Research on Separation and Purification of Total Anthraquinones in Radix et Rhizoma Rhei by D301 Macroporous Resin

2015 ◽  
Vol 1092-1093 ◽  
pp. 630-634
Author(s):  
Cui Ping Li ◽  
Ping Lv
Keyword(s):  
Hydrochloric Acid ◽  
Flow Velocity ◽  
Extraction Efficiency ◽  
Strong Stability ◽  
Adsorption Rate ◽  
Dynamic Adsorption ◽  
Desorption Rate ◽  
Separation And Purification ◽  
Macroporous Resin ◽  
Radix Et Rhizoma Rhei

D301 resin was used to separate and purify total anthraquinones in Radix et Rhizoma Rhei. It was found that the optimized dynamic adsorption conditions is pH7.0, flow velocity of 1.0 BV/h and extracting solution with concentration of 0.3g/mL and optimized dynamic elution conditions is hydrochloric acid with concentration of 0.15mol/L, 75% ethanol, and flow velocity of 1.0 BV/h. Under the above conditions,the average adsorption rate and desorption rate are respectively 89.75% and 91.15%, indicating that extraction efficiency of total anthracenone of emodin is relatively high and that the technology enjoys strong stability.

Study on Macroporous Resin Separation and Purification of Total Flavonoids of Plantago Process

2012 ◽  
Vol 550-553 ◽  
pp. 987-992 ◽  
Author(s):  
Yong Guang Bi ◽  
Yao Quan Tan
Keyword(s):  
Volume Fraction ◽  
Stable Process ◽  
Process Conditions ◽  
Total Flavonoids ◽  
Separation And Purification ◽  
Optimum Conditions ◽  
Flavonoid Content ◽  
Macroporous Resin ◽  
Crude Drug ◽  
Resin Separation

To study the macroporous resin separation and purification of total flavonoids of Plantago optimum conditions, the static adsorption and desorption experiments by filtering out the best macroporous resin, and plantain extract for the assessment of total flavonoid content indicators in the single-factor test based on the orthogonal design, the amount of resin in the hole, eluent concentration, alcohol consumption and other factors for the study to optimize the AB-8 macroporous resin separation and purification of total flavonoids plantain best process conditions. The results show that: the effect of various factors affecting the size of the order of separation and purification has to: eluent concentration> ethanol consumption> macroporous resin usage, optimization of the optimum conditions: resin dosage is 1 times the amount of crude drug, the use of eluent volume fraction of 40% ethanol, elution agent is 12.5 times the amount of crude drug. Verify the conditions in this test, three repeated experiments were similar, the average flavonoid content 0.2127%, the transfer rate of 90.57%, indicating that the scientific and rational and stable process conditions can be effectively used plantain purified flavonoids.

scholarly journals Preparation of Hydroxytyrosol by Acid Hydrolysis from Olive Leaves

Separations ◽  
2021 ◽  
Vol 8 (9) ◽  
pp. 159
Author(s):  
Yujie Wang ◽  
Siyuan Luo ◽  
Jieling Luo ◽  
Jipeng Qu ◽  
Shiling Feng ◽  
...  
Keyword(s):  
Hydrochloric Acid ◽  
Flow Rate ◽  
Bioactive Compound ◽  
Extraction Process ◽  
Extraction Methods ◽  
Hydrochloric Acid Concentration ◽  
Macroporous Resin ◽  
Olive Leaves ◽  
Ultrasonic Time ◽  
Optimal Extraction Condition

Hydroxytyrosol (HT) is the main bioactive compound in olive leaves. However, olive leaves contain a lower level of HT and the extraction process of HT was rarely optimized. In this study, compared with two extraction methods, ultrasound was found to have a positive effect on improving the yield of HT. Therefore, ultrasound was used to assist hydrolysis of hydrochloric acid to extract HT from olive leaves. Response surface method and macroporous resins were applied to optimize the extraction process as well as enrichment of HT. The results showed that ultrasonic extraction time had a significant effect on the yield and the optimal extraction conditions were obtained: ultrasonic time was 120 min, hydrochloric acid concentration was 1.60 mol/L and the liquid-to-material ratio was 60.00 mL/g. Under the optimal extraction condition, the yield of HT was 14.11 ± 0.12 mg/g. NKA-Ⅱ macroporous resin was proved to be a suitable resin to enrich HT from extraction solution. The optimal condition for enriching HY was 250 mL of loading solution at the flow rate of 1.5 mL/min with 40 mL volume of 75% ethanol–eluent at a flow rate of 1.0 mL/min. The concentration of HT changed from 2.27% to 9.25% after enrichment by macroporous resin.

Separation and purification of l-methionine from E. coli fermentation broth by macroporous resin chromatography

2019 ◽  
Vol 1110-1111 ◽  
pp. 108-115 ◽  
Author(s):  
Neng Xiong ◽  
Ru Yu ◽  
Tao Chen ◽  
Ya-Ping Xue ◽  
Zhi-Qiang Liu ◽  
...  
Keyword(s):  
Fermentation Broth ◽  
Separation And Purification ◽  
Macroporous Resin ◽  
E Coli

The Study of Purifying Coumarin Chemical Compounds in “Yubaizhi” Angelica Dahurica by Macro-Porous Absorption Resin

2012 ◽  
Vol 468-471 ◽  
pp. 3060-3064
Author(s):  
Yan Long Wang ◽  
Hao Guo Tang ◽  
Mi Mi Guo
Keyword(s):  
Chemical Compounds ◽  
Adsorption Rate ◽  
Angelica Dahurica ◽  
Desorption Rate ◽  
Sample Material ◽  
Sample Concentration

The method of using macro-porous absorption resin to rurify coumarins in “Yubaizhi” angelica dahurica was used in this study. A kind of better resin was chosen from D-101 resin and HPD600 resin by the comparison of adsorption rate, desorption rate and purity. The result showed that D-101 resin was better for purifying coumrins. Then these conditions of purifying were discussed. when the sample concentration was 0.1g medicinal materials per milliliter and the adsorbent speed was 1ml/min, the effect of adsorption was better. And per gram resin could adsorb coumarins of 3g sample material. When the eluotropic speed of 80% ethanol was 1ml/min and the dosage of eluent reaches was 7 multiple of bed volume, the effect of elution was better. The result of demonstration test showed the effect of purifying coumarin was better with above-mentioned conditions.

EFFECT OF SOLVENT THE SELECTIVE SEPARATION OF PHYTOSTEROLS USING ZEOLITE

REAKTOR ◽  
2006 ◽  
Vol 10 (1) ◽  
pp. 31
Author(s):  
A.Widjaja A.Widjaja ◽  
Chuang Y.H. Chuang
Keyword(s):  
Organic Solvent ◽  
Boiling Point ◽  
Selective Separation ◽  
Adsorption Rate ◽  
Desorption Rate ◽  
Zeolite Surface ◽  
Solvent Selectivity ◽  
Effect Of Solvent ◽  
High Volatility ◽  
High Boiling Point

The effect of organic solvent on zeolite adsorption of sterols mixiture containing almost the same percentage of campesterol and B-sitosterol was investigated. Although pentane was found to give the highest adsorption rate than other solvents, but it was not chosen as the sovent due to its low boilingpoint and high volatility. Although iso-actane has a high boiling point and adsorption rate like pentane, however, since it shows lower  selectivity than other solvents, it was not considered as a good solvent. Based on the the selectivity study which shows that using hexane as the solvent campesterol was adsorbed more than B-sitosterol, and also based on the moderate boiling point of hexane, hexane wasconsidered the best solvent for the separation of campesterol and B-sitosterol by zeolite adsorption. It was found that adding up to 0.3% ethanol could increase the adsorption rate. Methanol, having the highest polarity among other solvents investigated, gave the highest desorption rate of campesterol adsorbed  on the zeolite surface. However, solvent selectivity in the desorption was lower than that in  adsorption.

scholarly journals Technologies for the Extraction, Separation and Purification of polyphenols – A Review

2019 ◽  
Vol 6 (1) ◽  
pp. 74-91 ◽  
Author(s):  
Shyam Suwal ◽  
Alice Marciniak
Keyword(s):  
Organic Molecules ◽  
Extraction Efficiency ◽  
Positive Impact ◽  
Cost Effective ◽  
Separation And Purification ◽  
Advantages And Disadvantages ◽  
Assisted Extraction ◽  
Extraction Separation ◽  
Current Context ◽  
Conventional Extraction

Polyphenols are high molecular weight, organic molecules mainly found in plant kingdom. They are mostly known for their positive impact on health, specifically for their antioxidant activity. Indeed, they are widely studied for the prevention of multiple diseases such as cancer, inflammatory, cardiovascular and neurodegenerative diseases. Nevertheless, extractions of these growing interest molecules remain challenging using conventional methods such as solvent extraction. That is why recent researches have focused on improving the extraction of polyphenol by using different technologies such as ultrasound, microwave, pressurized liquid, pulsed electric field, supercritical fluid and high hydrostatic pressure. In the current context, the assisted-extraction should demonstrate their potential to improve the extraction efficiency while being cost-effective and with a low environmental impact. To this end, technologies ought to, for instance, increase the solubility of polyphenol and the permeability of the cell wall. Consequently, this review is focused on the use and potential of these technologies to improve polyphenol extractions from plants as well as their purification using various methods. It discusses of the advantages and disadvantages with some examples of all these technologies assisted-extraction in comparison with conventional extraction method as well as purification technology.

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