The blood oxygen level dependent (BOLD) effect of in-vitro myoglobin and hemoglobin

The presence of deoxygenated hemoglobin (Hb) results in a drop in T2 and T2* in magnetic resonance imaging (MRI), known as the blood oxygenation level-dependent (BOLD-)effect. The purpose of this study was to investigate if deoxygenated myoglobin (Mb) exerts a BOLD-like effect. Equine Met-Mb powder was dissolved and converted to oxygenated Mb. T1, T2, T2*-maps and BOLD-bSSFP images at 3Tesla were used to scan 22 Mb samples and 12 Hb samples at room air, deoxygenation, reoxygenation and after chemical reduction. In Mb, T2 and T2* mapping showed a significant decrease after deoxygenation (− 25% and − 12%, p < 0.01), increase after subsequent reoxygenation (+ 17% and 0% vs. room air, p < 0.01), and finally a decrease in T2 after chemical reduction (− 28%, p < 0.01). An opposite trend was observed with T1 for each stage, while chemical reduction reduced BOLD-bSSFP signal (− 3%, p < 0.01). Similar deflections were seen at oxygenation changes in Hb. The T1 changes suggests that the oxygen content has been changed in the specimen. The shortening of transverse relaxation times in T2 and T2*-mapping after deoxygenation in Mb specimens are highly indicative of a BOLD-like effect.

Simultaneous Measurement of the BOLD Effect and Metabolic Changes in Response to Visual Stimulation Using the MEGA-PRESS Sequence at 3 T

The blood oxygen level dependent (BOLD) effect that provides the contrast in functional magnetic resonance imaging (fMRI) has been demonstrated to affect the linewidth of spectral peaks as measured with magnetic resonance spectroscopy (MRS) and through this, may be used as an indirect measure of cerebral blood flow related to neural activity. By acquiring MR-spectra interleaved with frames without water suppression, it may be possible to image the BOLD effect and associated metabolic changes simultaneously through changes in the linewidth of the unsuppressed water peak. The purpose of this study was to implement this approach with the MEGA-PRESS sequence, widely considered to be the standard sequence for quantitative measurement of GABA at field strengths of 3 T and lower, to observe how changes in both glutamate (measured as Glx) and GABA levels may relate to changes due to the BOLD effect. MR-spectra and fMRI were acquired from the occipital cortex (OCC) of 20 healthy participants whilst undergoing intrascanner visual stimulation in the form of a red and black radial checkerboard, alternating at 8 Hz, in 90 s blocks comprising 30 s of visual stimulation followed by 60 s of rest. Results show very strong agreement between the changes in the linewidth of the unsuppressed water signal and the canonical haemodynamic response function as well as a strong, negative, but not statistically significant, correlation with the Glx signal as measured from the OFF spectra in MEGA-PRESS pairs. Findings from this experiment suggest that the unsuppressed water signal provides a reliable measure of the BOLD effect and that correlations with associated changes in GABA and Glx levels may also be measured. However, discrepancies between metabolite levels as measured from the difference and OFF spectra raise questions regarding the reliability of the respective methods.

Functional Magnetic Resonance Imaging: From Acquisition to Application

Functional magnetic resonance imaging (fMRI) is a technique that exploits magnetic resonance imaging (MRI) to detect regional brain activity through measurement of the hemodynamic response that is coupled to electrical neuronal activity. The most common fMRI method detects blood oxygen level dependent (BOLD) contrast. The BOLD effect represents alteration in the ratio of deoxygenated to oxygenated hemoglobin within brain tissue following neuronal activity. Alterations in this hemoglobin ratio result from changes in cerebral oxygen extraction, cerebral blood flow, and cerebral blood volume that occur in response to neuronal activity. The small, but detectable, change in magnetics resonance signal intensity is due to the sensitivity of magnetic resonance (MR) images to the paramagnetic deoxygenated state of hemoglobin that is the basis of contrast in fMRI applications. This review describes the physical and physiological bases of the MR signal, the principle of the BOLD effect, technical issues related to fMRI implementation, and fMRI experimental design. Research and clinical applications of fMRI are presented, including the use of fMRI in neurosurgical planning. Since it provides an individualized map of brain function, fMRI enables accurate localization of eloquent brain regions prior to surgery, allowing assessment of surgical risk and prognosis, as well as planning surgical approach.