Comparative two-dimensional fluorescence gel electrophoresis

Comparative two-dimensional gel electrophoresis (CoFGE) is a special version of two-dimensional polyacrylamide GE (2D-PAGE) and related to difference GE (2D-DIGE). It provides reproducibility and standardisation for 2D-PAGE by introducing a reference to the experiment. CoFGE uses different fluorescent labels to distinguish analyte and a marker protein mixture. The method allows in silico correction of the assignment of gel-separated proteins based on the co-run references, which form a grid of landmarks across the entire gel. The variability of spot coordinates is reduced to ~1% error and data can thus be compared to results generated independently with the same method. In this way, searchable repositories of gel-separated proteins become feasible. In addition, the CoFGE experimental principle can be used for protein quantification by applying the proteins of the marker grid in different concentrations. Here we present the protocol for conducting a CoFGE experiment, which takes about 2 days to complete for a technician skilled in GE.

Using the Few-Group Approximation for Calculating Some Neutron-Physical Characteristics of VVER-1000 Core by Means of the MCU Monte-Carlo Code

The main purpose of this work is to study the possibility of using the few-group approximation for calculation of some neutron-physical characteristics of VVER-1000 core by means of special version of MCU code. The Monte-Carlo method for VVER-1000 core neutron-physical characteristics calculation using the few-group approximation with an estimate of neutron cross sections “by location“ was provided and tested in this research. The reduction of calculation time due to the transition from a pointwise model of representation of cross sections to the few-group approximation and methodical error of this approach were evaluated. Optimal number of energy groups was determined. It was found that consideration of the scattering anisotropy leads to a significant decrease in methodical error. Ways of further reduction of methodical error were worked out.

A standard form in (some) free fields: How to construct minimal linear representations

We describe a standard form for the elements in the universal field of fractions of free associative algebras (over a commutative field). It is a special version of the normal form provided by Cohn and Reutenauer and enables the use of linear algebra techniques for the construction of minimal linear representations (in standard form) for the sum and the product of two elements (given in a standard form). This completes “minimal” arithmetic in free fields since “minimal” constructions for the inverse are already known. The applications are wide: linear algebra (over the free field), rational identities, computing the left gcd of two non-commutative polynomials, etc.

Editorial

We are all homebound in a global village, owing to the novel coronavirus or Covid-19. This is indeed a special version of Ushus-Journal of Business Management. I wish safety and good health to one and all. Let almighty bless us.This issue is quite unique in terms of coverage. It covers wide variety of quantitative finance aspects.

Economic Exceptionalism? Justice and the Liberal Conception of Rights

Are political and economic rights equally basic? This is one of the main issues liberal egalitarians and classical liberals disagree about. The former think political rights should be more strongly protected than economic ones; classical liberals thus accuse them of an unjustified and politically biased ‘economic exceptionalism’. Recently, John Tomasi has developed a special version of this challenge, which is targeted against Murphy and Nagel’s account of the relationship between property rights and just taxation. In this paper, I analyze this challenge, and provide an account of its limitations. Tomasi’s strategy to drive Murphy and Nagel’s account into an overgeneralization problem brings to light that liberals weren’t guilty of any kind of economic exceptionalism in the first place. However, this also shows that classical liberalism and libertarians do not disagree as much as it might seem.

Myth about Tibet in Chinese exposition

The paper is sanctified to the analysis of cultural text of Tibet. Under cultural text is understood correlation of traditional presentations, or myths about what a culture of one or another country is for her external recipients, and real matter-position. A myth about Tibet contains the row of ideas about the wonderful place of force, giving possibility of brightening and finding of authentic life. He is caused to life by the unique geographical location of Tibet, his unusual history and his culture related to the special version of buddhism, interest in that today grows in the whole world. The real matter-position is in Tibet, however, appears very distant from that draws a myth. It is certain as complete destruction of the traditional Tibetan culture and substitution her modern version of the Chinese culture.

Rupert Schreibers Deskriptionsthese

This paper deals with the ‚description-thesis‘ (R. Schreiber 1962) according to which normative or prescriptive legal statements are reducible to, or translatable into, descriptive propositions. The arguments Schreiber produced in support of his thesis are briefly presented, followed by a discussion of the thesis against the background of Hume’s law and G. E. Moore’s open-question argument. In order to broaden the perspective, some other philosophical views are examined; emphasis is placed on how the is-ought relation was conceived, in conflicting ways, by Hans Kelsen and Friedrich Müller respectively, as compared to the current state of discussion, and what are, according to Ernst Tugendhat and Christoph Möllers, the constituents of (social) norms. As a result, the paper suggests that the description-thesis can not be maintained in its entirety. A special version is presented which seems to respond to the criticism discussed while implying at least part of what Schreiber’s original version claimed.

Methodology for studying A.T. Tvardovsky’s lyrical cycle “In Mother’s Memory” in the 11th grade

This article is one of a series of the author’s works devoted to the study of lyrical cycles in high school lessons in Literature. The described methodology is based on the textual concept worked out by E.A. Sterjopulu that is adapted to the school environment. According to this concept, the lyrical cycle is considered as a system of poetic texts that has a number of features: interrelatedness, inter-complementation, valency and cohesiveness. Based on the listed literary concepts, the author of the article builds the logic of work with the cycle at the lesson, examines and describes several ways of studying the lyrical cycle in literature classes at school: 1) linear, or “following the author”, when the writing elements included in the cycle are analyzed sequentially; 2) “concentric”, when one, as a rule, the central (dominant) poem is examined in detail, and semantic threads extend from it to the others; 3) a special version of the “concentric” logic, when the image (images) or motive (motives) that consolidates the poems is in the focus of attention. This article presents the way these models can be implemented in the course of studying A.T. Tvardovsky’s cycle "In Мother’s Memory". The paper presents a system of questions and tasks that organize the activities of the teacher and students in the classroom, as well as detailed comments on them. The result of such lessons is that students are able to understand the cycle as a holistic unit that has a meta-meaning. This, in its turn, contributes to the development not only of the competence in reading, but also of the systematic thinking.

Turkic and Slavic population of Siberia: identity, culture, religion

Siberia is an area of active interethnic, interreligious and intercultural contacts the most of which take place between Slavic and Turkic peoples. Despite the extraordinary diversity of their traditional and everyday culture, they have a lot in common in mentality and culture which got all-Siberian features, formed on a basis of the Russian language and culture. The religious, ethnic, linguistic and ethno-cultural identities of one person are often not similar, as well as self-determination and the real situation. We can conclude that Siberia is a place where a special version of the Eurasian identity is formed.