Sequence assignment validation in cryo-EM models with checkMySequence

The availability of new AI-based protein structure prediction tools radically changed the way cryo-EM maps are interpreted, but it has not eliminated the challenges of map interpretation faced by a microscopist. Models will continue to be locally rebuilt and refined using interactive tools. This inevitably results in occasional errors, among which register-shifts remain one of the most difficult to identify and correct. Here we introduce checkMySequence; a fast, fully automated and parameter-free method for detecting register-shifts in protein models built into cryo-EM maps. We show that the method can assist model building in cases where poorer map resolution hinders visual interpretation. We also show that checkMySequence could have helped avoid a widely discussed sequence register error in a model of SARS-CoV-2 RNA-dependent RNA polymerase that was originally detected thanks to a visual residue-by-residue inspection by members of the structural biology community.

Optimizing Roller Design to Improve Web Strain Uniformity in Roll-to-Roll Process

In this work, we investigated the compensation of strain deviation in the machine direction of a web in the roll-to-roll process. As flexible devices have become popular, many researchers have begun to study roll-to-roll processes for the mass-production of flexible devices at low cost. In the continuous roll-to-roll process, an electronic circuit pattern is printed on the web while the web is transferring. Due to tension and Poisson’s ratio, a non-uniform strain distribution can occur in the web. This strain distribution occurs mainly at the center of the web and causes a register error in the machine direction. In this work, we designed a roller to minimize the strain deviation. The design of the compensation roller was optimized using the design of experiments (DOE) methodology and analysis of variance (ANOVA), and the compensation performance was verified through experiments and simulations. According to the results of a comparative experiment conducted to confirm the correcting performance of the optimized roller, the strain deviation in the machine direction decreased by approximately 48% with the proposed roller compared to that of the conventional roller.

METHOD OF TEST CONTROL OF BOUNDARY PLAN DRAWED UP TO CORRECT OF A REGISTER ERROR

The article presents a test control method for a land survey plan drawn up to correct a register error.

SOFTWARE IMPLEMENTED HARDWARE-TRANSIENT FAULTS DETECTION

This paper examines a software implemented self-checking technique that is capable of detecting processorregisters' hardware-transient faults. The proposed approach is intended to detect run-time transient bit-errors in memory and processor status register. Error correction is not considered here. However, this low-cost approach is intended to be adopted in commodity systems that use ordinary off-the-shelf microprocessors, for the purpose of operational faults detection towards gaining fail-safe kind of fault tolerant system.

Machine Directional Register System Modeling for Shaft-Less Drive Gravure Printing Machines

In the latest type of gravure printing machines referred to as the shaft-less drive system, each gravure printing roller is driven by an individual servo motor, and all motors are electrically synchronized. The register error is regulated by a speed difference between the adjacent printing rollers. In order to improve the control accuracy of register system, an accurate mathematical model of the register system should be investigated for the latest machines. Therefore, the mathematical model of the machine directional register (MDR) system is studied for the multicolor gravure printing machines in this paper. According to the definition of the MDR error, the model is derived, and then it is validated by the numerical simulation and experiments carried out in the experimental setup of the four-color gravure printing machines. The results show that the established MDR system model is accurate and reliable.

Study on Registering Accuracy and Precision in Test of Printing Press

The registering precision is one of the most important specifications in printing press. Many factors that may influence the registering precision including the construction of the printing press in design, the assembly precision in manufacturing stage, adjustment in process of operation, the wear and tear of the machine when it is in use, crawl of blanket and extension of sheet are all considered comprehensively. The mathematical models are built up to describe the register error of press. The algorithm of accuracy and precision of sheet-feeding and sheet-transferring is introduced. With the aid of image technology, the coordinates of marks can be obtained automatically, and the registering accuracy and precision can also be calculated.

Curve Matching Algorithm Applied to Fabric Automatic Register Detection

With the register detection problems in the rotary screen printing process, the curve matching strategy was proposed to get the position vector of off-pattern-topping, on the basis of the proposed rotary screen printing machine automatic register detection system based on machine vision. In order to ensure the capture positions of the target edge image and the standard edge image is the same, Fourier-Mellin curve matching was used to match them to eliminate the shooting error;then the curve matching method, based on the Fourier transform shift theorem,was used to match the well-tuned target edge image and the standard edge image to detect the register error. The matching strategy eliminated the error, and the computation is small.The experiments show that its detection accuracy and the computing speed can meet the requirements of the fabric register detection on line.

Research on the register system modelling and control of gravure printing press

The register accuracy is the most important performance of gravure printing press. In order to improve the control accuracy of register, an accurate mathematical model of the register error and a high-performance controller should be researched. In this article, the mathematical model of the register error and the elimination of interference caused by the speed and tension of web by the feed-forward control scheme are studied. The established mathematical model and the feed-forward control scheme are validated by the numerical simulation and multi-colour gravure printing press test. The results show that the established register error model is accurate and reliable, and the designed feed-forward control scheme effectively reduces the register error caused by the tension and speed fluctuation.

The Detection System of Rotary Screen Printing Based on Machine Vision Technology

With the error detection problems in rotary screen printing, in this paper the machine vision technology is proposed to detect the design register error in the dyeing process, and the characteristics of the printing and dyeing contexture image are introduced, too. Combined with the features of " broken pattern " image, with the edge of the fabric image as characteristic curve, and through Fourier-mellin transform, the fabric image error detection is implemented; Finally, in the paper, the " broken pattern " error results show the feasibility of the scheme ,at the same time provide a theoretical basis for the further actual application.