Bidirectional flow of the funny current (If) during the pacemaking cycle in murine sinoatrial node myocytes

Sinoatrial node myocytes (SAMs) act as cardiac pacemaker cells by firing spontaneous action potentials (APs) that initiate each heartbeat. The funny current (If) is critical for the generation of these spontaneous APs; however, its precise role during the pacemaking cycle remains unresolved. Here, we used the AP-clamp technique to quantify If during the cardiac cycle in mouse SAMs. We found that If is persistently active throughout the sinoatrial AP, with surprisingly little voltage-dependent gating. As a consequence, it carries both inward and outward current around its reversal potential of −30 mV. Despite operating at only 2 to 5% of its maximal conductance, If carries a substantial fraction of both depolarizing and repolarizing net charge movement during the firing cycle. We also show that β-adrenergic receptor stimulation increases the percentage of net depolarizing charge moved by If, consistent with a contribution of If to the fight-or-flight increase in heart rate. These properties were confirmed by heterologously expressed HCN4 channels and by mathematical models of If. Modeling further suggested that the slow rates of activation and deactivation of the HCN4 isoform underlie the persistent activity of If during the sinoatrial AP. These results establish a new conceptual framework for the role of If in pacemaking, in which it operates at a very small fraction of maximal activation but nevertheless drives membrane potential oscillations in SAMs by providing substantial driving force in both inward and outward directions.

Bi-directional flow of the funny current (If) during the pacemaking cycle in murine sinoatrial node myocytes

Sinoatrial node myocytes (SAMs) act as cardiac pacemaker cells by firing spontaneous action potentials (APs) that initiate each heartbeat. The funny current, If, is critical for the generation of these spontaneous APs; however, its precise role during the pacemaking cycle remains unresolved. We used the AP-clamp technique to quantify If during the cardiac cycle in mouse SAMs. We found that If is persistently active throughout the sinoatrial AP, with surprisingly little voltage-dependent gating. As a consequence, it carries both inward and outward current around its reversal potential of -30 mV. Despite operating at only 2-5% of its maximal conductance, If carries a substantial fraction of both depolarizing and repolarizing net charge movement during the firing cycle. We also show that β-adrenergic receptor stimulation increases the percentage of net depolarizing charge moved by If, consistent with a contribution of If to the fight-or-flight increase in heart rate. These properties were confirmed by heterologously-expressed HCN4 channels and by mathematical models of If. Modelling further suggested that the slow activation and deactivation of the HCN4 isoform underlie the persistent activity of If during the sinoatrial AP. These results establish a new conceptual framework for the role of If in pacemaking, in which it operates at a very small fraction of maximal activation but nevertheless drives membrane potential oscillations in SAMs by providing substantial driving force in both inward and outward directions.Significance StatementCardiac pacemaker cells trigger each heartbeat by virtue of spontaneous oscillations in their membrane voltage. Although the funny current (If) is critical for these oscillations and for setting heart rate, its precise role remains an enigma because it activates mostly outside of the physiological voltage range and quite slowly relative to the pacemaker cycle. Here we show that If is persistently active in pacemaker cells; once opened, the small fraction of ion channels that conduct If do not re-close. Consequently, If flows both inward and outward to help propel the voltage oscillations and it paradoxically conducts a large fraction of the net charge movement. These results establish a new conceptual framework for the role of If in driving cardiac pacemaking.

Recoil Reduction Method of Gun with Side to Rear Jet Controlled by Piston Motion

Excessive recoil severely restricts the loading of high-power traditional guns on modern vehicles. To reduce the recoil without breaking the continuous firing mode and reducing the projectile velocity, a recoil reduction method that controls the lateral ejecting of propellant gas by a piston was proposed. The recoil reduction device is symmetric about the barrel axis. First, a one-dimensional two-phase flow model of interior ballistic during the gun firing cycle was established. Next, the MacCormack scheme was used to simulate, and the piston motion was gained. Then the propagation of the rarefaction wave in the barrel was presented. Finally, the propulsion difference between the piston-controlled gun and the traditional gun was discussed. The results showed that the recoil momentum was reduced by 31.80%, and the muzzle velocity was decreased by just 1.30% under the reasonable matching of structural parameters.

Influence of Surroundings on Radiant Tube Lifetime in Indirect-Fired Vertical Strip Annealing Furnaces

The effects of surrounding radiation—emanating from radiation exchange with neighboring partners in indirect-fired vertical strip annealing furnaces, such as the other radiant tubes, the passing strip, and the enclosing furnace chamber—on the radiant tube lifetime were studied. In-house developed and validated numerical models were used to calculate the thermomechanical behavior, especially creep deformations and the corresponding stresses as lifetime indicating parameters. Different setups of recirculating P-type radiant tubes were investigated, including a reference case of an isolated tube. The investigations could be broadly classified into the study of the effects of different tube arrangements, burner operations (synchronous/asynchronous on/off firing), and changes of strip parameters (width/temperature). Results showed higher creep deformation of the central radiant tube in the setup with three tubes arranged horizontally in a row compared to three tubes stacked in a vertical column, even though the respective characteristic temperature values in a firing cycle were similar. Furthermore, the cases with asynchronous burner firing resulted in lower creep rates than other cases, where the burners were operating in synchronous on/off firing modes. In addition, the change of strip width had a higher impact on radiant tube lifetime compared to locally changing strip temperatures across the furnace. Alternating temperatures, caused by burner operation or process changes, such as change of strip’s speed or cross-section, and local temperature gradients were observed to be the main factors influencing the tube’s service life.

In-Cylinder CO2 Sampling Using Skip-Firing Method

Skip-firing (or cylinder de-activation) was assessed as a method of sampling CO2 directly in the cylinder at higher speeds than previously possible. CO2 was directly sampled from one cylinder of a 1 L three-cylinder gasoline engine to determine the residual gas fraction (RGF) using a fast response CO/CO2 analyzer. Acquisition of data for similar measurements is typically limited to engine speeds of below 1300 revolutions per minute (rpm) to allow full resolution of the sample through the analyzer that has an 8 ms finite response time. In order to sample in-cylinder CO2 at higher engine speeds, a skip-firing method is developed. By shutting off ignition intermittently during engine operation, the residual CO2 from the last firing cycle can be measured at significantly higher engine speeds. Comparison of RGF CO2 at low speeds for normal and skip-fire operation shows good correlation. This suggests that skip-firing is a suitable method for directly measuring internal exhaust gas recirculation up to at least 3000 rpm. The measurements obtained may provide a useful tool for validating internal exhaust gas recirculation models and could be used to calculate combustion air–fuel ratio from the CO and CO2 content of the burned gas. These are typically complicated parameters to predict due to the slow response time and sensitivity to hydrocarbons of wide-band oxygen sensors. A differing pattern of RGF change with increasing speed was seen between normal and skip-fire operation.

In-Cylinder CO2 Sampling Using Skip-Firing Method

Skip-firing (or cylinder de-activation) was assessed as a method of sampling CO2 from directly in the cylinder at higher speeds than previously possible. CO2 was directly sampled from one cylinder of a 1-litre 3-cylinder gasoline engine to determine the residual gas fraction using a fast response CO/CO2 analyser. Acquisition of data for similar measurements is typically limited to engine speeds of below 1300 rpm to allow full resolution of the sample, through the analyser that has an 8 millisecond finite response time. In order to sample in-cylinder CO2 at higher engine speeds a skip-firing method is developed. By shutting off ignition intermittently during engine operation, the residual CO2 from the last firing cycle can be measured at significantly higher engine speeds. Comparison of residual gas fraction CO2 at low speeds for normal and skip-fire operation shows good correlation. This suggests that skip-firing is a suitable method for directly measuring internal exhaust gas recirculation up to at least 3000 rpm. The measurements obtained may provide a useful tool for validating internal exhaust gas recirculation models and could be used to calculate combustion air-fuel ratio from the CO and CO2 content of the burned gas. These are typically complicated parameters to predict due to the slow response time and sensitivity to hydrocarbons of wide-band oxygen sensors. A differing pattern of residual gas fraction change with increasing speed was seen between normal and skipfire operation.

Study on Valve Strategy and Fuel Benefits of Skip Fire Based on Electromagnetic Valve Train

Cylinder deactivation (CDA) is a fuel consumption reduction technology for gasoline engines. Skip fire is a new type of CDA because the load and the density of firing cylinder are in proportion to the torque demand. However, it is difficult to realize because valves need to be switched between valve deactivation and normal operation stroke by stroke. The Electromagnetic valve train (EMVT) provides a fully flexible control method to achieve skip fire. In the paper, a new skip fire strategy based on electromagnetic intake valve train (EMIV) is proposed. Then, the oxygen concentration of the exhaust pipe, energy losses, in-cylinder pressure of the skipped cycle and exhaust gas recirculation (EGR) rate of the firing cycle are studied by the 1D simulation in GT-Power. The results shows the majority of gas sucked into the skipped cylinder is exhaust gas by reasonable control of IVO and IVC, and the exhaust oxygen-rich can be avoided. Meanwhile, EGR rate of the firing cylinder and energy losses of the skipped cylinder are maintained at lower level. At the conditions of 1200 and 1600 rpm, fuel economy has been improved respectively 8.1%-16.6% and 6.4%-14.6% when the brake mean effective pressure (BMEP) ranges from 0.4MPa to 0.2MPa.