Management of Anemia of Chronic Disease: Beyond Iron-Only Supplementation.

Chronic diseases are characterised by cell’s autophagy and proteins disarrangement resulting in sarcopenia, hypoalbuminemia and hypo-haemoglobinaemia. Hypo-haemoglobinaemia couses worse prognosis independentely of the principal disease. Currently, the cornerstone of therapy of anaemia is iron supplementation, with or without erythropoietin for the stimulation of hematopoiesis. However, treatment strategies should incorporate the addition of heme, the principal biochemical constituent of haemoglobin. Heme synthesis follows a complex biochemical pathway. The limiting step of heme synthesis is D-ALA availability which, for its synthesis, requires Glycine and Succinil-CoA. Consequently, treatment of anaemia should not be based only on iron availability, but also on the availability of the molecules fundamental for heme synthesis. Therefore, an adequate clinical therapeutic strategy should integrate the standard iron infusion and the supply of essential amino acids and vitamins involved in the heme synthesis. We report preliminary data in selected elderly anaemic patients with congestive heart failure (CHF) and catabolic disarrangement, who, in addition to standard iron therapy, received personalized therapy with essential-AAs and vitamins involved in the maintenance of heme. Notably, such individualized therapy resulted in a significant increase in the serum concentration of haemoglobin after 30 days of treatment compared to standard iron therapy.

Biochemical Analysis of Organic Acids and Soluble Sugars in Wild and Cultivated Pomegranate Germplasm Based in Pakistan

Pomegranate is famous for its health benefiting chemical and biochemical constituent compounds. The present study was undertaken to characterize pomegranate germplasm for its various fruit traits, acids, and sugar profiling through high performance liquid chromatography (HPLC) analysis. Among 11 detected acids and 8 sugars, citric acid and fructose were predominant in 18 domestic and 5 wild genotypes, respectively. Fruit weight, aril weight and wood portion index (WPI) were ranged from 15.82% to 24.42%, 10.99% to 113.78%, and 2.39% to 17.25%, respectively. Genotypes were grouped as sweet, sweet–sour, sour–sweet, and sour based on citric acid contents. Lactic acid and pyruvic acid showed the highest correlation (r = 0.92), however, sour and sweet genotypes had strong association for acids and sugars, respectively. Straddling of dendrogram showed the flow of genetic material in a cultivated location with wild and cultivated pomegranates grouped in different classes, however, wild and sour landraces grouped in the same class with 71% similarity of traits. Based on the observations of the current study, it was concluded that selected wild and arid zones (Multan, Bahawalpur) genotypes are poor in nutrients (acid and sugars) quality, however, genotypes of Rahim-Yar-Khan, Muzafar Garh, and Khyber Pakhtunkhwa have a better composition of sugars and acids.

Phytohormone up-regulates the biochemical constituent, exopolysaccharide and nitrogen metabolism in paddy-field cyanobacteria exposed to chromium stress

The authors have withdrawn the journal submission associated with this preprint and requested that the preprint also be withdrawn.

Phytohormone up-regulates the biochemical constituent, exopolysaccharide and nitrogen metabolism in paddy-field cyanobacteria exposed to chromium stress

Current study deals with the assuaging effects of two phytohormones; indole acetic acid (IAA; 290 nM) and kinetin (KN; 10 nM) on growth, phycobiliproteins, status of nitrogen metabolism and biochemical constituents; protein, carbohydrate and exopolysaccharide contents in two diazotrophic cyanobacteria Nostoc muscorum and Anabaena exposed to chromium (CrVI) stress (100 µM and 150 µM). Chromium individually at both the tested doses expressively declined the growth, chlorophyll a to carotenoid ratio and contents of phycobiliproteins; phycocyanin (PC), allophycocyanin (APC), and phycoerythrin (PE). With distinctive impact on status of nitrogen metabolism chromium significantly reduced the nitrate (NO3—) and nitrite (NO2—) uptake rate and foremost decrease in nitrate and ammonia assimilating enzyme; nitrate reductase (NR), nitrite reductase (NiR), glutamine synthetase (GS), glutamate synthase (GOGAT) except glutamate dehydrogenase (GDH). However, beneath alike condition, exogenous application of IAA and KN exhibited noteworthy assuaging effects on growth-regulating parameters in both the paddy field cyanobacteria, which consummately occurred as a result of substantial decrease in Cr uptake and inducing signaling responses and also enhances the growth parameter i.e. nitrogen metabolism as a result of considerable lowering in Cr induced damaging effect on nitrogen metabolism and uptake rate, and the alleviating effect was more pronounced with the lower dose of Cr, efficient in N.muscorum than Anabaena.