Substrate-borne vibrations used during acoustic communication and the existence of courtship songs in some species of the genus Anaxipha (Saussure) (Orthoptera: Trigonidiidae: Trigonidiinae)

Anaxipha (Saussure, 1874) are small, swordtail crickets found in much of eastern North America. Many species within the genus Anaxipha were only recently described and their calling songs characterized. However, little is known about their courtship songs or use of substrate-borne communication (drumming). This study is the first documentation of the existence of courtship songs and substrate-borne vibrational communication in the genus. Courtship songs and substrate-borne vibrational communication were first detected in the following species: Anaxipha exigua (Say, 1825), A. tinnulacita Walker & Funk, 2014, A. tinnulenta Walker & Funk, 2014, and A. thomasi Walker & Funk, 2014. When in the presence of a conspecific female, males of all four species perform courtship songs that are distinctly different in pattern of echeme delivery and syllable details compared to their respective calling songs. Additionally, males of all four species exhibited drumming behavior during courtship singing and variably during calling songs. Examination of video recordings of males drumming during courtship singing showed that they are apparently using the sclerotized portion of their mandibles to impact the substrate on which they are perched to create vibrations. Courtship song and drumming bout characteristics were statistically different among the four species studied here, although A. tinnulacita and A. tinnulenta were similar in some measurements. Drumming during calling songs was common only in A. tinnulacita, where drumming occurs predominately during the first forty percent and last twenty percent of the long echemes of calling songs. Additional study is needed to further explore the use of substrate-borne vibrational communication in this genus.

A bilateral gynandromorph of Discoelius dufourii (Hymenoptera, Vespidae, Zethinae): morphology and mating behaviour

A gynandromorph is an organism combining both female- and male-type tissues. While the vast majority of studies have described the morphology of gynandromorphs, their sexual behaviours remain under-explored. We studied a predominantly bilateral gynandromorph specimen of the predatory wasp Discoelius dufourii (Hymenoptera: Vespidae: Zethinae) reared from a trap-nest. In addition to describing the morphology, we explored the gynandromorph’s response to a conspecific female, comparing it with the behavioural interactions between normal males and females of D. dufourii and the phylogenetically related D. zonalis. For most specific behavioural elements, males of both Discoelius species exhibited a greater frequency and a higher probability of occurrences of the behaviour than did females. However, aggression and plopping behaviours were prevalent in females. The gynandromorphic specimen demonstrated behavioural activities similar to those of a male, including a relatively frequent mate body antennation and an attempt to mount a female. However, it approached the female less frequently in comparison to conspecific normal males, and it did not demonstrate substrate antennation and jumping, typical of Discoelius male mating behaviour. Based on the parameters of the nest cell, the probability of the development of the gynandromorphic specimen from an unfertilized (male) egg was higher than the probability of development from a fertilized (female) egg.

Sexual Choice in Males of the Triatoma brasiliensis Complex: A Matter of Maintenance of the Species or Genetic Variability?

Background: Members of the Triatoma brasiliensis complex can produce experimental and natural hybrids. Crossing experiments performed in the laboratory, with several combinations between species of that complex, revealed a gradient of reproductive affinities among them. However, little is known about the reproductive males’ choices when they have the possibility of copulating with females of different species, including interspecific and conspecific females, at the same time. In this unprecedented experiment, the sexual choices of the T. brasiliensis complex and Triatoma infestans males were observed. Methods: Virgin males and females of T. b. brasiliensis, T. sherlocki, and T. infestans, and females of T. juazeirensis were used. The experiment was developed in an arena in which one male, one conspecific female, and two non-conspecific females were observed for 15 minutes. The following variables of mating behavior were observed: the male’s choice for a female; displacement time (the time it took the male to move from its stall until it reached the female); the copula itself (number of attempts and its occurrence); and the type of rejection of copula by the female. Results: Males of T. sherlocki were faster in finding the females (conspecific and non-conspecific) than T. b. brasiliensis and T. infestans. Males of T. b. brasiliensis and T. sherlocki were able to copulate with conspecific females and other female species: T. infestans and T. b. brasiliensis/T. juazeirensis, respectively. While T. infestans copulated with conspecific females, and T. juazeirensis and T. b. brasiliensis females. Conclusion: The results suggest that the choice for the copula is not always towards conspecific females. In fact, the males of the three different species tested were able to copulate with their conspecific females and also with other female species, which may induce the formation of hybrids and greater genetic diversity. These findings pose new challenges to the understanding of the reproductive behavior and the evolutionary aspects of the Triatominae. Therefore, in areas of sympatry, if no ecological barriers exist, there is the possibility of natural hybridization, which might reflect in the epidemiological risks since the species studied occur in endemic areas for the Chagas disease.

Notes on Advertisement Calls Playback by Three Species of Sarawakian Frogs

Male and female frogs respond differently towards advertisement calls. The fittest call will be chosen by the conspecific female to produce progenies, means that call from male to female is to ensure the survival of the species. The objective is to observe the response of both male and female frogs by playing the advertisement call to another male or female of the same species at their breeding site. The advertisement calls were recorded manually and were replayed using a speaker with built-in amplifier. The frog’s responses were then recorded in video form. Ambient temperatures were taken using data logger. The calls were analyzed to describe call characteristics. From the acoustic playback, both male and female Pulchrana glandulosa responded towards the calls. The male produces a crying-like sound while approaching playback source meanwhile the female produces a small “wik” sound. Male Pulchrana baramica responded by straining their calls and approaches the playback source. For male Kurixalus appendiculatus, the individuals responded by moving towards the sound source without calling. Results show that male of different species and male and female of the same species react differently towards playback calls. This shows that the breeding call recorded can be recognized by other frogs when they were played on amplifier. Keywords: Advertisement calls, call characteristics, playback recording, Sarawak frogs.

FIRST RECORD OF NECROPHILIA IN THE BAJA CALIFORNIA SPINY LIZARD, Sceloporus zosteromus (Phrynosomatidae)

Necrophilia is a reproductive behavior were a live animal attempts to mate with a dead conspecific. This behaviour has been reported in several lizard species. Here, we report the first case of necrophilia of an adult male Sceloporus zosteromus with a dead conspecific female in northwestern Baja California, Mexico.

Hemipenes eversion behavior: a new form of communication in two Liolaemus lizards (Iguania: Liolaemidae)

Males of several animals have intromittent organs and may use these in a communicative context during sexual or intrasexual interactions. In some lizards, hemipenes eversion behavior have been observed, and the aim of this study is to find out whether this behavior is functionally significant under a communicative approach. Here, we investigated the eversion of hemipenes in the Light Blue Lizard (Liolaemus coeruleus Cei and Ortiz-Zapata, 1983) and in the Valley Lizard (Liolaemus quilmes Etheridge, 1993) by filming the response of male focal lizards in different experimental settings: (i) an agonistic context, i.e., with a conspecific male, (ii) a sexual context, i.e., with a conspecific female, and (iii) a control treatment, i.e., without a treatment lizard. In both species, focal lizards showed this behavior only in an agonistic context, with interspecific differences as follows. Liolaemus coeruleus has longer times until eversion and dragging of hemipenes; however, it has shorter time of eversion and exposition of the hemipenes. Liolaemus quilmes has the opposite pattern compared with L. coeruleus. These indicate that eversion of the hemipenes can act as a visual display and as a signal of aggressive behavior towards conspecific rival males. The present study offers a new behavioral perspective on the use of masculine genitalia in lizards.

Double meaning of courtship song in a moth

Males use courtship signals to inform a conspecific female of their presence and/or quality, or, alternatively, to ‘cheat’ females by imitating the cues of a prey or predator. These signals have the single function of advertising for mating. Here, we show the dual functions of the courtship song in the yellow peach moth, Conogethes punctiferalis , whose males generate a series of short pulses and a subsequent long pulse in a song bout. Repulsive short pulses mimic the echolocation calls of sympatric horseshoe bats and disrupt the approach of male rivals to a female. The attractive long pulse does not mimic bat calls and specifically induces mate acceptance in the female, who raises her wings to facilitate copulation. These results demonstrate that moths can evolve both attractive acoustic signals and repulsive ones from cues that were originally used to identify predators and non-predators, because the bat-like sounds disrupt rivals, and also support a hypothesis of signal evolution via receiver bias in moth acoustic communication that was driven by the initial evolution of hearing to perceive echolocating bat predators.

Validation and new synonymies proposed for Cheiracanthium species from South and Southeast Asia (Araneae, Clubionidae)

The primary types of Cheiracanthium species from South and Southeast Asia were examined. Cheiracanthium insigne O.P.–Cambridge, 1874 is considered a senior synonym of Eutittha gracilipes Thorell, 1895 (male). Eutittha truncata Thorell,1895 (female) is not a conspecific female of E. gracilipes, provisionally threated as a separate species and proposed hereas a valid taxon because it does not resemble the female of C. insigne. Cheiracanthium rupicolum (Thorell, 1897) is re-garded as a senior synonym of C. gyirongense Hu & Li, 1987. The Himalaya form of C. triviale (Thorell, 1895), whichwas mentioned and illustrated in Gravely (1931), was misidentified and belongs to C. rupicolum. Cheiracanthium insu-lanum (Thorell, 1878) bears strong resemblance to that of C. melanostomum (Thorell, 1895); they probably belong to the same species.

An unusual occurrence of multiparasitism by two genera of Strepsiptera (Insecta) in a mango leafhopper Idioscopus clypealis (Lethierry) (Hemiptera: Cicadellidae) in the Philippines

The mango leafhopper Idioscopus clypealis (Lethierry) (Hemiptera: Cicadellidae) from the Philippines is parasitized bytwo genera of Strepsiptera, Halictophagus and Callipharixenos. A redescription of the male Halictophagus fulmeki(Hofeneder), a new record and description of its conspecific female and a key to Halictophagus in the Philippines aregiven, and a new species of female, Callipharixenos philippines sp. n. is described. The inclusion of the family Callipharixenidae as a subfamily Callipharixeninae within the family Halictophagidae is discussed.