A Case of Malaria Patient with SARS-CoV-2 Antibody False-Positive

Background: The SARS-CoV-2 antibody detection are used to diagnose or exclude suspected COVID-19 patients as a supplement to nucleic acid detection. False-positive results of SARS-CoV-2 antibody have been reported but rarely associated with malaria. A case of malaria patient with SARS-CoV-2 antibody false-positive is described.Case presentation: A 24 year-old male returned from Côte d’Ivoire was diagnosed Plasmodium falciparum by Malaria rapid diagnostic test. The patient had suspicious exposure to COVID-19. His SARS-CoV-2 IgM antibody was positive one day before admission and turned negative on the 18th day of admission, while the IgG antibody and nasopharyngeal swabs SARS-Cov-2 nucleic acid had been negative. Conclusion: Malaria might cause false positive for SARS-CoV-2 IgM antibody. A careful interpretation of the SARS-CoV-2 antibody result is useful to avoid wasting medical resources especially malaria-endemic areas.

HUBUNGAN ANTARA FAKTOR SOSIODEMOGRAFI, SOSIOEKONOMI DAN SOSIOBUDAYA DENGAN PENCARIAN PENGOBATAN MALARIA DI PUSKESMAS KALIGESING KABUPATEN PURWOREJO

Malaria is one of public health problems. WHO reports that there are 109 endemic countries of malaria with 250 million cases and 1 million of death in the world every year. Indonesia is one of endemic country with malaria. Malaria cases are widely found in Indonesia with 30.000 of death every year. Purworejo is one of endemic area of malaria in Indonesia. There is increasing of malaria cses every year in Purworejo. Furthermore, people in Purworejo assume that malaria is so common disease that they more believe to choose traditional treatment as effort to cure and heal malaria that they get. The research was observational-analitic research with cross sectional design. Number of sample were 70 people.Sample of research consisted of two group, which are malaria patient who got medical treatment and malaria patient who got traditional treatment. Malaria patients who got medical treatment were choosen by data from public health center. Meanwhile malaria patients who got traditional treatment were choosen based on public figure’s guidance in research place. Analysis of relationship between independence and dependence variable was tested by Chi-Square test. Meanwhile analysis of most influential variable applied logistic regression. There were four variables related with malaria seeking care, gender, belief, knowledge and perception of seriousness. Variable that simultaneously affected malaria seeking care were knowledge and perception of seriousness. Meanwhile the most influential variable was perception of seriousness. It is recommended to Department of Health to create plans and implement program about communication, information and education of malaria, especially about malaria seeking care, with inviting religionist and public figure in research place. Beside that, it is also expected to people in Purworejo to improve their knowledge about malaria, especially effort of prevention and treatment. Keyword : Health Seeking Behavior, Malaria

The Prevalence of Insecticide Treated Net/malaria :cross section study (Preprint)

BACKGROUND ABSTRACT Introduction a study on prevalence of ITN use was carried out in Buchi community Kitwe Zambia from August to October 2019 OBJECTIVE Prevalence of ITN in BUchi METHODS Methodology: This was a cross sectional study design. A structured questionnaire was used to ascertain ownership and utilization and oral interviews, 200 households were targeted 844 individual covered across the 200 households. Data was analyzed with SPSS version 23. RESULTS ABSTRACT Introduction a study on prevalence of ITN use was carried out in Buchi community Kitwe Zambia from August to October 2019 Methodology: This was a cross sectional study design. A structured questionnaire was used to ascertain ownership and utilization and oral interviews, 200 households were targeted 844 individual covered across the 200 households. Data was analyzed with SPSS version 23. Findings: household ownership of at least an ITN was 52% and individual utilization at 37.6%, with 0.825 ITN/households and 0.195ITN /individual. Malaria prevalence of 52.4% /household and a 12.4% of the population. With 47.6% malaria patient coming from households with ITN and 60% of households with ITN have insufficient coverage.61% of malaria patient were female and 31 % male. however there was no significant relation between Gender and malaria prevalence in study area (p value was >0.05). Malaria cases distribution with age groups, 0-15yrs old represented 49.5%, 16-30 yrs., was at 27.6% and the over 30 yrs. case were at 22.9% .use of other preventive measures 23% used mosquito repellent ,others methods 1% with those not using any other methods 76%. CONCLUSIONS Conclusion The study showed clearly that malaria still poses a problem .the prevalence rate of malaria was still high 12.4% of the population and 52.4% of households. With high prevalence of malaria of 49.5% for 0-15yrs.the difference between ownership 52% and Utilization 37.6% showed that even household with ITN, the ITN were not sufficient.60% of households with ITN, the ITN were not sufficient for all occupants

Deteksi Protein 270 kDa Plasmodium falciparum Isolat Malang pada Membran Eritrosit Penderita Malaria dengan Komplikasi

Erythrocyte which is infected by Plasmodium falciparum will have various changes on its architecture, affinity, and biomolecular. Beside that, the infected erythrocyte also forms a knob at its surface. This knob are contained with various parasite proteins, one of them is Plasmodium falciparum erythrocyte membrane protein-1 (PfEMP-1). Our previous study had been identified a protein with molecular weight 270 kDa at P. falciparum infected erythrocyte from Malang isolate that was playing a role in cytoadherence process. The aim of this study was to detect the possibility of 270 kDa protein expression at complicated malaria falciparum patient erythrocyte membrane. The method that used was immunocytochemistry with polyclonal antibody to 270 kDa protein. The results showed that two (2) erythrocyte samples from healthy people as control had negative reaction, and so did with five (5) erythrocyte samples of uncomplicated malaria patient, but there was positive reaction that shown at two (2) samples of complicated malaria patient erythrocyte. It can be concluded from the results that 270 kDa membrane protein of P. falciparum infected erythrocyte in complicated malaria patient might be a PfEMP-1. This protein can be detected by immunocytochemistry method using polyclonal antibody and can be used for the candidate of complicated malaria diagnostic Keywords: Plasmodium falciparum, 270 kDA protein, immunocytochemistry, polyclonal antibody