scholarly journals Functionality of Silk Cocoon (Bombyx mori L.) Sericin Extracts Obtained through High-Temperature Hydrothermal Method

Materials ◽  
2021 ◽  
Vol 14 (18) ◽  
pp. 5314
Author(s):  
Wei-Hsun Wang ◽  
Wen-Shin Lin ◽  
Chia-Hung Shih ◽  
Cheng-You Chen ◽  
Siao-Hong Kuo ◽  
...  
Keyword(s):  
Vitamin C ◽  
Hydrothermal Method ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Dry Weight ◽  
Total Phenol Content ◽  
Tyrosinase Inhibition ◽  
Flavonoid Content ◽  
Silk Cocoon ◽  
Radical Scavenging Ability

Sericin, a textile waste, can be used for antioxidant and skin-whitening purposes. The hydrothermal method of extracting sericin is more eco-friendly than are chemical and enzymatic methods. In this study, silk cocoons were cut into pieces and then subjected to hydrothermal extraction at three temperatures (160, 200, and 220 °C) to obtain sericin extracts (Sericin160, Sericin200, and Sericin220, respectively). Antioxidant activity and tyrosinase inhibition were measured to determine the extracts’ effectiveness. Sericin220 was the strongest antioxidant, with total phenol content, total flavonoid content, and ferric reducing power of 62.19 ± 0.04 mg gallic acid equivalents/g dry weight, 0.07 ± 0.01 mg quercetin equivalent/g dry weight, and 181.49 ± 0.024 mg vitamin C equivalent/g dry weight, respectively. The half-maximal inhibitory concentrations for DPPH and ABTS free-radical scavenging ability were 6.41 ± 0.05 and 0.79 ± 0.37 mg/mL, respectively. Sericin220 also exhibited the highest tyrosinase inhibition activity (70.82 ± 4.1 mg vitamin C equivalent/g), indicating its whitening potential.

scholarly journals Effects of Different Chenopodium formosanum Parts on Antioxidant Capacity and Optimal Extraction Analysis by Taguchi Method

Materials ◽  
2021 ◽  
Vol 14 (16) ◽  
pp. 4679
Author(s):  
Chin-Tung Wu ◽  
Wei-Hsun Wang ◽  
Wen-Shin Lin ◽  
Shiou-Yih Hu ◽  
Cheng-You Chen ◽  
...  
Keyword(s):  
Taguchi Method ◽  
Agricultural Waste ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Waste Reduction ◽  
Total Phenol Content ◽  
Native Plant ◽  
Flavonoid Content ◽  
Radical Scavenging Ability ◽  
Dpph Radical Scavenging

Chenopodium formosanum (CF), rich in nutrients and antioxidants, is a native plant in Taiwan. During the harvest, the seeds are collected, while the roots, stems, and leaves remain on the field as agricultural waste. In this study, di(phenyl)-(2,4,6-trinitrophenyl)iminoazanium (DPPH) radical scavenging ability and 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical scavenging ability experiments of seeds, leaves, stems, and roots were designed using the Taguchi method (TM) under three conditions: Ethanol concentration (0–100%), temperature (25–65 °C), and extraction time (30–150 min). The result demonstrates that seeds and leaves have higher radical scavenging ability than stems and roots. Many studies focused on CF seeds. Therefore, this study selected CF leaves and optimized DPPH, ABTS, total phenol content (TPC), total flavonoid content (TFC), and reducing power (RP) through TM, showing that the predicted value of the leaf is close to the actual value. The optimized results of CF leaves were DPPH 85.22%, ABTS 46.51%, TPC 116.54 µg GAE/mL, TFC 143.46 µg QE/mL, and RP 23.29 µg VCE (vitamin C equivalent)/mL. The DPPH and ABTS of CF leaves were second only to the results of CF seeds. It can be seen that CF leaves have the potential as a source of antioxidants and help in waste reduction.

Differential Antioxidant Composition and Potential of some commonly used Indian Spices

2017 ◽  
Vol 4 (03) ◽  
Author(s):  
SHARDA CHOUDHARY ◽  
ANDY PEREIRA ◽  
SUPRATIM BASU ◽  
A. K. VERMA
Keyword(s):  
Antioxidant Capacity ◽  
Curcuma Longa ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antioxidant Potential ◽  
Flavonoid Content ◽  
Food Preservatives ◽  
Nitric Oxide Radical ◽  
Radical Scavenging Ability ◽  
Phytochemical Components

Spices and herbs have been added to Indian foods not only as flavoring agents but also as natural supplements of medicines and food preservatives. The purpose of this study was to study the composition and antioxidant potential of some commonly used seed spices and derive a correlation between their phytochemical components and antioxidant capacity. Crushed seeds of cumin (Cuminumcyminum), coriander (Coriandrumsativum), fennel (Foeniculumvulgare), fenugreek (Trigonellafoenum-graecum), ajwain (Trachyspermumammi) and turmeric (Curcuma longa)were usedto prepare methanolic extract and iron(III) reduction, DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging, hydrogen peroxide , superoxide and nitric oxide radical scavenging, reducing power were assayed as an index of the antioxidant capacity along with phenolics and flavonoids were also estimated. In addition to turmeric, ajwain, fennel and coriander showed higher phenolic, flavonoid content, and antioxidant potential in comparison to fenugreek and cumin. The extracts of these spices are promising sources of alternative medicine with high free radical scavenging ability OF synthetic antioxidants and can also be used for therapeutic purposes as well.

scholarly journals Statistical Optimization of Flavonoid and Antioxidant Recovery from Macerated Chinese and Malaysian Lotus Root (Nelumbo nucifera) Using Response Surface Methodology

Molecules ◽  
2021 ◽  
Vol 26 (7) ◽  
pp. 2014
Author(s):  
Sze-Jack Tan ◽  
Chee-Keong Lee ◽  
Chee-Yuen Gan ◽  
Olusegun Abayomi Olalere
Keyword(s):  
Radical Scavenging ◽  
Reducing Power ◽  
Total Flavonoid ◽  
Quadratic Model ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Lotus Root ◽  
Reducing Power Assay ◽  
Experimental Values ◽  
Sample Ratio

In this study, the combination of parameters required for optimal extraction of anti-oxidative components from the Chinese lotus (CLR) and Malaysian lotus (MLR) roots were carefully investigated. Box–Behnken design was employed to optimize the pH (X1: 2–3), extraction time (X2: 0.5–1.5 h) and solvent-to-sample ratio (X3: 20–40 mL/g) to obtain a high flavonoid yield with high % DPPHsc free radical scavenging and Ferric-reducing power assay (FRAP). The analysis of variance clearly showed the significant contribution of quadratic model for all responses. The optimal conditions for both Chinese lotus (CLR) and Malaysian lotus (MLR) roots were obtained as: CLR: X1 = 2.5; X2 = 0.5 h; X3 = 40 mL/g; MLR: X1 = 2.4; X2 = 0.5 h; X3 = 40 mL/g. These optimum conditions gave (a) Total flavonoid content (TFC) of 0.599 mg PCE/g sample and 0.549 mg PCE/g sample, respectively; (b) % DPPHsc of 48.36% and 29.11%, respectively; (c) FRAP value of 2.07 mM FeSO4 and 1.89 mM FeSO4, respectively. A close agreement between predicted and experimental values was found. The result obtained succinctly revealed that the Chinese lotus exhibited higher antioxidant and total flavonoid content when compared with the Malaysia lotus root at optimum extraction condition.

scholarly journals Comparative Study on Phytochemical Profile and Antioxidant Activity of an Epiphyte, Viscum album L. (White Berry Mistletoe), Derived from Different Host Trees

Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1191
Author(s):  
Mahak Majeed ◽  
Tanveer Bilal Pirzadah ◽  
Manzoor Ahmad Mir ◽  
Khalid Rehman Hakeem ◽  
Hesham F. Alharby ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Pearson Correlation ◽  
Juglans Regia ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Viscum Album ◽  
Total Phenol Content ◽  
Leaf Extracts ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power

The study aimed at evaluating the antioxidant profile of a medicinal epiphyte Viscum album L. harvested from three tree species, namely, Populus ciliata L, Ulmus villosa L., and Juglans regia L. The crude extracts were obtained with ethanol, methanol, and water and were evaluated for the total phenol content (TPC), total flavonoid content (TFC), and antioxidant activities using total reducing power (TRP), ferric reducing antioxidant power (FRAP), 1, 1-diphenyl 1-2-picryl-hydrazyl (DPPH), superoxide radical scavenging (SOR), and hydroxyl radical scavenging (•OH) assays. Our results showed that crude leaf extracts of plants harvested from the host Juglans regia L. exhibited higher yields of phytochemical constituents and noticeable antioxidative properties. The ethanolic leaf samples reported the highest phenols (13.46 ± 0.87 mg/g), flavonoids (2.38 ± 0.04 mg/g), FRAP (500.63 ± 12.58 μM Fe II/g DW), and DPPH (87.26% ± 0.30 mg/mL). Moreover, the highest values for TRP (4.24 ± 0.26 μg/mL), SOR (89.79% ± 0.73 mg/mL), and OH (67.16% ± 1.15 mg/mL) were obtained from aqueous leaf extracts. Further, Pearson correlation was used for quantifying the relationship between TPC, TFC, and antioxidant (FRAP, DPPH, SOR, OH) activities in Viscum album L. compared to their hosts. It was revealed that the epiphyte showed variation with the type of host plant and extracting solvent.

scholarly journals Research antioxidant activity of chitooligosacchride by UV-VIS absorption spectrocopy

2020 ◽  
Vol 8 (1) ◽  
pp. 75-81
Author(s):  
Bui Van Hoai ◽  
Ngo Dai Nghiep ◽  
Dao An Quang ◽  
Nguyen Thi Nam Phuong
Keyword(s):  
Molecular Weight ◽  
Antioxidant Activity ◽  
Absorption Spectrum ◽  
Free Radical ◽  
Trichoderma Viride ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Degree Of Deacetylation ◽  
Radical Scavenging Ability

Chitosan with 80% degree of deacetylation was hydrolyzed by cellulase of Trichoderma viride to prepare chitooligosaccharides (COSs) by the fractionation of the COSs with ultrafiltration membrane. The antioxidant activities of the COSs were clarified in this study by reducing power and free radical scavenging ability assay by UV-VIS absorption spectrum. The results show that the COS 1 (10,000-5,000 Da), COS 2 (5,000-3,000 Da), COS 3 (3,000-1,000 Da) and COS 4 (less than1,000 Da) segments have antioxidant properties.The antioxidant activitives of the COSs increased with the increment of concentration, and they also depended on molecular weight.

scholarly journals Investigation of the Key Pharmacological Activities of Ficus racemosa and Analysis of Its Major Bioactive Polyphenols by HPLC-DAD

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Salma Akter Sumi ◽  
Md. Afjalus Siraj ◽  
Amir Hossain ◽  
Md. Sagir Mia ◽  
Seagufta Afrin ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Hydroxyl Radical ◽  
Cytotoxic Activity ◽  
Superoxide Radical ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Tannin Content ◽  
Phenolic Constituents ◽  
Ficus Racemosa ◽  
Radical Scavenging Ability

Objective. Oxidative stress leads to numerous physiological disorders including infectious diseases, inflammation, and cancer. The present study was carried out to investigate antioxidant, antibacterial, and cytotoxic activity of methanol crude extract of leaves and fruits of the Ficus racemosa (LCME and FCME, resp.) and to analyse its major bioactive polyphenols by HPLC-DAD. Methods. Antioxidant capacity of the extracts was evaluated by DPPH free radical scavenging, reducing power, total phenolic, total flavonoid, total tannin content assay, superoxide radical, hydroxyl radical, and hydrogen peroxide scavenging assay. Identification and quantification of bioactive polyphenols were done by HPLC-DAD method. Antibacterial activity was tested by “disc diffusion” method. Brine shrimp lethality assay was carried out to check the cytotoxic potential. Result. Both LCME and FCME showed DPPH scavenging ability and concentration dependent reducing power activity. They had phenolic content, flavonoid content, and tannin content. Both the extracts showed superoxide radical scavenging ability, hydroxyl radical scavenging ability, and hydrogen peroxide scavenging ability. HPLC analysis of LCME and FCME indicated the presence of significant amount of gallic acid along with other phenolic constituents. Conclusion. Significant amount of gallic acid along with other phenolic constituents might have played an important role in the observed antioxidant, antibacterial, and cytotoxic activity.

scholarly journals In VitroAntioxidant Properties of Three Varieties ofAllium sativumL. Extracts

2010 ◽  
Vol 7 (s1) ◽  
pp. S573-S579 ◽  
Author(s):  
R. T. Narendhirakannan ◽  
K. Rajeswari
Keyword(s):  
Free Radical ◽  
Allium Sativum ◽  
Preventive Intervention ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Garlic Bulb ◽  
Total Antioxidant ◽  
Radical Scavenging Ability ◽  
Dpph Radical Scavenging

Many herbs possess antioxidant ingredients that provide efficacy by additive or synergistic activities.Allium sativumL. is a strong astringent, used for the treatment of liver and spleen diseases, rheumatism and tumors. The antioxidant activities of different concentrations of ethanolic extracts of garlic bulb of three varieties were determined by the four assaysi.e. DPPH radical scavenging assay, reducing power ability, hydrogen peroxide scavenging assay and total antioxidant capacity. Due to its natural origin and potent free-radical scavenging ability,Allium sativumL. could be used as a potential preventive intervention for free radical-mediated diseases.

Antihemolytic and antioxidant activities of Allium paradoxum

2010 ◽  
Vol 5 (3) ◽  
pp. 338-345 ◽  
Author(s):  
Mohammad Ebrahimzadeh ◽  
Seyed Nabavi ◽  
Seyed Nabavi ◽  
Bahman Eslami
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Aerial Part ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Dependent Manner ◽  
Vitro Assay ◽  
Aerial Parts

AbstractAntioxidant activity of the aerial part and bulbs of Allium paradoxum was investigated by eight in vitro assay systems. Extracts showed good antioxidant activity. IC50 for 1,1-diphenyl-2-picryl hydrazyl radical-scavenging activity was 890.9±43.2 and 984.9±33.5 µg/ml for the aerial part and bulbs, respectively. The aerial parts have better reducing power than bulb extracts but not comparable with Vitamin C (P>0.001). Extracts showed weak Fe2+ chelating ability, the IC50 being 959±47 and 530±24 µg/ml for bulbs and aerial parts, respectively. Both tested extracts exhibited good hydrogen peroxide scavenging in a concentration dependent manner. They exhibited good antioxidant activity against the hemoglobin-induced linoleic acid system that was comparable with vitamin C (P>0.01). They showed good activity against cumene hydro peroxide induced hemolysis in RBCs. In addition, they possessed antihemolytic activity. The extract from aerial parts had significantly higher total phenol and flavonoid content than did bulbs. Amounts of eight elements (Cu, Mn, Zn, Fe, Ni, Pb, Cd and Cr) were also determined in the bulb and aerial part using atomic absorption spectroscopy. They contained higher Fe and Mn contents than other elements.

Reducing power and radical scavenging activity of four Parmeliaceae species

2010 ◽  
Vol 5 (6) ◽  
pp. 808-813 ◽  
Author(s):  
Gordana Stojanović ◽  
Igor Stojanović ◽  
Vesna Stankov-Jovanović ◽  
Violeta Mitić ◽  
Danijela Kostić
Keyword(s):  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenol Content ◽  
Significant Activity ◽  
Evernia Prunastri ◽  
Parmelia Sulcata ◽  
Potential Source ◽  
Flavoparmelia Caperata

AbstractThe methanol extracts of four Parmeliaceae lichens (Hypogymnia physodes, Evernia prunastri, Flavoparmelia caperata and Parmelia sulcata) were screened for antioxidant properties and total phenol content. The H. physodes extract was the most effective at reducing iron(III) and scavenging 1,1-diphenyl-2-picrylhydrazyl radicals, while the P. sulcata extract was the most effective in reducing molybdenum(VI) in an acidic medium. The E. prunastri and H. physodes extracts contained more Folin-Ciocalteu reagent reactive substances than the F. caperata and P. sulcata extracts. Significant activity of the H. physodes extract in DPPH and reducing Fe(III) assays suggest that this lichen can be considered as a potential source of antioxidants.

scholarly journals Biotransformation of soy whey into a novel functional beverage by Cordyceps militaris SN-18

2021 ◽  
Vol 3 (1) ◽  
Author(s):  
Yiqiang Dai ◽  
Jianzhong Zhou ◽  
Lixia Wang ◽  
Mingsheng Dong ◽  
Xiudong Xia
Keyword(s):  
Dna Damage ◽  
High Performance ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Essential Amino Acids ◽  
Cordyceps Militaris ◽  
Total Phenolic ◽  
Functional Beverages ◽  
Radical Scavenging Ability ◽  
Phenolic And Flavonoid

Abstract Soy whey, a liquid nutritional by-product of soybean manufacture, is rich in proteins, oligosaccharides and isoflavones. Soy whey can be used to produce functional beverages, instead of discarding it as a waste. In this study, unfermented soy whey (USW) and Cordyceps militaris SN-18-fermented soy whey (FSW) were investigated and compared for their physicochemical and functional properties by high performance liquid chromatography (HPLC) and DNA damage assay. Results show that C. militaris SN-18 fermentation could increase the contents of essential amino acids, total phenolic and flavonoid and isoflavone aglycones and eliminate the oligosaccharides in soy whey. Furthermore, C. militaris SN-18 could significantly enhance the ABTS radical scavenging ability, reducing power and ferric reducing power of soy whey, and its fermented products could prominently attenuate Fenton reaction-induced DNA damage. These findings indicate that soy whey can potentially be converted into a novel soy functional beverage by C. militaris SN-18 fermentation. Graphical abstract

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