Identification and Characterization of Fungi Causing Thread Blight Diseases on Cacao in Ghana

Theobroma cacao (chocolate tree) is currently under serious threat from thread blight disease (TBD), which has been attributed to the causal agent Marasmiellus scandens in other regions of the world. TBD in Ghana has similar symptomology but variable signs. This study sought to determine whether TBD in Ghana was caused by a single agent and whether Marasmiellus scandens was a significant agent of TBD. Forty-eight isolates were collected from eight geographical locations in Ghana for morphological and molecular characterization. Disease signs occurred as vegetative rhizomorphs or hyphal aggregates, which were classified into five morphotypes: A, abundant thin, black, “horse hair”-type rhizomorphs; B, scattered brown rhizomorphs; C, whitish to brownish-white; D, faint cream or dull white; and E, aggregates of shiny or silky white hyphae. Sequencing and analyses of three loci—the internal transcribed spacer region of the nuclear ribosomal repeat, nuclear large subunit, and mitochondrial small subunit—detected four species, all members of the Marasmiaceae, causing TBD-like disease. These were identified as Marasmius crinis-equi (morphotype A), Marasmius tenuissimus (morphotypes B and C), Marasmiellus palmivorus (morphotype E), and Marasmiellus scandens (morphotype D). Marasmius tenuissimus, the most frequently isolated TBD fungus in this study, is primarily an Asian fungus and not previously associated with diseases of cacao. Marasmiellus palmivorus, the second most frequently isolated fungus, is a pan-tropical pathogen with a broad host range; this is the first report of the fungus causing TBD on cacao. Marasmius crinis-equi also has a broad pan-tropical distribution and host range and causes thread blight on several tropical tree crops. Surprisingly, Marasmiellus scandens, the most frequently cited agent of TBD in cacao, made up only 8% of the isolates.

Hair follicle regional specificity in different parts of bay Mongolian horse by histology and transcriptional profiling

Background Different morphological structures of hairs having properties like defense and camouflage help animals survive in the wild environment. Horse is one of the rare kinds of animals with complex hair phenotypes in one individual; however, knowledge of horse hair follicle is limited in literature and their molecular basis remains unclear. Therefore, the investigation of horse hair follicle morphogenesis and pigmentogenesis attracts considerable interest. Result Histological studies revealed the morphology and pigment synthesis of hair follicles are different in between four different parts (mane, dorsal part, tail, and fetlock) of the bay Mongolian horse. Hair follicle size, density, and cycle are strongly associated with the activity of alkaline phosphatase (ALP). We observed a great difference in gene expression between the mane, tail, and fetlock, which had a greater different gene expression pattern compared with the dorsal part through transcriptomics. The development of the hair follicle in all four parts was related to angiogenesis, stem cells, Wnt, and IGF signaling pathways. Pigmentogenesis-related pathways were involved in their hair follicle pigment synthesis. Conclusions Hair follicle morphology and the activity of ALP differ among four body parts in bay Mongolian horse. Hair follicles of the different body parts of the are not synchronized in their cycle stages. GO terms show a regional specificity pattern between different skin parts of the bay Mongolian horse. These results provide an insight into the understanding of the biological mechanism of the hair follicle in other mammals.

Keep Your (Horse) Hair On? Experimental Evidence on the Effect of Exposure to Legitimising Symbols on Diffuse Support for the High Court

Positivity theory posits that the courts rely on powerful legitimising symbols—such as elaborate judicial attire, honorific forms of address and imposing courtroom design—to ensure legitimacy in the eyes of the public in the absence of an electoral mandate. The argument is that such legitimising symbols evoke images of learning and pageantry and create the presumption that the process by which the decision was made was fair. Typically, positivity theory has been tested by examining whether people who have a greater awareness or knowledge of the courts express higher diffuse support for their decisions. Yet, such an approach assumes that those who know more about the courts will have greater exposure to their legitimising symbols. It does not directly test if exposure to the courts’ legitimising symbols causes people to be more acquiescent with decisions with which they disagree. In this article we use a survey-based experiment to examine if exposure to the legitimising symbols of the High Court makes people more willing to accept decisions of the Court with which they disagree. We assess whether the decision of the High Court Justices to simplify their attire, including, since 1988, ceasing to wear wigs when sitting on the Bench, has adversely affected the Court’s institutional legitimacy by removing some of the mystique associated with the decision-making process. We find that exposure to the Court’s legitimising symbols is associated with higher acquiescence with decisions which people disagree with, but the Court’s decision to simplify the Justices’ attire has not adversely affected diffuse support for its decisions. Our findings are important because the Court is reliant on maintaining legitimacy to enforce the rule of law. Our results speak directly to how the Court can best take steps to increase its institutional legitimacy in the eyes of the public.

Creating artificial Rhino Horns from Horse Hair

Demand for rhino horn is driving poaching with devastating effect for the few individuals left of the few species surviving from this once numerous, widespread and cosmopolitan clade of pachyderms. We bundled together tail hairs of the rhino’s ubiquitous near relative, the horse, to be glued together with a bespoke matrix of regenerated silk mimicking the collagenous component of the real horn. This approach allowed us to fabricate composite structures that were confusingly similar to real rhino horn in look, feel and properties. Spectral and thermal FT-IR, DSC and TGA analysis demonstrated the similar chemical composition and thermo-mechanical properties between the natural and the faux horns.

Study on principal component analysis and multi-class discrimination against the combination to identify easy-confused fur

In this paper, the principal component analysis was performed on infrared spectral data, which included 60 groups of cattle and horse hair surfaces and 60 groups of cattle and horse flesh surfaces, respectively, by using SPSS 22. Using 100 sets of data as modelling samples (50 sets of cattle fur and horse fur), the multi-class discriminant analysis was carried out by using SPSS, the typical discriminant function and class function of cattle fur and horse fur were established and the back substitution verification was carried out. The typical discriminant function and the class function of cattle fur and horse fur were verified by 20 sets of data validation samples (10 groups of cattle fur and horse fur). The results show that the principal component analysis can reduce the dimension effectively, reduce the hair surfaces spectra from 2696 wavelength variables to nine wavelength variables and decrease the flesh surfaces spectra from 2696 wavelength variables to 13 wavelength variables. The cumulative contribution rate of the new wavelength variables is up to 99.89 and 99.88%, respectively. The back substitution accuracy rate of the typical discriminant function is 100%, and the verification accuracy rate is 100%. From the clustering graph, the established class function of cattle fur and horse fur back substitution clustering is well, and the correct clustering rate of the verification clustering graph is 100%.