scholarly journals Applying PICRUSt and 16S rRNA functional characterisation to predicting co-digestion strategies of various animal manures for biogas production

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Grace N. Ijoma ◽  
Rosina Nkuna ◽  
Asheal Mutungwazi ◽  
Charles Rashama ◽  
Tonderayi S. Matambo
Keyword(s):  
Biogas Production ◽  
Integrated Approach ◽  
Animal Manure ◽  
Nucleotide Sugar ◽  
Linear Discriminant ◽  
Biogas Yield ◽  
Functional Characterisation ◽  
Considerable Impact ◽  
Substrate Choice ◽  
Waste Degradation

AbstractAn estimated 25 million tons of animal manure is produced globally every year, causing considerable impact to the environment. These impacts can be managed through the use of anaerobic digestion (AD) This process achieves waste degradation through enzymatic activity, the efficiency of the AD process is directly related to microorganisms that produce these enzymes. Biomethane potential (BMP) assays remain the standard theoretical framework to pre-determine biogas yield and have been used to determine the feasibility of substrates or their combination for biogas production. However, an integrated approach that combines substrate choice and co-digestion would provide an improvement to the current predictive models. PICRUSt (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States) addresses the limitations of assays in this regard. In this paper, the biochemical functions of horse, cow, and pig manures are predicted. A total of 135 predicted KEGG Orthologies (KOs) showed amino acids, carbohydrate, energy, lipid, and xenobiotic metabolisms in all the samples. Linear discriminant analysis (LDA) combined with the effect size measurements (LEfSe), showed that fructose, mannose, amino acid and nucleotide sugar, phosphotransferase (PST) as well as starch and sucrose metabolisms were significantly higher in horse manure samples. 36 of the KOs were related to the acidogenesis and/or acetogenesis AD stages. Extended bar plots showed that 11 significant predictions were observed for horse-cow, while 5 were predicted for horse-pig and for cow-pig manures. Based on these predictions, the AD process can be enhanced through co-digestion strategies that takes into account the predicted metabolic contributions of the manure samples. The results supported the BMP calculations for the samples in this study. Biogas yields can be improved if this combined approach is employed in routine analysis before co-digesting different substrates.

scholarly journals Sustainable Use Of Macro-Algae For Biogas Production In Latvian Conditions: A Preliminary Study Through An Integrated Mca And Lca Approach

2014 ◽  
Vol 13 (1) ◽  
pp. 44-56 ◽  
Author(s):  
Laura Pastare ◽  
Francesco Romagnoli ◽  
Dace Lauka ◽  
Ilze Dzene ◽  
Tatjana Kuznecova
Keyword(s):  
Biogas Production ◽  
Sustainable Use ◽  
Integrated Approach ◽  
Energy System ◽  
Algae Biomass ◽  
Biogas Yield ◽  
Beneficial Effects ◽  
Biogas Energy ◽  
Preliminary Study ◽  
Natural Water Bodies

Abstract The study focuses on sustainability evaluation of an algae-based energy system in Latvia with a holistic and integrated approach of multi-criteria analysis combined with life cycle assessment (including a practical side - biogas yield experiments of locally available algae). The study shows potential for sustainable use of algae in Latvian conditions and thus that algal biomass can be utilized for the production of biogas. The most sustainable and feasible scenario of using algae for biogas energy production foresees the collection of algae biomass from natural water bodies. Important beneficial effects through the use of algae are related to avoiding global warming potential (GWP) and eutrophication impacts. Biogas batch experiments carried out with the local macrophyte C.demersum have shown a methane yield of 554 l CH4/kg VS.

Study on Biogas Production by Dry Anaerobic Co-Digestion of Animal Manure and Straw

2011 ◽  
Vol 236-238 ◽  
pp. 98-103
Author(s):  
Li Jun Shi ◽  
Li Tong Ban ◽  
Hui Fen Liu ◽  
Jian Chao Hao ◽  
Wei Yu Zhang
Keyword(s):  
Biogas Production ◽  
Animal Manure ◽  
Chicken Manure ◽  
Pig Manure ◽  
Cow Manure ◽  
Apparent Effect ◽  
Biogas Yield ◽  
Operation Temperature ◽  
Start Up ◽  
Dry Digestion

Dry anaerobic co-digestion of animal manure and straw was conducted to produce biogas. Startup characteristics and biogas production perform of dry digestion were studied, and the effect of operation temperature and incubation amount on dry digestion was also investigated. The study result showed that under the conditions of C/N=25-30, TS=20% and T=(36±1) °C,dry digestion can start up quickly with acclimated thickening sludge as incubation sludge. Compared to dry digestion of chicken manure and pig manure, dry digestion of cow manure proceeded steadily with high biogas yield. It is found that incubation is necessary in the process of dry digestion and biogas yield increases with more incubation amount. The appreciate incubation ratio is about 10%. Temperature change has apparent effect on biogas production, and it is suggested that mesophilic temperature should be chosen in the scaled project of dry digestion.

scholarly journals Evaluating the Potential of Domestic Animal Manure for Biogas Production in Ethiopia

2020 ◽  
Vol 2020 ◽  
pp. 1-4
Author(s):  
Fikadu Kumsa Gemechu
Keyword(s):  
Fossil Fuels ◽  
Biogas Production ◽  
Animal Manure ◽  
Domestic Animal ◽  
Cow Dung ◽  
Renewable Energy Resources ◽  
Biogas Yield ◽  
Sheep Manure ◽  
World Demand ◽  
Materials Used

Energy is one of the most important needy resources that found in the form of renewable and nonrenewable sources. The world demand for energy grows rapidly, and therefore, it is a time to look alternative and renewable energy resources to replace the rapidly depleting supply of fossil fuels. This study is aimed at analyzing the effects of temperature (°C), retention time (days), and potential of animal waste on the biogas production and its %CH4 compositions as responses to the factors of the study. The materials used in this study were cow dung, sheep, and pig manures. Anaerobic batch digesters (plastic water bottle) with a total volume of 2000 ml were used as digester (bioreactor) in this experiment. The glucose drip (tube) was fitted to the lids of each digester. Average temperature of digester was increased starting from the 6th to 10th day in cow dung and sheep manure. In this experiment, optimum time for best biogas yield was recorded. Analysis of the gas component shows the significant volume of methane component recorded in cow dung (66.9%) followed by sheep manure (62.1%). Cow dung was one of the best in producing biogas, while the sheep manure was medium, and pig manures are fewer producers as compared to others.

scholarly journals Effect of Organic Waste Addition into Animal Manure on Biogas Production Using Anaerobic Digestion Method

2021 ◽  
Vol 10 (3) ◽  
pp. 623-633
Author(s):  
Fahmi Arifan ◽  
Abdullah Abdullah ◽  
Siswo Sumardiono
Keyword(s):  
Anaerobic Digestion ◽  
Fossil Fuels ◽  
Organic Waste ◽  
Biogas Production ◽  
Liquid Waste ◽  
Animal Manure ◽  
Raw Material ◽  
Chicken Manure ◽  
Cow Dung ◽  
Biogas Yield

One biomass form with a high potential to replace fossil fuels is biogas. Biogas yield production depends on the raw material or substrate used. This research was aimed to investigate abiogas production technique using an anaerobic digestion process based on a substrate mixture of a starter, cow dung, chicken manure, tofu liquid waste, and cabbage waste.The anaerobic digestion is a promised process to reduce waste while it is also producing renewable energy.Moreover, the process can digest high nutrients in the waste. The anaerobic digestion results showed that the combination producing the highest biogas amount was 200 mg starter mixed with a ratio of 70% cow dung, 15% chicken manure, and 15% tofu liquid waste. The larger the amount of cabbage waste, the lower the biogas production. The quadratic regression analysisand kinetics model based on the Gompertz equation was obtained for the variable with the highest yield, compared to 70% cow dung, 15% chicken manure, and 15% tofu liquid waste and the estimated kinetic parameters based on the Gompertz equations revealed that the value of P∞ = 2,795.142 mL/gr.Ts, Rm = 113, 983.777 mL/gr.Ts, and t = 10.2 days. The results also conluded that the use of  tofu liquid waste produced more biogas than cabbage waste. This study also successfully showed significant development in terms of the amount of biogas produced by adding organic waste to animal manure as the substrate used

Optimization of Operation Parameters of Dry Anaerobic Co-Digestion from Animal Manure and Straw in a Batch Reactor

2014 ◽  
Vol 884-885 ◽  
pp. 475-480
Author(s):  
Li Jun Shi ◽  
Wen Lan Liu ◽  
Hui Fen Liu ◽  
Wei Yu Zhang
Keyword(s):  
Biogas Production ◽  
Batch Reactor ◽  
Animal Manure ◽  
Chicken Manure ◽  
Pig Manure ◽  
Apparent Effect ◽  
Biogas Yield ◽  
Operation Temperature ◽  
Start Up ◽  
Dry Digestion

Dry anaerobic co-digestion of animal manure and straw was conducted to produce biogas. Startup characteristics and biogas production perform of dry digestion were studied, and the effect of operation temperature and incubation amount on dry digestion was also investigated. The study result showed that under the conditions of C/N=25-30, TS=20% and T=(36±1)°C,dry digestion can start up quickly with acclimated thickening sludge as incubation sludge. Compared to dry digestion of chicken manure and pig manure, dry digestion of cow manure proceeded steadily with high biogas yield. It is found that incubation is necessary in the process of dry digestion and biogas yield increases with more incubation amount. The appreciate incubation ratio is about 10%. Temperature change has apparent effect on biogas production, and it is suggested that mesophilic temperature should be chosen in the scaled project of dry digestion.

scholarly journals The Potential of the Biodigester as a Useful Tool in Coffee Farms

2021 ◽  
Vol 11 (15) ◽  
pp. 6884
Author(s):  
Mauricio Hernández-Sarabia ◽  
Juan Sierra-Silva ◽  
Liliana Delgadillo-Mirquez ◽  
Julián Ávila-Navarro ◽  
Laura Carranza
Keyword(s):  
Fermentation Process ◽  
Biogas Production ◽  
Animal Manure ◽  
Agricultural Product ◽  
Coffee Production ◽  
Coffee Waste ◽  
Coffee Cultivation ◽  
Harmful Effects ◽  
Wet Fermentation ◽  
Potential Tool

Coffee is a highly productive agricultural product in the department of Tolima. The wet fermentation process of coffee generates about 80% of the waste, which is highly contaminated with organic matter that pollutes water sources, so anaerobic co-digestion techniques are implemented in coffee wastewater using Taiwan type biodigesters. According to the study of three biodigesters fed with coffee waste and animal manure, the aim is to show their potential application in coffee farms. These biodigesters generated a biogas production with CH4 concentrations between 49.1% ± 4.6 and 58.1% ± 2.4 in volume, in addition to the benefit of the biol used as fertilizer in the crops. The biogas produced can be used for cooking and can save around USD 40.17 in natural gas. Therefore, the biodigester is a potential tool to mitigate the harmful effects of coffee cultivation on the surrounding ecosystem and can project coffee production in a sustainable direction.

scholarly journals Biogas potential of organic waste onboard cruise ships — a yet untapped energy source

2021 ◽  
Author(s):  
Kai Schumüller ◽  
Dirk Weichgrebe ◽  
Stephan Köster
Keyword(s):  
Energy Source ◽  
Anaerobic Digestion ◽  
Sewage Sludge ◽  
Food Waste ◽  
Energy Demand ◽  
Organic Waste ◽  
Biogas Production ◽  
Biogas Yield ◽  
Cruise Ships ◽  
Detailed Presentation

AbstractTo tap the organic waste generated onboard cruise ships is a very promising approach to reduce their adverse impact on the maritime environment. Biogas produced by means of onboard anaerobic digestion offers a complementary energy source for ships’ operation. This report comprises a detailed presentation of the results gained from comprehensive investigations on the gas yield from onboard substrates such as food waste, sewage sludge and screening solids. Each person onboard generates a total average of about 9 kg of organic waste per day. The performed analyses of substrates and anaerobic digestion tests revealed an accumulated methane yield of around 159 L per person per day. The anaerobic co-digestion of sewage sludge and food waste (50:50 VS) emerged as particularly effective and led to an increased biogas yield by 24%, compared to the mono-fermentation. In the best case, onboard biogas production can provide an energetic output of 82 W/P, on average covering 3.3 to 4.1% of the total energy demand of a cruise ship.

The Influence of Leachate Refluence on Dry Mesophilic Co-Fermentation

2013 ◽  
Vol 295-298 ◽  
pp. 1735-1739
Author(s):  
Fu Bin Yin ◽  
Zi Fu Li ◽  
Shuang Hou ◽  
Xiao Feng Bai ◽  
Ting Ting Wang
Keyword(s):  
Biogas Production ◽  
Methane Content ◽  
Chicken Manure ◽  
Good Effect ◽  
Corn Straw ◽  
Biogas Yield

The main objectives of this research were to determine the effect of leachate refluence on biogas production for dry mesophilic co-fermentation of chicken manure and corn straw. The biogas production, the ratio of biogas production, methane content and pH were analyzed. The results showed that the leachate refluence has a significant impact on biogas production of dry co-fermentation. The cumulative biogas yield of the once in 48h has an increase by 10% and 5% for no reflux and once in 24h, respectively. The leachate refluence has little influence on the methane content, but it has good effect to keep pH in the optimum rang.

Innovative use of dissolved air flotation with biosorption as primary treatment to approach energy neutrality in WWTPs

2015 ◽  
Vol 10 (1) ◽  
pp. 133-142 ◽  
Author(s):  
H.-B. Ding ◽  
M. Doyle ◽  
A. Erdogan ◽  
R. Wikramanayake ◽  
P. Gallagher
Keyword(s):  
Biogas Production ◽  
Primary Treatment ◽  
Anaerobic Sludge ◽  
Excess Sludge ◽  
Dissolved Air Flotation ◽  
Biogas Yield ◽  
Air Flotation ◽  
Sludge Thickening ◽  
Plant Capacity ◽  
Dissolved Air

This paper presents two types of dissolved air flotation application together with biosorption (the ‘Captivator® system’) as primary treatments. In the first instance, the Captivator® system is the sole primary treatment for a new plant installation and helps to gain 65% more biogas while requiring only 44% of aeration for COD oxidation, compared to a conventional process with a primary clarifier. In the second application, the Captivator® system is used to enhance the existing primary treatment for plant capacity expansion. With digested anaerobic sludge recycled as an additional adsorbent, the Captivator® system in the second application increases the biogas yield by 52% and only generates 59% excess sludge. Overall, the Captivator® system would help WWTPs to approach energy neutrality by diverting more organics for biogas production and reducing the energy requirements for aeration. In addition, it would help to reduce the installation footprint for primary treatment and save considerable capital cost by eliminating the sludge thickening process.

scholarly journals Effect of Hydraulic Retention Time on Biogas Production from Cow Dung in A Semi Continuous Anaerobic Digester

2018 ◽  
Vol 7 (2) ◽  
pp. 93-100 ◽  
Author(s):  
Agus Haryanto ◽  
Sugeng Triyono ◽  
Nugroho Hargo Wicaksono
Keyword(s):  
Retention Time ◽  
Hydraulic Retention Time ◽  
Loading Rate ◽  
Biogas Production ◽  
Stable Condition ◽  
Anaerobic Digester ◽  
Organic Loading Rate ◽  
Cow Dung ◽  
Biogas Yield ◽  
Average Analysis

The efficiency of biogas production in semi-continuous anaerobic digester is influenced by several factors, among other is loading rate. This research aimed at determining the effect of hydraulic retention time (HRT) on the biogas yield. Experiment was conducted using lab scale self-designed anaerobic digester of 36-L capacity with substrate of a mixture of fresh cow dung and water at a ratio of 1:1. Experiment was run with substrate initial amount of 25 L and five treatment variations of HRT, namely 1.31 gVS/L/d (P1), 2.47 gVS/L/d (P2), 3.82 gVS/L/d (P3), 5.35 gVS/L/d (P4) and 6.67 gVS/L/d (P5). Digester performance including pH, temperature, and biogas yield was measured every day. After stable condition was achieved, biogas composition was analyzed using a gas chromatograph. A 10-day moving average analysis of biogas production was performed to compare biogas yield of each treatment. Results showed that digesters run quite well with average pH of 6.8-7.0 and average daily temperature 28.7-29.1. The best biogas productivity (77.32 L/kg VSremoval) was found in P1 treatment (organic loading rate of 1.31 g/L/d) with biogas yield of 7.23 L/d. With methane content of 57.23% treatment P1 also produce the highest methane yield. Biogas production showed a stable rate after the day of 44. Modified Gompertz kinetic equation is suitable to model daily biogas yield as a function of digestion time.Article History: Received March 24th 2018; Received in revised form June 2nd 2018; Accepted June 16th 2018; Available onlineHow to Cite This Article: Haryanto, A., Triyono, S., and Wicaksono, N.H. (2018) Effect of Loading Rate on Biogas Production from Cow Dung in A Semi Continuous Anaerobic Digester. Int. Journal of Renewable Energy Development, 7(2), 93-100.https://doi.org/10.14710/ijred.7.2.93-100

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