Pengelolaan Laboratorium Kimia Kimia di SMAN 1 Kupang Nusa Tenggara Timur (NTT)

This PKM is conducted at SMA Negeri I Kupang, which is located at JLN. Cak Doko, No. 59, Oetete Village, Oebobo District, Kupang City, NTT Province. The output in this PKM is a well-organized chemical laboratory, producing types of laboratory administration, analyzing the increase in teacher knowledge and skills regarding laboratory administration and governance. The result of this PKM activity was an increase in the value of the ability of teachers in laboratory management and administration which had an impact on the conditions of the chemistry laboratory at SMAN 1 Kupang on 5 governance indicators indicated by N-Gain values ​​and criteria consecutively as follows, planning indicator 0, 62 (moderate), organizing indicator 0.33 (moderate), administration indicator 0.78 (high), laboratory instrument arrangement indicator 1 (high), laboratory material structuring indicator 0.93 (high), and safety indicator 0.67 ( moderate). Thus it can be concluded that this PKM activity can improve teachers' knowledge and skills regarding laboratory governance.

Implementasi Quick Response (QR) Code Pada Dokumen Instruksi Kerja Alat Laboratorium Kimia

Work instructions laboratory equipment is one of the documents that must be available in the laboratory. This document provides information on the operation steps of the device correctly. A laboratory manager usually prints the document on A4 size paper and installs it on a part of the tool to facilitate access to the document. The treatment makes the work instruction document break down quickly and is not suitable to be applied to small sized tools. Therefore, it is necessary to manage the work instruction document for easy access, efficiency in the use of documents, and to improve the security of document storage. In this case, the QR Code can be used as a solution to the problem. QR Code is able to store documents and URL links in a small barcode image so that it can be use more efficient. Making a QR Code is preceded by storing documents at https://drive.google.com. The URL link at https://drive.google.com is entered into the QR-Code Studio 1.0 application and converted into a barcode. Barcodes are then printed and installed on each laboratory instrument. Scanning QR Code using a smartphone application in the form of a QR-Code Scanner. The results of the barcode scanned are the form of scripts for laboratory work instructions

A survey of clinical laboratory instrument verification in the UK and New Zealand

Background Clinical laboratory instrument verification testing is often an accreditation requirement. However, it is not known what verification procedures are in routine use or how often the process identifies problems which need addressing prior to testing clinical samples. Objective To investigate which standards are currently being used for laboratory verification in UK and New Zealand (NZ) clinical laboratories and to help establish if the activity justifies the effort required. Methods A survey of verification of clinical laboratory instrumentation was distributed to members of the Association for Clinical Biochemistry and Laboratory Medicine and New Zealand Institute of Medical Laboratory Scientists. The survey consisted of questions on the verification elements used and whether acceptance criteria were met. Results Nineteen of 72 (26%) of responders only used organization-developed protocols for verification, 20/72 (28%) solely used national/international guidelines, while 16/72 (22%) used a combination. Manufacturers’ claims were partly or entirely used as acceptance criteria for imprecision (89%), accuracy (64%) and analytical measuring range (94%), with these being met on 61%, 67% and 93% of occasions, respectively. For patient comparison and linearity, acceptance criteria were met by 71% and 91%. Only 27–36% undertook any troubleshooting before accepting a failed component of verification. Conclusions Laboratories in the UK and NZ are currently using a variety of verification standards and acceptance criteria for instrument verification. It is common for instruments to fail, especially following the assessment of imprecision and accuracy. While this suggests the process is warranted, only a minority address failed elements before accepting verification.

Anomaly Detection in Paleoclimate Records Using Permutation Entropy

Permutation entropy techniques can be useful for identifying anomalies in paleoclimate data records, including noise, outliers, and post-processing issues. We demonstrate this using weighted and unweighted permutation entropy with water-isotope records containing data from a deep polar ice core. In one region of these isotope records, our previous calculations (See Garland et al. 2018)revealed an abrupt change in the complexity of the traces: specifically, in the amount of new information that appeared at every time step. We conjectured that this effect was due to noise introduced by an older laboratory instrument. In this paper, we validate that conjecture by reanalyzing a section of the ice core using a more advanced version of the laboratory instrument. The anomalous noise levels are absent from the permutation entropy traces of the new data. In other sections of the core, we show that permutation entropy techniques can be used to identify anomalies in the data that are not associated with climatic or glaciological processes, but rather effects occurring during field work, laboratory analysis, or data post-processing. These examples make it clear that permutation entropy is a useful forensic tool for identifying sections of data that require targeted reanalysis—and can even be useful for guiding that analysis.

Size-exclusion chromatography small-angle X-ray scattering of water soluble proteins on a laboratory instrument

Coupling of size-exclusion chromatography with biological solution small-angle X-ray scattering (SEC-SAXS) on dedicated synchrotron beamlines enables structural analysis of challenging samples such as labile proteins and low-affinity complexes. For this reason, the approach has gained increased popularity during the past decade. Transportation of perishable samples to synchrotrons might, however, compromise the experiments, and the limited availability of synchrotron beamtime renders iterative sample optimization tedious and lengthy. Here, the successful setup of laboratory-based SEC-SAXS is described in a proof-of-concept study. It is demonstrated that sufficient quality data can be obtained on a laboratory instrument with small sample consumption, comparable to typical synchrotron SEC-SAXS demands. UV/vis measurements directly on the SAXS exposure cell ensure accurate concentration determination, crucial for direct molecular weight determination from the scattering data. The absence of radiation damage implies that the sample can be fractionated and subjected to complementary analysis available at the home institution after SEC-SAXS. Laboratory-based SEC-SAXS opens the field for analysis of biological samples at the home institution, thus increasing productivity of biostructural research. It may further ensure that synchrotron beamtime is used primarily for the most suitable and optimized samples.