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Abstract The use of frozen semen lowers the risk of disease transmission, eliminates geographical limitations and supports the implementation of genetic resource protection programs. However, due to the very rare use of frozen semen from Hutsul stallions, their genetic material is not secured in sperm banks, and very little information is available about their semen, including its suitability for cryopreservation, and sperm survival rates after thawing. The aim of this study was to analyse basic parameters such as sperm motility, vitality and morphology in diluted-stored and post-thawed Hutsul semen, using a CASA system. There were no differences in sperm motility (P = 0.3372) or morphology between the groups, although the progressive motility was higher in thawed semen (P = 0.0151), while the sperm vitality was higher in diluted-stored semen (P = 0.00517). This study demonstrates that semen from Hutsul horses is suitable for cryopreservation, thus supporting the creation of a sperm bank as a genetic reserve for representatives of this breed.


Plants ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2383
Author(s):  
Xiaofeng Chi ◽  
Faqi Zhang ◽  
Qingbo Gao ◽  
Rui Xing ◽  
Shilong Chen

Gentianaceae is a large plant family and is distributed worldwide. As the largest tribe in Gentianaceae, Gentianeae contains 939–968 species, and the Qinghai-Tibet Plateau and adjacent areas are the main centers of diversity for Gentianeae. Species from the Gentianeae are widely used in traditional Tibetan medicine. In this review, a systematic and constructive overview of the information on botany, ethnomedicinal usage, phytochemistry, and pharmacological properties of Gentianeae in Tibetan medicine is provided. The results of this study are based on a literature search, including electronic databases, books, websites, papers, and conference proceedings. Botanical studies showed that Gentianeae includes the subtribe Gentianeae and Swertiinae, and several new genera and taxa have been identified. Approximately 83 species from Gentianeae were used in Tibetan medicine, among which Gentiana and Swertia constituted the largest number of species with 42 and 24 species, respectively. The species from Gentianeae are mainly used as Bangjian (སྤང་རྒྱན།), Jieji (ཀྱི་ལྕེ།), Dida (ཏིག་ཏ།), and Ganggaqiong (གང་གྰཆུང་།) in Tibetan medicine with different clinical applications. More than 240 formulas were found containing Gentianeae species with different attending functions. Phytochemical studies showed that the main active components of Gentianeae species are iridoids, xanthones, flavonoids, and triterpenoids. The bioactivities of plants from Gentianeae include hepatic protection, upper respiratory tract protection, joint and bone protection, glucose regulation, antibacterial, antioxidant, anticancer, and antiviral effects. This review will provide a reference for future research on natural resource protection, plant-based drug development, and further clinical investigation.


2021 ◽  
Vol 13 (21) ◽  
pp. 11875
Author(s):  
Yen-Chiang Chang ◽  
Mehran Idris Khan

In addition to the traditional so-called Arctic states, non-Artic states and some other international organisations are now showing a growing interest in this area. China, for example, has achieved some progress, since becoming an Arctic Council permanent observer, through participation in resource development in the region and strengthening its bilateral relations with the Arctic states. The present study examines China’s Arctic policy and its implications for the governance of the Arctic Ocean. It also provides an insight into the existing relevant international legal instruments and examines China’s interest in the participation, governance, and resource protection activities in the Arctic Ocean region, to successfully implement Chinese Arctic policy. To this end, the study examines the connection between the “white paper” and “China’s Arctic Policy”, in the context of executing the “Belt and Road Initiative”, in particular the “Arctic Silk Road”. The study concludes that China intends to perform a dynamic role in governing the Arctic Ocean, as a less challenging but cooperative partner in this region.


2021 ◽  
Vol 80 (21) ◽  
Author(s):  
Stefano Lo Russo ◽  
Enrico Suozzi ◽  
Martina Gizzi ◽  
Glenda Taddia

AbstractIt has become increasingly necessary to optimise mountain groundwater resource management and comprehend resource-recharging systems from a hydrogeological perspective to formulate adequate resource protection strategies. Analysing mountain spring behaviour and aquifer characteristics can be time-consuming, so new automated techniques and software tools are needed to estimate hydrogeological parameters and understand the exhaustion dynamics of groundwater resources. This paper introduces SOURCE, a new semi-automatic tool that automates the hydrogeological characterisation of water springs and provides proper estimations of the vulnerability index, as well as autocorrelation and cross-correlation statistical coefficients. SOURCE rapidly processed input data from the Mascognaz 1 spring (Aosta Valley) water probes and meteorological station to provide graphical outputs and values for the main hydrodynamic parameters. Having a single software package that contains all the main methods of water spring analysis could potentially reduce analysis times from a few days to a few hours.


2021 ◽  
pp. 0160323X2110382
Author(s):  
Francine S. Romero

While most U.S. cities have a tree protection policy, the subsequent impact on the reduction of canopy loss is unclear. To rectify this, I utilize a theoretically grounded framework of influence comprised of clear identification of the problem/public support, adequate resources, and sound policy logic. This is then tested in a comparative case study of Charlotte, North Carolina, and San Antonio, Texas. While Charlotte benefits from public recognition of the problem and adequate resources, its regulations are weak, lacking a logical connection to aspirational outcomes. San Antonio's regulations are stronger, but combined with weaker problem identification and resources. Through quantitative and qualitative assessments, I find that San Antonio's strict regulations may have stabilized loss rates, while Charlotte's weaker rules have not. Results highlight the importance of policy logic over other commonly suggested determinants of natural resource protection.


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