Genetic Characterization of Common Eiders Breeding in the Yukon-Kuskokwim Delta, Alaska

We assessed population genetic subdivision among four colonies of Common Eiders (Somateria mollissima v-nigrum) breeding in the Yukon-Kuskokwim Delta (YKD), Alaska, using microsatellite genotypes and DNA sequences with differing modes of inheritance. Significant, albeit low, levels of genetic differentiation were observed between mainland populations and Kigigak Island for nuclear intron lamin A and mitochondrial DNA (mtDNA) control region. Intercolony variation in haplotypic frequencies also was observed at mtDNA. Positive growth signatures assayed from microsatellites, nuclear introns, and mtDNA indicate recent colonization of the YKD, and may explain the low levels of structuring observed. Gene flow estimates based on microsatellites, nuclear introns, and mtDNA suggest asymmetrical gene flow between mainland colonies and Kigigak Island, with more individuals on average dispersing from mainland populations to Kigigak Island than vice versa. The directionality of gene flow observed may be explained by the colonization of the YKD from northern glacial refugia or by YKD metapopulation dynamics.

Skeletal variability of the coral Favia gravida (Verrill, 1868) from Brazil

The skeletal variability of the coral Favia gravida, a species endemic to Brazil, was quantitatively described including populations from three locations: Tamandaré (state of Pernambuco), Abrolhos (state of Bahia), and Santa Cruz (state of Espírito Santo). Ten colonies were collected from each population and fourteen morphological characters were measured from ten corallites per colony. The results of univariate (among 14 skeletal characters, 7 showed p < 0.05) analysis provide evidence to suggest that F. gravida has considerable morphological plasticity, which may explain its ability to adapt to different ecological conditions. The species also displays polymorphism within and between colonies of each population. Intercolony variation within populations was relevant for most of the variables measured. Canonical discriminant analysis (r = 0.8648) showed that the population farthest offshore (Abrolhos) was distinct from the other two (Tamandaré and Santa Cruz), which have been affected by terrigenous sediments carried from the coast. Specimens from Santa Cruz displayed the highest degree of meandrinization.

Different Patterns of Parental Effort During Chick-Rearing by Female and Male Thick-Billed Murres (Uria Lomvia) at a Low-Arctic Colony

Using temperature loggers and radio telemetry, foraging and brooding behaviors of female and male Thick-billed Murres (Uria lomvia) were measured during chick rearing at the Gannet Islands, Labrador, to quantify parental roles during this crucial stage of reproduction. We recorded 2,725 foraging dives by 10 females and 7 males during 1,416 bird-hours of monitoring. Our study birds made between 21 and 107 dives per day between 0327 and 2220 hours, with 54% of females' dives and <1% of males' dives occurring in conditions of low illumination between sunset and sunrise. Males foraged mostly at midday and, overall, made longer and presumably deeper dives (mean duration 116.2 ± 37.6 s) than females (mean duration 84.1 ± 46.0 s). The maximum dive duration recorded was 240 s. Final dives of foraging trips were inferred to include dives during which chick meals (∼95% by number daubed shanny [Lumpenus maculatus]) were obtained. Those dives (mean duration 134.0 ± 36.1 s) and dives in final diving bouts prior to return to the breeding site (mean duration 139.1 ± 40.2 s) averaged longer than other dives, and longer in males than in females, but there was no difference between the sexes in their daily frequency. The modal travel time from the last dive in a feeding trip to arrival at the breeding site was 8 min (no difference between sexes), which represents a maximum travel distance at 65 km h−1 of 8.7 km, suggesting that foraging occurred close to the colony. Females spent 9 h day−1 (38% of day, mostly diurnal brooding) at breeding sites whereas males spent 16 h (67% of day, mostly nocturnal brooding). Taken together, these data indicated drastic differences in patterns of foraging and brooding behavior between male and female Thick-billed Murres, but suggest that individual and intercolony variation were also significant.

Isolation of Candida species on media with and without added fluconazole reveals high variability in relative growth susceptibility phenotypes.

Mouthwashes from human immunodeficiency virus-positive individuals were sampled for yeasts by direct plating on a differential agar medium with and without added fluconazole and via enrichment broths with and without added fluconazole. The colonies of the yeasts isolated were tested for relative growth in the presence of single concentrations of itraconazole and fluconazole. Among 258 culture plates containing yeasts obtained via different isolation routes from 86 yeast-positive samples, 33 (12.7%) of the plates showed unexpectedly high colony-to-colony variation in relative growth. Intercolony variation was seen in 41 (47.7%) of the 86 isolates when relative growth data were analyzed for all colonies of an isolate tested, regardless of the medium used for isolation. The prevalence of relative growth variability with the azoles was highest for Candida glabrata (100% of 13 isolates), followed by Candida krusei (60% of 5 isolates) and Candida albicans (40% of 53 isolates), and the visual patterns of variability seen in scatter plots of the data showed species specificity. Relative growth phenotypes generally tended to be stable for each yeast colony in subcultures, whether or not the medium used for subculture contained antifungal agents. DNA fingerprinting of stable and variable C. albicans isolates showed changes in band patterns detected with the probe Ca3, suggesting that the variability may have resulted from selection of different subtypes of the yeasts during the isolation procedure. These findings suggest that the yeasts isolated from single clinical samples were often not clonal in nature. The relative growth test revealed colony variability more readily than conventional susceptibility testing.

Species definition in heliolitine corals of the lower Douro Formation (Upper Silurian), Canadian Arctic

Specimens representingHeliolites diligensBondarenko, 1966,H.aff.H. luxarboreusYang, 1978, andH. tchernysheviBondarenko, 1966, are common andHeliolitessp. andStelliporellasp. are rare in diverse coral assemblages associated with lithistid sponge reefs in deep shelf or ramp limestone facies of the Douro Formation.Heliolites diligens, a more widely adapted, possibly “opportunist” species, occurs abundantly in lower diversity stromatoporoid/coral assemblages from nonreefal, shallower shelf limestone facies. Detailed systematic study of approximately contemporaneous populations of these Ludlovian heliolitid species shows that all are morphologically variable; assessment of this variability qualitatively and quantitatively is critical to species definition and recognition.Heliolites diligensis the most variable, with wide intercolony variation in septal development, corallite wall configuration, and spacing of horizontal skeletal elements. This apparently represents morphological plasticity rather than differences that can be ascribed to distinct species. Conspecificity of the more extreme and dissimilar variants can be inferred from study of large assemblages of coeval specimens. The other species, with more narrowly defined, discrete variation fields, are more readily distinguished from each other.

Some aspects of colony development in corals

Many peculiarities of growth and development in colonial metazoans are related to the fact that a true colony is a clone, composed of genetically identical individuals reproduced asexually from a single founding individual. In fossil colonies the close relationship of individuals may be difficult to prove, but it must be assumed for analysis to be meaningful.Intercolony variation is due to the interaction of genetic and environmental controls as in solitary organisms. Intracolony variation may be very limited because of the lack of genetic variation, but microenvironmental differences (situation within colony) may cause enough individual variation to mask the restrictive effect of genetic similarity.Study of the number of major septa in some Devonian solitary and colonial rugose corals shows significantly less variation within colonies than within “populations” or species of colonial or solitary forms. Greater variation within some colonies may result from either genetic or mechanical accidents. Septal number and diameter are closely correlated in populations and species, but analyses of individuals within colonies frequently show weak or no correlation. Available data suggest: 1) that septal-number variation may be genetically limited in rugose corals and 2) that the general assumption that number of septa is a function of diameter may be in error.