age of the 612 participants was 31 years (IQR 24.4-44.2), the majority were male (64.7%) and sputum smear-positive (83.3%), and 6.7% had co-infection with HIV. Most isolates belonged to L3 (44.6%). The majority (61.1%) of multidrug-resistant isolates (MDR, resistant to isoniazid and rifampin) belonged to L2 (P < 0.001 [Fisher's Exact]). There was no significant difference in host characteristics between participants infected with the four major lineages. In phylogenetic analysis, we measured shorter terminal branch lengths in the L2 tree compared to L1 and L3 trees indicating less time elapsing between transmission and sampling and higher transmissibility (median branch lengths: L2 - 3.3, L3 - 7.8, p <0.001). Branching times for L2 and L4 were more recent than L1 and L3 indicating recent introduction into the region (p < 0.01 [KS test]). Conclusion: Modern Mtb lineages (L2 and L4) were more recently introduced in western India, compared to older lineages (L1 and L3). L2 shows a higher frequency of drug-resistance and higher transmissibility. Our findings highlight the need for contact tracing around cases of TB due to L2, and heightened surveillance of TB antibiotic resistance in India.

A Novel Pipeline Leak Detection Technique Based on Acoustic Emission Features and Two-Sample Kolmogorov–Smirnov Test

Pipeline leakage remains a challenge in various industries. Acoustic emission (AE) technology has recently shown great potential for leak diagnosis. Many AE features, such as root mean square (RMS), peak value, standard deviation, mean value, and entropy, have been suggested to detect leaks. However, background noise in AE signals makes these features ineffective. The present paper proposes a pipeline leak detection technique based on acoustic emission event (AEE) features and a Kolmogorov–Smirnov (KS) test. The AEE features, namely, peak amplitude, energy, rise-time, decay time, and counts, are inherent properties of AE signals and therefore more suitable for recognizing leak attributes. Surprisingly, the AEE features have received negligible attention. According to the proposed technique, the AEE features are first extracted from the AE signals. For this purpose, a sliding window was used with an adaptive threshold so that the properties of both burst- and continuous-type emissions can be retained. The AEE features form distribution that change its shape when the pipeline condition changes from normal to leakage. The AEE feature distributions for leak and healthy conditions were discriminated using the two-sample KS test, and a pipeline leak indicator (PLI) was obtained. The experimental results demonstrate that the developed PLI accurately distinguishes the leak and no-leak conditions without any prior leak information and it performs better than the traditional features such as mean, variance, RMS, and kurtosis.

1397. Modern Lineages of Mycobacterium tuberculosis Were Recently Introduced in Western India and Demonstrate Increased Transmissibility

Background Mycobacterium tuberculosis (Mtb) transmissibility may vary between lineages (or variants) and this may contribute to the slow decline of tuberculosis incidence. The objective of our study was to compare transmissibility across four major lineages (L1-4) of Mtb in Pune, India. Methods We performed whole-genome sequencing (WGS) of Mtb isolated from sputum culture of adult patients with pulmonary TB. We performed genotypic susceptibility testing for both first- and second-line drugs using a previously validated random forest predictor. We identified single nucleotide polymorphisms and generated a multiple sequence alignment excluding drug resistance conferring mutations to avoid skewing the phylogeny due to convergent evolution in these regions. We used Bayesian molecular dating to generate phylogenies and compared tree characteristics using a two-sample Kolmogorov-Smirnov (KS) test. Results Of the 642 isolates from distinct study participants that underwent WGS, 612 met quality criteria. The median age of participants was 31 years (range 18-74), the majority were male (64.7%) and sputum smear-positive (83.3%), and 6.7% had co-infection with HIV (Table 1). There was no significant difference in baseline characteristics between lineages. The majority of isolates belonged to L3 (44.6%). The majority (61.1%) of multidrug-resistant (MDR, resistant to isoniazid and rifampin) isolates belonged to L2. In phylogenetic analysis, we found evidence of higher transmissibility of L2 as indicated by shorter branch lengths (i.e., less time had elapsed between transmission and sampling) and more genetic similarity (smaller pairwise single nucleotide polymorphism [SNP] distances) among L2 isolates as compared to other lineages (Figure 1). Branching times for L2 and L4 were smaller than L1 and L3 indicating recent introduction into the region (p &lt; 0.001 [KS test]). Figure 1: Lineage-wise distribution of A) phylogenetic tree branch lengths (log) and B) pairwise single nucleotide polymorphism (SNP) distance, using 612 tuberculosis isolates from Pune, India. P values calculated using two-sample Kolmogorov-Smirnov test. Table 1: Demographic characteristics of study participants included in the study, by lineage. Conclusion Modern Mtb lineages (L2 and L4) were relatively recently introduced in western India, as compared to older lineages (L1 and L3), with the more drug-resistant L2 showing higher transmissibility. These findings highlight the need for early detection and treatment initiation to interrupt transmission with important implications for antimicrobial stewardship and heightened surveillance of TB resistance rates. Disclosures All Authors: No reported disclosures

Subdivided Cellular Automata Model Considering Anticipation Floor Field and Analysis of Pedestrian Detour Behavior

The micro-pedestrian simulation model represented by the cellular automata model is an important simulation model. Improvements in various aspects enable a better description of the various behaviors of pedestrians, such as pedestrian avoidance behavior, companion behavior, as well as transcendence behavior, waiting behavior and detour behavior. This paper takes the pedestrian detour behavior in the circle antipode experiment as the main entry point. The subdivision cellular automaton model is integrated into the prediction field to model and simulate the detour behavior. At the same time, it explores the degree of subdivision of the cell. Pedestrian heterogeneity and the influence of predicted field potential energy on the simulated pedestrian trajectory. Finally, based on the temporal and spatial indicators of pedestrian trajectory characteristics, the KS test and DTW method are used to evaluate the similarity of the trajectory distribution characteristics and time series characteristics with experimental samples, and evaluate and compare models with or without heterogeneity. The results show that the trajectory characteristics of heterogeneous pedestrians are closer to the experiment than homogeneous pedestrians.

Is the Configuration of Indian Stock Market Weakly Efficient?

The main aim of this study is to find out the whether the Indian stock market efficiency is in weak form. The aim of this study is to look into the Indian Stock Market’s lack of market performance. From 2000 to 2015, sample is gathered on a daily, weekly, and monthly basis. Unit Root Test, Run Test, and KS Test are used to examine the data. According to the findings, The Runs Test disproves the existence of a random walk and demonstrates that the Indian stock market is not weakly efficient. Through stock valuation strategies, technical and fundamental analysts may generate volatile returns.

Supplemental Material: Quantifying structural controls on submarine channel architecture and kinematics

Figure S1: Location of cross sections through Channel Complex Five; Figure S2: Location of cross-sections taken through Channel Complex Six; Figure S3: (a) The final channel elements of channel complex five (ChC 5) and six (ChC 6) overlain on interval strain rates presented in Pizzi et al. (2020); Table S1: KS test values for channel complex five and six. Critical values based on the two sample sizes (structured (m) and unstructured (n) measurements) and Dmax values for channel complex width, thickness, aspect ratio, and complex-averaged stratigraphic mobility.

Supplemental Material: Quantifying structural controls on submarine channel architecture and kinematics

Figure S1: Location of cross sections through Channel Complex Five; Figure S2: Location of cross-sections taken through Channel Complex Six; Figure S3: (a) The final channel elements of channel complex five (ChC 5) and six (ChC 6) overlain on interval strain rates presented in Pizzi et al. (2020); Table S1: KS test values for channel complex five and six. Critical values based on the two sample sizes (structured (m) and unstructured (n) measurements) and Dmax values for channel complex width, thickness, aspect ratio, and complex-averaged stratigraphic mobility.

784 Naturalistic Measurement of Sleep/Wake Disturbance in Adults Receiving Methadone Treatment for Opioid Use Disorder

Introduction Individuals with opioid use disorder (OUD) report significant sleep/wake disturbance, which continues even after stabilization on medication-assisted treatment (MAT). However, the nature of sleep/wake disturbance in this population has not been well documented objectively. Here we analyze naturalistic wrist actigraphy recordings in individuals with OUD receiving methadone-based MAT. Methods Seven adults undergoing methadone treatment for OUD (ages 26–50; 4 women) wore a wrist actigraph (Actiwatch-2, Philips Respironics) continuously for 7 days. They were asked to adhere to their normal sleep schedule in order to obtain naturalistic observations. Reference data were collected in a separate study of healthy controls, in which 14 hospital nurses (ages 20–60; 13 women) wore a wrist actigraph continuously for 7 days. In this reference group, 7 participants had a day shift schedule (07:00–19:00) and 7 had a night shift schedule (19:00–07:00), with six 12h shifts in a 2-week period. Actigraphic data were collected in 1min epochs, and the sleep/wake status for each epoch was estimated using Actiware 6.0.9 (Philips Respironics). The estimated sleep/wake patterns were subjected to cosinor analysis to assess 24h rhythmicity and analysis of the distribution of inactive periods to assess sleep continuity. Results For the reference group, nurses working day shifts displayed strong 24h rhythmicity, whereas nurses working night shifts showed blunted 24h rhythmicity (F[1,12]=66.11, p&lt;0.001). However, both day and night nurses exhibited high sleep continuity (KS test, p=0.82). By contrast, for the group with OUD receiving methadone, the strength of 24h rhythmicity was reduced to between that of the day and night shift nurses in the reference group, indicating weak regularity of sleep/wake patterns (F[2,18]=33.79, p&lt;0.001). Furthermore, individuals with OUD receiving methadone experienced low sleep continuity compared to the reference group (KS test, p=0.030). Conclusion These naturalistic observations confirm the presence of sleep/wake disturbance, resulting from both irregular sleep/wake patterns and low sleep continuity, in individuals receiving methadone-based MAT for OUD. Sleep/wake disturbance may interfere with the ability to achieve OUD recovery goals, and comparing sleep disturbance in MAT populations to reference data highlights the need to consider sleep in these populations as a clinical priority. Support (if any) State of Washington Initiative Measure No. 171

APPLICATION OF THE KOLMOGOROV-SMIRNOV TEST TO COMPARE GREENHOUSE GAS EMISSIONS OVER TIME

The national inventories of greenhouse gas (GHG) emissions, which are periodically prepared by countries that signed the Climate Change Convention, compute emissions from anthropogenic sources among them agricultural activities. The protocols established within the scope of the International Panel on Climate Change (IPCC) make it possible to estimate these emissions. These protocols use standard emission factors that vary according to the characteristics of the monitored activities and only scientific research, published in journals of recognized quality, can establish other local factors. Brazilian researchers carry out experiments to measure GHG emissions from agricultural activities, aiming to calculate specific parameters for the national climatic and management conditions. These field experiments are complex, costly, with a limited number of repetitions and, eventually, high natural variability. Often, these limitations result in the inability of the analysis of variance (ANOVA) to identify differences between treatments. The objective of this work is to present the non-parametric Kolmogorov-Smirnov (KS) test as an alternative to compare the effect of flooded irrigation management on methane (CH4) emission throughout the rice crop cycle. We present a case study in which ANOVA produced non-significant results for the adjustment of the model while the KS identified the emission curves as significantly different. The KS test could be adapted, via the SAS NPAR1WAY routine, to compare events with responses over time, such as methane emissions in flooded rice, resulting in test values and graphs that are easy to understand and interpret.

Variable Magellanic HMXB Sources versus Variable ULX Sources: Nothing to Brag about the ULX Sources

We carry out a meta-analysis of ultraluminous X-ray (ULX) sources that show large variabilities (by factors of >10) between their highest and lowest emission states in the X-ray energy range of 0.3–10 keV. We are guided by a recent stringent compilation of 25 such X-ray sources by Song et al. We examine the relation of logN versus logSmax, where N is the number of sources radiating above the maximum-flux level Smax. We find a strong deviation from all previously determined slopes in various high-mass X-ray binary (HMXB) samples. In fact, the ULX data clearly show a slope of −0.91. Thus, ULX sources do not appear to be uniform and isotropic in our Universe. We compare the ULX results against the local X-ray luminosity function of HMXBs in the Small Magellanic Cloud (SMC) constructed from our latest library that includes 41 Chandra 0.3–8 keV sources and 56 XMM-Newton 0.2–12 keV sources. The ULX data are not drawn from the same continuous distribution as the SMC data (the ULX data peak at the low tails of the SMC distributions), and none of our data sets is drawn from a normal distribution or from a log-normal distribution (they all show marked excesses at both tails). At a significance level of α=0.05 (2σ), the two-sample p-value of the Kolmogorov–Smirnov (KS) test gives p=4.7×10−3<α for the ULX versus the small Chandra sample and p=1.1×10−5<<α for the ULX versus the larger XMM-Newton sample, respectively. This adds to the evidence that ULX sources are not simply the higher end of the known local Be/X-ray pulsar distribution, but they represent a class of X-ray sources different from the young sources found in the SMC and in individual starburst galaxies. On the other hand, our two main SMC data sets are found to be statistically consistent, as they are drawn from the same continuous parent distribution (null hypothesis H0): at the α=0.05 significance level, the two-sample KS test shows an asymptotic p-value of 0.308>α, which tells us to accept H0.