Effect of Dairy Matrix on the Postprandial Blood Metabolome

This study investigated the postprandial plasma metabolome following consumption of four dairy matrices different in texture and structure: cheddar cheese (Cheese), homogenized cheddar cheese (Hom. Cheese), and micellar casein isolate (MCI) with cream (MCI Drink) or a MCI Gel. An acute, randomized, crossover trial in male participants (n = 25) with four test days was conducted. Blood samples were collected during an 8-h postprandial period after consumption of a meal similar in micro- and macronutrients containing one of the four dairy matrices, and the metabolome was analyzed using nuclear magnetic resonance (NMR) spectroscopy. A liquid dairy matrix (MCI Drink) resulted in a faster absorption of amino acids compared to products, representing either a semi-solid (MCI Gel and Hom. Cheese) or solid (Cheese) dairy matrix. For the MCI Gel, plasma concentration of acetic acid and formic acid increased approximately 2 h following consumption, while 3-hydroxybyturate and acetoacetic acid increased approximately 6 h after consumption. The structure and texture of the dairy matrix affected the postprandial absorption of amino acids, as revealed by the plasma metabolome. Our study furthermore pointed at endogenous effects associated with consumption of dairy products containing glucono-δ-lactone.

The influence of feed additives used to correct metabolic processes in the rumen cows to biochemical indicators of blood from after calving

Purpose: estimation of the possibility of using a mixture of feed additive "BioGrom SC" and the food antibiotic "Monenzin" for normalizing metabolic processes in highly productive cows in the transit period.Materials and methods. Studies were conducted because of the tribal plant of the Leningrad region. Two groups of animals were selected on the principle of analogs (experimental and control) 20 goals in each. The experimental group of cows 21 days before the calving and for 14 days after the calves were given a mixture of feed additive "BioGrom SC" and the food antibiotic "Monenzin". For the cottage additives, calcium carbonate (chalk) was used as a filler in the amount of 250 g with the addition of 10-15 g. Supplements "BioGrom SC" by 1 head. The mixture was prepared at the rate of 20 goals and a food antibiotic "Monenzin" was added to it at the rate of 100 mg per 1 kg of a mixture of feed additive with filler. The resulting mixture was fed by animals of the experimental group. All animal experimental and control groups take blood for 21 days before the hotel. The total protein, beta hydroxy acid, acetoacetic acid, alkaline blood reserve, calcium-phosphoric ratio, glucose is determined. At the end of the transit period on the 21st day of lactation, the blood of animal control and experimental groups was repeatedly investigated.Results. In the cows of the experimental group, in comparison with the cows of the control group at the end of the transit period, a decrease in such biochemical indicators was observed as beta hydroxy acid (a decrease to 0.84 ± 0.03 mmol / l), acetoacetic acid (decrease to 0.4 ± 0, 04 mmol / l). The animals of the experimental group decreased the content of the general protein (77.0 ± 0.08 g / l). Also, the data of the backup alkalinity of blood (acidose indicator) are within reference values. Calcium phosphorus ratio was also within the normal range.Conclusion. Introduction to the diet of a mixture of feed additive "BioGrom SC" and food antibiotic "Monenzin" normalize some biochemical blood indicators in cows in the transit period. At the same time, without correction of metabolic processes, cows are observed ketosis and acidosis, which can lead to premature disposal of animals from herd and as a result of economic losses. Thus, the use of the mixture is shown for the prevention and treatment of acidosis of the scar, metabolic acidosis, ketosis in cattle of the dairy direction of productivity.

Using Metabolomics to Differentiate Player Positions in Elite Male Basketball Games: A Pilot Study

Purpose: The current study compared metabolic profiles and movement patterns between different player positions and explored relationships between indicators of internal and external loads during elite male basketball games.Methods: Five main players from 14 basketball teams (n = 70) were selected as subjects and defined as backcourt (positions 1–3) or frontcourt (positions 4–5) players. Video-based time motion analysis (VBTMA) was performed based on players’ individual maximal speeds. Movements were classified into high and low intensity running with and without ball, high and low intensity shuffling, static effort and jumps. Saliva samples were collected before and after 40-min basketball games with metabolomics data being analyzed by multivariate statistics. Independent t-tests were used to compare VBTMA.Results: Frequency, duration, and distance of high and low intensity running and -shuffling were higher in backcourt players, whereas static effort duration and frequency as well as jump frequency were higher in frontcourt players (all p ≤ 0.05). The levels of taurine, succinic acid, citric acid, pyruvate, glycerol, acetoacetic acid, acetone, and hypoxanthine were higher in backcourt players, while lactate, alanine, 3-methylhistidine were higher and methionine was lower in frontcourt players (all p < 0.05). High intensity running with ball was significantly associated by acetylecholine, hopoxanthine, histidine, lactic acid and leucine in backcourt players (p < 0.05).Conclusion: We demonstrate different metabolic profiles of backcourt and frontcourt players during elite male basketball games; while aerobic metabolic changes are more present in backcourt players, frontcourt players showed lager changes in anaerobic metabolic pathways due to more static movements.

A RARE CASE MORTALITY OF DIABETIC KETOACIDOSIS- CEREBRAL EDEMA WITH TONSILLAR HERNIATION- A CONTRADICTORY OPINION.IS SODIUM BICARBONATE A REAL CULPRIT?

Diabetic ketoacidosis (DKA) is the most common complication seen in uncontrolled diabetes mellitus. DKA is most commonly seen with patients of type 1 diabetes. Depletion of Insulin leads to high blood sugars which in turn leads osmotic diuresis, production of ketone bodies i.e, βhydroxybutyric acid and acetoacetic acid, dysregulation of sodium hydrogen exchange mechanism[2]. As a consequence to the above stated mechanisms, cerebral edema has been documented as a fatal complication in DKA. Mortality documented due to cerebral edema is 21-25%[4].

Polycythemia Vera and Essential Thrombocythemia Patients Exhibit Unique Serum Metabolic Profiles Compared to Healthy Individuals and Secondary Thrombocytosis Patients

Most common myeloproliferative neoplasms (MPNs) include polycythemia vera (PV) and essential thrombocythemia (ET). Accurate diagnosis of these disorders remains a clinical challenge due to the lack of specific clinical or molecular features in some patients enabling their discrimination. Metabolomics has been shown to be a powerful tool for the discrimination between different hematological diseases through the analysis of patients’ serum metabolic profiles. In this pilot study, the potential of NMR-based metabolomics to characterize the serum metabolic profile of MPNs patients (PV, ET), as well as its comparison with the metabolic profile of healthy controls (HC) and secondary thrombocytosis (ST) patients, was assessed. The metabolic profile of PV and ET patients, compared with HC, exhibited higher levels of lysine and decreased levels of acetoacetic acid, glutamate, polyunsaturated fatty acids (PUFAs), scyllo-inositol and 3-hydroxyisobutyrate. Furthermore, ET patients, compared with HC and ST patients, were characterized by decreased levels of formate, N-acetyl signals from glycoproteins (NAC) and phenylalanine, while the serum profile of PV patients, compared with HC, showed increased concentrations of lactate, isoleucine, creatine and glucose, as well as lower levels of choline-containing metabolites. The overall analysis revealed significant metabolic alterations mainly associated with energy metabolism, the TCA cycle, along with amino acid and lipid metabolism. These results underscore the potential of metabolomics for identifying metabolic alterations in the serum of MPNs patients that could contribute to improving the clinical management of these diseases.

The association of low serum salivary and pancreatic amylases with the increased use of lipids as an energy source in non-obese healthy women

OBJECTIVE: It is unknown whether low serum levels of salivary and pancreatic amylases are associated with the high combustion of carbohydrates or lipids for energy. Elevated blood ketones and a low respiratory quotient (RQ) can reflect the preferential combustion of lipids relative to carbohydrates. Therefore, using the data from our previous study, we investigated if low levels of serum amylases were associated with a high serum ketone level and low RQ in 60 healthy non-obese young women aged 20–39 years old.RESULTS: Serum ketones [3-hydroxybutyric acid (3-HBA) and acetoacetic acid (AA)] were inversely correlated with RQs, but not body mass index (BMI) or glycated haemoglobin (HbA1c) levels. Logistic regression analysis showed that high levels of serum ketones (3-HBA ≥ 24 μmol/L and AA ≥ 17 μmol/L) and a low RQ (< 0.766) were significantly associated with low serum salivary (< 60 U/L) and pancreatic (< 29 U/L) amylase levels, respectively. These associations were not altered by further adjustments for age, BMI, HbA1c, and estimated glomerular filtration rate. These results confirm the high combustion of lipids for energy in individuals with low serum amylase levels, suggesting a close relationship between circulating amylases and internal energy production.

The association of low serum salivary and pancreatic amylases with the increased use of lipids as an energy source in non-obese healthy women

OBJECTIVE It is unknown whether serum levels of salivary and pancreatic amylases are associated with the high combustion of carbohydrates or lipids for energy. Elevated blood ketones and a low respiratory quotient (RQ) can reflect the preferential combustion of lipids relative to carbohydrates. Therefore, we investigated if low levels of serum salivary and pancreatic amylases were associated with a high serum ketone level and low RQ in healthy people. RESULTS Using the data from our previous study, we reanalysed clinical parameters, including serum salivary and pancreatic amylases, serum 3-hydroxybutyric acid (3-HBA) and acetoacetic acid (AA), and RQ in 60 healthy non-obese young women aged 20–39 years old. Serum ketones were inversely correlated with RQs, but not body mass index (BMI) or glycated haemoglobin (HbA1c) levels. Logistic regression analysis showed that high levels of serum ketones and a low RQ were significantly associated with low serum salivary (<60 U/L) and pancreatic (<29 U/L) amylase levels. These associations were not altered by further adjustments for age, BMI, HbA1c, and estimated glomerular filtration rate. These results confirm the high combustion of lipids for energy in individuals with low serum amylase levels, suggesting a close relationship between circulating amylases and internal energy production.

Maleic, glycolic and acetoacetic acids-leaching for recovery of valuable metals from spent lithium-ion batteries: leaching parameters, thermodynamics and kinetics

Environmentally friendly acid-leaching processes with three organic acids (maleic, glycolic and acetoacetic) were developed to recover valuable metals from the cathodic material of spent lithium-ion batteries (LiCoO 2 ). The leaching efficiencies of Li and Co by the maleic acid were 99.58% and 98.77%, respectively. The leaching efficiencies of Li and Co by the glycolic acid were 98.54% and 97.83%, while those by the acetoacetic acid were 98.62% and 97.99%, respectively. The optimal acid concentration for the maleic acid-, glycolic acid- and acetoacetic acid-leaching processes were 1, 2 and 1.5 mol l –1 , respectively, while their optimal H 2 O 2 concentrations were 1.5, 2 and 1.5 vol%, respectively. The optimal solid/liquid ratio, temperature and reaction time for the leaching process of the three organic acids was the same (10 g l −1 , 70°C, 60 min). The thermodynamic formation energy of the leaching products and the Gibbs free energy of the leaching reactions were calculated, and the kinetic study showed that the leaching processes fit well with the shrinking-core model. Based on the comparison in the leaching parameters, the efficacy and availability of the three acids is as follows: maleic acid > acetoacetic acid > glycolic acid.