Optimization of Bacterial Concentrate Media for Lactobacillus Sakei

Two strains of lactic acid bacteria were selected for the study (Lactobacillus sakei Lsk-45andLactobacillus sakeiDSM 20017). Bacterial media, based on rice and rice flour, were tested as an alternative to media based on whey. A comparison of the different types of media showed that there was better growth of the selected strains on themedium based on rice flour.Statistical analyses, including factorial experiments and response surface analyses, were used to optimizethe composition of the bacterial medium for Lactobacillus sakei propagation. Bacteriological peptone and rice flour were found to be good growth factors for Lactobacillus sakei.For Lactobacillus sakei Lsk-45,better growth was obtained with the use of 7.75-10 g/L of peptone and 57.5-75 g/L of rice flour. For Lactobacillus sakei DSM 20017, better growth was obtained with the use of 7-10 g/L of peptone and 40-75 g/L of rice flour. Keywords: starters, media, Lactobacillus sakei, fermentation,fish products, bacterial strains, lactic acid bacteria

Some notes on planning plant protection research in block designs with nested rows and columns

Summary Factorial experiments in block designs with nested rows and columns are described with suggestions about how they should be planned. In such experiments the importance of interaction and hidden replication are emphasized. Such experiments are carried out on heterogeneous experimental material. Thus, it is reasonable to seek a design that can withstand the loss of observations. The robustness of a block design with nested rows and columns against the loss of whole blocks is presented, along with examples of such designs.

Sacrifice for (not) buying: how to measure sacrifice in the consumer context

This paper describes what the sacrifice is for consumption and proposes a scale to measure the sacrifice for (not) buying products. A multimethod approach was applied to achieve the proposed objectives. Initially, three qualitative studies were carried out (two focus groups and one interview with experts). Then, three quantitative studies were conducted (two online and a survey), and finally, two factorial experiments, 2x2 were developed. The act of sacrifice was understood as an exchange process in which some kind of benefit is sought, a fact that also consolidates the definition of sacrifice presented as the willingness to give up something that has value (monetary or not) in order to obtain some benefit (emotional and/or material) of greater importance. Furthermore, the existence of positive and negative elements in the sacrifice for (not) buying products, described as valence and instrumentality, respectively, was identified. It was also found that different levels of valence and instrumentality affect purchase intent. The elements that comprise the sacrifice for (not) buying products were presented, highlighting a definition that can reduce the doubts about what sacrifice is in the context of consumption, as well as how to measure it.

Barriers to Building More Effective Treatments: Negative Interactions Among Smoking-Intervention Components

Meaningfully improved mental and behavioral health treatment is an unrealized dream. Across three factorial experiments, inferential tests in prior studies showed a pattern of negative interactions, suggesting that better clinical outcomes may be obtained when participants receive fewer rather than more intervention components. Furthermore, relatively few significant main effects were found in these experiments. Modeling suggested that negative interactions among components may account for these patterns. In this article, we evaluate factors that may contribute to such declining benefit: increased attentional or effort burden; components that produce their effects via the same capacity-limited mechanisms, making their effects subadditive; and a tipping-point phenomenon in which people near a hypothesized tipping point for change will benefit markedly from weak intervention and people far from the tipping point will benefit little from even strong intervention. New research should explore factors that cause negative interactions among components and constrain the development of more effective treatments.

THE EFFECTIVE VOLUME OF WATER IN SEVERAL TYPES OF SOIL FOR THE GROWTH OF Mucuna bracteata

The aim of this research was to determine the effective volume of water in several types of soil for the growth of Mucuna bracteata. This research was conducted at the Education and Research Garden (KP-2) of the Stiper Agricultural Institute located in Maguwoharjo, Sleman, DIY. This study uses factorial experiments arranged in Completely Randomized Design (CRD) consisting of two factors and five replications. The first factor was the volume of water spray/day/plant consisting of four volume levels, namely: 50 ml, 100 ml, 150 ml and 200 ml. The second factor was the type of soil consisting of three types of soil, namely : regosol, latosol and grumusol. The results of the research data were analyzed using variance (Analysis of variance) at a real level of 5%. Data that is significantly different is continued with Duncan's multiple distance test or DMRT (Duncan multiple range test) at a real level of 5%. The results showed that there was no good combination of the volume of water and soil type on the growth of Mucuna bracteata. The volume of 50 ml / plant water was sufficient to produce good Mucuna bracteata plant growth. Mucuna bracteata plants can grow in regosol, latosol, and grumusol soil types.Keywords: volume, water, soil type, Mucuna bracteata

Research Strategies to Discern Active Psychological Therapy Components: A Scoping Review

Some clinical scientists are shifting from research on complete named therapy protocols to a more elemental approach—research on specific therapy components that contribute to therapy goals. To characterize and evaluate this emerging field, we systematically searched PsycINFO and Medline for studies evaluating therapy components. We identified 208 studies. In a scoping review, we map, explain, and critically appraise the seven research strategies employed: (a) expert opinion, (b) shared components, (c) associations between the presence of components and therapy effects, (d) associations between fidelity to components and therapy effects, (e) microtrials, (f) additive and dismantling trials, and (g) factorial experiments. Our examination reveals a need for (a) renewed emphasis on experimental trials (vs. meta-analyses testing associations less rigorously), (b) expanded efforts to locate components within the emerging fields of process-based and principle-guided psychotherapy, and (c) a shift from innovative stand-alone studies to development of a coherent science of therapy components.