First record of ranavirus (Ranavirus sp.) in Siberia, Russia

Ranaviruses are a group of double-strand DNA viruses that infect fish, amphibians and reptiles. These viruses are responsible for mass fish and amphibian mortality events worldwide, both in the wild and at the fish and amphibian farms. The number of detected epizootics has grown significantly in recent years. In Eastern Europe and Northern Asia, including Russia, very few ranavirosis monitoring studies have been conducted, in contrast with Western Europe and America. In the present work, we used a qPCR assay to survey for the first time the amphibian populations of West Siberia (Russia) for the presence of ranaviruses. In total, we studied 252 tissue samples from six amphibian species, collected across West Siberia from the south to the Arctic regions. We report a single infected sample: a common toad (Bufo bufo) captured near Tyumen city. The phylogenetic analysis showed that the detected virus strain belongs to the CMTV lineage. This is only the second observation of Ranavirus in Russia.

Global distribution of mammal herbivore biomass reveals megafauna extinction patterns

Terrestrial mammalian herbivores strongly shape ecosystems and influence Earth system processes. Herbivorous mammals can alter vegetation structure, accelerate nutrient distribution, and modify carbon cycling. The Late Pleistocene megafauna extinctions triggered significant changes in ecosystems and climate, and current extinctions are having similarly pervasive consequences. A lack of global dynamic models of mammal populations limits our understanding of the ecological role of wild mammals and the consequences of their past and future extinctions. Here we present a global model of herbivore mammal populations defined by their ecological role based on a classification of all extant herbivores (n = 2599) in 24 functional groups. The eco-physiological model predicts present-day mammal biomass in natural conditions. Biomass hotspots occur in areas today dominated by humans, which account for 30% of biomass loss and limit future rewilding potentials. Large herbivore (body mass > 5 kg) biomass is higher in hot and wet areas with high evapotranspiration. Conversely, small herbivore biomass is more evenly distributed, particularly in colder climates. Thus, energy-water dependency is higher in large herbivores than smaller ones. Negative deviations from the biomass and water-energy relationship unveil past extinction patterns. Late Pleistocene extinctions may have triggered a collapse of biomass in Australia and South America and heavy losses in North America and northern Asia. The herbivore biomass estimates provide a quantitative benchmark for conservation and management actions. The herbivore model and the functional classification create new opportunities to integrate mammals into Earth system science.

Variations in Summer Extreme High-Temperature Events over Northern Asia and the Possible Mechanisms

In this study, interannual and interdecadal variations in the extreme high-temperature event (EHE) frequency over Northern Asia (NA) and the associated possible mechanisms are explored. On an interannual timescale, the first two empirical orthogonal function modes of the NA EHE frequency exhibit meridional dipole pattern (EOF1) and diagonal tripolar pattern (EOF2), respectively. The higher NA EHE frequency is related to anomalous local highs, reduced middle-low clouds and more solar radiation. The warmer ground further heats the overlying atmosphere through longwave radiation and sensible heat. The warm temperature advections in lower troposphere and the drier soil conditions also favor higher EHE frequency. Further analysis reveals that the EOF1 mode is related to the Polar-Eurasian teleconnection pattern (POL), while the EOF2 mode is associated with North Atlantic Oscillation (NAO) and Pacific-Japan/East Asia-Pacific pattern (PJ/EAP). The fitted EHE frequency based on the atmospheric factors (POL, NAO and PJ/EAP) can explain the interannual variation in the regionally averaged EHE frequency by 33.8%. Furthermore, three anomalous sea surface temperature (SST) patterns over the North Atlantic-Mediterranean Sea region and around the Maritime Continent are associated with the two EHE modes by intensifying the pronounced atmospheric teleconnections. Analysis on the simulation of five models in the Atmospheric Model Intercomparison Project experiment further confirms the impact of the pronounced SST patterns on the POL, NAO and PJ/EAP. In addition, NA EHE frequency experienced a significant interdecadal increase around the mid-1990s, which could be associated with the phase shift of the Atlantic Multidecadal Oscillation and long-term global warming trend.

The global hepatitis delta virus (HDV) epidemic: what gaps to address in order to mount a public health response?

Background Co-infection between hepatitis B virus (HBV) and hepatitis delta virus (HDV) causes the severest chronic hepatitis and is associated with a high risk of cirrhosis and hepatocellular carcinoma (HCC). The Global Health Sector Strategy on Viral Hepatitis called for the elimination of hepatitis (− 65% mortality and − 90% incidence) by 2030. Our aims were to summarize key points of knowledge and to identify the gaps that need to be addressed to mount a public health response to HDV. Methods We performed a current literature review in terms of epidemiology by WHO regions, genotypes distribution and their pathogenicity, factors associated with HDV infection, mortality due to HDV infection, testing strategies and treatment. Results Prevalence of infection and genotypes are heterogeneous distributed, with highest prevalence in foci around the Mediterranean, in the Middle East, and in Central, Northern Asia and Eastern Asia. Persons who inject drugs (PWID) and migrants from highly endemic areas are highly affected. While antibody detection tests are available, HDV RNA tests of current infection are not standardized nor widely available. The few therapeutic options, including lofartinib, are not widely available; however several new and promising agents have entered clinical trials. Conclusion HDV infection is an poorly known cause of chronic liver disease. To mount a public health response, we need a better description of the HDV epidemic, standardized testing strategies and better treatment options.

Distribution of alpine endemic plants of northern Asia: a dataset

We describe a dataset providing information on the geographic distribution of northern Asian endemic alpine plants. It was obtained by digitising maps from the atlas “Endemic alpine plants of Northern Asia”. Northern Asia includes numerous mountain ranges which may have served as refugia during the Pleistocene ice ages, but there have been no studies that analysed this question. We suggest that this dataset can be applied for better understanding of the alpine endemism in northern Asia. The dataset includes 13709 species distribution records, representing 211 species from 31 families and 106 genera. Each record provides data regarding the distribution of an individual species. These data provide a foundation for studying northern Asia's endemic alpine species and conducting research on the factors concerning their distribution.

Neoehrlichia mikurensis Causing Thrombosis and Relapsing Fever in a Lymphoma Patient Receiving Rituximab

Neoehrlichia (N.) mikurensis, an intracellular tick-borne bacterium not detected by routine blood culture, is prevalent in ticks in Scandinavia, Central Europe and Northern Asia, and may cause long-standing fever, nightly sweats, migrating pain, skin rashes and thromboembolism, especially in patients treated with rituximab. The multiple symptoms may raise suspicion of both infection, inflammation and malignancy, and lead in most cases to extensive medical investigations across many medical specialist areas and a delay of diagnosis. We describe a complex, albeit typical, case of neoehrlichiosis in a middle-aged splenectomised male patient with a malignant lymphoma, receiving treatment with rituximab. The multifaceted clinical picture associated with this tick-borne disease is addressed, and longitudinal clinical and laboratory data, as well as imaging, are provided. Longstanding relapsing fever in combination with thrombosis in superficial and deep veins in an immunocompromised patient living in a tick-endemic region should raise the suspicion of the emerging tick-borne disease neoehrlichiosis. Given the varied clinical presentation and the risk of delay in diagnosis and treatment, we believe it is important to raise clinicians’ awareness of this emerging infection, which is successfully treated with doxycycline.

Foreign Direct Investment (FDI), Investment in Construction and Poverty in Economic Crises (Denmark, Italy, Germany, Romania, China, India and Russia)

This study aims to examine the impact of foreign direct investment (FDI), investment in construction and poverty in various countries. The Russian Federation invests heavily in construction and it is located both in Europe and Asia. Russia is usually described as a European country (while 70% of its territory is in Northern Asia, 80% of the population resides in Europe). That is why in this document both developed and emerging countries are considered; the former are represented by the EU members of different economic levels and the latter by BRICS countries. We looked at economically different countries to determine the best differentiated data in order to answer the question: “Why does a high level of poverty persist in Russia if Russian officials have repeatedly reaffirmed their commitment to the implementation of the Sustainable Development Goals (SDGs) by investing heavily in construction and attracting FDI?”. For the estimation, we used an autoregressive distributed lag (ARDL), considering cointegration and heteroscedasticity, in which the current values of the series depend both on the past values of this series and on the current and past values of other time series. Having received statistical data, we were able to compare the economic development of countries with some economic growth theories. 4–5% FDI share of the GDP helps to contain the negative impact of financial crises. Investment in construction supports the economies of countries in the long term and maintains or reduces the poverty level by increasing the assets of the population. Empirical data also helped us to evaluate the economic growth patterns and poverty in these seven countries. China and the Russian Federation will find themselves at different “poles”. China uses several theories and models simultaneously for economic development and poverty reduction and the Russian Federation does not keep to an established theory or a model of economic growth.

The role of IFITM proteins in tick-borne encephalitis virus infection

Tick-borne encephalitis virus (TBEV), of the genus Flavivirus, is a causative agent of severe encephalitis in endemic regions of northern Asia and central and northern Europe. Interferon induced transmembrane proteins (IFITMs) are restriction factors that inhibit the replication cycles of numerous viruses, including flaviviruses such as the West Nile virus, dengue virus, and Zika virus. Here, we demonstrate the role of IFITM1, IFITM2, and IFITM3 in the inhibition of TBEV infection and in protection against virus-induced cell death. We show the most significant role being that of IFITM3, including the dissection of its functional motifs by mutagenesis. Furthermore, through the use of CRISPR–Cas9-generated IFITM1/3-knockout monoclonal cell lines, we confirm the role and additive action of endogenous IFITMs in TBEV suppression. However, the results of co-culture assays suggest that TBEV might partially escape IFN- and IFITM-mediated suppression during high-density co-culture infection when the virus enters naïve cells directly from infected donor cells. Thus, cell-to-cell spread may constitute a strategy for virus escape from innate host defenses. Importance: TBEV infection may result in encephalitis, chronic illness or death. TBEV is endemic in northern Asia and Europe; however, due to climate change, new endemic centers arise. Although effective TBEV vaccines have been approved, vaccination coverage is low, and, due to the lack of specific therapeutics, infected individuals depend on their immune responses to control the infection. The IFITM proteins are components of the innate antiviral defenses that suppress cell entry of many viral pathogens. However, no studies regarding the role of IFITM proteins in the TBEV infection have been published so far. Understanding of antiviral innate immune responses is crucial for future development of antiviral strategies. Here, we show the important role of IFITM proteins in the inhibition of TBEV infection and virus-mediated cell death. However, our data suggest that TBEV cell-to-cell spread may be less prone to both IFN- and IFITM-mediated suppression, potentially facilitating escape from IFITM-mediated immunity.