Abstract
Objective To prepare borneol angelica polysaccharide liposomes (BAPLs) with distinct characteristics in Chinese and Western medicine, to explore their effects on anti-cerebral ischemia-reperfusion inflammatory reactions and to explain their mechanism. Methods Under the guidance of the basic theory of traditional Chinese medicine, a new dosage form of nanoliposomes was selected to prepare BAPL by the thin film dispersion ultrasonic method. The effects of angelica polysaccharide (AP), lecithin, cholesterol and ultrasonication time on encapsulation efficiency were investigated. Triphenyltetrazolium chloride (TTC) staining was used to detect the volume of cerebral infarction. Western blotting was used to detect the expression levels of TLR-4, NF-κBp65, ZO-1, ZO-2, IL-1β, IL-6, IL-8 and IL-10. Results As the mass ratio of lecithin to cholesterol (X1), the mass of angelica polysaccharide (X2) and the interaction between X1 and X2 (X1X2) were used as investigation factors, the fitting equation between encapsulation efficiency Y and X was Y=0.307-0.153X1+0.026 X2+0.006X12-0.000301 X22+0.002X1X2 (P<0.05). The best ultrasonication time was 15 min, the highest encapsulation efficiency was 80.4%, the particle size was 179.1 nm, and the surface zeta potential was -17.2 mV. In BAPL group, the infarct volume of TTC staining was significantly decreased (P<0.05), and the expression levels of NF-κBp65, TLR-4, IL-8, IL-6, IL-1β in brain tissue were significantly decreased (P<0.05), while the expression levels of ZO-1, ZO-2, IL-10 were significantly increased (P<0.05). Conclusion BAPL has high encapsulation efficiency, small particle size and stable properties and can alleviate cerebral ischemia-reperfusion injury. Its mechanism may be related to inhibiting inflammatory reactions, downregulating inflammatory reaction signaling pathways and protecting the blood–brain barrier (BBB).