Reformulation of dairy cow diets based on rumen degradable protein and total digestible nutrient with varying levels on in vitro fermentability and digestibility

Rumen degradable protein (RDP) needs to be balanced with the adequacy of rumen undegradable protein (RUP) and energy for optimal microbial growth. Therefore, this study aimed to determine the optimal level of the RDP:RUP ratio and the energy level of dairy cattle rations using the in vitro method. The rumen inoculum used to carry out this research, was obtained from two bull rumen fistulated of Friesian Holstein. The treatments consisted of 3 levels of RDP:RUP ratio, namely 50:50, 55:45 and 60:40, while the energy levels consisted of total digestible nutrient (TDN) levels of 65.6% and 68.6%. The experimental design was a factorial randomized block, while data were analyzed using ANOVA and Duncan multi range’s test. The result showed the ratio of RDP:RUP had an effect on DMD, OMD, NH3, and rumen microbe. Furthermore, the higher TDN content increased significantly DMD, OMD, total VFA, and partial VFA. The increase in the microbial population was associated with a rise in total VFA and NH3 concentrations. This research concluded, the rations with RDP:RUP (60:40) ratio increased the population of bacteria and protozoa, while the availability of ammonia in the rumen, and the high level of TDN provided a higher supply of VFA, DMD, and OMD.

PENINGKATAN KUALITAS JERAMI PADI SEBAGAI PAKAN SAPI POTONG MELALUI AMONIASI MENGUNAKAN UREA DI DESA TIMBUOLO TENGAH PROVINSI GORONTALO

AbstrakAmoniasi jerami merupakan metode memperbaiki nutrisi jerami padi dengan merusak ikatan lignin-hemisellulosa sehingga mudah dicerna mikroba rumen. Tujuan kegiatan ini adalah meningkatkan pengetahuan dan keterampilan warga desa Timbuolo Tengah, Kabupaten Bone Bolango, Provinsi Gorontalo membuat jerami padi amoniasi. Metode yang digunakan pemberian teori dan simulasi kepada warga sasaran, dilanjutkan praktek langsung membuat jerami padi amoniasi. Kegiatan ini dilakukan 21 hari sejak persiapan, pelaksanaan pelatihan, evaluasi kualitas fisik dan kimia jerami padi amoniasi. Bahan pembuatan jerami padi amoniasi adalah jerami padi, urea, molases, Microbacter Alfaafa (MA)-11, dan air. Evaluasi kualitas fisik/organoleptik (warna, bau, tekstur, suhu, pH, dan keberadaan fungi) dan kimia (air, protein, lemak, serat kasar, Bahan Ekstrak Tanpa Nitrogen (BETN), dan abu) dilakukan di Laboratorium Nutrisi dan Makanan Ternak Universitas Hasanuddin. Selama kegiatan pelatihan, warga desa cukup antusias mengikuti semua tahap kegiatan sejak persiapan hingga evaluasi dengan tingkat capaian 100%. Hasil pengujian fisik diperoleh warna coklat muda/kecoklatan, bau amoniak, tekstur halus, Ph 7,8, Suhu 43,66 oC, dan ditemukan sedikit fungi/jamur. Hasil analisis proksimat diperoleh kadar air 8,09%, protein 5,65%, lemak 1,99%, serat kasar 33,60%, Bahan Ekstrak Tanpa Nitrogen (BETN) 31,66%, dan abu 27%.Kata Kunci: Jerami Padi, Amoniasi AbstractStraw ammoniation is method of improving rice straw nutritional quality by damaging lignin-hemisellulosa bond so it is more easily digested by rumen microbe. This activity aims to improve the knowledge and skill in making rice straw ammoniation of Middle Timbuolo villagers in Botupingge sub-district, Bone Bolango regency, Gorontalo province. The method used is giving theories and simulation to the target group members and continued by practicing directly in making ammoniation. This activity is carried out for 21 days with main ingredients are rice straw, urea, molasses, Microbacter Alfaafa (MA) -11,water. Physical quality evaluation was done after 21 days observing the color, odor, texture, temperature, pH, and the presence of fungi, and performing the proximate analysis of chemical evaluation (water, protein, fat, coarse fiber, Nitrogen Free Extract (NFE) and ash) at Nutrition and Feed Laboratory of Hasanuddin University. During the training, the villagers are enthusiastic with achievement level is 100%. The physical test results are color is brownish, smells like ammonia, has fine texture, PH 7.8, temperature 43.66 °C, and found little fungi. Proximate analysis results are water content 8.09%, protein 5.65%, fat 1.99%, coarse crude fiber 33.60%, Nitrogen Free Extract 31.66% and ash 27%. Keywords: Rice Straw, Ammoniation

Comparison of rumen microbe taxa among wild and domestic ruminants

Microbes that inhabit the rumen provide a diverse set of enzymes that degrade ingested plant material. Without microbes, ruminants could not survive. Our research has focused on identifying microbial taxa associated with environment and phenotypic traits of host animals in order to improve livestock production and efficiency. Rumen contents were collected from a total of 48 animals from five host species, both wild and domestic, consuming either a concentrate- or forage-based diet. The objectives of this study were to analyze the distribution of microbial taxa among host species and identify microbial taxa associated with host diet, species, and domestication status. DNA was extracted from rumen content and sequenced for identification of microbial taxa present within each host animal. The distribution of microbial taxa was similar among host species. Each host species contained a few highly abundant microbial taxa and many microbial taxa in lower abundance. As host species were added to the analysis, the total number of microbial taxa identified increased and the number of microbial taxa common among all host species decreased, supporting the existence of a core microbial community. In this study we identified 66, 73, and 23 microbial taxa that differed among host diet, species, and domestication status, respectively. While on farm implications are far from being offered, studies of the microbial community add to the breadth of knowledge and identify microbes associated with performance and efficiency of livestock.

Application of rumen microbial genome information to livestock systems in the postgenomic era

Sequencing the genomes of individual rumen microbes and determining the function of their encoded genes promises to transform our understanding of the microbiology of the rumen. The diversity and density of microbes in the rumen, and our inability to culture the majority of rumen microbes, limit current genome studies to only a small fraction of the microbes present in this environment. Nevertheless, genomes of fibre-degrading organisms are beginning to reveal a previously unexpected abundance of genes encoding glycosyl hydrolases and carbohydrate esterases, which could be used to enhance fibre digestion in the rumen. Additionally, genome sequencing of a rumen methanogen is identifying conserved genes within the methanogenic archaea that may serve as targets for their inhibition and therefore reduction of methane emissions from ruminants. The problem of rumen microbe culturability can be overcome by a new approach called metagenomics, in which microbial DNAs are extracted from rumen samples and sequenced independent of cultivation. In the future, sequencing individual genomes and metagenomic libraries is likely to capture much more of the microbial DNA in the rumen and, coupled with postgenomic studies on gene and protein expression, is likely to enhance our knowledge of the microbial component of ruminant digestion.

A study of nutritional status of Finnish reindeer (Rangifer Tarandus L.) in differents months: I. Composition and volume of the rumen microbiota

The rumen microbiota were studied in free-ranging semi-domestic reindeer in Finnish Lapland under the nutritional conditions obtaining at two different sampling times. Qualitative and quantitative investigations were made of the rumen ciliate fauna and quantitative investigations of the rumen bacterial flora. The volume coefficients for rumen ciliates obtained by Westerling (1970) and that for rumen bacteria obtained by Warner (1962) were used to obtain an indication of the volume of the rumen microbe mass in reindeer. The rumen samples were collected in connection with the round-up and slaughter of reindeer, being taken from 30 animals in December and 29 animals in March. The reindeer slaughtered in December had normal access to food, but those slaughtered in March had grazed on better pastures and received a supplementary feed of hay. The total number of ciliate cells was over six times as high in March as in December, the numbers being 1 182900 and 188 300 per ml rumen contents, respectively. The corresponding total numbers of bacterial cells were 9.65 x 109 in March and 6.65 x 109 in December. The reason for the statistically significantly (P < 0.01) higher numbers in March than in December is probably the better nutritional conditions of the herd slaughtered in March, not the time of the year. The ciliate fauna consisted of 19 different species, although not all the species were found in every sample. The percentage composition of the ciliate fauna did not vary considerably between the two sampling times. The volume of the total microbe mass constituted 8.2% of the rumen contents in March and 1.9 % in December, the average being 5.1 %. The proportion of the ciliate volume in the total microbe mass was clearly higher than that of the bacteria at both sampling times: 7.2 times as high in March and 1.7 times in December, the average being 4.7 times.