Periaqueductal gray neurons encode the sequential motor program in hunting behavior of mice

Sequential encoding of motor programs is essential for behavior generation. However, whether it is critical for instinctive behavior is still largely unknown. Mouse hunting behavior typically contains a sequential motor program, including the prey search, chase, attack, and consumption. Here, we reveal that the neuronal activity in the lateral periaqueductal gray (LPAG) follows a sequential pattern and is time-locked to different hunting actions. Optrode recordings and photoinhibition demonstrate that LPAGVgat neurons are required for the prey detection, chase and attack, while LPAGVglut2 neurons are selectively required for the attack. Ablation of inputs that could trigger hunting, including the central amygdala, the lateral hypothalamus, and the zona incerta, interrupts the activity sequence pattern and substantially impairs hunting actions. Therefore, our findings reveal that periaqueductal gray neuronal ensembles encode the sequential hunting motor program, which might provide a framework for decoding complex instinctive behaviors.

A persistent behavioral state enables sustained predation of humans by mosquitoes

Predatory animals first detect, then pursue, and ultimately capture prey. Sensory cues, including scent emitted by prey, are detected by the predator and used to guide pursuit. Because the pursuit phase can last for extended periods of time, it is critical for predators to persist in the chase even when prey is difficult to detect in a noisy sensory land-scape. It is equally important for predators to abandon pursuit if enough time has elapsed that prey capture is unlikely to occur. We studied prey detection and sustained pursuit in the mosquito Aedes aegypti, a micropredator of humans. These animals first detect hu-mans through sensory cues that are emitted at a distance such as carbon dioxide in breath and odor from skin. As the mosquito approaches a human, additional cues such as body heat and visual contrast signal the promise of a blood meal, which females need to produce eggs. To study how initial prey detection influences the duration of pursuit, we developed optogenetic tools to induce a brief fictive sensation of carbon dioxide and used machine learning-based classification of behavior to investigate how mosquitoes respond to subsequent human cues. We found that a 5-second optogenetic pulse of fictive carbon dioxide induced a persistent behavioral state in female mosquitoes that lasted for more than 10 minutes. This state is highly specific to females searching for a blood meal and was not induced in recently blood-fed females or in males, who do not feed on blood. In males that lack the gene fruitless, which controls persistent social behaviors in other insects, fictive carbon dioxide induced a long-lasting behavior response resembling the predatory state of females. Finally, we show that the persistent state triggered by detection of fictive carbon dioxide enabled females to engorge on a blood meal mimic offered up to 14 minutes after the initial stimulus. Our results demonstrate that a persistent internal state allows female mosquitoes to integrate multiple human sensory cues over long timescales, an ability that is key to their success as an apex micropredator of humans

Contributions to a Discussion of Spinosaurus aegyptiacus as a Capable Swimmer and Deep-Water Predator

The new findings on Spinosaurus’ swim tail strongly suggest that Spinosaurus was a specialized deep-water predator. However, the tail must be seen in the context of the propelled body. The comparison of the flow characteristics of Spinosaurus with geometrically similar animals and their swimming abilities under water must take their Reynolds numbers into account and provide a common context for the properties of Spinosaurus’ tail and dorsal sail. Head shape adaptations such as the head crest reduced hydrodynamic disturbance and facilitated stealthy advance, especially when hunting without visual contact, when Spinosaurus could have used its rostral integumentary mechanoreceptors for prey detection. The muscular neck permitted ‘pivot’ feeding, where the prey’s escape abilities were overcome by rapid dorsoventral head movement, facilitated by crest-mediated lower friction.

Electric Attraction

This chapter assesses the ability of animals to detect and interpret electric information. While sharks often use chemical information to track down prey from a long distance, many species enlist their electric sense to detect electric cues and determine the prey’s precise location and direct their attacks. Although it is normally used for prey detection, the electric sense can sometimes be used in defence too. The chapter then explores the diversity of ways electricity is produced and used by weakly electric fish. Meanwhile, the platypus can use their electric sense both to avoid objects in the water and to locate small prey items. The echidna also has receptors on the tip of its snout that respond to electric information, but its electric sense seems quite limited. Finally, the chapter considers how bees are able to detect electric fields associated with flowers.

Stars of the Tactile World

This chapter addresses the supreme level of refinement found in many animals for analysing tactile and pressure information. It begins by looking at the sensory organ of the star-nosed mole. The mole’s star-shaped organ is used purely for collecting tactile information. The chapter then considers the Eimer’s organs which cover every appendage that comprises the nose, some of which are used for initial prey detection, while others are for identification. Owing to the number of Eimer’s organs, their tiny size, and the way that the sensory cells respond to patterns of stimulation across parts of each individual Eimer’s organ, the mole obtains exquisite detail on texture, almost to a microscopic level. The chapter also discusses the highly refined tactile sense of spiders, looking at how they rely on vibrations transmitted through the ground, the silk web strands, or the surface waves and air for prey detection and capture. Spiders are equipped with a variety of sensors to detect mechanical information, including fine hairs sensitive to wind movement and touch, and special organs called slit sensilla around the joints of legs that measure physical forces acting on the exoskeleton. Finally, the chapter studies the nature and function of integumentary sense organs or ISOs in both crocodiles and alligators. The heavily built bodies of crocodiles and alligators belie a high sensitivity, being able to detect the slightest changes in touch and pressure.

Perch time allocation and feeding efficiency of flycatching Rhinolophus formosae: an optimal foraging behavior?

Background Flycatching bats are species-rare and comprise predominantly horseshoe bats (Rhinolophidae). Their hang-and-wait foraging mode and long constant-frequency echolocation calls offer advantages in energetics and prey detection, and may enable them apt to foraging optimally, yet not much is known about the foraging behavior of flycatching bats. Thus we assessed the perch use and foraging performance in the field by one of the largest horseshoe bats, Rhinolophus formosae, and offered insights on their perch time allocation. Results The perching-foraging behaviors of the bats did not differ significantly between forest settings, but the residence and giving-up time, mean attack, and attack rate were higher in the late spring-early summer, whereas the mean capture, capture rate, and attack efficiency were lower in the late summer when volant juveniles joined the nocturnal activity. The bats maintained flycatching and exhibited largely similar attack rates through the night with peak residence time around the midnight, but the capture rate and attack efficiency both reduced toward midnight and then increased toward the hours right before dawn. The attack rate was negatively correlated to the number of perches used and perch switch; by contrast, the capture rate was positively correlated with both factors. The total residence time at a site increased but mean residence time per perch decreased as the number of perches used and perch-switch increased. The giving-up time was inversely correlated to the attack rate and attack efficiency, and decreased with an increasing capture rate. Conclusions The bats increased perch switch at lower attack rates in early spring, but switched less frequently in late spring and prime summer months when insect abundance is higher. By scanning through a broad angular range for prey detection, and switching more frequently among perches, R. formosae foraged with an increased capture rate, and were able to remain at the site longer by slightly reducing their mean residence time per perch. Our results concur with the predictions of optimal foraging theory for patch selection and offer implications for further exploration of the foraging behavior of flycatching horseshoe bats.