Physiological Response and Proteomics Analysis of Reaumuria soongorica Under Salt Stress

Soil salinity can severely restrict plant growth. Yet Reaumuria soongorica can tolerate salinity well. However, large-scale proteomic studies of this plant’s salinity response have yet to reported. Here, R. soongorica seedlings (4 months old) were used in an experiment where NaCl solutions simulated levels of soil salinity stress. The fresh weight, root/shoot ratio, leaf relative conductivity, proline content, and total leaf area of R. soongorica under CK (0 mM NaCl), low (200 mM NaCl), and high (500 mM NaCl) salt stress were determined. The results showed that the proline content of leaves was negatively correlated with salt concentration. With greater salinity, the plant fresh weight, root/shoot ratio, and total leaf area increased initially but then decreased, and vice-versa for the relative electrical conductivity of leaves. Using iTRAQ proteomic sequencing, 47, 177, 136 differentially expressed proteins (DEPs) were identified in low-salt vs. CK, high-salt vs. control, and high-salt vs. low-salt comparisons, respectively. A total of 72 DEPs were further screened from the groups, of which, 34 DEPs increased and 38 DEPs decreased in abundance. These DEPs are mainly involved in translation, ribosomal structure, and biogenesis. Finally, 21 key DEPs (SCORE value ≥ 60 point) were identified as potential targets for salt tolerance of R. soongolica. By comparing the protein structure of treated vs. CK leaves under salt stress, we revealed the key candidate genes underpinning R. soongolica’s salt tolerance ability. This works provides fresh insight into its physiological adaptation strategy and molecular regulatory network, and a molecular basis enhancing breeding, under salt stress conditions.

miRNA–mRNA Integrated Analysis Reveals Roles for miRNAs in a Typical Halophyte, Reaumuria soongorica, during Seed Germination under Salt Stress

MicroRNAs (miRNAs) are endogenous small RNAs that play a crucial role in plant growth, development, and environmental stress responses. Reaumuria soongorica is a typical halophyte that is widely distributed in saline–alkali desert regions. Under salt stress, R. soongorica can complete germination, a critical biological process in the life cycle of seed plants. To identify miRNAs and predict target mRNAs involved in seed germination during salt stress, nine small-RNA libraries were constructed and analyzed from R. soongorica seeds treated with various concentrations of NaCl. We also obtained transcriptome data under the same treatment conditions. Further analysis identified 88 conserved miRNAs representing 25 defined families and discovered 13 novel miRNAs from nine libraries. A co-expression analysis was performed on the same samples to identify putative miRNA–mRNA interactions that were responsive to salt stress. A comparative analysis of expression during germination under 273 (threshold) and 43 mM (optimal) NaCl treatments identified 13 differentially expressed miRNAs and 23 corresponding target mRNAs, while a comparison between 43 mM NaCl and non-salt-stress conditions uncovered one differentially expressed miRNA and one corresponding target mRNA. These results provide basic data for further study of molecular mechanisms involved in the germination of salt-stressed R. soongorica seeds, and also provide a reference for the improvement of salt tolerance during plant germination.

Evapotranspiration of xerophytic shrub Salsola passerina and Reaumuria soongorica in an arid desert ecosystem of NW China

Understanding the actual evapotranspiration (ET) variation of the sparsely distributed xerophytic shrubs is crucial to accurately upscale community ET to ecosystem scale. Here we quantified the actual ET of two dominant xerophytic shrubs of the Tengger Desert in northwestern China, i.e. Salsola passerina and Reaumuria soongorica, by using four large weighing lysimeters. The results showed that with the increase in precipitation from 140 to 171 mm in the year 2015/2016, the daily mean evaporation (E) of the bare area, and ET of the single shrub communities of S. passerina, R. soongorica, and the associated shrub community (S. passerina + R. soongorica) increased 50, 60, 44, and 47%, respectively; correspondingly, the total E and ET increased 49, 61, 44, and 47%, respectively. The variation of soil moisture within 0–40 cm depth plays a vital role in regulating the E and ET. The new shoot length, as one of important parameters of the xerophytic shrub, was significantly exponentially related to the cumulative ET. From the long- and short-term perspective, event-based precipitation and wind speed are the dominant driving factors behind changes in E and ET, respectively. Relative humidity is the main influencing factor for E and ET after a large rainfall event within 8 days.