Association of the hOGG1 Ser326Cys polymorphism with gynecologic cancer susceptibility: a meta-analysis

The association between the hOGG1 Ser326Cys polymorphism and gynecologic cancer susceptibility is inconclusive. We performed a comprehensive meta-analysis to precisely estimate of the impact of the hOGG1 Ser326Cys polymorphism on gynecologic cancer susceptibility. Electronic databases including PubMed, Embase, WanFang, and the China National Knowledge Infrastructure were searched for relevant studies. Odds ratios (ORs) with corresponding 95% confidence intervals (CIs) were determined to assess the strength of the association. Fourteen studies with 2712 cases and 3638 controls were included in the final meta-analysis. The pooled analysis yielded a significant association between the hOGG1 Ser326Cys polymorphism and overall gynecologic cancer susceptibility (dominant model: OR = 1.16, 95% CI = 1.03–1.30, P=0.017). A significantly higher gynecologic cancer risk was found for the European population (homozygous model: OR = 2.17, 95% CI = 1.80–2.61, P<0.001; recessive model: OR = 2.11, 95% CI = 1.41–3.17, P<0.001; dominant model: OR = 1.29, 95% CI = 1.12–1.48, P<0.001; and allele model: OR = 1.40, 95% CI = 1.13–1.74, P=0.002), but not in the Asian population. The stratified analysis by cancer type revealed endometrial cancer was significantly associated with the hOGG1 Ser326Cys polymorphism (dominant model: OR = 1.29, 95% CI = 1.09–1.54, P=0.003; and allele model: OR = 1.28, 95% CI = 1.02–1.60, P=0.031). In conclusion, the hOGG1 Ser326Cys polymorphism was associated with higher overall gynecologic cancer susceptibility, especially for endometrial cancer in the European population.

Association study between polymorphisms of XRCC1, hOGG1 in DNA repair gene and the risk of endometrial carcinoma: a meta-analysis

Objective The association of DNA repair gene XRCC1, hOGG1 polymorphisms with susceptibility to endometrial carcinoma (EC) have been extensively studied with inconsistent results. The objective of this study was to clarify this issue by a comprehensive review and meta-analysis. Methods English (PubMed, Medline, Embase) and Chinese (CNKI, wanfang) electric databases were searched to collect a case-control study on the association between XRCC1 or hOGG1 gene polymorphisms and endometrial carcinoma. The retrieval time was from the database until may 1th, 2019. According to inclusion and exclusion criteria, relevant data of the final included literature were extracted and STATA 11.0 software was used for meta analysis. Results A total of 8 references meeting the inclusion criteria were included for analysis from the 243 retrieved literatures. The Arg399Gln polymorphism at the XRCC1 gene was associated with increased susceptibility to endometrial carcinoma (OR=1.36, 95%CI=1.23~1.51, P<0.001) in the overall population, the same results were shown in the subgroup analysis of Caucasians (OR=1.44, 95%CI=1.29~1.61, P<0.001). Ser326Cys polymorphism of the hOGG1 gene also increases the risk of endometrial carcinoma in Caucasians (OR=1.54, 95%CI=1.34~1.76, P=0.001). No publication bias was detected in this meta-analysis. Conclusions This meta-analysis provided evidence that the Arg399Gln polymorphism of DNA repair gene XRCC1 may increase risk of endometrial carcinoma, especially in the Caucasian. Moreover, Ser326Cys polymorphism of hOGG1 increase endometrial carcinoma risk in the Caucasian.

Impact of the Ser326Cys polymorphism of the OGG1 gene on the level of oxidative DNA damage in patients with colorectal cancer

As a result of reactive oxygen species operation, cell damage occurs in both cellular organelles and molecules, including DNA. Oxidative damage within the genetic material can lead to accumulation of mutations and consequently to cancer transformation. OGG1 glycosylase, a component of the Base Excision Repair (BER) system, is one of the enzymes that prevents excessive accumulation of 8-oxoguanine (8-oxG), the most common compound formed by oxidative DNA damage. In case of structural changes of OGG1 resulting from polymorphic variants, we can observe a significant increase in the concentration of 8-oxG. Linking individual polymorphisms to DNA repair systems with increased risk of colorectal cancer will allow patients to be classified as high risk and included in a prophylactic program. The aim of the study was to determine the level of oxidative DNA damage and to analyze the distribution of Ser326Cys polymorphism of the OGG1 gene in a group of patients with colorectal cancer and in a control group in the Polish population. Material and methodology. DNA was isolated from the blood of 174 patients with colorectal cancer. The control group consisted of 176 healthy individuals. The level of oxidative damage was determined by analyzing the amount of 8-oxguanine using the HT 8-oxo-dG ELISA II Kit. Genotyping was performed via the TaqMan method. Results. The obtained results indicate that Ser326Cys polymorphism of the OGG1 gene increases the risk of RJG and is associated with significantly increased levels of 8-oxoguanine. Conclusions. Based on the results obtained, we conclude that Ser326Cys polymorphism of the OGG1 gene may modulate the risk of colorectal cancer by increasing the level of oxidative DNA damage.