Synaptic targets of photoreceptors specialized to detect color and skylight polarization in Drosophila

Color and polarization provide complementary information about the world and are detected by specialized photoreceptors. However, the downstream neural circuits that process these distinct modalities are incompletely understood in any animal. Using electron microscopy, we have systematically reconstructed the synaptic targets of the photoreceptors specialized to detect color and skylight polarization in Drosophila, and we have used light microscopy to confirm many of our findings. We identified known and novel downstream targets that are selective for different wavelengths or polarized light, and followed their projections to other areas in the optic lobes and the central brain. Our results revealed many synapses along the photoreceptor axons between brain regions, new pathways in the optic lobes, and spatially segregated projections to central brain regions. Strikingly, photoreceptors in the polarization-sensitive dorsal rim area target fewer cell types, and lack strong connections to the lobula, a neuropil involved in color processing. Our reconstruction identifies shared wiring and modality-specific specializations for color and polarization vision, and provides a comprehensive view of the first steps of the pathways processing color and polarized light inputs.

A visual pathway for skylight polarization processing in Drosophila

Many insects use patterns of polarized light in the sky to orient and navigate. Here we functionally characterize neural circuitry in the fruit fly, Drosophila melanogaster, that conveys polarized light signals from the eye to the central complex, a brain region essential for the fly's sense of direction. Neurons tuned to the angle of polarization of ultraviolet light are found throughout the anterior visual pathway, connecting the optic lobes with the central complex via the anterior optic tubercle and bulb, in a homologous organization to the 'sky compass' pathways described in other insects. We detail how a consistent, map-like organization of neural tunings in the peripheral visual system is transformed into a reduced representation suited to flexible processing in the central brain. This study identifies computational motifs of the transformation, enabling mechanistic comparisons of multisensory integration and central processing for navigation in the brains of insects.

A Novel Skylight Orientation Sensor for Autonomous Navigation

<a></a>The angle of the polarization (AOP) and the degree of polarization (DOP) of the scattered skylight are symmetrically distributed concerning the solar meridian. Based on the symmetry of the skylight polarization distribution pattern, this paper proposes a novel skylight orientation sensor consists of a camera, an S-waveplate, and a linear polarizer. The skylight orientation sensor is using the image polarization encoding capability of the S-waveplate and the linear polarizer to convert the skylight polarization information into the image’s symmetry axis extraction, which has the advantages of no resolution loss and instantaneous field of view error. The symmetry axis in the image is consistent with the solar meridian. Therefore, the angle between the solar meridian and the skylight orientation sensor reference axis can be obtained without calculating the polarization information, which is also beneficial for real-time performance. The angle measurement accuracy and uncertainty of the skylight orientation sensor are verified by numerical simulation and outdoor experiments. The results demonstrate that the skylight orientation sensor has good application potential in autonomous navigation.

A Novel Skylight Orientation Sensor for Autonomous Navigation

<a></a>The angle of the polarization (AOP) and the degree of polarization (DOP) of the scattered skylight are symmetrically distributed concerning the solar meridian. Based on the symmetry of the skylight polarization distribution pattern, this paper proposes a novel skylight orientation sensor consists of a camera, an S-waveplate, and a linear polarizer. The skylight orientation sensor is using the image polarization encoding capability of the S-waveplate and the linear polarizer to convert the skylight polarization information into the image’s symmetry axis extraction, which has the advantages of no resolution loss and instantaneous field of view error. The symmetry axis in the image is consistent with the solar meridian. Therefore, the angle between the solar meridian and the skylight orientation sensor reference axis can be obtained without calculating the polarization information, which is also beneficial for real-time performance. The angle measurement accuracy and uncertainty of the skylight orientation sensor are verified by numerical simulation and outdoor experiments. The results demonstrate that the skylight orientation sensor has good application potential in autonomous navigation.

Spatial orientation of social caterpillars is influenced by polarized light

Processionary caterpillars of Thaumetopoea pityocampa (in Europe) and Ochrogaster lunifer (in Australia) (Lepidoptera: Notodontidae) form single files of larvae crawling head-to-tail when moving to feeding and pupation sites. We investigated if the processions are guided by polarization vision. The heading orientation of processions could be manipulated with linear polarizing filters held above the leading caterpillar. Exposure to changes in the angle of polarization around the caterpillars resulted in corresponding changes in heading angles. Anatomical analysis indicated specializations for polarization vision of stemma I in both species. Stemma I has a rhabdom with orthogonal and aligned microvilli, and an opaque and rugged surface, which are optimizations for skylight polarization vision, similar to the dorsal rim of adult insects. Stemmata II-VI have a smooth and shiny surface and lobed rhabdoms with non-orthogonal and non-aligned microvilli; they are thus optimized for general vision with minimal polarization sensitivity. Behavioural and anatomical evidence reveal that polarized light cues are important for larval orientation and can be robustly detected with a simple visual system.

A visual pathway for skylight polarization processing in Drosophila

SUMMARYMany insects use patterns of polarized light in the sky to orient and navigate. Here we functionally characterize neural circuitry in the fruit fly, Drosophila melanogaster, that conveys polarized light signals from the eye to the central complex, a brain region essential for the fly’s sense of direction. Neurons tuned to the angle of polarization of ultraviolet light are found throughout the anterior visual pathway, connecting the optic lobes with the central complex via the anterior optic tubercle and bulb, in a homologous organization to the ‘sky compass’ pathways described in other insects. We detail how a consistent, map-like organization of neural tunings in the peripheral visual system is transformed into a reduced representation suited to flexible processing in the central brain. This study identifies computational motifs of the transformation, enabling mechanistic comparisons of multisensory integration and central processing for navigation in the brains of insects.

OPTICAL POLARIZED EFFECTS FOR QUANTITATIVE REMOTE SENSING

Polarization is one of the four basic physical properties of solar radiation. After the solar radiation reaches the surface of these media, it reflects, scatters or refracts, and exhibits different degrees of polarization. We use Rayleigh scattering model to get the simulation results of the sky polarization field. We use polarized fisheye camera to collect the sky polarization image, and calculate the distribution pattern of DOLP (degree of linear polarization) and AOLP (azimuth of linear polarization) of the skylight. The stability and gradual change of the degree of polarization in the zenith direction are verified, and the distribution law and daily change law of the degree of polarization in the sky are obtained. With the increase of the solar altitude angle, the degree of polarization will decrease. We also observed the skylight polarization in different weather conditions.