In Vitro Red Blood Cell Segregation in Sickle Cell Anemia

Red blood cells in sickle cell anemia (sRBC) are more heterogeneous in their physical properties than healthy red blood cells, spanning adhesiveness, rigidity, density, size, and shape. sRBC with increased adhesiveness to the vascular wall would trigger vaso-occlusive like complications, a hallmark of sickle cell anemia. We investigated whether segregation occurs among sRBC flowing in micron-sized channels and tested the impact of aggregation on segregation. Two populations of sRBC of different densities were separated, labeled, and mixed again. The mixed suspension was flowed within glass capillary tubes at different pressure-drops, hematocrit, and suspending media that promoted or not cell aggregation. Observations were made at a fixed channel position. The mean flow velocity was obtained by using the cells as tracking particles, and the cell depleted layer (CDL) by measuring the distance from the cell core border to the channel wall. The labeled sRBC were identified by stopping the flow and scanning the cells within the channel section. The tube hematocrit was estimated from the number of fluorescence cells identified in the field of view. In non-aggregating media, our results showed a heterogeneous distribution of sRBC according to their density: low-density sRBC population remained closer to the center of the channel, while the densest cells segregated towards the walls. There was no impact of the mean flow velocity and little impact of hematocrit. This segregation heterogeneity could influence the ability of sRBC to adhere to the vascular wall and slow down blood flow. However, promoting aggregation inhibited segregation while CDL thickness was enhanced by aggregation, highlighting a potential protective role against vaso-occlusion in patients with sickle cell anemia.

Drag model validation of slurry pipeline using CFD

A number of drag models have been suggested for the interaction of fluid particles in slurry flow over the previous centuries. It is necessary to examine the correctness and applicability of these models in the slurry transportation. Based on this concept, a comparative analysis of the different drag models is performed for the 0.0549 m diameter slurry pipeline. The research is carried out by using three drag models: Syamlal-obrien, Schiller-naumann and Gidaspow due to their accessibility in the Fluent commercial software. The simulation is performed at mean flow velocity range, Vm= 2–5 ms-1 and solid concentration range, Cvf = 10– 20% (by volume) using computational drag models. The simulated outcomes for solid particle size 440 μm having density 2470 kg/m3 are recorded using Eulerian two-phase model with selected drag models in the computational domain. It has been found that the Eulerian two-phase model with Syamlal O’brien drag model gives the accurate and meticulous results with the published data in the literature. Finally, the simulated outcomes of solid concentration contours, solid concentration profiles and pressure drop are predicted at distinct velocity and solid concentration range for chosen drag models.

The role of increasing riverbank vegetation density on flow dynamics across an asymmetrical channel

Over the last two decades, the role of vegetation in the environmental and ecological restoration of surface water bodies has received much attention. In this context, the momentum exchange between the flow through the main channel and the riparian zone is a key mechanism. The primary goal of this study is to investigate the role of bank vegetation density on flow dynamics across the whole channel. This experimental study presents the major findings from a series of flow measurements across a channel having a sloping bank with vegetation at varying densities. The experiments are conducted under the same, uniform flow and fixed bed conditions, for a range of six linear and rectilinear arrangements of incremental streambank vegetation densities. A set of ten velocity profiles is obtained across the test cross-section of the channel, including the riverbank, for each vegetation density. These flow measurements are analyzed to derive roughness coefficients, which are related to the bulk flow velocities through the main channel and the riverbank and discuss the redistribution of flow velocities. An approximate doubling for the estimates of time-averaged boundary shear stress at the main channel, is observed for the case of no to dense vegetation, which enable further discussing implications for the stability of bed surface material. It is found that the vegetation arrangement, in addition to vegetation density, can have a strong impact in modifying the mean flow velocity at the main channel, for low riparian densities (φ < 0.6%). Highlights Flow dynamics are measured across the whole channel, including the vegetated riverbank. As stem density increases, mean flow velocity in the main channel increases while mean flow at the riverbank decreases. The arrangement of riparian vegetation can be as important as that of the density, in modifying the mean flow field of the main channel, for low riparian densities. Bed shear stresses at the main channel are estimated to increase with riverbank vegetation, reducing the stability of the stream’s bed surface.

Effect of dissipation in rapid-gravitational granular flows

We investigate numerically high speed granular flows down an incline and focus our attention on the influence of the restitution coefficient e of binary collisions on the nature of the flow regimes. We show in particular that e plays a major role in rapid flows. Decreasing e leads in general to denser flows but also quicker flows. The increase of the mean flow velocity with decreasing e is explained as the result of the clustering instability which produces a dense and cold core moving very fast as a plug.

Effects of Mean Inflow Velocity and Droplet Diameter on the Propagation of Turbulent V-Shaped Flames in Droplet-Laden Mixtures

Three-dimensional carrier phase Direct Numerical Simulations of V-shaped n-heptane spray flames have been performed for different initially mono-sized droplet diameters to investigate the influence of mean flow velocity on the burning rate and flame structure at different axial locations from the flame holder. The fuel is supplied as liquid droplets through the inlet and an overall (i.e., liquid + gaseous) equivalence ratio of unity is retained in the unburned gas. Additionally, turbulent premixed stoichiometric V-shaped n-heptane flames under the same turbulent flow conditions have been simulated to distinguish the differences in combustion behaviour of the pure gaseous phase premixed combustion in comparison to the corresponding behaviour in the presence of liquid n-heptane droplets. It has been found that reacting gaseous mixture burns predominantly under fuel-lean mode and the availability of having fuel-lean mixture increases with increasing mean flow velocity. The extent of flame wrinkling for droplet cases has been found to be greater than the corresponding gaseous premixed flames due to flame-droplet-interaction, which is manifested by dimples on the flame surface, and this trend strengthens with increasing droplet diameter. As the residence time of the droplets within the flame decreases with increasing mean inflow velocity, the droplets can survive for larger axial distances before the completion of their evaporation for the cases with higher mean inflow velocity and this leads to greater extents of flame-droplet interaction and droplet-induced flame wrinkling. Mean inflow velocity, droplet diameter and the axial distance affect the flame brush thickness. The flame brush thickens with increasing droplet diameter for the cases with higher mean inflow velocity due to the predominance of fuel-lean gaseous mixture within the flame. However, an opposite behaviour has been observed for the cases with lower mean inflow velocity where the smaller extent of flame wrinkling due to smaller values of integral length scale to flame thickness ratio arising from higher likelihood of fuel-lean combustion for larger droplets dominates over the thickening of the flame front. It has been found that the major part of the heat release arises due to premixed mode of combustion for all cases but the contribution of non-premixed mode of combustion to the total heat release has been found to increase with increasing mean inflow velocity and droplet diameter. The increase in the mean inflow velocity yields an increase in the mean values of consumption and density-weighted displacement speed for the droplet cases but leads to a decrease in turbulent burning velocity. By contrast, an increase in droplet diameter gives rise to decreases in turbulent burning velocity, and the mean values of consumption and density-weighted displacement speeds. Detailed physical explanations have been provided to explain the observed mean inflow velocity and droplet diameter dependences of the flame propagation behaviour.

River Flow Monitoring by Sentinel-3 OLCI and MODIS: Comparison and Combination

The monitoring of rivers by satellite is an up-to-date subject in hydrological studies as confirmed by the interest of space agencies to finance specific missions that respond to the quantification of surface water flows. We address the problem by using multi-spectral sensors, in the near-infrared (NIR) band, correlating the reflectance ratio between a dry and a wet pixel extracted from a time series of images, the C/M ratio, with five river flow-related variables: water level, river discharge, flow area, mean flow velocity and surface width. The innovative aspect of this study is the use of the Ocean and Land Colour Instrument (OLCI) on board Sentinel-3 satellites, compared to the Moderate Resolution Imaging Spectroradiometer (MODIS) used in previous studies. Our results show that the C/M ratio from OLCI and MODIS is more correlated with the mean flow velocity than with other variables. To improve the number of observations, OLCI and MODIS products are combined into multi-mission time series. The integration provides good quality data at around daily resolution, appropriate for the analysis of the Po River investigated in this study. Finally, the combination of only MODIS products outperforms the other configurations with a frequency slightly lower (~1.8 days).

Three-dimensional spatial structure of the macro-pores and flow simulation in anthracite coal based on X-ray μ-CT scanning data

The three-dimensional (3D) structures of pores directly affect the CH4 flow. Therefore, it is very important to analyze the 3D spatial structure of pores and to simulate the CH4 flow with the connected pores as the carrier. The result shows that the equivalent radius of pores and throats are 1–16 μm and 1.03–8.9 μm, respectively, and the throat length is 3.28–231.25 μm. The coordination number of pores concentrates around three, and the intersection point between the connectivity function and the X-axis is 3–4 μm, which indicate the macro-pores have good connectivity. During the single-channel flow, the pressure decreases along the direction of CH4 flow, and the flow velocity of CH4 decreases from the pore center to the wall. Under the dual-channel and the multi-channel flows, the pressure also decreases along the CH4 flow direction, while the velocity increases. The mean flow pressure gradually decreases with the increase of the distance from the inlet slice. The change of mean flow pressure is relatively stable in the direction horizontal to the bedding plane, while it is relatively large in the direction perpendicular to the bedding plane. The mean flow velocity in the direction horizontal to the bedding plane (Y-axis) is the largest, followed by that in the direction horizontal to the bedding plane (X-axis), and the mean flow velocity in the direction perpendicular to the bedding plane is the smallest.