Biochemical Characterization of Two Rhamnogalacturonan Lyases From Bacteroides ovatus ATCC 8483 With Preference for RG-I Substrates

Rhamnogalacturonan lyase (RGL) cleaves backbone α-1,4 glycosidic bonds between L-rhamnose and D-galacturonic acid residues in type I rhamnogalacturonan (RG-I) by β-elimination to generate RG oligosaccharides with various degrees of polymerization. Here, we cloned, expressed, purified and biochemically characterized two RGLs (Bo3128 and Bo4416) in the PL11 family from Bacteroides ovatus ATCC 8483. Bo3128 and Bo4416 displayed maximal activity at pH 9.5 and pH 6.5, respectively. Whereas the activity of Bo3128 could be increased 1.5 fold in the presence of 5 mM Ca2+, Bo4416 required divalent metal ions to show any enzymatic activity. Both of RGLs showed a substrate preference for RG-I compared to other pectin domains. Bo4416 and Bo3128 primarily yielded unsaturated RG oligosaccharides, with Bo3128 also producing them with short side chains, with yields of 32.4 and 62.4%, respectively. Characterization of both RGLs contribute to the preparation of rhamnogalacturonan oligosaccharides, as well as for the analysis of the fine structure of RG-I pectins.

Deformation Characteristic of a Supported Deep Excavation System: A Case Study in Red Sandstone Stratum

A complete case record of a deep foundation pit with pile-anchor retaining structure excavated in red sandstone stratum is presented in this study. The horizontal displacement of pile top, the horizontal displacement at various depths, the axial force of anchor cable, and ground settlement during construction are measured. A three-dimensional numerical model is established to analyze the additional stress and deformation induced by the excavation and the accuracy of the FEM model is verified by comparing with field measured results. Both the measured and numerical simulation results show that the deformation of the pile-anchor supported deep excavation is significantly affected by the spatial effect. The results show that the deformation in the middle of the foundation pit is greater than the pit angle and that the deformation of the long side is greater than that of the short side and gradually decreases from the middle to the pit angle. The deformation and stress in the middle of the long side of the foundation pit are the largest, which is the most unfavorable part. With the increase of vertical excavation depth, the spatial effects tend to increase, and the influence scope of spatial effects is about five times the vertical excavation depth in the red sandstone stratum. The ground settlement outside the pit is mainly distributed in a groove shape, and the maximum settlement occurs about 8.5 m away from the pit edge. Finally, parametric studies of reinforcement parameters indicated that 1.5–2.0 times the initial elastic modulus and cohesive force of soil should be used for reinforcement. It is recommended that the ranges for pile diameter, pile spacing, anchor cable prestressing and inclination angle should be selected as 0.8–1.2 m, 1.4–2.0 m, 100–150 kN, and 10°–20°, respectively.

Superior Proton Exchange Membrane Fuel Cell (PEMFC) Performance Using Short-Side-Chain Perfluorosulfonic Acid (PFSA) Membrane and Ionomer

Optimization of the ionomer materials in catalyst layers (CLs) which sometimes is overlooked has been equally crucial as selection of the membranes in membrane electrode assembly (MEA) for achieving a superior performance in proton exchange membrane fuel cells (PEMFCs). Four combinations of the MEAs composed of short-side-chain (SSC) and long-side-chain (LSC) perfluorosulfonic acid (PFSA) polymers as membrane and ionomer materials have been prepared and tested under various temperatures and humidity conditions, aiming to investigate the effects of different side chain polymer in membranes and CLs on fuel cell performance. It is discovered that SSC PFSA polymer used as membrane and ionomer in CL yields better fuel cell performance than LSC PFSA polymer, especially at high temperature and low RH conditions. The MEA with the SSC PFSA employed both as a membrane and as an ionomer in cathode CL demonstrates the best cell performance amongst the investigated MEAs. Furthermore, various electrochemical diagnoses have been applied to fundamentally understand the contributions of the different resistances to the overall cell performance. It is illustrated that the charge transfer resistance (Rct) made the greatest contribution to the overall cell resistance and then membrane resistance (Rm), implying that the use of the advanced ionomer in CL could lead to more noticeable improvement in cell performance than only the substitution as the membrane.

Improved Thermo-Mechanical Properties and Reduced Hydrogen Permeation of Short Side-Chain Perfluorosulfonic Acid Membranes Doped with Ti3C2Tx

Benefiting from its large specific surface with functional -OH/-F groups, Ti3C2Tx, a typical two-dimensional (2D) material in the recently developed MXene family, was synthesized and used as a filler to improve the properties of the short side-chain (SSC) perfluorosulfonic acid (PFSA) proton exchange membrane. It is found that the proton conductivity is enhanced by 15% while the hydrogen permeation is reduced by 45% after the addition of 1.5 wt% Ti3C2Tx filler into the SSC PFSA membrane. The improved proton conductivity of the composite membrane could be associated with the improved proton transport environment in the presence of the hydrophilic functional groups (such as -OH) of the Ti3C2Tx filler. The significantly reduced hydrogen permeation could be attributed to the incorporation of the impermeable Ti3C2Tx 2D fillers and the decreased hydrophilic ionic domain spacing examined by the small angle X-ray scattering (SAXS) for the composite membrane. Furthermore, improved thermo-mechanical properties of the SSC/Ti3C2Tx composite membrane were measured by dynamic mechanical analyzer (DMA) and tensile strength testing. The demonstrated higher proton conductivity, lower hydrogen permeation, and improved thermo-mechanical stability indicate that the SSC/Ti3C2Tx composite membranes could be a potential membrane material for PEM fuel cells operating above the water boiling temperature.

Novel piece of the puzzle: ALI1 is required for oxo-C14-HSL priming in Arabidopsis

Quorum sensing (QS) molecules mediate communication between bacterial cells. N-acyl homoserine lactones (AHL) are one of the best-studied groups of QS molecules. In addition to bacterial communication, AHL are involved in interactions with eukaryotes. Short side-chain AHL are readily taken up by plants. They induce root elongation and growth promotion. Hydrophobic long side-chain AHL are usually not transported over long distances although, they may prime plants for enhanced resistance. Unfortunately, studies elucidating the plant factors required for response to AHL are sparse. Here, we provide evidence of a plant protein, namely the AHL-priming protein 1 (ALI1), indispensable for enhanced resistance response induced by the N-3-oxotetradecanoyl-homoserine lactone (oxo-C14-HSL). Comparing Col-0 and the ali1 mutant, we revealed loss of AHL-priming in ali1. This phenomenon is reverted with the reintroduction of ALI1 into ali1. Additional transcriptome analysis revealed that ali1 is less sensitive to oxo-C14-HSL treatment compared to the wild-type. Our results suggest, therefore, that ALI1 is required for oxo-C14-HSL-dependent priming for enhanced resistance in Arabidopsis.

Research on Children’s Customized Furniture Design Based on Group Technology

Recently, solid wood furniture has become the new direction for the development of Chinese furniture industry. In order to realize solid wood customization, the standardization of solid wood parts is a problem for high priority. In this study, a standardized experimental study on children’s solid wood furniture parts was carried out by using group technology. Twenty pieces of children’s solid wood furniture were selected, including 1084 parts in total with 705 solid wood parts. Then, three key structural features of the parts were analyzed based on the processing similarity principle, including the center line of the long side direction of the parts, the shape of the outside surface of the long side direction and the shape of the outside surface of the short side direction. Moreover, these parts were classified according to the principle of process similarity. Accordingly, a children’s solid wood furniture parts family was established, and the distribution of parts in the family was analyzed. In detail, the parts with the dimensional difference within 2 mm were combined, the dimensional value was mainly based on the value of most parts before the combination and part specifications were optimized on the basis of the original specifications or its 1/2 method. The results show that the category of thickness specification reduced by 20, and the number of parts included in one thickness specification increased by 2.2 times on average. Moreover, the category of width specification reduced by 12, and the number of parts included in one width specification increased by 1.47 times on average. This not only greatly improves the degree of standardization of solid wood parts but also provides theoretical and practical basis for the digital design of mass customized children’s solid wood furniture.

Study of interface gas-liquid flow characteristics in a rectangular microchannel for wavy-annular flow

The aim of this work is an experimental study of a gas-liquid flow in a rectangular slit microchannel with a cross-section of 200 × 2045 μm. Ethanol/water (95/5) mixture and nitrogen are used as working liquid and gas, accordingly. The external T-mixer is used for obtaining of wavy-annular flow pattern. The experimental data on interfacial waves and their characteristics in the meniscus area on the short side of the microchannel are obtained using high-speed visualization for a wide range of gas and liquid superficial velocities. Images are processed using the Python libraries to define the average liquid layer thickness and maximum amplitude of waves. An increase of gas superficial velocity causes decreasing in the average liquid layer thickness and maximal amplitude of the liquid layer thickness. The waves on the liquid layer surface (maximal amplitude) can be three times larger than the average liquid layer thickness for presented liquid and gas velocities. With increasing gas superficial velocities more liquid displace from the meniscus area to the liquid film on the wide side of the microchannel.

Experimental Study on Single Corner Cold Bending Mechanical Response of Laminated of PVB Interlayer Tempered Glass Panes and the Coupling Effect with Load

The cold bending method is a type of curved glass curtain wall construction method that has been used in practical engineering for a short time. It has the advantages of simple operation, high efficiency and low cost. However, the mechanical response and properties of glass panes caused by cold bending have not been solved effectively. To study the mechanical response and the properties of cold formed laminated tempered glass panes after applying with a wind load, cold bending and load tests of 9 laminated tempered glass panes were conducted by the orthogonal experimental design method. The effects of cold bending curvature, glass pane thickness and interlayer thickness were considered. In this paper, the response law of cold bending stress to the curvature and the relationship among the influencing factors were analyzed. The variation process of stress, the deflection of cold-formed glass panes under uniform load and the characteristics affected by cold-formed stress and deformation were studied. The results show that the cold bending stress is distributed in a saddle shape, and the curvature has the greatest influence on the cold bending stress, followed by the thickness of the glass panes. The influence of the interlayer thickness is small. The maximum stress appears near the corner of the short side direction adjacent to the cold bending corner. The cold bending stress increases linearly with increasing cold bending curvature. The cold bending stress and deformation have little effect on the change process of the later stage load effect.

Thermoresponsive Molecular Brushes with a Rigid-Chain Aromatic Polyester Backbone and Poly-2-alkyl-2-oxazoline Side Chains

A new polycondensation aromatic rigid-chain polyester macroinitiator was synthesized and used to graft linear poly-2-ethyl-2-oxazoline as well as poly-2-isopropyl-2-oxazoline by cationic polymerization. The prepared copolymers and the macroinitiator were characterized by NMR, GPC, AFM, turbidimetry, static, and dynamic light scattering. The molar masses of the polyester main chain and the grafted copolymers with poly-2-ethyl-2-oxazoline and poly-2-isopropyl-2-oxazoline side chains were 26,500, 208,000, and 67,900, respectively. The molar masses of the side chains of poly-2-ethyl-2-oxazoline and poly-2-isopropyl-2-oxazoline and their grafting densities were 7400 and 3400 and 0.53 and 0.27, respectively. In chloroform, the copolymers conformation can be considered as a cylinder wormlike chain, the diameter of which depends on the side chain length. In water at low temperatures, the macromolecules of the poly-2-ethyl-2-oxazoline copolymer assume a wormlike conformation because their backbones are well shielded by side chains, whereas the copolymer with short side chains and low grafting density strongly aggregates, which was visualized by AFM. The phase separation temperatures of the copolymers were lower than those of linear analogs of the side chains and decreased with the concentration for both samples. The LCST were estimated to be around 45 °C for the poly-2-ethyl-2-oxazoline graft copolymer, and below 20 °C for the poly-2-isopropyl-2-oxazoline graft copolymer.